YDR521W_d

Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR521W / YDR521W

Click on Significant Values for Screen Details ID:SGTC_74|Compound:000s-0487|FD-Score:-2.98|P-value:5.56E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:-3.21|P-value:2.21E-4 ID:SGTC_381|Compound:3461-2219|FD-Score:4.77|P-value:7.37E-8 ID:SGTC_407|Compound:eburnamonine|FD-Score:-3.07|P-value:3.92E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:-3.46|P-value:7.53E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:2.98|P-value:4.93E-4 ID:SGTC_1100|Compound:amiodarone|FD-Score:3.31|P-value:1.31E-4 ID:SGTC_1142|Compound:3253-1408|FD-Score:3.12|P-value:2.91E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:3.05|P-value:3.84E-4 ID:SGTC_1461|Compound:k072-0203|FD-Score:-3.81|P-value:1.53E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-10.10|P-value:1.26E-28 ID:SGTC_1463|Compound:k081-0032|FD-Score:-9.85|P-value:1.53E-27 ID:SGTC_1564|Compound:D-Arginine|FD-Score:-3.72|P-value:2.28E-5 ID:SGTC_1913|Compound:st060213|FD-Score:2.85|P-value:8.34E-4 ID:SGTC_1973|Compound:st070275|FD-Score:3.60|P-value:3.50E-5 ID:SGTC_1975|Compound:st070967|FD-Score:3.14|P-value:2.68E-4 ID:SGTC_1976|Compound:st070609|FD-Score:3.20|P-value:2.08E-4 ID:SGTC_2014|Compound:4013930|FD-Score:4.49|P-value:3.63E-7 ID:SGTC_2024|Compound:5144227|FD-Score:2.96|P-value:5.45E-4 ID:SGTC_2029|Compound:5175081|FD-Score:-2.83|P-value:9.99E-4 ID:SGTC_2126|Compound:5251174|FD-Score:2.93|P-value:6.07E-4 ID:SGTC_2354|Compound:9060492|FD-Score:3.72|P-value:2.06E-5 ID:SGTC_2438|Compound:5259590|FD-Score:3.09|P-value:3.27E-4 ID:SGTC_2452|Compound:5863136|FD-Score:-4.23|P-value:1.83E-6 ID:SGTC_2453|Compound:5731132|FD-Score:3.31|P-value:1.28E-4 ID:SGTC_2458|Compound:5808907|FD-Score:4.04|P-value:4.17E-6 ID:SGTC_2471|Compound:5466956|FD-Score:2.83|P-value:8.87E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:3.64|P-value:3.00E-5 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:3.27|P-value:1.52E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:-3.02|P-value:4.83E-4 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:-4.11|P-value:3.33E-6 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:-4.03|P-value:4.93E-6 ID:SGTC_2585|Compound:alliin|FD-Score:3.01|P-value:4.46E-4 ID:SGTC_2586|Compound:curcumin|FD-Score:2.93|P-value:6.05E-4 ID:SGTC_2589|Compound:lobaric acid|FD-Score:-3.18|P-value:2.52E-4 ID:SGTC_2725|Compound:haloperidol|FD-Score:-3.39|P-value:1.04E-4 ID:SGTC_2812|Compound:7970281|FD-Score:2.81|P-value:9.59E-4 ID:SGTC_2853|Compound:9015042|FD-Score:-3.25|P-value:1.88E-4 ID:SGTC_2898|Compound:9056270|FD-Score:3.23|P-value:1.78E-4 ID:SGTC_2899|Compound:9053889|FD-Score:3.46|P-value:6.65E-5 ID:SGTC_74|Compound:000s-0487|FD-Score:-2.98|P-value:5.56E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:-3.21|P-value:2.21E-4 ID:SGTC_381|Compound:3461-2219|FD-Score:4.77|P-value:7.37E-8 ID:SGTC_407|Compound:eburnamonine|FD-Score:-3.07|P-value:3.92E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:-3.46|P-value:7.53E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:2.98|P-value:4.93E-4 ID:SGTC_1100|Compound:amiodarone|FD-Score:3.31|P-value:1.31E-4 ID:SGTC_1142|Compound:3253-1408|FD-Score:3.12|P-value:2.91E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:3.05|P-value:3.84E-4 ID:SGTC_1461|Compound:k072-0203|FD-Score:-3.81|P-value:1.53E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-10.10|P-value:1.26E-28 ID:SGTC_1463|Compound:k081-0032|FD-Score:-9.85|P-value:1.53E-27 ID:SGTC_1564|Compound:D-Arginine|FD-Score:-3.72|P-value:2.28E-5 ID:SGTC_1913|Compound:st060213|FD-Score:2.85|P-value:8.34E-4 ID:SGTC_1973|Compound:st070275|FD-Score:3.60|P-value:3.50E-5 ID:SGTC_1975|Compound:st070967|FD-Score:3.14|P-value:2.68E-4 ID:SGTC_1976|Compound:st070609|FD-Score:3.20|P-value:2.08E-4 ID:SGTC_2014|Compound:4013930|FD-Score:4.49|P-value:3.63E-7 ID:SGTC_2024|Compound:5144227|FD-Score:2.96|P-value:5.45E-4 ID:SGTC_2029|Compound:5175081|FD-Score:-2.83|P-value:9.99E-4 ID:SGTC_2126|Compound:5251174|FD-Score:2.93|P-value:6.07E-4 ID:SGTC_2354|Compound:9060492|FD-Score:3.72|P-value:2.06E-5 ID:SGTC_2438|Compound:5259590|FD-Score:3.09|P-value:3.27E-4 ID:SGTC_2452|Compound:5863136|FD-Score:-4.23|P-value:1.83E-6 ID:SGTC_2453|Compound:5731132|FD-Score:3.31|P-value:1.28E-4 ID:SGTC_2458|Compound:5808907|FD-Score:4.04|P-value:4.17E-6 ID:SGTC_2471|Compound:5466956|FD-Score:2.83|P-value:8.87E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:3.64|P-value:3.00E-5 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:3.27|P-value:1.52E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:-3.02|P-value:4.83E-4 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:-4.11|P-value:3.33E-6 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:-4.03|P-value:4.93E-6 ID:SGTC_2585|Compound:alliin|FD-Score:3.01|P-value:4.46E-4 ID:SGTC_2586|Compound:curcumin|FD-Score:2.93|P-value:6.05E-4 ID:SGTC_2589|Compound:lobaric acid|FD-Score:-3.18|P-value:2.52E-4 ID:SGTC_2725|Compound:haloperidol|FD-Score:-3.39|P-value:1.04E-4 ID:SGTC_2812|Compound:7970281|FD-Score:2.81|P-value:9.59E-4 ID:SGTC_2853|Compound:9015042|FD-Score:-3.25|P-value:1.88E-4 ID:SGTC_2898|Compound:9056270|FD-Score:3.23|P-value:1.78E-4 ID:SGTC_2899|Compound:9053889|FD-Score:3.46|P-value:6.65E-5

Top fitness defect scores for YDR521W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_381 3461-2219 4.77 42.7 µM 7.37E-8 copper-dependent oxidative stress 10 9.36E-7
2 SGTC_2014 4013930 4.49 200.0 µM 3.63E-7 15 3.52E-6
3 SGTC_2458 5808907 4.04 16.4 µM 4.17E-6 4 2.68E-5
4 SGTC_2354 9060492 3.72 162.0 µM 2.06E-5 22 1.01E-4
5 SGTC_2533 agelasine 3.64 13.7 µM 3.00E-5 ERG2 58 1.39E-4
6 SGTC_1973 st070275 3.60 50.2 µM 3.50E-5 21 1.57E-4
7 SGTC_2899 9053889 3.46 58.4 µM 6.65E-5 20 2.68E-4
8 SGTC_2453 5731132 3.31 22.2 µM 1.28E-4 44 4.64E-4
9 SGTC_1100 amiodarone 3.31 5.1 µM 1.31E-4 35 4.73E-4
10 SGTC_2536 dehydroabietamide 3.27 32.0 µM 1.52E-4 67 5.34E-4
11 SGTC_2898 9056270 3.23 58.4 µM 1.78E-4 13 6.09E-4
12 SGTC_1976 st070609 3.20 63.5 µM 2.08E-4 33 6.96E-4
13 SGTC_1975 st070967 3.14 30.8 µM 2.68E-4 73 8.59E-4
14 SGTC_1142 3253-1408 3.12 9.8 µM 2.91E-4 32 9.20E-4
15 SGTC_2438 5259590 3.09 200.0 µM 3.27E-4 45 0.00101
16 SGTC_1379 2144-0755 3.05 36.5 µM 3.84E-4 56 0.00116
17 SGTC_2585 alliin 3.01 100.0 µM 4.46E-4 77 0.00131
18 SGTC_996 3970-0799 2.98 1.8 µM 4.93E-4 155 0.00143
19 SGTC_2024 5144227 2.96 134.0 µM 5.45E-4 90 0.00155
20 SGTC_2586 curcumin 2.93 80.0 µM 6.05E-4 iron homeostasis 121 0.00170

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.486 4.48E-198 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.461 1.88E-176 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.433 1.28E-152 YPR180W AOS1 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.433 1.33E-152 YNL166C BNI5 hom Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.398 1.06E-127 YDR169C STB3 hom Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress
0.387 3.58E-120 YFL014W HSP12 hom Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension
0.375 4.74E-112 YDL176W YDL176W hom Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene
0.362 3.91E-104 YGR077C PEX8 hom Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
0.356 1.34E-100 YDL157C_p YDL157C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.342 1.63E-92 YOR152C_p YOR152C_p hom Putative protein of unknown function; YOR152C is not an essential gene
0.338 3.86E-90 YCL026C-A FRM2 hom Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin
0.331 1.76E-86 YLL054C_p YLL054C_p hom Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene
0.327 3.42E-84 YJR146W_p YJR146W_p hom Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2
0.323 7.16E-82 YDR312W SSF2 hom Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication
0.321 6.73E-81 YGR136W LSB1 hom Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress