YDR517W / GRH1

Acetylated cis-Golgi protein, involved in ER to Golgi transport; homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR517W / GRH1

Click on Significant Values for Screen Details ID:SGTC_27|Compound:0485-0001|FD-Score:3.08|P-value:3.35E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:-3.44|P-value:7.28E-5 ID:SGTC_338|Compound:4055-0136|FD-Score:3.10|P-value:3.14E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:-3.42|P-value:7.95E-5 ID:SGTC_424|Compound:splitomicin|FD-Score:3.08|P-value:3.37E-4 ID:SGTC_520|Compound:3584-0023|FD-Score:2.90|P-value:6.85E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:3.01|P-value:4.47E-4 ID:SGTC_710|Compound:3448-4187|FD-Score:-2.96|P-value:5.44E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:-3.51|P-value:5.28E-5 ID:SGTC_1016|Compound:4226-1401|FD-Score:-3.83|P-value:1.18E-5 ID:SGTC_1248|Compound:0469-0687|FD-Score:4.11|P-value:2.81E-6 ID:SGTC_1256|Compound:0570-0116|FD-Score:-3.39|P-value:9.17E-5 ID:SGTC_1299|Compound:1079-1439|FD-Score:-3.21|P-value:1.98E-4 ID:SGTC_1363|Compound:1636-0063|FD-Score:-2.81|P-value:9.46E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-6.38|P-value:9.29E-13 ID:SGTC_1463|Compound:k081-0032|FD-Score:-5.40|P-value:1.20E-9 ID:SGTC_1736|Compound:st036774|FD-Score:2.91|P-value:6.46E-4 ID:SGTC_1756|Compound:st041558|FD-Score:3.30|P-value:1.34E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-3.57|P-value:4.01E-5 ID:SGTC_1906|Compound:st060206|FD-Score:2.99|P-value:4.81E-4 ID:SGTC_1915|Compound:st067105|FD-Score:-3.31|P-value:1.28E-4 ID:SGTC_1931|Compound:st074438|FD-Score:-3.55|P-value:4.51E-5 ID:SGTC_2091|Compound:4-phenylthiadiazole|FD-Score:-2.81|P-value:9.39E-4 ID:SGTC_2101|Compound:5377231|FD-Score:-3.17|P-value:2.28E-4 ID:SGTC_2131|Compound:toluylene red|FD-Score:-3.10|P-value:3.12E-4 ID:SGTC_2305|Compound:7443522|FD-Score:3.65|P-value:2.84E-5 ID:SGTC_2307|Compound:7538926|FD-Score:-2.83|P-value:8.91E-4 ID:SGTC_2316|Compound:5844804|FD-Score:3.22|P-value:1.91E-4 ID:SGTC_2344|Compound:7990960|FD-Score:3.38|P-value:9.57E-5 ID:SGTC_2351|Compound:9026858|FD-Score:-3.98|P-value:5.62E-6 ID:SGTC_2372|Compound:9071633|FD-Score:4.35|P-value:8.01E-7 ID:SGTC_2374|Compound:9071832|FD-Score:3.03|P-value:4.15E-4 ID:SGTC_2384|Compound:9036245|FD-Score:3.22|P-value:1.88E-4 ID:SGTC_2387|Compound:9077560|FD-Score:-3.62|P-value:3.23E-5 ID:SGTC_2438|Compound:5259590|FD-Score:2.80|P-value:9.97E-4 ID:SGTC_2452|Compound:5863136|FD-Score:-2.98|P-value:4.98E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:3.93|P-value:7.10E-6 ID:SGTC_2600|Compound:tangeritin|FD-Score:-3.77|P-value:1.60E-5 ID:SGTC_2601|Compound:securinine|FD-Score:-3.27|P-value:1.51E-4 ID:SGTC_2602|Compound:kawain|FD-Score:2.82|P-value:9.43E-4 ID:SGTC_2898|Compound:9056270|FD-Score:3.62|P-value:3.17E-5 ID:SGTC_3171|Compound:9103192|FD-Score:-2.86|P-value:8.05E-4 ID:SGTC_27|Compound:0485-0001|FD-Score:3.08|P-value:3.35E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:-3.44|P-value:7.28E-5 ID:SGTC_338|Compound:4055-0136|FD-Score:3.10|P-value:3.14E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:-3.42|P-value:7.95E-5 ID:SGTC_424|Compound:splitomicin|FD-Score:3.08|P-value:3.37E-4 ID:SGTC_520|Compound:3584-0023|FD-Score:2.90|P-value:6.85E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:3.01|P-value:4.47E-4 ID:SGTC_710|Compound:3448-4187|FD-Score:-2.96|P-value:5.44E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:-3.51|P-value:5.28E-5 ID:SGTC_1016|Compound:4226-1401|FD-Score:-3.83|P-value:1.18E-5 ID:SGTC_1248|Compound:0469-0687|FD-Score:4.11|P-value:2.81E-6 ID:SGTC_1256|Compound:0570-0116|FD-Score:-3.39|P-value:9.17E-5 ID:SGTC_1299|Compound:1079-1439|FD-Score:-3.21|P-value:1.98E-4 ID:SGTC_1363|Compound:1636-0063|FD-Score:-2.81|P-value:9.46E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-6.38|P-value:9.29E-13 ID:SGTC_1463|Compound:k081-0032|FD-Score:-5.40|P-value:1.20E-9 ID:SGTC_1736|Compound:st036774|FD-Score:2.91|P-value:6.46E-4 ID:SGTC_1756|Compound:st041558|FD-Score:3.30|P-value:1.34E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-3.57|P-value:4.01E-5 ID:SGTC_1906|Compound:st060206|FD-Score:2.99|P-value:4.81E-4 ID:SGTC_1915|Compound:st067105|FD-Score:-3.31|P-value:1.28E-4 ID:SGTC_1931|Compound:st074438|FD-Score:-3.55|P-value:4.51E-5 ID:SGTC_2091|Compound:4-phenylthiadiazole|FD-Score:-2.81|P-value:9.39E-4 ID:SGTC_2101|Compound:5377231|FD-Score:-3.17|P-value:2.28E-4 ID:SGTC_2131|Compound:toluylene red|FD-Score:-3.10|P-value:3.12E-4 ID:SGTC_2305|Compound:7443522|FD-Score:3.65|P-value:2.84E-5 ID:SGTC_2307|Compound:7538926|FD-Score:-2.83|P-value:8.91E-4 ID:SGTC_2316|Compound:5844804|FD-Score:3.22|P-value:1.91E-4 ID:SGTC_2344|Compound:7990960|FD-Score:3.38|P-value:9.57E-5 ID:SGTC_2351|Compound:9026858|FD-Score:-3.98|P-value:5.62E-6 ID:SGTC_2372|Compound:9071633|FD-Score:4.35|P-value:8.01E-7 ID:SGTC_2374|Compound:9071832|FD-Score:3.03|P-value:4.15E-4 ID:SGTC_2384|Compound:9036245|FD-Score:3.22|P-value:1.88E-4 ID:SGTC_2387|Compound:9077560|FD-Score:-3.62|P-value:3.23E-5 ID:SGTC_2438|Compound:5259590|FD-Score:2.80|P-value:9.97E-4 ID:SGTC_2452|Compound:5863136|FD-Score:-2.98|P-value:4.98E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:3.93|P-value:7.10E-6 ID:SGTC_2600|Compound:tangeritin|FD-Score:-3.77|P-value:1.60E-5 ID:SGTC_2601|Compound:securinine|FD-Score:-3.27|P-value:1.51E-4 ID:SGTC_2602|Compound:kawain|FD-Score:2.82|P-value:9.43E-4 ID:SGTC_2898|Compound:9056270|FD-Score:3.62|P-value:3.17E-5 ID:SGTC_3171|Compound:9103192|FD-Score:-2.86|P-value:8.05E-4

Top fitness defect scores for YDR517W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2372 9071633 4.35 200.0 µM 8.01E-7 9 6.92E-6
2 SGTC_1248 0469-0687 4.11 58.6 µM 2.81E-6 24 1.95E-5
3 SGTC_2514 salinomycin 3.93 42.7 µM 7.10E-6 48 4.21E-5
4 SGTC_2305 7443522 3.65 69.2 µM 2.84E-5 21 1.33E-4
5 SGTC_2898 9056270 3.62 58.4 µM 3.17E-5 8 1.45E-4
6 SGTC_2344 7990960 3.38 77.0 µM 9.57E-5 44 3.64E-4
7 SGTC_1756 st041558 3.30 35.5 µM 1.34E-4 21 4.82E-4
8 SGTC_2384 9036245 3.22 200.0 µM 1.88E-4 56 6.36E-4
9 SGTC_2316 5844804 3.22 197.4 µM 1.91E-4 74 6.44E-4
10 SGTC_338 4055-0136 3.10 114.0 µM 3.14E-4 36 9.77E-4
11 SGTC_27 0485-0001 3.08 32.6 µM 3.35E-4 RPP1 & pyrimidine depletion 18 0.00103
12 SGTC_424 splitomicin 3.08 100.0 mM 3.37E-4 63 0.00103
13 SGTC_2374 9071832 3.03 200.0 µM 4.15E-4 endomembrane recycling 33 0.00123
14 SGTC_696 1560-0013 3.01 611.0 µM 4.47E-4 63 0.00131
15 SGTC_1906 st060206 2.99 94.7 µM 4.81E-4 62 0.00139
16 SGTC_1736 st036774 2.91 51.0 µM 6.46E-4 83 0.00178
17 SGTC_520 3584-0023 2.90 682.0 µM 6.85E-4 76 0.00187
18 SGTC_2602 kawain 2.82 100.0 µM 9.43E-4 93 0.00244
19 SGTC_2438 5259590 2.80 200.0 µM 9.97E-4 63 0.00255
20 SGTC_1452 4194-0072 2.79 190.0 µM 0.00102 73 0.00261

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.325 3.09E-83 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.308 1.45E-74 YGR136W LSB1 hom Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress
0.293 2.78E-67 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.291 2.82E-66 YDR521W_d YDR521W_d hom Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.277 3.78E-60 YNL166C BNI5 hom Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.275 3.18E-59 YFL014W HSP12 hom Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension
0.268 3.68E-56 YPR180W AOS1 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.255 4.38E-51 YOR152C_p YOR152C_p hom Putative protein of unknown function; YOR152C is not an essential gene
0.252 1.22E-49 YGR077C PEX8 hom Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
0.244 8.27E-47 YDL157C_p YDL157C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.236 1.38E-43 YMR106C YKU80 hom Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair
0.232 3.57E-42 YDL176W YDL176W hom Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene
0.230 1.16E-41 YBL017C PEP1 hom Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments
0.227 1.98E-40 YDR312W SSF2 hom Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication
0.227 1.84E-40 YDR497C ITR1 hom Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress