YER063W / THO1

Conserved nuclear RNA-binding protein; specifically binds to transcribed chromatin in a THO- and RNA-dependent manner, genetically interacts with shuttling hnRNP NAB2; overproduction suppresses transcriptional defect caused by hpr1 mutation

Zygosity: Homozygous strain
fixedexpanded
Profile for YER063W / THO1

Click on Significant Values for Screen Details ID:SGTC_459|Compound:nigericin|FD-Score:-3.42|P-value:5.30E-5 ID:SGTC_958|Compound:1222-0007|FD-Score:4.33|P-value:3.63E-7 ID:SGTC_978|Compound:1416-0512|FD-Score:2.83|P-value:5.89E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.60|P-value:2.22E-5 ID:SGTC_1154|Compound:4088-0362|FD-Score:-2.81|P-value:7.42E-4 ID:SGTC_1156|Compound:4092-0367|FD-Score:-2.74|P-value:9.64E-4 ID:SGTC_1160|Compound:4092-0570|FD-Score:-3.22|P-value:1.33E-4 ID:SGTC_1164|Compound:k060-0068|FD-Score:-3.76|P-value:1.01E-5 ID:SGTC_1280|Compound:0880-0253|FD-Score:-2.78|P-value:8.34E-4 ID:SGTC_1321|Compound:1326-2144|FD-Score:2.76|P-value:7.79E-4 ID:SGTC_1567|Compound:st069303|FD-Score:3.06|P-value:2.26E-4 ID:SGTC_1603|Compound:st002045|FD-Score:3.29|P-value:8.35E-5 ID:SGTC_1693|Compound:xanthone|FD-Score:-2.84|P-value:6.69E-4 ID:SGTC_1703|Compound:st026983|FD-Score:3.16|P-value:1.44E-4 ID:SGTC_2024|Compound:5144227|FD-Score:-2.79|P-value:8.14E-4 ID:SGTC_2245|Compound:6637752|FD-Score:2.83|P-value:5.80E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-3.76|P-value:1.02E-5 ID:SGTC_2406|Compound:ethylparaben|FD-Score:-4.37|P-value:3.66E-7 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:2.87|P-value:5.04E-4 ID:SGTC_2486|Compound:5283202|FD-Score:-3.38|P-value:6.46E-5 ID:SGTC_2525|Compound:harmine|FD-Score:-2.73|P-value:9.96E-4 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:3.44|P-value:3.98E-5 ID:SGTC_2543|Compound:pimpinellin|FD-Score:-4.09|P-value:1.81E-6 ID:SGTC_2556|Compound:citropten|FD-Score:-4.06|P-value:2.04E-6 ID:SGTC_2568|Compound:diosmetin|FD-Score:2.96|P-value:3.43E-4 ID:SGTC_2789|Compound:5281112|FD-Score:3.19|P-value:1.26E-4 ID:SGTC_2917|Compound:7979103|FD-Score:3.34|P-value:6.51E-5 ID:SGTC_2925|Compound:9010536|FD-Score:2.86|P-value:5.13E-4 ID:SGTC_2991|Compound:9074669|FD-Score:3.11|P-value:1.79E-4 ID:SGTC_2992|Compound:9074591|FD-Score:-3.04|P-value:2.85E-4 ID:SGTC_3138|Compound:9097216|FD-Score:-3.26|P-value:1.10E-4 ID:SGTC_3193|Compound:9111931|FD-Score:-2.81|P-value:7.28E-4 ID:SGTC_3215|Compound:9129942|FD-Score:3.81|P-value:6.33E-6 ID:SGTC_3216|Compound:9129716|FD-Score:-3.53|P-value:3.12E-5 ID:SGTC_3219|Compound:9131112|FD-Score:3.65|P-value:1.46E-5 ID:SGTC_3328|Compound:9140624|FD-Score:4.56|P-value:9.34E-8 ID:SGTC_459|Compound:nigericin|FD-Score:-3.42|P-value:5.30E-5 ID:SGTC_958|Compound:1222-0007|FD-Score:4.33|P-value:3.63E-7 ID:SGTC_978|Compound:1416-0512|FD-Score:2.83|P-value:5.89E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.60|P-value:2.22E-5 ID:SGTC_1154|Compound:4088-0362|FD-Score:-2.81|P-value:7.42E-4 ID:SGTC_1156|Compound:4092-0367|FD-Score:-2.74|P-value:9.64E-4 ID:SGTC_1160|Compound:4092-0570|FD-Score:-3.22|P-value:1.33E-4 ID:SGTC_1164|Compound:k060-0068|FD-Score:-3.76|P-value:1.01E-5 ID:SGTC_1280|Compound:0880-0253|FD-Score:-2.78|P-value:8.34E-4 ID:SGTC_1321|Compound:1326-2144|FD-Score:2.76|P-value:7.79E-4 ID:SGTC_1567|Compound:st069303|FD-Score:3.06|P-value:2.26E-4 ID:SGTC_1603|Compound:st002045|FD-Score:3.29|P-value:8.35E-5 ID:SGTC_1693|Compound:xanthone|FD-Score:-2.84|P-value:6.69E-4 ID:SGTC_1703|Compound:st026983|FD-Score:3.16|P-value:1.44E-4 ID:SGTC_2024|Compound:5144227|FD-Score:-2.79|P-value:8.14E-4 ID:SGTC_2245|Compound:6637752|FD-Score:2.83|P-value:5.80E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-3.76|P-value:1.02E-5 ID:SGTC_2406|Compound:ethylparaben|FD-Score:-4.37|P-value:3.66E-7 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:2.87|P-value:5.04E-4 ID:SGTC_2486|Compound:5283202|FD-Score:-3.38|P-value:6.46E-5 ID:SGTC_2525|Compound:harmine|FD-Score:-2.73|P-value:9.96E-4 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:3.44|P-value:3.98E-5 ID:SGTC_2543|Compound:pimpinellin|FD-Score:-4.09|P-value:1.81E-6 ID:SGTC_2556|Compound:citropten|FD-Score:-4.06|P-value:2.04E-6 ID:SGTC_2568|Compound:diosmetin|FD-Score:2.96|P-value:3.43E-4 ID:SGTC_2789|Compound:5281112|FD-Score:3.19|P-value:1.26E-4 ID:SGTC_2917|Compound:7979103|FD-Score:3.34|P-value:6.51E-5 ID:SGTC_2925|Compound:9010536|FD-Score:2.86|P-value:5.13E-4 ID:SGTC_2991|Compound:9074669|FD-Score:3.11|P-value:1.79E-4 ID:SGTC_2992|Compound:9074591|FD-Score:-3.04|P-value:2.85E-4 ID:SGTC_3138|Compound:9097216|FD-Score:-3.26|P-value:1.10E-4 ID:SGTC_3193|Compound:9111931|FD-Score:-2.81|P-value:7.28E-4 ID:SGTC_3215|Compound:9129942|FD-Score:3.81|P-value:6.33E-6 ID:SGTC_3216|Compound:9129716|FD-Score:-3.53|P-value:3.12E-5 ID:SGTC_3219|Compound:9131112|FD-Score:3.65|P-value:1.46E-5 ID:SGTC_3328|Compound:9140624|FD-Score:4.56|P-value:9.34E-8

Top fitness defect scores for YER063W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3328 9140624 4.56 29.0 µM 9.34E-8 8 2.60E-6
2 SGTC_958 1222-0007 4.33 33.0 µM 3.63E-7 21 7.44E-6
3 SGTC_3215 9129942 3.81 49.5 µM 6.33E-6 11 6.84E-5
4 SGTC_3219 9131112 3.65 49.5 µM 1.46E-5 Golgi 43 1.31E-4
5 SGTC_2542 2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene 3.44 100.0 µM 3.98E-5 38 2.86E-4
6 SGTC_2917 7979103 3.34 53.8 µM 6.51E-5 53 4.19E-4
7 SGTC_1603 st002045 3.29 67.8 µM 8.35E-5 TSC3-RPN4 37 5.09E-4
8 SGTC_2789 5281112 3.19 71.4 µM 1.26E-4 74 6.99E-4
9 SGTC_1703 st026983 3.16 48.3 µM 1.44E-4 35 7.77E-4
10 SGTC_2991 9074669 3.11 71.4 µM 1.79E-4 azole & statin 113 9.21E-4
11 SGTC_1567 st069303 3.06 68.4 µM 2.26E-4 mitochondrial processes 63 0.00111
12 SGTC_2568 diosmetin 2.96 100.0 µM 3.43E-4 74 0.00153
13 SGTC_2420 aureobasidin a 2.87 50.0 nM 5.04E-4 108 0.00207
14 SGTC_2925 9010536 2.86 32.5 µM 5.13E-4 azole & statin 114 0.00209
15 SGTC_2245 6637752 2.83 200.0 µM 5.80E-4 47 0.00231
16 SGTC_978 1416-0512 2.83 87.4 µM 5.89E-4 redox potentiating 84 0.00234
17 SGTC_1321 1326-2144 2.76 215.0 µM 7.79E-4 59 0.00291
18 SGTC_2640 pentamidine 2.66 10.4 µM 0.00112 mitochondrial processes 111 0.00386
19 SGTC_1875 nocodazole 2.65 6.0 µM 0.00116 tubulin folding & SWR complex 108 0.00397
20 SGTC_1146 3579-1023 2.63 7.0 µM 0.00129 98 0.00432

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.162 5.37E-21 YPL249C GYP5 hom GTPase-activating protein (GAP) for yeast Rab family members; involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.150 2.78E-18 YKR031C SPO14 hom Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions
0.147 1.07E-17 YDR382W RPP2B hom Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm
0.117 9.08E-12 YLR422W_p YLR422W_p hom Protein of unknown function with similarity to human DOCK proteins (guanine nucleotide exchange factors); interacts with Ino4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, YLR422W is not an essential protein
0.115 2.25E-11 YML038C YMD8 hom Putative nucleotide sugar transporter, has similarity to Vrg4p
0.114 3.28E-11 YOR269W PAC1 hom Involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; serves at interface between dynein's ATPase site and its microtubule binding stalk, causing individual dynein motors to remain attached to microtubules for long periods; synthetic lethal with bni1; homolog of human LIS1, mutations in which cause the severe brain disorder lissencephaly
0.108 3.73E-10 YML003W_p YML003W_p hom Putative protein of unknown function
0.108 4.23E-10 YCR020C PET18 hom Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin
0.107 4.36E-10 YEL056W HAT2 hom Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing
0.107 5.98E-10 YBL018C POP8 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
0.104 1.81E-9 YHR006W STP2 hom Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication
0.101 4.67E-9 YMR079W SEC14 het Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs
0.098 1.21E-8 YFL041W FET5 hom Multicopper oxidase, integral membrane protein with similarity to Fet3p; may have a role in iron transport
0.096 2.91E-8 YFR022W ROG3 hom Protein that binds the ubiquitin ligase Rsp5p via its 2 PY motifs; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant; proposed to regulate the endocytosis of plasma membrane proteins; ROG3 has a paralog, ROD1, that arose from the whole genome duplication
0.095 3.58E-8 YDL197C ASF2 hom Anti-silencing protein that causes derepression of silent loci when overexpressed