YDR520C_p / URC2

Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR520C / URC2

Click on Significant Values for Screen Details ID:SGTC_397|Compound:arbidol|FD-Score:-3.41|P-value:7.36E-4 ID:SGTC_401|Compound:neomycin|FD-Score:3.61|P-value:5.73E-4 ID:SGTC_407|Compound:eburnamonine|FD-Score:3.91|P-value:2.04E-4 ID:SGTC_427|Compound:1354-0053|FD-Score:4.33|P-value:4.52E-5 ID:SGTC_572|Compound:0960-0087|FD-Score:4.75|P-value:8.57E-6 ID:SGTC_574|Compound:1315-0397|FD-Score:6.20|P-value:9.06E-9 ID:SGTC_785|Compound:1270-0111|FD-Score:3.57|P-value:6.52E-4 ID:SGTC_1063|Compound:disulfiram|FD-Score:4.79|P-value:7.15E-6 ID:SGTC_1078|Compound:idebenone|FD-Score:3.51|P-value:7.80E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:5.02|P-value:2.71E-6 ID:SGTC_1612|Compound:st002233|FD-Score:5.05|P-value:2.41E-6 ID:SGTC_1717|Compound:st033143|FD-Score:-3.88|P-value:1.52E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:5.05|P-value:2.33E-6 ID:SGTC_1724|Compound:st037281|FD-Score:4.54|P-value:1.98E-5 ID:SGTC_1736|Compound:st036774|FD-Score:-3.90|P-value:1.42E-4 ID:SGTC_1793|Compound:st048256|FD-Score:-4.77|P-value:4.79E-6 ID:SGTC_1799|Compound:st045822|FD-Score:-3.54|P-value:4.87E-4 ID:SGTC_1828|Compound:st055811|FD-Score:-3.55|P-value:4.71E-4 ID:SGTC_2284|Compound:7945075|FD-Score:-5.07|P-value:1.30E-6 ID:SGTC_2291|Compound:7917025|FD-Score:3.92|P-value:2.03E-4 ID:SGTC_2332|Compound:7982454|FD-Score:4.29|P-value:5.18E-5 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:-4.80|P-value:4.36E-6 ID:SGTC_2858|Compound:9025781|FD-Score:3.62|P-value:5.43E-4 ID:SGTC_2864|Compound:9034755|FD-Score:3.74|P-value:3.75E-4 ID:SGTC_2898|Compound:9056270|FD-Score:-3.76|P-value:2.37E-4 ID:SGTC_397|Compound:arbidol|FD-Score:-3.41|P-value:7.36E-4 ID:SGTC_401|Compound:neomycin|FD-Score:3.61|P-value:5.73E-4 ID:SGTC_407|Compound:eburnamonine|FD-Score:3.91|P-value:2.04E-4 ID:SGTC_427|Compound:1354-0053|FD-Score:4.33|P-value:4.52E-5 ID:SGTC_572|Compound:0960-0087|FD-Score:4.75|P-value:8.57E-6 ID:SGTC_574|Compound:1315-0397|FD-Score:6.20|P-value:9.06E-9 ID:SGTC_785|Compound:1270-0111|FD-Score:3.57|P-value:6.52E-4 ID:SGTC_1063|Compound:disulfiram|FD-Score:4.79|P-value:7.15E-6 ID:SGTC_1078|Compound:idebenone|FD-Score:3.51|P-value:7.80E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:5.02|P-value:2.71E-6 ID:SGTC_1612|Compound:st002233|FD-Score:5.05|P-value:2.41E-6 ID:SGTC_1717|Compound:st033143|FD-Score:-3.88|P-value:1.52E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:5.05|P-value:2.33E-6 ID:SGTC_1724|Compound:st037281|FD-Score:4.54|P-value:1.98E-5 ID:SGTC_1736|Compound:st036774|FD-Score:-3.90|P-value:1.42E-4 ID:SGTC_1793|Compound:st048256|FD-Score:-4.77|P-value:4.79E-6 ID:SGTC_1799|Compound:st045822|FD-Score:-3.54|P-value:4.87E-4 ID:SGTC_1828|Compound:st055811|FD-Score:-3.55|P-value:4.71E-4 ID:SGTC_2284|Compound:7945075|FD-Score:-5.07|P-value:1.30E-6 ID:SGTC_2291|Compound:7917025|FD-Score:3.92|P-value:2.03E-4 ID:SGTC_2332|Compound:7982454|FD-Score:4.29|P-value:5.18E-5 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:-4.80|P-value:4.36E-6 ID:SGTC_2858|Compound:9025781|FD-Score:3.62|P-value:5.43E-4 ID:SGTC_2864|Compound:9034755|FD-Score:3.74|P-value:3.75E-4 ID:SGTC_2898|Compound:9056270|FD-Score:-3.76|P-value:2.37E-4

