HIPHOP chemogenomics database

YER052C / HOM3

Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis

Zygosity: Homozygous strain

fixedexpanded
Profile for YER052C / HOM3

Click on Significant Values for Screen Details

Top fitness defect scores for YER052C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_163	1327-0036	41.00	7.5 µM	0		1	0
2	SGTC_7	0192-1582	37.20	4.2 µM	5.78E-299		1	2.41E-303
3	SGTC_2679	rapamycin	25.30	1.0 nM	5.98E-139		2	5.45E-141
4	SGTC_236	1509-0011	19.80	5.3 µM	3.06E-86		56	1.66E-87
5	SGTC_1844	rapamycin	18.20	4.0 nM	3.30E-73		7	2.78E-74
6	SGTC_2651	1327-0036	16.70	5.0 µM	2.76E-62		2	3.38E-63
7	SGTC_386	1509-0011	13.70	3.1 µM	3.69E-42		27	8.95E-43
8	SGTC_2233	sulbentine	8.13	40.4 µM	3.49E-16		13	2.06E-16
9	SGTC_107	0358-0022	6.82	2.7 µM	6.78E-12	SWF1 & branched chain AA biosynthesis	12	4.64E-12
10	SGTC_195	4234-0266	6.66	16.6 µM	2.01E-11		5	1.40E-11
11	SGTC_18	0111-0177	5.73	500.0 nM	6.54E-9		24	4.96E-9
12	SGTC_538	2922-0838	5.67	53.3 µM	9.24E-9	Golgi	8	7.05E-9
13	SGTC_371	0195-0006	5.46	7.4 µM	3.13E-8		5	2.43E-8
14	SGTC_34	0469-0683	5.33	81.8 µM	6.17E-8	SWF1 & branched chain AA biosynthesis	13	4.84E-8
15	SGTC_3324	9138759	5.30	60.8 µM	7.50E-8		5	5.90E-8
16	SGTC_2475	5812728	5.19	15.0 µM	1.30E-7	mitochondrial processes	9	1.03E-7
17	SGTC_2289	7946404	5.17	165.1 µM	1.51E-7		52	1.20E-7
18	SGTC_2469	5655019	4.68	90.8 µM	1.77E-6		14	1.46E-6
19	SGTC_3207	9114163	4.67	49.5 µM	1.86E-6		14	1.54E-6
20	SGTC_534	k292-0788	4.65	63.4 µM	2.01E-6	mitochondrial processes	15	1.67E-6

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.690	0	YCR053W	THR4	hom	Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway
0.472	4.70E-185	YDR158W	HOM2	hom	Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
0.404	9.44E-132	YGR208W	SER2	hom	Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source
0.397	8.94E-127	YJR013W	GPI14	het	Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
0.382	1.02E-116	YBR068C	BAP2	hom	High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication
0.377	6.55E-114	YHR025W	THR1	hom	Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway
0.372	1.67E-110	YJR139C	HOM6	hom	Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions
0.343	7.27E-93	YFL037W	TUB2	het	Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
0.341	9.22E-92	YIR008C	PRI1	het	Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
0.324	1.35E-82	YGR193C	PDX1	hom	Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core
0.322	9.11E-82	YOR184W	SER1	hom	3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress
0.317	3.79E-79	YBR049C	REB1	het	RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication
0.311	2.54E-76	YOR297C	TIM18	hom	Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex
0.302	1.38E-71	YOR274W	MOD5	hom	Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis
0.300	1.59E-70	YML121W	GTR1	hom	Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB