YER014C-A / BUD25

Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern

Zygosity: Homozygous strain
fixedexpanded
Profile for YER014C-A / BUD25

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:-4.47|P-value:1.03E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.48|P-value:4.75E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:-3.33|P-value:8.04E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:-4.08|P-value:5.15E-5 ID:SGTC_367|Compound:0335-0881|FD-Score:-4.18|P-value:3.50E-5 ID:SGTC_370|Compound:0180-0345|FD-Score:3.84|P-value:7.31E-5 ID:SGTC_449|Compound:xestospongin c|FD-Score:3.78|P-value:9.16E-5 ID:SGTC_462|Compound:ag-879|FD-Score:3.24|P-value:6.48E-4 ID:SGTC_536|Compound:3852-0578|FD-Score:-3.77|P-value:1.71E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:-4.03|P-value:6.31E-5 ID:SGTC_611|Compound:3453-1652|FD-Score:-3.46|P-value:5.18E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:-3.60|P-value:3.11E-4 ID:SGTC_843|Compound:0310-0051|FD-Score:-3.64|P-value:2.73E-4 ID:SGTC_904|Compound:1141-0088|FD-Score:-3.41|P-value:6.03E-4 ID:SGTC_948|Compound:3448-0295|FD-Score:3.46|P-value:3.03E-4 ID:SGTC_1172|Compound:0370-0043|FD-Score:-4.22|P-value:2.98E-5 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.42|P-value:3.51E-4 ID:SGTC_1246|Compound:0422-0085|FD-Score:3.45|P-value:3.10E-4 ID:SGTC_1280|Compound:0880-0253|FD-Score:-3.46|P-value:5.11E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:-4.50|P-value:9.10E-6 ID:SGTC_1320|Compound:1313-0481|FD-Score:-5.42|P-value:1.09E-7 ID:SGTC_1545|Compound:lovastatin|FD-Score:-4.19|P-value:3.30E-5 ID:SGTC_1547|Compound:harmine|FD-Score:3.43|P-value:3.34E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:-3.40|P-value:6.36E-4 ID:SGTC_1807|Compound:st050092|FD-Score:3.44|P-value:3.27E-4 ID:SGTC_1891|Compound:st059073|FD-Score:3.20|P-value:7.42E-4 ID:SGTC_1917|Compound:st066507|FD-Score:-3.35|P-value:7.41E-4 ID:SGTC_1960|Compound:st011366|FD-Score:3.41|P-value:3.65E-4 ID:SGTC_2014|Compound:4013930|FD-Score:-3.46|P-value:5.25E-4 ID:SGTC_2097|Compound:5376186|FD-Score:-4.07|P-value:5.33E-5 ID:SGTC_2130|Compound:NSC 7|FD-Score:-3.37|P-value:6.96E-4 ID:SGTC_2343|Compound:7990716|FD-Score:-3.69|P-value:2.26E-4 ID:SGTC_2401|Compound:5128683|FD-Score:-3.59|P-value:3.22E-4 ID:SGTC_2403|Compound:6032726|FD-Score:3.70|P-value:1.23E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:3.43|P-value:3.30E-4 ID:SGTC_2436|Compound:5633522|FD-Score:3.32|P-value:4.99E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:-4.13|P-value:4.20E-5 ID:SGTC_2761|Compound:levamisole|FD-Score:-3.85|P-value:1.25E-4 ID:SGTC_2905|Compound:7799065|FD-Score:-3.91|P-value:1.02E-4 ID:SGTC_2940|Compound:9060638|FD-Score:-3.36|P-value:7.20E-4 ID:SGTC_3004|Compound:9076122|FD-Score:-3.83|P-value:1.37E-4 ID:SGTC_3007|Compound:9078383|FD-Score:4.11|P-value:2.48E-5 ID:SGTC_3014|Compound:9081533|FD-Score:-3.77|P-value:1.71E-4 ID:SGTC_3125|Compound:9127534|FD-Score:-3.40|P-value:6.37E-4 ID:SGTC_3133|Compound:9127736|FD-Score:3.28|P-value:5.74E-4 ID:SGTC_3289|Compound:9119912|FD-Score:-3.41|P-value:6.20E-4 ID:SGTC_3299|Compound:9121982|FD-Score:3.16|P-value:8.50E-4 ID:SGTC_3300|Compound:9124615|FD-Score:4.05|P-value:3.12E-5 ID:SGTC_3315|Compound:9136395|FD-Score:-3.43|P-value:5.75E-4 ID:SGTC_3325|Compound:9138760|FD-Score:-6.56|P-value:1.61E-10 ID:SGTC_36|Compound:0335-0881|FD-Score:-4.47|P-value:1.03E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.48|P-value:4.75E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:-3.33|P-value:8.04E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:-4.08|P-value:5.15E-5 ID:SGTC_367|Compound:0335-0881|FD-Score:-4.18|P-value:3.50E-5 ID:SGTC_370|Compound:0180-0345|FD-Score:3.84|P-value:7.31E-5 ID:SGTC_449|Compound:xestospongin c|FD-Score:3.78|P-value:9.16E-5 ID:SGTC_462|Compound:ag-879|FD-Score:3.24|P-value:6.48E-4 ID:SGTC_536|Compound:3852-0578|FD-Score:-3.77|P-value:1.71E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:-4.03|P-value:6.31E-5 ID:SGTC_611|Compound:3453-1652|FD-Score:-3.46|P-value:5.18E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:-3.60|P-value:3.11E-4 ID:SGTC_843|Compound:0310-0051|FD-Score:-3.64|P-value:2.73E-4 ID:SGTC_904|Compound:1141-0088|FD-Score:-3.41|P-value:6.03E-4 ID:SGTC_948|Compound:3448-0295|FD-Score:3.46|P-value:3.03E-4 ID:SGTC_1172|Compound:0370-0043|FD-Score:-4.22|P-value:2.98E-5 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.42|P-value:3.51E-4 ID:SGTC_1246|Compound:0422-0085|FD-Score:3.45|P-value:3.10E-4 ID:SGTC_1280|Compound:0880-0253|FD-Score:-3.46|P-value:5.11E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:-4.50|P-value:9.10E-6 ID:SGTC_1320|Compound:1313-0481|FD-Score:-5.42|P-value:1.09E-7 ID:SGTC_1545|Compound:lovastatin|FD-Score:-4.19|P-value:3.30E-5 ID:SGTC_1547|Compound:harmine|FD-Score:3.43|P-value:3.34E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:-3.40|P-value:6.36E-4 ID:SGTC_1807|Compound:st050092|FD-Score:3.44|P-value:3.27E-4 ID:SGTC_1891|Compound:st059073|FD-Score:3.20|P-value:7.42E-4 ID:SGTC_1917|Compound:st066507|FD-Score:-3.35|P-value:7.41E-4 ID:SGTC_1960|Compound:st011366|FD-Score:3.41|P-value:3.65E-4 ID:SGTC_2014|Compound:4013930|FD-Score:-3.46|P-value:5.25E-4 ID:SGTC_2097|Compound:5376186|FD-Score:-4.07|P-value:5.33E-5 ID:SGTC_2130|Compound:NSC 7|FD-Score:-3.37|P-value:6.96E-4 ID:SGTC_2343|Compound:7990716|FD-Score:-3.69|P-value:2.26E-4 ID:SGTC_2401|Compound:5128683|FD-Score:-3.59|P-value:3.22E-4 ID:SGTC_2403|Compound:6032726|FD-Score:3.70|P-value:1.23E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:3.43|P-value:3.30E-4 ID:SGTC_2436|Compound:5633522|FD-Score:3.32|P-value:4.99E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:-4.13|P-value:4.20E-5 ID:SGTC_2761|Compound:levamisole|FD-Score:-3.85|P-value:1.25E-4 ID:SGTC_2905|Compound:7799065|FD-Score:-3.91|P-value:1.02E-4 ID:SGTC_2940|Compound:9060638|FD-Score:-3.36|P-value:7.20E-4 ID:SGTC_3004|Compound:9076122|FD-Score:-3.83|P-value:1.37E-4 ID:SGTC_3007|Compound:9078383|FD-Score:4.11|P-value:2.48E-5 ID:SGTC_3014|Compound:9081533|FD-Score:-3.77|P-value:1.71E-4 ID:SGTC_3125|Compound:9127534|FD-Score:-3.40|P-value:6.37E-4 ID:SGTC_3133|Compound:9127736|FD-Score:3.28|P-value:5.74E-4 ID:SGTC_3289|Compound:9119912|FD-Score:-3.41|P-value:6.20E-4 ID:SGTC_3299|Compound:9121982|FD-Score:3.16|P-value:8.50E-4 ID:SGTC_3300|Compound:9124615|FD-Score:4.05|P-value:3.12E-5 ID:SGTC_3315|Compound:9136395|FD-Score:-3.43|P-value:5.75E-4 ID:SGTC_3325|Compound:9138760|FD-Score:-6.56|P-value:1.61E-10

Top fitness defect scores for YER014C-A deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3007 9078383 4.11 71.4 µM 2.48E-5 12 2.01E-5
2 SGTC_3300 9124615 4.05 50.5 µM 3.12E-5 18 2.55E-5
3 SGTC_370 0180-0345 3.84 38.7 µM 7.31E-5 DNA intercalators 13 6.18E-5
4 SGTC_449 xestospongin c 3.78 11.2 µM 9.16E-5 NEO1-PIK1 76 7.81E-5
5 SGTC_2403 6032726 3.70 181.2 µM 1.23E-4 44 1.07E-4
6 SGTC_948 3448-0295 3.46 75.3 µM 3.03E-4 46 2.71E-4
7 SGTC_1246 0422-0085 3.45 105.0 µM 3.10E-4 54 2.78E-4
8 SGTC_1807 st050092 3.44 58.9 µM 3.27E-4 60S ribosome export 25 2.93E-4
9 SGTC_2422 chlorphenesin carbamate 3.43 384.2 µM 3.30E-4 51 2.96E-4
10 SGTC_1547 harmine 3.43 94.2 µM 3.34E-4 ergosterol biosynthesis 67 3.00E-4
11 SGTC_1239 0337-0584 3.42 35.4 µM 3.51E-4 unfolded protein response 79 3.16E-4
12 SGTC_1960 st011366 3.41 51.8 µM 3.65E-4 24 3.29E-4
13 SGTC_2436 5633522 3.32 8.1 µM 4.99E-4 34 4.55E-4
14 SGTC_3133 9127736 3.28 49.5 µM 5.74E-4 43 5.27E-4
15 SGTC_462 ag-879 3.24 158.0 µM 6.48E-4 56 5.97E-4
16 SGTC_1891 st059073 3.20 26.2 µM 7.42E-4 TSC3-RPN4 46 6.87E-4
17 SGTC_3299 9121982 3.16 43.1 µM 8.50E-4 65 7.91E-4
18 SGTC_22 0964-0034 3.07 14.5 µM 0.00115 unfolded protein response 87 0.00108
19 SGTC_657 3534-0529 3.01 86.7 µM 0.00138 47 0.00130
20 SGTC_2476 5806916 2.99 10.5 µM 0.00146 54 0.00138

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.088 3.29E-7 YBR080C SEC18 het ATPase required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, autophagy, and protein secretion; releases Sec17p from SNAP complexes; has similarity to mammalian NSF
0.084 9.89E-7 YAL032C PRP45 het Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene
0.081 2.37E-6 YML130C ERO1 het Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds
0.081 2.90E-6 YBR289W SNF5 hom Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions
0.076 9.72E-6 YML054C-A_p YML054C-A_p hom Putative protein of unknown function
0.076 1.03E-5 YPR150W_d YPR150W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SUE1/YPR151C
0.075 1.34E-5 YPR106W ISR1 hom Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C
0.071 4.13E-5 YLR217W_d YLR217W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CPR6
0.066 1.34E-4 YER066C-A_d YER066C-A_d hom Dubious open reading frame unlikely to encode a protein, partially overlaps uncharacterized ORF YER067W
0.065 1.53E-4 YNR030W ALG12 hom Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation
0.065 1.69E-4 YML038C YMD8 hom Putative nucleotide sugar transporter, has similarity to Vrg4p
0.063 2.72E-4 YHR059W FYV4 hom Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.062 3.20E-4 YHR004C NEM1 hom Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard
0.062 3.30E-4 YNL143C_p YNL143C_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.062 3.37E-4 YPL160W CDC60 het Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA