YDR496C / PUF6

Pumilio-homology domain protein that binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; also co-sediments with the 60S ribosomal subunit and is required for its biogenesis

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR496C / PUF6

Click on Significant Values for Screen Details ID:SGTC_59|Compound:0330-0160|FD-Score:3.21|P-value:2.66E-4 ID:SGTC_115|Compound:0527-0192|FD-Score:3.90|P-value:1.33E-5 ID:SGTC_143|Compound:4032-1194|FD-Score:3.96|P-value:1.03E-5 ID:SGTC_145|Compound:3737-0113|FD-Score:5.12|P-value:1.88E-8 ID:SGTC_158|Compound:k038-0087|FD-Score:-2.99|P-value:7.65E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:2.87|P-value:9.85E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:3.18|P-value:3.04E-4 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:3.82|P-value:1.95E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.07|P-value:4.73E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:3.28|P-value:2.01E-4 ID:SGTC_552|Compound:k242-0005|FD-Score:3.58|P-value:5.88E-5 ID:SGTC_618|Compound:k072-0232|FD-Score:-4.55|P-value:6.99E-7 ID:SGTC_621|Compound:3584-0052|FD-Score:-4.56|P-value:6.56E-7 ID:SGTC_627|Compound:1391-0720|FD-Score:-2.99|P-value:7.65E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:-4.08|P-value:7.45E-6 ID:SGTC_1212|Compound:0520-0165|FD-Score:3.22|P-value:2.57E-4 ID:SGTC_1328|Compound:1441-0025|FD-Score:-4.03|P-value:9.61E-6 ID:SGTC_1333|Compound:1482-0047|FD-Score:4.15|P-value:3.94E-6 ID:SGTC_1381|Compound:2324-0066|FD-Score:3.04|P-value:5.29E-4 ID:SGTC_1672|Compound:st016598|FD-Score:-3.68|P-value:4.69E-5 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:-3.00|P-value:7.41E-4 ID:SGTC_1748|Compound:st037455|FD-Score:3.12|P-value:3.81E-4 ID:SGTC_1825|Compound:st052143|FD-Score:3.25|P-value:2.32E-4 ID:SGTC_2050|Compound:5263056|FD-Score:3.45|P-value:9.92E-5 ID:SGTC_2157|Compound:5649910|FD-Score:3.63|P-value:4.59E-5 ID:SGTC_2328|Compound:6201408|FD-Score:3.42|P-value:1.15E-4 ID:SGTC_2329|Compound:6138490|FD-Score:3.83|P-value:1.86E-5 ID:SGTC_2332|Compound:7982454|FD-Score:-2.97|P-value:8.53E-4 ID:SGTC_2511|Compound:berberine|FD-Score:-3.03|P-value:6.79E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:-3.67|P-value:5.07E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.82|P-value:2.58E-5 ID:SGTC_2589|Compound:lobaric acid|FD-Score:3.40|P-value:1.24E-4 ID:SGTC_2690|Compound:usnic acid|FD-Score:4.84|P-value:1.01E-7 ID:SGTC_2711|Compound:st079103|FD-Score:2.91|P-value:8.53E-4 ID:SGTC_2836|Compound:9010338|FD-Score:3.20|P-value:2.82E-4 ID:SGTC_2966|Compound:9084041|FD-Score:-3.69|P-value:4.66E-5 ID:SGTC_2970|Compound:9090938|FD-Score:3.53|P-value:7.31E-5 ID:SGTC_3190|Compound:9110366|FD-Score:3.21|P-value:2.72E-4 ID:SGTC_3191|Compound:9110352|FD-Score:-3.16|P-value:4.10E-4 ID:SGTC_3192|Compound:9110362|FD-Score:-3.45|P-value:1.26E-4 ID:SGTC_3355|Compound:9151058|FD-Score:-3.10|P-value:5.12E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:3.21|P-value:2.66E-4 ID:SGTC_115|Compound:0527-0192|FD-Score:3.90|P-value:1.33E-5 ID:SGTC_143|Compound:4032-1194|FD-Score:3.96|P-value:1.03E-5 ID:SGTC_145|Compound:3737-0113|FD-Score:5.12|P-value:1.88E-8 ID:SGTC_158|Compound:k038-0087|FD-Score:-2.99|P-value:7.65E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:2.87|P-value:9.85E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:3.18|P-value:3.04E-4 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:3.82|P-value:1.95E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.07|P-value:4.73E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:3.28|P-value:2.01E-4 ID:SGTC_552|Compound:k242-0005|FD-Score:3.58|P-value:5.88E-5 ID:SGTC_618|Compound:k072-0232|FD-Score:-4.55|P-value:6.99E-7 ID:SGTC_621|Compound:3584-0052|FD-Score:-4.56|P-value:6.56E-7 ID:SGTC_627|Compound:1391-0720|FD-Score:-2.99|P-value:7.65E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:-4.08|P-value:7.45E-6 ID:SGTC_1212|Compound:0520-0165|FD-Score:3.22|P-value:2.57E-4 ID:SGTC_1328|Compound:1441-0025|FD-Score:-4.03|P-value:9.61E-6 ID:SGTC_1333|Compound:1482-0047|FD-Score:4.15|P-value:3.94E-6 ID:SGTC_1381|Compound:2324-0066|FD-Score:3.04|P-value:5.29E-4 ID:SGTC_1672|Compound:st016598|FD-Score:-3.68|P-value:4.69E-5 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:-3.00|P-value:7.41E-4 ID:SGTC_1748|Compound:st037455|FD-Score:3.12|P-value:3.81E-4 ID:SGTC_1825|Compound:st052143|FD-Score:3.25|P-value:2.32E-4 ID:SGTC_2050|Compound:5263056|FD-Score:3.45|P-value:9.92E-5 ID:SGTC_2157|Compound:5649910|FD-Score:3.63|P-value:4.59E-5 ID:SGTC_2328|Compound:6201408|FD-Score:3.42|P-value:1.15E-4 ID:SGTC_2329|Compound:6138490|FD-Score:3.83|P-value:1.86E-5 ID:SGTC_2332|Compound:7982454|FD-Score:-2.97|P-value:8.53E-4 ID:SGTC_2511|Compound:berberine|FD-Score:-3.03|P-value:6.79E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:-3.67|P-value:5.07E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.82|P-value:2.58E-5 ID:SGTC_2589|Compound:lobaric acid|FD-Score:3.40|P-value:1.24E-4 ID:SGTC_2690|Compound:usnic acid|FD-Score:4.84|P-value:1.01E-7 ID:SGTC_2711|Compound:st079103|FD-Score:2.91|P-value:8.53E-4 ID:SGTC_2836|Compound:9010338|FD-Score:3.20|P-value:2.82E-4 ID:SGTC_2966|Compound:9084041|FD-Score:-3.69|P-value:4.66E-5 ID:SGTC_2970|Compound:9090938|FD-Score:3.53|P-value:7.31E-5 ID:SGTC_3190|Compound:9110366|FD-Score:3.21|P-value:2.72E-4 ID:SGTC_3191|Compound:9110352|FD-Score:-3.16|P-value:4.10E-4 ID:SGTC_3192|Compound:9110362|FD-Score:-3.45|P-value:1.26E-4 ID:SGTC_3355|Compound:9151058|FD-Score:-3.10|P-value:5.12E-4

Top fitness defect scores for YDR496C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_145 3737-0113 5.12 38.4 µM 1.88E-8 DNA intercalators 39 1.52E-7
2 SGTC_2690 usnic acid 4.84 100.0 µM 1.01E-7 13 6.58E-7
3 SGTC_1333 1482-0047 4.15 286.0 µM 3.94E-6 13 1.63E-5
4 SGTC_143 4032-1194 3.96 15.7 µM 1.03E-5 66 3.78E-5
5 SGTC_115 0527-0192 3.90 112.0 µM 1.33E-5 72 4.75E-5
6 SGTC_2329 6138490 3.83 200.0 µM 1.86E-5 36 6.34E-5
7 SGTC_402 epigallocatechin gallate 3.82 10.0 µM 1.95E-5 31 6.61E-5
8 SGTC_2157 5649910 3.63 96.4 µM 4.59E-5 30 1.40E-4
9 SGTC_552 k242-0005 3.58 476.0 µM 5.88E-5 29 1.74E-4
10 SGTC_2970 9090938 3.53 41.9 µM 7.31E-5 RNA processing & uracil transport 60 2.11E-4
11 SGTC_2050 5263056 3.45 171.0 µM 9.92E-5 fatty acid desaturase (OLE1) 70 2.75E-4
12 SGTC_2328 6201408 3.42 200.0 µM 1.15E-4 39 3.14E-4
13 SGTC_2589 lobaric acid 3.40 100.0 µM 1.24E-4 41 3.34E-4
14 SGTC_513 4245-1575 3.28 41.7 µM 2.01E-4 unfolded protein response 164 5.11E-4
15 SGTC_1825 st052143 3.25 62.6 µM 2.32E-4 35 5.81E-4
16 SGTC_1212 0520-0165 3.22 585.0 µM 2.57E-4 69 6.34E-4
17 SGTC_59 0330-0160 3.21 1.3 µM 2.66E-4 176 6.53E-4
18 SGTC_3190 9110366 3.21 49.5 µM 2.72E-4 33 6.66E-4
19 SGTC_2836 9010338 3.20 19.5 µM 2.82E-4 RPP1 & pyrimidine depletion 69 6.89E-4
20 SGTC_359 0081-0058 3.18 15.9 µM 3.04E-4 60S ribosome export 90 7.36E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.164 1.10E-21 YHR149C SKG6 hom Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p
0.161 5.28E-21 YDR401W_d YDR401W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.132 2.05E-14 YEL064C AVT2 hom Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
0.130 4.11E-14 YBL008W-A_p YBL008W-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.129 6.91E-14 YGL002W ERP6 hom Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication
0.128 1.03E-13 YFL022C FRS2 het Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
0.116 1.92E-11 YGL118C_d YGL118C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.115 2.06E-11 YNL124W NAF1 het RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p
0.113 6.17E-11 YOR344C TYE7 hom Serine-rich protein that contains a basic-helix-loop-helix (bHLH) DNA binding motif; binds E-boxes of glycolytic genes and contributes to their activation; may function as a transcriptional activator in Ty1-mediated gene expression
0.112 9.07E-11 YDL096C_d OPI6_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential
0.109 2.40E-10 YBL082C ALG3 hom Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins
0.108 4.36E-10 YEL017C-A PMP2 hom Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication
0.107 5.24E-10 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.106 7.99E-10 YPR126C_d YPR126C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.106 7.87E-10 YNL264C PDR17 hom Phosphatidylinositol transfer protein (PITP), downregulates Plb1p-mediated turnover of phosphatidylcholine, found in the cytosol and microsomes, homologous to Pdr16p, deletion affects phospholipid composition