YER091C / MET6

Cobalamin-independent methionine synthase, involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs

Zygosity: Homozygous strain
fixedexpanded
Profile for YER091C / MET6

Click on Significant Values for Screen Details ID:SGTC_70|Compound:0141-0289|FD-Score:-2.87|P-value:9.77E-4 ID:SGTC_113|Compound:0099-0221|FD-Score:3.02|P-value:1.50E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:6.22|P-value:1.99E-13 ID:SGTC_361|Compound:000s-0510|FD-Score:-3.46|P-value:8.53E-5 ID:SGTC_362|Compound:0310-0100|FD-Score:3.73|P-value:5.00E-6 ID:SGTC_364|Compound:0083-0097|FD-Score:2.63|P-value:7.50E-4 ID:SGTC_644|Compound:1498-1197|FD-Score:3.40|P-value:2.66E-5 ID:SGTC_646|Compound:4092-0839|FD-Score:3.04|P-value:1.41E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:2.69|P-value:6.14E-4 ID:SGTC_855|Compound:0428-0020|FD-Score:2.96|P-value:1.98E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:2.74|P-value:4.98E-4 ID:SGTC_950|Compound:1165-0508|FD-Score:-3.13|P-value:3.57E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:3.18|P-value:7.46E-5 ID:SGTC_980|Compound:1473-0395|FD-Score:-3.23|P-value:2.34E-4 ID:SGTC_1025|Compound:k007-0731|FD-Score:4.11|P-value:6.23E-7 ID:SGTC_1073|Compound:triclabendazole|FD-Score:5.93|P-value:2.12E-12 ID:SGTC_1119|Compound:parthenolide|FD-Score:3.18|P-value:7.28E-5 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:2.83|P-value:3.45E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:4.37|P-value:1.33E-7 ID:SGTC_1332|Compound:1480-0653|FD-Score:2.83|P-value:3.40E-4 ID:SGTC_1480|Compound:1070-0044|FD-Score:3.16|P-value:8.18E-5 ID:SGTC_1504|Compound:k297-0033|FD-Score:-4.40|P-value:6.81E-7 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.96|P-value:1.41E-6 ID:SGTC_1511|Compound:0906-3109|FD-Score:-2.90|P-value:9.02E-4 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:3.35|P-value:3.32E-5 ID:SGTC_1582|Compound:3',3',6'-trihydroxyflavone|FD-Score:2.58|P-value:9.14E-4 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:-3.00|P-value:6.08E-4 ID:SGTC_1655|Compound:st012546|FD-Score:2.63|P-value:7.69E-4 ID:SGTC_1740|Compound:st038286|FD-Score:2.61|P-value:8.21E-4 ID:SGTC_1750|Compound:st081859|FD-Score:3.05|P-value:1.32E-4 ID:SGTC_1752|Compound:st044819|FD-Score:3.22|P-value:6.04E-5 ID:SGTC_1830|Compound:st055982|FD-Score:3.52|P-value:1.45E-5 ID:SGTC_1832|Compound:st054113|FD-Score:2.71|P-value:5.60E-4 ID:SGTC_1868|Compound:st057644|FD-Score:3.25|P-value:5.34E-5 ID:SGTC_1895|Compound:st058434|FD-Score:2.74|P-value:4.94E-4 ID:SGTC_1902|Compound:st060212|FD-Score:-2.89|P-value:9.25E-4 ID:SGTC_2126|Compound:5251174|FD-Score:3.74|P-value:4.71E-6 ID:SGTC_2129|Compound:girgensonine|FD-Score:3.42|P-value:2.42E-5 ID:SGTC_2168|Compound:5727979|FD-Score:-2.88|P-value:9.49E-4 ID:SGTC_2171|Compound:5739187|FD-Score:-3.27|P-value:1.91E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:2.72|P-value:5.29E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:2.64|P-value:7.39E-4 ID:SGTC_2992|Compound:9074591|FD-Score:-3.10|P-value:3.98E-4 ID:SGTC_2999|Compound:9069765|FD-Score:3.10|P-value:1.08E-4 ID:SGTC_3287|Compound:9117173|FD-Score:4.03|P-value:9.65E-7 ID:SGTC_70|Compound:0141-0289|FD-Score:-2.87|P-value:9.77E-4 ID:SGTC_113|Compound:0099-0221|FD-Score:3.02|P-value:1.50E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:6.22|P-value:1.99E-13 ID:SGTC_361|Compound:000s-0510|FD-Score:-3.46|P-value:8.53E-5 ID:SGTC_362|Compound:0310-0100|FD-Score:3.73|P-value:5.00E-6 ID:SGTC_364|Compound:0083-0097|FD-Score:2.63|P-value:7.50E-4 ID:SGTC_644|Compound:1498-1197|FD-Score:3.40|P-value:2.66E-5 ID:SGTC_646|Compound:4092-0839|FD-Score:3.04|P-value:1.41E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:2.69|P-value:6.14E-4 ID:SGTC_855|Compound:0428-0020|FD-Score:2.96|P-value:1.98E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:2.74|P-value:4.98E-4 ID:SGTC_950|Compound:1165-0508|FD-Score:-3.13|P-value:3.57E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:3.18|P-value:7.46E-5 ID:SGTC_980|Compound:1473-0395|FD-Score:-3.23|P-value:2.34E-4 ID:SGTC_1025|Compound:k007-0731|FD-Score:4.11|P-value:6.23E-7 ID:SGTC_1073|Compound:triclabendazole|FD-Score:5.93|P-value:2.12E-12 ID:SGTC_1119|Compound:parthenolide|FD-Score:3.18|P-value:7.28E-5 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:2.83|P-value:3.45E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:4.37|P-value:1.33E-7 ID:SGTC_1332|Compound:1480-0653|FD-Score:2.83|P-value:3.40E-4 ID:SGTC_1480|Compound:1070-0044|FD-Score:3.16|P-value:8.18E-5 ID:SGTC_1504|Compound:k297-0033|FD-Score:-4.40|P-value:6.81E-7 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.96|P-value:1.41E-6 ID:SGTC_1511|Compound:0906-3109|FD-Score:-2.90|P-value:9.02E-4 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:3.35|P-value:3.32E-5 ID:SGTC_1582|Compound:3',3',6'-trihydroxyflavone|FD-Score:2.58|P-value:9.14E-4 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:-3.00|P-value:6.08E-4 ID:SGTC_1655|Compound:st012546|FD-Score:2.63|P-value:7.69E-4 ID:SGTC_1740|Compound:st038286|FD-Score:2.61|P-value:8.21E-4 ID:SGTC_1750|Compound:st081859|FD-Score:3.05|P-value:1.32E-4 ID:SGTC_1752|Compound:st044819|FD-Score:3.22|P-value:6.04E-5 ID:SGTC_1830|Compound:st055982|FD-Score:3.52|P-value:1.45E-5 ID:SGTC_1832|Compound:st054113|FD-Score:2.71|P-value:5.60E-4 ID:SGTC_1868|Compound:st057644|FD-Score:3.25|P-value:5.34E-5 ID:SGTC_1895|Compound:st058434|FD-Score:2.74|P-value:4.94E-4 ID:SGTC_1902|Compound:st060212|FD-Score:-2.89|P-value:9.25E-4 ID:SGTC_2126|Compound:5251174|FD-Score:3.74|P-value:4.71E-6 ID:SGTC_2129|Compound:girgensonine|FD-Score:3.42|P-value:2.42E-5 ID:SGTC_2168|Compound:5727979|FD-Score:-2.88|P-value:9.49E-4 ID:SGTC_2171|Compound:5739187|FD-Score:-3.27|P-value:1.91E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:2.72|P-value:5.29E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:2.64|P-value:7.39E-4 ID:SGTC_2992|Compound:9074591|FD-Score:-3.10|P-value:3.98E-4 ID:SGTC_2999|Compound:9069765|FD-Score:3.10|P-value:1.08E-4 ID:SGTC_3287|Compound:9117173|FD-Score:4.03|P-value:9.65E-7

Top fitness defect scores for YER091C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_245 methotrexate 6.22 289.6 µM 1.99E-13 11 2.47E-10
2 SGTC_1073 triclabendazole 5.93 3.1 µM 2.12E-12 7 1.49E-9
3 SGTC_1203 3992-3522 4.37 103.0 µM 1.33E-7 19 6.30E-6
4 SGTC_1025 k007-0731 4.11 70.0 µM 6.23E-7 10 2.02E-5
5 SGTC_3287 9117173 4.03 37.1 µM 9.65E-7 60S ribosome export 4 2.81E-5
6 SGTC_1510 1123-0200 3.96 71.5 µM 1.41E-6 42 3.74E-5
7 SGTC_2126 5251174 3.74 33.9 µM 4.71E-6 45 9.28E-5
8 SGTC_362 0310-0100 3.73 9.0 µM 5.00E-6 RPP1 & pyrimidine depletion 40 9.70E-5
9 SGTC_1830 st055982 3.52 50.3 µM 1.45E-5 35 2.16E-4
10 SGTC_2129 girgensonine 3.42 121.0 µM 2.42E-5 heme biosynthesis & mitochondrial translocase 57 3.18E-4
11 SGTC_644 1498-1197 3.40 63.3 µM 2.66E-5 46 3.42E-4
12 SGTC_1551 6-bromo-3'-methylflavone 3.35 63.5 µM 3.32E-5 41 4.03E-4
13 SGTC_1868 st057644 3.25 42.7 µM 5.34E-5 61 5.77E-4
14 SGTC_1752 st044819 3.22 83.0 µM 6.04E-5 48 6.33E-4
15 SGTC_1119 parthenolide 3.18 318.5 nM 7.28E-5 TSC3-RPN4 82 7.29E-4
16 SGTC_951 1171-0579 3.18 82.7 µM 7.46E-5 cell wall 125 7.42E-4
17 SGTC_1480 1070-0044 3.16 41.2 µM 8.18E-5 42 7.95E-4
18 SGTC_2999 9069765 3.10 71.4 µM 1.08E-4 amide catabolism 70 9.82E-4
19 SGTC_1750 st081859 3.05 2.4 µM 1.32E-4 endomembrane recycling 255 0.00114
20 SGTC_646 4092-0839 3.04 27.3 µM 1.41E-4 plasma membrane duress 59 0.00120

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.180 9.85E-26 YLL048C YBT1 hom Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters
0.145 3.42E-17 YJL125C GCD14 het Subunit of tRNA (1-methyladenosine) methyltransferase, with Gcd10p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
0.132 1.60E-14 YER056C FCY2 hom Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress
0.110 1.82E-10 YNL150W_d YNL150W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking
0.108 3.03E-10 YDR308C SRB7 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p
0.105 1.03E-9 YDR419W RAD30 hom DNA polymerase eta, involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; may also have a role in protection against mitochondrial mutagenesis; mutations in human pol eta are responsible for XPV
0.103 2.19E-9 YLR290C_p YLR290C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR290C is not an essential gene
0.102 3.40E-9 YDL115C IWR1 hom RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress
0.101 4.90E-9 YOR074C CDC21 het Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S
0.101 5.54E-9 YHR171W ATG7 hom Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation
0.093 5.84E-8 YNL011C_p YNL011C_p hom Putative protein of unknown function; YNL011C is not an essential gene
0.090 1.76E-7 YGR161C_p RTS3_p hom Putative component of the protein phosphatase type 2A complex
0.089 2.24E-7 YGL088W_d YGL088W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing
0.089 2.44E-7 YNL256W FOL1 het Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities
0.087 4.63E-7 YDR321W ASP1 hom Cytosolic L-asparaginase, involved in asparagine catabolism; catalyzes hydrolysis of L-asparagine to aspartic acid and ammonia, has an important role in therapy of acute lymphoblastic leukemia; synthesized constitutively