Top fitness defect scores for YDR520C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_574 1315-0397 6.20 40.0 µM 9.06E-9 5 2.83E-10
2 SGTC_1718 roemerine 5.05 11.9 µM 2.33E-6 redox potentiating 21 2.16E-7
3 SGTC_1612 st002233 5.05 45.4 µM 2.41E-6 5 2.24E-7
4 SGTC_1110 3-hydroxy-3'-methoxyflavone 5.02 1.2 µM 2.71E-6 iron homeostasis 24 2.58E-7
5 SGTC_1063 disulfiram 4.79 1.8 µM 7.15E-6 9 8.25E-7
6 SGTC_572 0960-0087 4.75 17.7 µM 8.57E-6 12 1.02E-6
7 SGTC_1724 st037281 4.54 8.3 µM 1.98E-5 29 2.78E-6
8 SGTC_427 1354-0053 4.33 47.3 µM 4.52E-5 10 7.48E-6
9 SGTC_2332 7982454 4.29 138.9 µM 5.18E-5 37 8.80E-6
10 SGTC_2291 7917025 3.92 104.3 µM 2.03E-4 iron homeostasis 47 4.51E-5
11 SGTC_407 eburnamonine 3.91 100.0 µM 2.04E-4 11 4.54E-5
12 SGTC_2864 9034755 3.74 47.6 µM 3.75E-4 11 9.37E-5
13 SGTC_2858 9025781 3.62 9.0 µM 5.43E-4 sphingolipid biosynthesis & PDR1 114 1.46E-4
14 SGTC_401 neomycin 3.61 50.0 µM 5.73E-4 15 1.56E-4
15 SGTC_785 1270-0111 3.57 65.3 µM 6.52E-4 44 1.82E-4
16 SGTC_1078 idebenone 3.51 36.1 µM 7.80E-4 Golgi 58 2.25E-4
17 SGTC_1310 1188-0297 3.38 994.0 nM 0.00115 azole & statin 48 3.59E-4
18 SGTC_2782 7736369 3.38 71.4 µM 0.00117 67 3.64E-4
19 SGTC_1130 3454-2858 3.38 15.9 µM 0.00117 NEO1 117 3.66E-4
20 SGTC_2869 9037858 3.37 32.5 µM 0.00119 28 3.71E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.157 4.91E-20 YGR144W THI4 hom Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents
0.150 2.92E-18 YDR270W CCC2 hom Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism
0.141 2.15E-16 YMR058W FET3 hom Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress
0.135 3.81E-15 YLR329W REC102 hom Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination
0.129 6.80E-14 YDR202C RAV2 hom Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme
0.111 1.15E-10 YER073W ALD5 hom Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed
0.106 8.28E-10 YDR376W ARH1 het Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability
0.102 3.56E-9 YOR282W_d YOR282W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
0.096 2.59E-8 YGR168C_p YGR168C_p hom Putative protein of unknown function; YGR168C is not an essential gene
0.090 1.99E-7 YML096W_p YML096W_p hom Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10
0.089 2.17E-7 YGL028C SCW11 hom Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p
0.087 5.04E-7 YDL178W DLD2 hom D-lactate dehydrogenase, located in the mitochondrial matrix
0.084 1.10E-6 YNL259C ATX1 hom Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake
0.082 1.73E-6 YML009C MRPL39 hom Mitochondrial ribosomal protein of the large subunit
0.082 1.79E-6 YHR042W NCP1 het NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p