HIPHOP chemogenomics database

YEL032W / MCM3

Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex

Zygosity: Heterozygous strain

fixedexpanded
Profile for YEL032W / MCM3

Click on Significant Values for Screen Details

Top fitness defect scores for YEL032W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_264	promethazine	9.31	12.5 mM	1.22E-17	DNA intercalators	6	6.66E-21	0.06
2	SGTC_1926	methyl fluorone black	7.51	53.6 µM	4.17E-12	DNA intercalators	5	3.03E-14	0.19
3	SGTC_145	3737-0113	6.68	38.4 µM	5.93E-10	DNA intercalators	11	1.17E-11	0.08
4	SGTC_1468	k284-1086	6.46	134.0 µM	2.11E-9	DNA intercalators	3	5.40E-11	0.34
5	SGTC_2424	5537928	5.97	59.2 µM	2.83E-8		1	1.22E-9	0.68
6	SGTC_1515	3460-0031	5.96	42.1 µM	2.94E-8	DNA intercalators	3	1.27E-9	1.57
7	SGTC_144	4161-2749	5.84	112.4 µM	5.47E-8	DNA intercalators	4	2.69E-9	0.46
8	SGTC_2592	piperlongumine	5.38	83.3 µM	4.87E-7	TSC3-RPN4	2	3.71E-8	0.86
9	SGTC_1391	3442-0225	4.94	151.0 µM	3.42E-6		2	3.84E-7	0.98
10	SGTC_2467	5474540	4.71	200.0 µM	8.98E-6		2	1.22E-6	0.23
11	SGTC_1290	0986-0249	4.68	10.2 µM	1.01E-5	DNA intercalators	5	1.41E-6	0.04
12	SGTC_1494	4358-1428	4.54	177.0 µM	1.81E-5		6	2.83E-6	0.92
13	SGTC_3211	9128781	4.52	49.5 µM	1.94E-5		11	3.06E-6	0.24
14	SGTC_999	4022-0066	4.34	22.7 µM	3.99E-5	Golgi	9	7.26E-6	0.15
15	SGTC_1519	esterone	4.32	74.0 µM	4.17E-5	DNA intercalators	5	7.65E-6	0.39
16	SGTC_3040	9091894	4.27	49.5 µM	5.13E-5	DNA intercalators	9	9.82E-6	0.38
17	SGTC_9	0092-0002	4.23	10.4 µM	5.83E-5		12	1.14E-5	0.23
18	SGTC_1763	st044482	4.15	94.5 µM	8.04E-5	copper-dependent oxidative stress	3	1.68E-5	0.42
19	SGTC_1417	3966-0327	4.10	90.4 µM	9.72E-5		2	2.11E-5	0.63
20	SGTC_3147	9097027	4.05	49.5 µM	1.15E-4		5	2.57E-5	0.14

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.434	5.15E-154	YMR168C	CEP3	het	Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.410	1.26E-135	YMR166C_p	YMR166C_p	hom	Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
0.403	6.77E-131	YGL118C_d	YGL118C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.378	5.03E-114	YMR289W	ABZ2	hom	Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
0.363	5.83E-105	YHL039W	EFM1	hom	Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.347	2.84E-95	YDL223C	HBT1	hom	Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis
0.336	1.96E-89	YOR133W	EFT1	hom	Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication
0.336	7.21E-89	YJL025W	RRN7	het	Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
0.310	1.49E-75	YGR065C	VHT1	het	High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin
0.282	2.86E-62	YMR262W_p	YMR262W_p	hom	Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene
0.254	2.20E-50	YLR133W	CKI1	hom	Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication
0.249	1.92E-48	YGL041C_d	YGL041C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.244	1.10E-46	YGL200C	EMP24	hom	Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles
0.233	1.08E-42	YPL157W	TGS1	hom	Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs
0.232	2.45E-42	YDL102W	POL3	het	Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)