YEL047C / FRD1

Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YEL047C / FRD1

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:5.31|P-value:3.46E-8 ID:SGTC_44|Compound:0929-0055|FD-Score:-3.18|P-value:6.40E-4 ID:SGTC_98|Compound:0074-0023|FD-Score:3.91|P-value:3.57E-5 ID:SGTC_359|Compound:0081-0058|FD-Score:-3.82|P-value:5.25E-5 ID:SGTC_379|Compound:3970-0972|FD-Score:3.08|P-value:8.96E-4 ID:SGTC_380|Compound:3970-0968|FD-Score:4.15|P-value:1.27E-5 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:3.26|P-value:4.77E-4 ID:SGTC_401|Compound:neomycin|FD-Score:3.84|P-value:4.97E-5 ID:SGTC_403|Compound:arphamenine a|FD-Score:-3.40|P-value:2.86E-4 ID:SGTC_418|Compound:lividomycin a|FD-Score:-3.27|P-value:4.49E-4 ID:SGTC_795|Compound:0685-0265|FD-Score:3.09|P-value:8.51E-4 ID:SGTC_840|Compound:0245-0196|FD-Score:5.02|P-value:1.77E-7 ID:SGTC_852|Compound:0418-0143|FD-Score:3.32|P-value:3.77E-4 ID:SGTC_853|Compound:0419-0306|FD-Score:3.54|P-value:1.62E-4 ID:SGTC_965|Compound:1291-5240|FD-Score:4.23|P-value:8.76E-6 ID:SGTC_1118|Compound:chelidonine|FD-Score:3.37|P-value:3.13E-4 ID:SGTC_1233|Compound:0325-0408|FD-Score:3.36|P-value:3.25E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:3.34|P-value:3.49E-4 ID:SGTC_1451|Compound:4152-0210|FD-Score:3.37|P-value:3.10E-4 ID:SGTC_1452|Compound:4194-0072|FD-Score:3.68|P-value:9.48E-5 ID:SGTC_1492|Compound:4158-0558|FD-Score:3.45|P-value:2.30E-4 ID:SGTC_1605|Compound:st000082|FD-Score:3.27|P-value:4.59E-4 ID:SGTC_1629|Compound:st005085|FD-Score:-5.99|P-value:6.24E-10 ID:SGTC_1635|Compound:st007035|FD-Score:-4.03|P-value:2.19E-5 ID:SGTC_1637|Compound:st007924|FD-Score:-12.70|P-value:2.45E-38 ID:SGTC_1731|Compound:st035964|FD-Score:3.22|P-value:5.40E-4 ID:SGTC_1866|Compound:st057638|FD-Score:3.87|P-value:4.31E-5 ID:SGTC_2064|Compound:5220758|FD-Score:3.84|P-value:4.94E-5 ID:SGTC_2394|Compound:5221648|FD-Score:4.58|P-value:1.68E-6 ID:SGTC_2399|Compound:5884133|FD-Score:-3.08|P-value:8.99E-4 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:-3.43|P-value:2.49E-4 ID:SGTC_2446|Compound:5162541|FD-Score:3.17|P-value:6.55E-4 ID:SGTC_2483|Compound:5729050|FD-Score:-3.27|P-value:4.50E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:4.01|P-value:2.40E-5 ID:SGTC_2706|Compound:st078239|FD-Score:3.35|P-value:3.32E-4 ID:SGTC_2715|Compound:st079500|FD-Score:3.08|P-value:8.82E-4 ID:SGTC_2783|Compound:7738165|FD-Score:-3.32|P-value:3.80E-4 ID:SGTC_2784|Compound:7567673|FD-Score:-3.61|P-value:1.24E-4 ID:SGTC_2788|Compound:5282734|FD-Score:4.67|P-value:1.07E-6 ID:SGTC_2835|Compound:9006588|FD-Score:-4.61|P-value:1.48E-6 ID:SGTC_3027|Compound:9089541|FD-Score:-3.40|P-value:2.80E-4 ID:SGTC_3031|Compound:9088762|FD-Score:-4.09|P-value:1.67E-5 ID:SGTC_3057|Compound:9095946|FD-Score:3.61|P-value:1.23E-4 ID:SGTC_3221|Compound:9128209|FD-Score:4.23|P-value:8.74E-6 ID:SGTC_3260|Compound:9137109|FD-Score:4.26|P-value:7.64E-6 ID:SGTC_3273|Compound:9137859|FD-Score:3.89|P-value:3.99E-5 ID:SGTC_3282|Compound:9108498|FD-Score:3.49|P-value:2.00E-4 ID:SGTC_3306|Compound:9127676|FD-Score:3.10|P-value:8.33E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:5.31|P-value:3.46E-8 ID:SGTC_44|Compound:0929-0055|FD-Score:-3.18|P-value:6.40E-4 ID:SGTC_98|Compound:0074-0023|FD-Score:3.91|P-value:3.57E-5 ID:SGTC_359|Compound:0081-0058|FD-Score:-3.82|P-value:5.25E-5 ID:SGTC_379|Compound:3970-0972|FD-Score:3.08|P-value:8.96E-4 ID:SGTC_380|Compound:3970-0968|FD-Score:4.15|P-value:1.27E-5 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:3.26|P-value:4.77E-4 ID:SGTC_401|Compound:neomycin|FD-Score:3.84|P-value:4.97E-5 ID:SGTC_403|Compound:arphamenine a|FD-Score:-3.40|P-value:2.86E-4 ID:SGTC_418|Compound:lividomycin a|FD-Score:-3.27|P-value:4.49E-4 ID:SGTC_795|Compound:0685-0265|FD-Score:3.09|P-value:8.51E-4 ID:SGTC_840|Compound:0245-0196|FD-Score:5.02|P-value:1.77E-7 ID:SGTC_852|Compound:0418-0143|FD-Score:3.32|P-value:3.77E-4 ID:SGTC_853|Compound:0419-0306|FD-Score:3.54|P-value:1.62E-4 ID:SGTC_965|Compound:1291-5240|FD-Score:4.23|P-value:8.76E-6 ID:SGTC_1118|Compound:chelidonine|FD-Score:3.37|P-value:3.13E-4 ID:SGTC_1233|Compound:0325-0408|FD-Score:3.36|P-value:3.25E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:3.34|P-value:3.49E-4 ID:SGTC_1451|Compound:4152-0210|FD-Score:3.37|P-value:3.10E-4 ID:SGTC_1452|Compound:4194-0072|FD-Score:3.68|P-value:9.48E-5 ID:SGTC_1492|Compound:4158-0558|FD-Score:3.45|P-value:2.30E-4 ID:SGTC_1605|Compound:st000082|FD-Score:3.27|P-value:4.59E-4 ID:SGTC_1629|Compound:st005085|FD-Score:-5.99|P-value:6.24E-10 ID:SGTC_1635|Compound:st007035|FD-Score:-4.03|P-value:2.19E-5 ID:SGTC_1637|Compound:st007924|FD-Score:-12.70|P-value:2.45E-38 ID:SGTC_1731|Compound:st035964|FD-Score:3.22|P-value:5.40E-4 ID:SGTC_1866|Compound:st057638|FD-Score:3.87|P-value:4.31E-5 ID:SGTC_2064|Compound:5220758|FD-Score:3.84|P-value:4.94E-5 ID:SGTC_2394|Compound:5221648|FD-Score:4.58|P-value:1.68E-6 ID:SGTC_2399|Compound:5884133|FD-Score:-3.08|P-value:8.99E-4 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:-3.43|P-value:2.49E-4 ID:SGTC_2446|Compound:5162541|FD-Score:3.17|P-value:6.55E-4 ID:SGTC_2483|Compound:5729050|FD-Score:-3.27|P-value:4.50E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:4.01|P-value:2.40E-5 ID:SGTC_2706|Compound:st078239|FD-Score:3.35|P-value:3.32E-4 ID:SGTC_2715|Compound:st079500|FD-Score:3.08|P-value:8.82E-4 ID:SGTC_2783|Compound:7738165|FD-Score:-3.32|P-value:3.80E-4 ID:SGTC_2784|Compound:7567673|FD-Score:-3.61|P-value:1.24E-4 ID:SGTC_2788|Compound:5282734|FD-Score:4.67|P-value:1.07E-6 ID:SGTC_2835|Compound:9006588|FD-Score:-4.61|P-value:1.48E-6 ID:SGTC_3027|Compound:9089541|FD-Score:-3.40|P-value:2.80E-4 ID:SGTC_3031|Compound:9088762|FD-Score:-4.09|P-value:1.67E-5 ID:SGTC_3057|Compound:9095946|FD-Score:3.61|P-value:1.23E-4 ID:SGTC_3221|Compound:9128209|FD-Score:4.23|P-value:8.74E-6 ID:SGTC_3260|Compound:9137109|FD-Score:4.26|P-value:7.64E-6 ID:SGTC_3273|Compound:9137859|FD-Score:3.89|P-value:3.99E-5 ID:SGTC_3282|Compound:9108498|FD-Score:3.49|P-value:2.00E-4 ID:SGTC_3306|Compound:9127676|FD-Score:3.10|P-value:8.33E-4

Top fitness defect scores for YEL047C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_6 0844-0013 5.31 19.8 µM 3.46E-8 27 5.35E-8
2 SGTC_840 0245-0196 5.02 390.0 µM 1.77E-7 4 2.62E-7
3 SGTC_2788 5282734 4.67 71.4 µM 1.07E-6 25 1.51E-6
4 SGTC_2394 5221648 4.58 200.0 µM 1.68E-6 12 2.34E-6
5 SGTC_3260 9137109 4.26 49.5 µM 7.64E-6 60S ribosome export 8 1.02E-5
6 SGTC_3221 9128209 4.23 49.5 µM 8.74E-6 9 1.16E-5
7 SGTC_965 1291-5240 4.23 308.0 µM 8.76E-6 26 1.16E-5
8 SGTC_380 3970-0968 4.15 6.0 µM 1.27E-5 9 1.67E-5
9 SGTC_2637 3-alpha-acetoxydihydrodeoxygedunin 4.01 100.0 µM 2.40E-5 23 3.09E-5
10 SGTC_98 0074-0023 3.91 107.7 µM 3.57E-5 Golgi 39 4.55E-5
11 SGTC_3273 9137859 3.89 49.5 µM 3.99E-5 6 5.07E-5
12 SGTC_1866 st057638 3.87 64.0 µM 4.31E-5 13 5.47E-5
13 SGTC_2064 5220758 3.84 200.0 µM 4.94E-5 39 6.25E-5
14 SGTC_401 neomycin 3.84 50.0 µM 4.97E-5 12 6.27E-5
15 SGTC_1452 4194-0072 3.68 190.0 µM 9.48E-5 20 1.18E-4
16 SGTC_3057 9095946 3.61 49.5 µM 1.23E-4 Golgi 21 1.52E-4
17 SGTC_853 0419-0306 3.54 21.0 µM 1.62E-4 26 1.98E-4
18 SGTC_3282 9108498 3.49 15.5 µM 2.00E-4 45 2.43E-4
19 SGTC_1492 4158-0558 3.45 28.5 µM 2.30E-4 60S ribosome export 24 2.78E-4
20 SGTC_1451 4152-0210 3.37 104.0 µM 3.10E-4 47 3.72E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.169 7.17E-23 YDL113C ATG20 hom Sorting nexin family member required for the cytoplasm-to-vacuole targeting (Cvt) pathway and for endosomal sorting; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate
0.147 1.14E-17 YKR032W_d YKR032W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.127 1.72E-13 YDL128W VCX1 hom Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity, involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter
0.119 4.05E-12 YDL098C SNU23 het Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome
0.118 8.61E-12 YKL061W_p BLI1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome
0.114 3.57E-11 YIL058W_d YIL058W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.109 2.59E-10 YKL094W YJU3 hom Monoglyceride lipase (MGL), functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family
0.107 5.81E-10 YLR164W SHH4 hom Mitochondrial inner membrane protein of unknown function; similar to Tim18p and Sdh4p; a fraction copurifies with Sdh3p, but Shh4p is neither a stoichiometric subunit of succinate dehydrogenase nor of the TIM22 translocase; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner
0.101 4.63E-9 YML086C ALO1 hom D-Arabinono-1,4-lactone oxidase, catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress
0.099 8.51E-9 YPR035W GLN1 het Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
0.097 1.52E-8 YKL164C PIR1 hom O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication
0.092 8.72E-8 YDL049C KNH1 hom Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
0.090 1.74E-7 YBR220C_p YBR220C_p hom Putative protein of unknown function; YBR220C is not an essential gene
0.090 1.74E-7 YDL060W TSR1 het Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.090 1.81E-7 YFL046W_p FMP32_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies