YDR515W / SLF1

RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR515W / SLF1

Click on Significant Values for Screen Details ID:SGTC_50|Compound:0929-0078|FD-Score:-3.99|P-value:3.21E-5 ID:SGTC_52|Compound:0329-0193|FD-Score:-4.47|P-value:3.73E-6 ID:SGTC_58|Compound:0957-0393|FD-Score:6.37|P-value:1.20E-10 ID:SGTC_85|Compound:0868-0259|FD-Score:-3.09|P-value:9.68E-4 ID:SGTC_91|Compound:0417-1751|FD-Score:3.32|P-value:4.90E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:4.99|P-value:3.55E-7 ID:SGTC_235|Compound:0330-0087|FD-Score:3.12|P-value:9.88E-4 ID:SGTC_264|Compound:promethazine|FD-Score:-3.28|P-value:5.00E-4 ID:SGTC_271|Compound:rotenone|FD-Score:-3.25|P-value:5.58E-4 ID:SGTC_298|Compound:1391-0729|FD-Score:3.16|P-value:8.53E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:4.38|P-value:6.89E-6 ID:SGTC_679|Compound:0422-0091|FD-Score:3.15|P-value:8.77E-4 ID:SGTC_874|Compound:0599-0792|FD-Score:-3.81|P-value:6.66E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:4.97|P-value:3.97E-7 ID:SGTC_1012|Compound:4112-3522|FD-Score:-3.80|P-value:6.89E-5 ID:SGTC_1024|Compound:4507-0395|FD-Score:3.72|P-value:1.10E-4 ID:SGTC_1178|Compound:0988-0033|FD-Score:3.38|P-value:3.97E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:3.38|P-value:3.95E-4 ID:SGTC_1329|Compound:1447-1697|FD-Score:-3.28|P-value:4.99E-4 ID:SGTC_1342|Compound:1486-1464|FD-Score:3.46|P-value:2.95E-4 ID:SGTC_1612|Compound:st002233|FD-Score:3.28|P-value:5.65E-4 ID:SGTC_1910|Compound:st062352|FD-Score:-3.50|P-value:2.23E-4 ID:SGTC_2016|Compound:5121758|FD-Score:3.60|P-value:1.76E-4 ID:SGTC_2070|Compound:5230947|FD-Score:3.42|P-value:3.42E-4 ID:SGTC_2085|Compound:5307070|FD-Score:3.88|P-value:5.91E-5 ID:SGTC_2119|Compound:5528445|FD-Score:4.03|P-value:3.12E-5 ID:SGTC_2128|Compound:5255449|FD-Score:3.15|P-value:8.85E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.85|P-value:6.50E-5 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:4.21|P-value:1.48E-5 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:-3.57|P-value:1.72E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:3.20|P-value:7.43E-4 ID:SGTC_2589|Compound:lobaric acid|FD-Score:-3.57|P-value:1.72E-4 ID:SGTC_2973|Compound:9094665|FD-Score:-4.37|P-value:6.08E-6 ID:SGTC_2989|Compound:9073649|FD-Score:3.17|P-value:8.26E-4 ID:SGTC_3134|Compound:9127454|FD-Score:4.01|P-value:3.47E-5 ID:SGTC_3147|Compound:9097027|FD-Score:3.94|P-value:4.52E-5 ID:SGTC_3186|Compound:9110464|FD-Score:-3.69|P-value:1.08E-4 ID:SGTC_3347|Compound:9188721|FD-Score:-3.71|P-value:1.01E-4 ID:SGTC_3349|Compound:9149703|FD-Score:3.18|P-value:8.05E-4 ID:SGTC_50|Compound:0929-0078|FD-Score:-3.99|P-value:3.21E-5 ID:SGTC_52|Compound:0329-0193|FD-Score:-4.47|P-value:3.73E-6 ID:SGTC_58|Compound:0957-0393|FD-Score:6.37|P-value:1.20E-10 ID:SGTC_85|Compound:0868-0259|FD-Score:-3.09|P-value:9.68E-4 ID:SGTC_91|Compound:0417-1751|FD-Score:3.32|P-value:4.90E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:4.99|P-value:3.55E-7 ID:SGTC_235|Compound:0330-0087|FD-Score:3.12|P-value:9.88E-4 ID:SGTC_264|Compound:promethazine|FD-Score:-3.28|P-value:5.00E-4 ID:SGTC_271|Compound:rotenone|FD-Score:-3.25|P-value:5.58E-4 ID:SGTC_298|Compound:1391-0729|FD-Score:3.16|P-value:8.53E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:4.38|P-value:6.89E-6 ID:SGTC_679|Compound:0422-0091|FD-Score:3.15|P-value:8.77E-4 ID:SGTC_874|Compound:0599-0792|FD-Score:-3.81|P-value:6.66E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:4.97|P-value:3.97E-7 ID:SGTC_1012|Compound:4112-3522|FD-Score:-3.80|P-value:6.89E-5 ID:SGTC_1024|Compound:4507-0395|FD-Score:3.72|P-value:1.10E-4 ID:SGTC_1178|Compound:0988-0033|FD-Score:3.38|P-value:3.97E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:3.38|P-value:3.95E-4 ID:SGTC_1329|Compound:1447-1697|FD-Score:-3.28|P-value:4.99E-4 ID:SGTC_1342|Compound:1486-1464|FD-Score:3.46|P-value:2.95E-4 ID:SGTC_1612|Compound:st002233|FD-Score:3.28|P-value:5.65E-4 ID:SGTC_1910|Compound:st062352|FD-Score:-3.50|P-value:2.23E-4 ID:SGTC_2016|Compound:5121758|FD-Score:3.60|P-value:1.76E-4 ID:SGTC_2070|Compound:5230947|FD-Score:3.42|P-value:3.42E-4 ID:SGTC_2085|Compound:5307070|FD-Score:3.88|P-value:5.91E-5 ID:SGTC_2119|Compound:5528445|FD-Score:4.03|P-value:3.12E-5 ID:SGTC_2128|Compound:5255449|FD-Score:3.15|P-value:8.85E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.85|P-value:6.50E-5 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:4.21|P-value:1.48E-5 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:-3.57|P-value:1.72E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:3.20|P-value:7.43E-4 ID:SGTC_2589|Compound:lobaric acid|FD-Score:-3.57|P-value:1.72E-4 ID:SGTC_2973|Compound:9094665|FD-Score:-4.37|P-value:6.08E-6 ID:SGTC_2989|Compound:9073649|FD-Score:3.17|P-value:8.26E-4 ID:SGTC_3134|Compound:9127454|FD-Score:4.01|P-value:3.47E-5 ID:SGTC_3147|Compound:9097027|FD-Score:3.94|P-value:4.52E-5 ID:SGTC_3186|Compound:9110464|FD-Score:-3.69|P-value:1.08E-4 ID:SGTC_3347|Compound:9188721|FD-Score:-3.71|P-value:1.01E-4 ID:SGTC_3349|Compound:9149703|FD-Score:3.18|P-value:8.05E-4

Top fitness defect scores for YDR515W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_58 0957-0393 6.37 9.0 µM 1.20E-10 cell wall 188 9.64E-11
2 SGTC_163 1327-0036 4.99 7.5 µM 3.55E-7 64 3.03E-7
3 SGTC_996 3970-0799 4.97 1.8 µM 3.97E-7 56 3.39E-7
4 SGTC_354 2783-3500 4.38 4.9 µM 6.89E-6 cell wall 38 6.04E-6
5 SGTC_2572 astragaloside iv 4.21 100.0 µM 1.48E-5 19 1.30E-5
6 SGTC_2119 5528445 4.03 120.6 µM 3.12E-5 15 2.77E-5
7 SGTC_3134 9127454 4.01 49.5 µM 3.47E-5 10 3.09E-5
8 SGTC_3147 9097027 3.94 49.5 µM 4.52E-5 25 4.02E-5
9 SGTC_2085 5307070 3.88 144.1 µM 5.91E-5 superoxide 29 5.27E-5
10 SGTC_2408 sulbentine 3.85 18.4 µM 6.50E-5 53 5.81E-5
11 SGTC_1024 4507-0395 3.72 26.0 µM 1.10E-4 TSC3-RPN4 33 9.90E-5
12 SGTC_2016 5121758 3.60 140.0 µM 1.76E-4 redox potentiating 70 1.58E-4
13 SGTC_1342 1486-1464 3.46 234.0 µM 2.95E-4 30 2.67E-4
14 SGTC_2070 5230947 3.42 200.0 µM 3.42E-4 26 3.11E-4
15 SGTC_1286 0929-0070 3.38 131.0 µM 3.95E-4 39 3.59E-4
16 SGTC_1178 0988-0033 3.38 191.0 µM 3.97E-4 52 3.60E-4
17 SGTC_91 0417-1751 3.32 58.4 µM 4.90E-4 Golgi 80 4.46E-4
18 SGTC_1612 st002233 3.28 45.4 µM 5.65E-4 38 5.15E-4
19 SGTC_2577 2',4'-dihydroxy-4-methoxychalcone 3.20 15.3 µM 7.43E-4 67 6.80E-4
20 SGTC_3349 9149703 3.18 23.0 µM 8.05E-4 36 7.37E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.192 2.66E-29 YDL189W RBS1 hom Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain
0.180 6.31E-26 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.179 1.46E-25 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.172 1.29E-23 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.160 9.61E-21 YJR013W GPI14 het Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
0.159 1.62E-20 YKL115C_d YKL115C_d hom Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1
0.155 1.50E-19 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.151 1.41E-18 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.148 5.45E-18 YIL160C POT1 hom 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
0.147 1.04E-17 YBR120C CBP6 hom Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.143 6.81E-17 YDL099W BUG1 hom Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.143 9.80E-17 YOR026W BUB3 hom Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p
0.139 7.16E-16 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.139 7.69E-16 YCL005W LDB16 hom Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria
0.138 1.19E-15 YGL222C EDC1 hom RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress