YER009W / NTF2

Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport

Zygosity: Heterozygous strain
fixedexpanded
Profile for YER009W / NTF2

Click on Significant Values for Screen Details ID:SGTC_50|Compound:0929-0078|FD-Score:3.20|P-value:4.57E-4 ID:SGTC_194|Compound:1486-1323|FD-Score:-3.66|P-value:8.16E-5 ID:SGTC_196|Compound:k015-0023|FD-Score:-3.36|P-value:2.70E-4 ID:SGTC_199|Compound:4063-0024|FD-Score:-3.07|P-value:7.74E-4 ID:SGTC_532|Compound:1548-0774|FD-Score:3.06|P-value:7.53E-4 ID:SGTC_533|Compound:1222-0222|FD-Score:3.00|P-value:9.46E-4 ID:SGTC_724|Compound:3910-0327|FD-Score:3.30|P-value:3.13E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:3.66|P-value:7.63E-5 ID:SGTC_769|Compound:0302-0231|FD-Score:11.40|P-value:4.19E-32 ID:SGTC_898|Compound:1000-0849|FD-Score:3.57|P-value:1.09E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:3.02|P-value:8.65E-4 ID:SGTC_909|Compound:3448-9333|FD-Score:3.80|P-value:4.14E-5 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:3.45|P-value:1.75E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:-3.27|P-value:3.83E-4 ID:SGTC_970|Compound:1315-0089|FD-Score:4.18|P-value:7.52E-6 ID:SGTC_1042|Compound:0350-0096|FD-Score:4.63|P-value:8.14E-7 ID:SGTC_1044|Compound:1222-0021|FD-Score:3.05|P-value:7.83E-4 ID:SGTC_1208|Compound:2323-2347|FD-Score:3.26|P-value:3.65E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:3.01|P-value:8.91E-4 ID:SGTC_1682|Compound:st012968|FD-Score:3.04|P-value:8.07E-4 ID:SGTC_2008|Compound:4012253|FD-Score:-3.28|P-value:3.68E-4 ID:SGTC_2052|Compound:5265182|FD-Score:-3.82|P-value:4.10E-5 ID:SGTC_2128|Compound:5255449|FD-Score:4.88|P-value:2.20E-7 ID:SGTC_2161|Compound:5662504|FD-Score:3.32|P-value:2.88E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:-3.42|P-value:2.09E-4 ID:SGTC_2683|Compound:piperine|FD-Score:3.19|P-value:4.73E-4 ID:SGTC_2844|Compound:9010103|FD-Score:5.77|P-value:1.20E-9 ID:SGTC_2849|Compound:9019321|FD-Score:4.64|P-value:8.00E-7 ID:SGTC_2954|Compound:9074818|FD-Score:-3.21|P-value:4.77E-4 ID:SGTC_2974|Compound:9094440|FD-Score:3.50|P-value:1.45E-4 ID:SGTC_3291|Compound:9121177|FD-Score:3.09|P-value:6.94E-4 ID:SGTC_3343|Compound:9148379|FD-Score:-3.08|P-value:7.49E-4 ID:SGTC_50|Compound:0929-0078|FD-Score:3.20|P-value:4.57E-4 ID:SGTC_194|Compound:1486-1323|FD-Score:-3.66|P-value:8.16E-5 ID:SGTC_196|Compound:k015-0023|FD-Score:-3.36|P-value:2.70E-4 ID:SGTC_199|Compound:4063-0024|FD-Score:-3.07|P-value:7.74E-4 ID:SGTC_532|Compound:1548-0774|FD-Score:3.06|P-value:7.53E-4 ID:SGTC_533|Compound:1222-0222|FD-Score:3.00|P-value:9.46E-4 ID:SGTC_724|Compound:3910-0327|FD-Score:3.30|P-value:3.13E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:3.66|P-value:7.63E-5 ID:SGTC_769|Compound:0302-0231|FD-Score:11.40|P-value:4.19E-32 ID:SGTC_898|Compound:1000-0849|FD-Score:3.57|P-value:1.09E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:3.02|P-value:8.65E-4 ID:SGTC_909|Compound:3448-9333|FD-Score:3.80|P-value:4.14E-5 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:3.45|P-value:1.75E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:-3.27|P-value:3.83E-4 ID:SGTC_970|Compound:1315-0089|FD-Score:4.18|P-value:7.52E-6 ID:SGTC_1042|Compound:0350-0096|FD-Score:4.63|P-value:8.14E-7 ID:SGTC_1044|Compound:1222-0021|FD-Score:3.05|P-value:7.83E-4 ID:SGTC_1208|Compound:2323-2347|FD-Score:3.26|P-value:3.65E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:3.01|P-value:8.91E-4 ID:SGTC_1682|Compound:st012968|FD-Score:3.04|P-value:8.07E-4 ID:SGTC_2008|Compound:4012253|FD-Score:-3.28|P-value:3.68E-4 ID:SGTC_2052|Compound:5265182|FD-Score:-3.82|P-value:4.10E-5 ID:SGTC_2128|Compound:5255449|FD-Score:4.88|P-value:2.20E-7 ID:SGTC_2161|Compound:5662504|FD-Score:3.32|P-value:2.88E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:-3.42|P-value:2.09E-4 ID:SGTC_2683|Compound:piperine|FD-Score:3.19|P-value:4.73E-4 ID:SGTC_2844|Compound:9010103|FD-Score:5.77|P-value:1.20E-9 ID:SGTC_2849|Compound:9019321|FD-Score:4.64|P-value:8.00E-7 ID:SGTC_2954|Compound:9074818|FD-Score:-3.21|P-value:4.77E-4 ID:SGTC_2974|Compound:9094440|FD-Score:3.50|P-value:1.45E-4 ID:SGTC_3291|Compound:9121177|FD-Score:3.09|P-value:6.94E-4 ID:SGTC_3343|Compound:9148379|FD-Score:-3.08|P-value:7.49E-4

Top fitness defect scores for YER009W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_769 0302-0231 11.40 76.0 µM 4.19E-32 1 3.68E-30 5.87
2 SGTC_2844 9010103 5.77 26.0 µM 1.20E-9 4 4.00E-9 1.30
3 SGTC_2128 5255449 4.88 168.5 µM 2.20E-7 1 5.30E-7 0.38
4 SGTC_2849 9019321 4.64 13.0 µM 8.00E-7 4 1.78E-6 0.11
5 SGTC_1042 0350-0096 4.63 82.0 µM 8.14E-7 TSC3-RPN4 5 1.81E-6 0.29
6 SGTC_970 1315-0089 4.18 19.5 µM 7.52E-6 RSC & ERG11 8 1.46E-5 0.12
7 SGTC_909 3448-9333 3.80 149.0 µM 4.14E-5 2 7.22E-5 0.31
8 SGTC_757 1066-0008 3.66 322.0 µM 7.63E-5 Golgi 5 1.28E-4 0.03
9 SGTC_898 1000-0849 3.57 28.3 µM 1.09E-4 3 1.79E-4 0.01
10 SGTC_2974 9094440 3.50 9.7 µM 1.45E-4 ergosterol biosynthesis 21 2.34E-4 0.00
11 SGTC_915 methyl methanesulfonate (MMS) 3.45 110.0 mM 1.75E-4 DNA damage response 13 2.79E-4 0.09
12 SGTC_2161 5662504 3.32 8.5 µM 2.88E-4 tubulin folding & SWR complex 12 4.45E-4 0.01
13 SGTC_724 3910-0327 3.30 137.0 µM 3.13E-4 ERG2 10 4.82E-4 0.06
14 SGTC_1208 2323-2347 3.26 50.3 µM 3.65E-4 2 5.57E-4 0.22
15 SGTC_50 0929-0078 3.20 56.4 µM 4.57E-4 11 6.87E-4 0.20
16 SGTC_2683 piperine 3.19 63.0 µM 4.73E-4 6 7.09E-4 0.17
17 SGTC_3291 9121177 3.09 71.4 µM 6.94E-4 5 0.00102 0.06
18 SGTC_532 1548-0774 3.06 215.0 µM 7.53E-4 13 0.00110 0.02
19 SGTC_1044 1222-0021 3.05 1.8 µM 7.83E-4 heme biosynthesis & mitochondrial translocase 51 0.00114 0.07
20 SGTC_1682 st012968 3.04 530.0 nM 8.07E-4 13 0.00117 0.12

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.265 3.14E-55 YMR115W MGR3 hom Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA
0.201 4.71E-32 YER172C BRR2 het RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD
0.175 1.83E-24 YOR166C SWT1 hom RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain
0.164 1.55E-21 YMR101C SRT1 hom Cis-prenyltransferase involved in synthesis of long-chain dolichols (19-22 isoprene units; as opposed to Rer2p which synthesizes shorter-chain dolichols); localizes to lipid bodies; transcription is induced during stationary phase
0.149 4.56E-18 YBR262C AIM5 hom Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture
0.144 4.31E-17 YEL025C_p YEL025C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
0.141 2.25E-16 YNL027W CRZ1 hom Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress
0.137 2.00E-15 YLL026W HSP104 hom Disaggregase; heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress
0.136 2.27E-15 YNL277W-A_p YNL277W-A_p hom Putative protein of unknown function
0.128 9.34E-14 YDL023C_d YDL023C_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in other Saccharomyces species; overlaps the verified gene GPD1; deletion confers sensitivity to GSAO; deletion in cyr1 mutant results in loss of stress resistance
0.127 1.39E-13 YIL140W AXL2 hom Integral plasma membrane protein required for axial budding in haploid cells, localizes to the incipient bud site and bud neck; glycosylated by Pmt4p; potential Cdc28p substrate
0.121 2.43E-12 YHL014C YLF2 hom Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.119 4.45E-12 YLR325C RPL38 hom Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog
0.117 1.15E-11 YPR133W-A TOM5 hom Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore
0.115 2.12E-11 YPL221W FLC1 hom Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance