YER019W / ISC1

Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance

Zygosity: Homozygous strain
fixedexpanded
Profile for YER019W / ISC1

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-3.64|P-value:1.86E-4 ID:SGTC_75|Compound:0159-0059|FD-Score:3.72|P-value:1.53E-4 ID:SGTC_79|Compound:0052-0007|FD-Score:-3.98|P-value:5.16E-5 ID:SGTC_99|Compound:0109-0120|FD-Score:3.36|P-value:5.61E-4 ID:SGTC_101|Compound:0490-5701|FD-Score:-3.22|P-value:8.41E-4 ID:SGTC_139|Compound:0099-0334|FD-Score:3.94|P-value:6.61E-5 ID:SGTC_210|Compound:3551-0445|FD-Score:4.62|P-value:3.70E-6 ID:SGTC_268|Compound:camptothecin|FD-Score:3.36|P-value:5.56E-4 ID:SGTC_532|Compound:1548-0774|FD-Score:4.32|P-value:1.37E-5 ID:SGTC_537|Compound:1334-0398|FD-Score:5.89|P-value:5.25E-9 ID:SGTC_670|Compound:4204-0025|FD-Score:3.72|P-value:1.56E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:3.56|P-value:2.78E-4 ID:SGTC_821|Compound:1114-0266|FD-Score:3.38|P-value:5.30E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:-3.36|P-value:5.11E-4 ID:SGTC_974|Compound:1326-0144|FD-Score:3.68|P-value:1.83E-4 ID:SGTC_1042|Compound:0350-0096|FD-Score:4.45|P-value:7.94E-6 ID:SGTC_1049|Compound:3226-0134|FD-Score:3.75|P-value:1.38E-4 ID:SGTC_1160|Compound:4092-0570|FD-Score:3.47|P-value:3.89E-4 ID:SGTC_1163|Compound:k060-0048|FD-Score:3.34|P-value:6.04E-4 ID:SGTC_1272|Compound:0839-0021|FD-Score:3.42|P-value:4.52E-4 ID:SGTC_1341|Compound:1486-1360|FD-Score:3.52|P-value:3.27E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:3.58|P-value:2.62E-4 ID:SGTC_1362|Compound:1611-0397|FD-Score:-3.31|P-value:6.04E-4 ID:SGTC_1458|Compound:k015-0034|FD-Score:-3.90|P-value:7.06E-5 ID:SGTC_1479|Compound:0549-0158|FD-Score:3.54|P-value:3.04E-4 ID:SGTC_1484|Compound:3473-2379|FD-Score:3.45|P-value:4.07E-4 ID:SGTC_1581|Compound:himbacine|FD-Score:-4.35|P-value:1.08E-5 ID:SGTC_1725|Compound:st036284|FD-Score:3.41|P-value:4.68E-4 ID:SGTC_1784|Compound:st039334|FD-Score:4.42|P-value:8.89E-6 ID:SGTC_1794|Compound:st048918|FD-Score:3.40|P-value:5.00E-4 ID:SGTC_1997|Compound:st073024|FD-Score:3.44|P-value:4.35E-4 ID:SGTC_2010|Compound:4012422|FD-Score:3.32|P-value:6.53E-4 ID:SGTC_2056|Compound:5235370|FD-Score:3.96|P-value:6.16E-5 ID:SGTC_2089|Compound:5281248|FD-Score:3.96|P-value:6.06E-5 ID:SGTC_2090|Compound:5301565|FD-Score:5.22|P-value:2.06E-7 ID:SGTC_2092|Compound:5303694|FD-Score:4.62|P-value:3.71E-6 ID:SGTC_2526|Compound:patulin|FD-Score:3.55|P-value:2.93E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:-3.25|P-value:7.51E-4 ID:SGTC_2681|Compound:menadione|FD-Score:3.52|P-value:3.20E-4 ID:SGTC_2885|Compound:9057732|FD-Score:-7.11|P-value:2.10E-12 ID:SGTC_2893|Compound:9048488|FD-Score:-5.21|P-value:1.89E-7 ID:SGTC_2897|Compound:9048598|FD-Score:3.84|P-value:9.67E-5 ID:SGTC_2908|Compound:7930301|FD-Score:3.53|P-value:3.15E-4 ID:SGTC_2914|Compound:7968218|FD-Score:4.27|P-value:1.70E-5 ID:SGTC_3143|Compound:9086658|FD-Score:-4.88|P-value:9.53E-7 ID:SGTC_3151|Compound:9099821|FD-Score:3.52|P-value:3.27E-4 ID:SGTC_3193|Compound:9111931|FD-Score:-3.87|P-value:7.75E-5 ID:SGTC_3232|Compound:9132874|FD-Score:3.51|P-value:3.33E-4 ID:SGTC_3344|Compound:9148862|FD-Score:3.57|P-value:2.70E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.64|P-value:1.86E-4 ID:SGTC_75|Compound:0159-0059|FD-Score:3.72|P-value:1.53E-4 ID:SGTC_79|Compound:0052-0007|FD-Score:-3.98|P-value:5.16E-5 ID:SGTC_99|Compound:0109-0120|FD-Score:3.36|P-value:5.61E-4 ID:SGTC_101|Compound:0490-5701|FD-Score:-3.22|P-value:8.41E-4 ID:SGTC_139|Compound:0099-0334|FD-Score:3.94|P-value:6.61E-5 ID:SGTC_210|Compound:3551-0445|FD-Score:4.62|P-value:3.70E-6 ID:SGTC_268|Compound:camptothecin|FD-Score:3.36|P-value:5.56E-4 ID:SGTC_532|Compound:1548-0774|FD-Score:4.32|P-value:1.37E-5 ID:SGTC_537|Compound:1334-0398|FD-Score:5.89|P-value:5.25E-9 ID:SGTC_670|Compound:4204-0025|FD-Score:3.72|P-value:1.56E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:3.56|P-value:2.78E-4 ID:SGTC_821|Compound:1114-0266|FD-Score:3.38|P-value:5.30E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:-3.36|P-value:5.11E-4 ID:SGTC_974|Compound:1326-0144|FD-Score:3.68|P-value:1.83E-4 ID:SGTC_1042|Compound:0350-0096|FD-Score:4.45|P-value:7.94E-6 ID:SGTC_1049|Compound:3226-0134|FD-Score:3.75|P-value:1.38E-4 ID:SGTC_1160|Compound:4092-0570|FD-Score:3.47|P-value:3.89E-4 ID:SGTC_1163|Compound:k060-0048|FD-Score:3.34|P-value:6.04E-4 ID:SGTC_1272|Compound:0839-0021|FD-Score:3.42|P-value:4.52E-4 ID:SGTC_1341|Compound:1486-1360|FD-Score:3.52|P-value:3.27E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:3.58|P-value:2.62E-4 ID:SGTC_1362|Compound:1611-0397|FD-Score:-3.31|P-value:6.04E-4 ID:SGTC_1458|Compound:k015-0034|FD-Score:-3.90|P-value:7.06E-5 ID:SGTC_1479|Compound:0549-0158|FD-Score:3.54|P-value:3.04E-4 ID:SGTC_1484|Compound:3473-2379|FD-Score:3.45|P-value:4.07E-4 ID:SGTC_1581|Compound:himbacine|FD-Score:-4.35|P-value:1.08E-5 ID:SGTC_1725|Compound:st036284|FD-Score:3.41|P-value:4.68E-4 ID:SGTC_1784|Compound:st039334|FD-Score:4.42|P-value:8.89E-6 ID:SGTC_1794|Compound:st048918|FD-Score:3.40|P-value:5.00E-4 ID:SGTC_1997|Compound:st073024|FD-Score:3.44|P-value:4.35E-4 ID:SGTC_2010|Compound:4012422|FD-Score:3.32|P-value:6.53E-4 ID:SGTC_2056|Compound:5235370|FD-Score:3.96|P-value:6.16E-5 ID:SGTC_2089|Compound:5281248|FD-Score:3.96|P-value:6.06E-5 ID:SGTC_2090|Compound:5301565|FD-Score:5.22|P-value:2.06E-7 ID:SGTC_2092|Compound:5303694|FD-Score:4.62|P-value:3.71E-6 ID:SGTC_2526|Compound:patulin|FD-Score:3.55|P-value:2.93E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:-3.25|P-value:7.51E-4 ID:SGTC_2681|Compound:menadione|FD-Score:3.52|P-value:3.20E-4 ID:SGTC_2885|Compound:9057732|FD-Score:-7.11|P-value:2.10E-12 ID:SGTC_2893|Compound:9048488|FD-Score:-5.21|P-value:1.89E-7 ID:SGTC_2897|Compound:9048598|FD-Score:3.84|P-value:9.67E-5 ID:SGTC_2908|Compound:7930301|FD-Score:3.53|P-value:3.15E-4 ID:SGTC_2914|Compound:7968218|FD-Score:4.27|P-value:1.70E-5 ID:SGTC_3143|Compound:9086658|FD-Score:-4.88|P-value:9.53E-7 ID:SGTC_3151|Compound:9099821|FD-Score:3.52|P-value:3.27E-4 ID:SGTC_3193|Compound:9111931|FD-Score:-3.87|P-value:7.75E-5 ID:SGTC_3232|Compound:9132874|FD-Score:3.51|P-value:3.33E-4 ID:SGTC_3344|Compound:9148862|FD-Score:3.57|P-value:2.70E-4

Top fitness defect scores for YER019W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_537 1334-0398 5.89 209.0 µM 5.25E-9 4 1.88E-9
2 SGTC_2090 5301565 5.22 104.5 µM 2.06E-7 TSC3-RPN4 25 9.10E-8
3 SGTC_210 3551-0445 4.62 56.8 µM 3.70E-6 47 1.92E-6
4 SGTC_2092 5303694 4.62 80.7 µM 3.71E-6 TSC3-RPN4 20 1.93E-6
5 SGTC_1042 0350-0096 4.45 82.0 µM 7.94E-6 TSC3-RPN4 15 4.31E-6
6 SGTC_1784 st039334 4.42 36.3 µM 8.89E-6 11 4.86E-6
7 SGTC_532 1548-0774 4.32 215.0 µM 1.37E-5 12 7.66E-6
8 SGTC_2914 7968218 4.27 45.3 µM 1.70E-5 10 9.63E-6
9 SGTC_2089 5281248 3.96 129.7 µM 6.06E-5 superoxide 20 3.69E-5
10 SGTC_2056 5235370 3.96 200.0 µM 6.16E-5 40 3.75E-5
11 SGTC_139 0099-0334 3.94 718.6 µM 6.61E-5 29 4.05E-5
12 SGTC_2897 9048598 3.84 13.0 µM 9.67E-5 18 6.05E-5
13 SGTC_1049 3226-0134 3.75 66.0 µM 1.38E-4 34 8.81E-5
14 SGTC_75 0159-0059 3.72 199.0 µM 1.53E-4 fatty acid desaturase (OLE1) 26 9.79E-5
15 SGTC_670 4204-0025 3.72 119.0 µM 1.56E-4 copper-dependent oxidative stress 37 1.00E-4
16 SGTC_974 1326-0144 3.68 138.0 µM 1.83E-4 ERG2 52 1.19E-4
17 SGTC_1353 1498-1206 3.58 8.1 µM 2.62E-4 mitochondrial response to ROS 69 1.73E-4
18 SGTC_3344 9148862 3.57 3.9 µM 2.70E-4 20 1.79E-4
19 SGTC_757 1066-0008 3.56 322.0 µM 2.78E-4 Golgi 29 1.84E-4
20 SGTC_2526 patulin 3.55 37.6 µM 2.93E-4 116 1.95E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.111 1.28E-10 YER107C GLE2 hom RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1
0.097 1.69E-8 YML007W YAP1 hom Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.092 9.56E-8 YPL241C CIN2 hom GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2
0.091 1.26E-7 YAL024C LTE1 hom Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures
0.087 4.50E-7 YER007W PAC2 hom Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
0.084 1.08E-6 YOR349W CIN1 hom Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl
0.084 1.10E-6 YOR036W PEP12 hom Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
0.084 1.10E-6 YNL214W PEX17 hom Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis
0.083 1.53E-6 YER005W YND1 hom Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity
0.080 3.70E-6 YOR233W KIN4 hom Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication
0.077 8.27E-6 YLR135W SLX4 hom Endonuclease involved in processing DNA; acts during recombination and repair; cleaves branched structures in a complex with Slx1p; involved interstrand cross-link repair and in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress
0.075 1.26E-5 YGR141W VPS62 hom Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins
0.073 2.05E-5 YGL061C DUO1 het Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
0.073 2.56E-5 YOR162C YRR1 hom Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication
0.072 2.87E-5 YBR008C FLR1 hom Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress