YDR491C_d

Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR491C / YDR491C

Click on Significant Values for Screen Details ID:SGTC_317|Compound:4495-0002|FD-Score:-3.20|P-value:3.00E-4 ID:SGTC_325|Compound:2810-4341|FD-Score:3.74|P-value:2.13E-5 ID:SGTC_327|Compound:1273-0057|FD-Score:-3.07|P-value:5.01E-4 ID:SGTC_331|Compound:k048-0133|FD-Score:-2.95|P-value:7.99E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:3.41|P-value:9.15E-5 ID:SGTC_382|Compound:3229-0966|FD-Score:3.69|P-value:2.64E-5 ID:SGTC_413|Compound:propylparaben|FD-Score:2.85|P-value:8.79E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:2.92|P-value:6.67E-4 ID:SGTC_527|Compound:4476-4215|FD-Score:3.28|P-value:1.61E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:3.53|P-value:5.48E-5 ID:SGTC_613|Compound:3914-0030|FD-Score:-3.92|P-value:1.27E-5 ID:SGTC_669|Compound:0108-0021|FD-Score:3.08|P-value:3.57E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.22|P-value:2.82E-4 ID:SGTC_886|Compound:0866-0150|FD-Score:2.87|P-value:8.00E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.06|P-value:5.29E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:-2.96|P-value:7.75E-4 ID:SGTC_1309|Compound:pyrazolanthrone|FD-Score:3.03|P-value:4.36E-4 ID:SGTC_1331|Compound:1472-0049|FD-Score:-2.96|P-value:7.62E-4 ID:SGTC_1338|Compound:1486-1223|FD-Score:2.93|P-value:6.49E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:4.65|P-value:1.87E-7 ID:SGTC_1698|Compound:5-fluorocytosine|FD-Score:3.47|P-value:7.14E-5 ID:SGTC_1712|Compound:st032288|FD-Score:6.46|P-value:8.80E-13 ID:SGTC_1717|Compound:st033143|FD-Score:3.31|P-value:1.43E-4 ID:SGTC_1747|Compound:st037496|FD-Score:-3.09|P-value:4.72E-4 ID:SGTC_1754|Compound:st042065|FD-Score:4.83|P-value:6.62E-8 ID:SGTC_1760|Compound:st045453|FD-Score:9.00|P-value:5.33E-23 ID:SGTC_1782|Compound:st039423|FD-Score:-4.10|P-value:5.35E-6 ID:SGTC_1786|Compound:berberine|FD-Score:-5.07|P-value:2.46E-8 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-3.36|P-value:1.55E-4 ID:SGTC_1800|Compound:st050079|FD-Score:4.12|P-value:3.33E-6 ID:SGTC_1803|Compound:st050068|FD-Score:5.03|P-value:1.88E-8 ID:SGTC_1872|Compound:amphotericin b|FD-Score:3.13|P-value:3.00E-4 ID:SGTC_1885|Compound:st057546|FD-Score:2.86|P-value:8.37E-4 ID:SGTC_2021|Compound:4019055|FD-Score:4.48|P-value:4.77E-7 ID:SGTC_2385|Compound:9074692|FD-Score:-2.90|P-value:9.37E-4 ID:SGTC_2625|Compound:euphol|FD-Score:3.48|P-value:6.89E-5 ID:SGTC_2693|Compound:st077581|FD-Score:3.65|P-value:3.11E-5 ID:SGTC_2794|Compound:7785382|FD-Score:2.82|P-value:9.85E-4 ID:SGTC_2956|Compound:9076196|FD-Score:-3.54|P-value:7.23E-5 ID:SGTC_3206|Compound:9114350|FD-Score:-3.71|P-value:3.39E-5 ID:SGTC_3336|Compound:9143619|FD-Score:2.87|P-value:8.04E-4 ID:SGTC_317|Compound:4495-0002|FD-Score:-3.20|P-value:3.00E-4 ID:SGTC_325|Compound:2810-4341|FD-Score:3.74|P-value:2.13E-5 ID:SGTC_327|Compound:1273-0057|FD-Score:-3.07|P-value:5.01E-4 ID:SGTC_331|Compound:k048-0133|FD-Score:-2.95|P-value:7.99E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:3.41|P-value:9.15E-5 ID:SGTC_382|Compound:3229-0966|FD-Score:3.69|P-value:2.64E-5 ID:SGTC_413|Compound:propylparaben|FD-Score:2.85|P-value:8.79E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:2.92|P-value:6.67E-4 ID:SGTC_527|Compound:4476-4215|FD-Score:3.28|P-value:1.61E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:3.53|P-value:5.48E-5 ID:SGTC_613|Compound:3914-0030|FD-Score:-3.92|P-value:1.27E-5 ID:SGTC_669|Compound:0108-0021|FD-Score:3.08|P-value:3.57E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.22|P-value:2.82E-4 ID:SGTC_886|Compound:0866-0150|FD-Score:2.87|P-value:8.00E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.06|P-value:5.29E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:-2.96|P-value:7.75E-4 ID:SGTC_1309|Compound:pyrazolanthrone|FD-Score:3.03|P-value:4.36E-4 ID:SGTC_1331|Compound:1472-0049|FD-Score:-2.96|P-value:7.62E-4 ID:SGTC_1338|Compound:1486-1223|FD-Score:2.93|P-value:6.49E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:4.65|P-value:1.87E-7 ID:SGTC_1698|Compound:5-fluorocytosine|FD-Score:3.47|P-value:7.14E-5 ID:SGTC_1712|Compound:st032288|FD-Score:6.46|P-value:8.80E-13 ID:SGTC_1717|Compound:st033143|FD-Score:3.31|P-value:1.43E-4 ID:SGTC_1747|Compound:st037496|FD-Score:-3.09|P-value:4.72E-4 ID:SGTC_1754|Compound:st042065|FD-Score:4.83|P-value:6.62E-8 ID:SGTC_1760|Compound:st045453|FD-Score:9.00|P-value:5.33E-23 ID:SGTC_1782|Compound:st039423|FD-Score:-4.10|P-value:5.35E-6 ID:SGTC_1786|Compound:berberine|FD-Score:-5.07|P-value:2.46E-8 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-3.36|P-value:1.55E-4 ID:SGTC_1800|Compound:st050079|FD-Score:4.12|P-value:3.33E-6 ID:SGTC_1803|Compound:st050068|FD-Score:5.03|P-value:1.88E-8 ID:SGTC_1872|Compound:amphotericin b|FD-Score:3.13|P-value:3.00E-4 ID:SGTC_1885|Compound:st057546|FD-Score:2.86|P-value:8.37E-4 ID:SGTC_2021|Compound:4019055|FD-Score:4.48|P-value:4.77E-7 ID:SGTC_2385|Compound:9074692|FD-Score:-2.90|P-value:9.37E-4 ID:SGTC_2625|Compound:euphol|FD-Score:3.48|P-value:6.89E-5 ID:SGTC_2693|Compound:st077581|FD-Score:3.65|P-value:3.11E-5 ID:SGTC_2794|Compound:7785382|FD-Score:2.82|P-value:9.85E-4 ID:SGTC_2956|Compound:9076196|FD-Score:-3.54|P-value:7.23E-5 ID:SGTC_3206|Compound:9114350|FD-Score:-3.71|P-value:3.39E-5 ID:SGTC_3336|Compound:9143619|FD-Score:2.87|P-value:8.04E-4

Top fitness defect scores for YDR491C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1760 st045453 9.00 10.4 µM 5.33E-23 NEO1-PIK1 40 1.10E-19
2 SGTC_1712 st032288 6.46 53.4 µM 8.80E-13 calcium & mitochondrial duress 66 5.10E-11
3 SGTC_1803 st050068 5.03 52.4 µM 1.88E-8 NEO1-PIK1 114 2.40E-7
4 SGTC_1754 st042065 4.83 16.1 µM 6.62E-8 NEO1-PIK1 136 6.97E-7
5 SGTC_1369 1866-0067 4.65 208.0 µM 1.87E-7 RPP1 & pyrimidine depletion 21 1.68E-6
6 SGTC_2021 4019055 4.48 173.0 µM 4.77E-7 endomembrane recycling 94 3.72E-6
7 SGTC_1800 st050079 4.12 29.1 µM 3.33E-6 NEO1-PIK1 113 1.94E-5
8 SGTC_325 2810-4341 3.74 181.0 µM 2.13E-5 amide catabolism 12 9.35E-5
9 SGTC_382 3229-0966 3.69 23.2 µM 2.64E-5 29 1.12E-4
10 SGTC_2693 st077581 3.65 40.7 µM 3.11E-5 49 1.29E-4
11 SGTC_528 3492-0019 3.53 172.0 µM 5.48E-5 calcium & mitochondrial duress 254 2.09E-4
12 SGTC_2625 euphol 3.48 100.0 µM 6.89E-5 46 2.54E-4
13 SGTC_1698 5-fluorocytosine 3.47 1.1 µM 7.14E-5 exosome 15 2.62E-4
14 SGTC_358 0075-0058 3.41 9.4 µM 9.15E-5 91 3.23E-4
15 SGTC_1717 st033143 3.31 58.0 µM 1.43E-4 73 4.73E-4
16 SGTC_527 4476-4215 3.28 268.0 µM 1.61E-4 PDR1 63 5.22E-4
17 SGTC_1872 amphotericin b 3.13 148.0 nM 3.00E-4 RNA pol III & RNase P/MRP 53 8.85E-4
18 SGTC_669 0108-0021 3.08 26.2 µM 3.57E-4 fatty acid desaturase (OLE1) 148 0.00103
19 SGTC_1309 pyrazolanthrone 3.03 13.4 µM 4.36E-4 54 0.00122
20 SGTC_1338 1486-1223 2.93 76.0 µM 6.49E-4 64 0.00171

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.438 3.87E-157 YPR091C NVJ2 hom Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments
0.401 5.97E-130 YML097C VPS9 hom A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin
0.387 4.65E-120 YPR092W_d YPR092W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.367 1.04E-107 YLR020C YEH2 hom Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism
0.364 1.69E-105 YDR492W IZH1 hom Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc
0.352 3.12E-98 YGL035C MIG1 hom Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion
0.347 1.77E-95 YHR108W GGA2 hom Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin
0.318 1.74E-79 YDR084C TVP23 hom Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
0.314 2.02E-77 YPL145C KES1 hom One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication
0.311 3.92E-76 YIL170W HXT12 hom Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family
0.305 3.25E-73 YAR042W SWH1 hom Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction
0.289 8.45E-66 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.282 1.92E-62 YNL183C NPR1 hom Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex
0.279 5.39E-61 YNL267W PIK1 het Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.277 2.88E-60 YER120W SCS2 hom Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication