Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
Profile for YER053C-A / YER053C-A

Click on Significant Values for Screen Details

Top fitness defect scores for YER053C-A deletion by condition

RankScreen IDConditionFD ScoreConc
1 SGTC_2566 crystal violet carbinol 10.70 226.0 nM 2.80E-33 3 7.35E-27
2 SGTC_585 0kpi-0023 8.15 191.0 µM 3.31E-20 3 1.87E-16
3 SGTC_579 4491-1009 5.78 110.0 µM 4.80E-11 60S ribosome export 2 3.81E-9
4 SGTC_3213 9129884 4.64 49.5 µM 1.03E-7 11 1.77E-6
5 SGTC_1424 4048-1941 4.27 275.0 µM 8.78E-7 19 9.86E-6
6 SGTC_575 k072-0246 4.26 11.9 µM 9.23E-7 18 1.03E-5
7 SGTC_2285 7945142 4.23 200.0 µM 1.06E-6 16 1.15E-5
8 SGTC_1417 3966-0327 4.22 90.4 µM 1.13E-6 11 1.21E-5
9 SGTC_236 1509-0011 4.08 5.3 µM 2.46E-6 374 2.26E-5
10 SGTC_576 1073-0113 4.06 22.7 µM 2.67E-6 redox potentiating 60 2.41E-5
11 SGTC_1635 st007035 4.04 42.4 µM 3.04E-6 206 2.67E-5
12 SGTC_1723 st034307 4.02 2.5 µM 3.38E-6 mitochondrial processes 27 2.91E-5
13 SGTC_587 k089-0003 3.95 57.0 µM 4.97E-6 7 3.97E-5
14 SGTC_297 0986-0032 3.92 24.0 µM 5.74E-6 60S ribosome export 6 4.45E-5
15 SGTC_1576 erythromycin ethylsuccinate 3.78 23.2 µM 1.17E-5 22 7.88E-5
16 SGTC_567 1080-0542 3.76 68.4 µM 1.29E-5 14 8.52E-5
17 SGTC_120 rhodamine 6G 3.75 52.3 µM 1.34E-5 mitochondrial processes 41 8.80E-5
18 SGTC_2387 9077560 3.71 195.1 µM 1.66E-5 24 1.04E-4
19 SGTC_2711 st079103 3.60 59.1 µM 2.76E-5 10 1.57E-4
20 SGTC_520 3584-0023 3.40 682.0 µM 7.00E-5 42 3.34E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.185 3.31E-27 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.180 7.65E-26 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.179 1.39E-25 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.175 1.54E-24 YMR151W_d YIM2_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
0.169 8.21E-23 YEL059C-A SOM1 hom Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs
0.168 9.38E-23 YDR375C BCS1 hom Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
0.168 1.36E-22 YGR183C QCR9 hom Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex
0.167 1.71E-22 YGL191W COX13 hom Subunit VIa of cytochrome c oxidase; present in a subclass of cytochrome c oxidase complexes that may have a role in mimimizing generation of reactive oxygen species; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP; required for assembly of Rcf2p into cytochrome c oxidase - cytochrome bc1 supercomplexes
0.167 2.18E-22 YPL013C MRPS16 hom Mitochondrial ribosomal protein of the small subunit
0.166 4.69E-22 YGR174C CBP4 hom Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex
0.163 1.89E-21 YDR230W_d YDR230W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.160 9.37E-21 YER058W PET117 hom Protein required for assembly of cytochrome c oxidase
0.159 1.69E-20 YGL237C HAP2 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding
0.159 2.03E-20 YPL132W COX11 hom Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p
0.158 3.51E-20 YPL215W CBP3 hom Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex