HIPHOP chemogenomics database

YER069W / ARG5,6

Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine

Zygosity: Homozygous strain

fixedexpanded
Profile for YER069W / ARG5,6

Click on Significant Values for Screen Details

Top fitness defect scores for YER069W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_3211	9128781	15.30	49.5 µM	7.65E-63		1	3.05E-53
2	SGTC_2758	dehydroepiandrosterone	7.38	69.3 µM	4.73E-16		17	7.96E-14
3	SGTC_453	enantio-paf c16	6.17	10.0 µM	9.35E-12		40	3.39E-10
4	SGTC_530	4358-1714	4.89	28.0 µM	5.28E-8		19	5.07E-7
5	SGTC_54	0159-0055	4.77	9.0 µM	1.04E-7	ERG2	31	9.04E-7
6	SGTC_2998	9066762	4.34	71.4 µM	1.17E-6	RPP1 & pyrimidine depletion	20	7.05E-6
7	SGTC_392	N-ethylmaleimide	4.00	8.3 µM	7.06E-6	RNA pol III & RNase P/MRP	13	3.23E-5
8	SGTC_3135	9127742	3.91	49.5 µM	1.08E-5		24	4.63E-5
9	SGTC_1725	st036284	3.82	20.6 µM	1.62E-5		28	6.55E-5
10	SGTC_1630	st005209	3.58	31.3 µM	5.08E-5		36	1.73E-4
11	SGTC_1753	st043925	3.53	64.0 µM	6.40E-5		15	2.11E-4
12	SGTC_3123	9126162	3.39	49.5 µM	1.15E-4		33	3.48E-4
13	SGTC_676	falcarinol	3.30	140.0 µM	1.70E-4		80	4.85E-4
14	SGTC_3019	9081971	3.24	49.5 µM	2.17E-4		31	5.97E-4
15	SGTC_827	3253-0474	3.22	693.0 µM	2.33E-4		48	6.35E-4
16	SGTC_3143	9086658	2.91	49.5 µM	7.94E-4		113	0.00181
17	SGTC_1130	3454-2858	2.89	15.9 µM	8.51E-4	NEO1	145	0.00192
18	SGTC_1756	st041558	2.87	35.5 µM	9.04E-4		37	0.00202
19	SGTC_2542	2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene	2.80	100.0 µM	0.00118		76	0.00254
20	SGTC_1127	asiatic acid	2.80	681.4 nM	0.00120		97	0.00258

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.415	7.66E-140	YOL140W	ARG8	hom	Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine
0.254	1.30E-50	YGR122C-A_d	YGR122C-A_d	hom	Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W
0.226	4.76E-40	YJL088W	ARG3	hom	Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine
0.222	1.34E-38	YER068C-A_d	YER068C-A_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.217	6.46E-37	YKL151C	YKL151C	hom	NADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
0.216	7.61E-37	YBR265W	TSC10	het	3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family
0.215	1.77E-36	YOR130C	ORT1	hom	Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome
0.193	2.06E-29	YBR041W	FAT1	hom	Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids
0.187	1.15E-27	YJR148W	BAT2	hom	Cytosolic branched-chain amino acid (BCAA) aminotransferase, preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase
0.182	2.68E-26	YOL058W	ARG1	hom	Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate
0.168	1.40E-22	YBR062C_p	YBR062C_p	hom	Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway.
0.159	2.22E-20	YJL122W	ALB1	hom	Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p
0.158	3.77E-20	YJL071W	ARG2	hom	Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p
0.157	4.86E-20	YDR063W	AIM7	hom	Protein that interacts with Arp2/3 complex to stimulate actin filament debranching and inhibit actin nucleation; has similarity to Cof1p and also to human glia maturation factor (GMF); null mutant displays elevated mitochondrial genome loss
0.156	1.03E-19	YJL065C	DLS1	hom	Subunit of ISW2/yCHRAC chromatin accessibility complex; ISW2/yCHRAC also includes Itc1p, Isw2p, and Dpb4p; involved in inheritance of telomeric silencing; DLS1 has a paralog, DPB3, that arose from the whole genome duplication