YER118C / SHO1

Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p

Zygosity: Homozygous strain
fixedexpanded
Profile for YER118C / SHO1

Click on Significant Values for Screen Details ID:SGTC_564|Compound:3555-0129|FD-Score:3.23|P-value:5.63E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:10.30|P-value:2.78E-25 ID:SGTC_1124|Compound:fisetin|FD-Score:4.55|P-value:2.32E-6 ID:SGTC_1142|Compound:3253-1408|FD-Score:3.18|P-value:6.65E-4 ID:SGTC_1145|Compound:3546-0597|FD-Score:-3.21|P-value:7.35E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:4.01|P-value:2.67E-5 ID:SGTC_1351|Compound:1496-0003|FD-Score:3.61|P-value:1.37E-4 ID:SGTC_1363|Compound:1636-0063|FD-Score:4.19|P-value:1.24E-5 ID:SGTC_1390|Compound:3413-2614|FD-Score:4.57|P-value:2.11E-6 ID:SGTC_1401|Compound:3807-4679|FD-Score:-3.99|P-value:3.69E-5 ID:SGTC_1549|Compound:4'-methoxyflavonol|FD-Score:-3.83|P-value:7.29E-5 ID:SGTC_1730|Compound:st033808|FD-Score:3.46|P-value:2.41E-4 ID:SGTC_1761|Compound:st044296|FD-Score:5.62|P-value:7.89E-9 ID:SGTC_1816|Compound:st051957|FD-Score:3.09|P-value:8.92E-4 ID:SGTC_1847|Compound:st056231|FD-Score:3.12|P-value:8.30E-4 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:-3.47|P-value:2.94E-4 ID:SGTC_2569|Compound:tetrandrine|FD-Score:-3.17|P-value:8.32E-4 ID:SGTC_2576|Compound:guaiol|FD-Score:-4.74|P-value:1.26E-6 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:-5.37|P-value:4.59E-8 ID:SGTC_2608|Compound:thymoquinone|FD-Score:-4.09|P-value:2.46E-5 ID:SGTC_2992|Compound:9074591|FD-Score:3.43|P-value:2.68E-4 ID:SGTC_3138|Compound:9097216|FD-Score:5.34|P-value:3.84E-8 ID:SGTC_3306|Compound:9127676|FD-Score:-3.16|P-value:8.63E-4 ID:SGTC_564|Compound:3555-0129|FD-Score:3.23|P-value:5.63E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:10.30|P-value:2.78E-25 ID:SGTC_1124|Compound:fisetin|FD-Score:4.55|P-value:2.32E-6 ID:SGTC_1142|Compound:3253-1408|FD-Score:3.18|P-value:6.65E-4 ID:SGTC_1145|Compound:3546-0597|FD-Score:-3.21|P-value:7.35E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:4.01|P-value:2.67E-5 ID:SGTC_1351|Compound:1496-0003|FD-Score:3.61|P-value:1.37E-4 ID:SGTC_1363|Compound:1636-0063|FD-Score:4.19|P-value:1.24E-5 ID:SGTC_1390|Compound:3413-2614|FD-Score:4.57|P-value:2.11E-6 ID:SGTC_1401|Compound:3807-4679|FD-Score:-3.99|P-value:3.69E-5 ID:SGTC_1549|Compound:4'-methoxyflavonol|FD-Score:-3.83|P-value:7.29E-5 ID:SGTC_1730|Compound:st033808|FD-Score:3.46|P-value:2.41E-4 ID:SGTC_1761|Compound:st044296|FD-Score:5.62|P-value:7.89E-9 ID:SGTC_1816|Compound:st051957|FD-Score:3.09|P-value:8.92E-4 ID:SGTC_1847|Compound:st056231|FD-Score:3.12|P-value:8.30E-4 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:-3.47|P-value:2.94E-4 ID:SGTC_2569|Compound:tetrandrine|FD-Score:-3.17|P-value:8.32E-4 ID:SGTC_2576|Compound:guaiol|FD-Score:-4.74|P-value:1.26E-6 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:-5.37|P-value:4.59E-8 ID:SGTC_2608|Compound:thymoquinone|FD-Score:-4.09|P-value:2.46E-5 ID:SGTC_2992|Compound:9074591|FD-Score:3.43|P-value:2.68E-4 ID:SGTC_3138|Compound:9097216|FD-Score:5.34|P-value:3.84E-8 ID:SGTC_3306|Compound:9127676|FD-Score:-3.16|P-value:8.63E-4

Top fitness defect scores for YER118C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_936 3005-4620 10.30 401.0 µM 2.78E-25 1 3.85E-25
2 SGTC_1761 st044296 5.62 37.0 µM 7.89E-9 3 9.42E-9
3 SGTC_3138 9097216 5.34 49.5 µM 3.84E-8 5 4.54E-8
4 SGTC_1390 3413-2614 4.57 145.0 µM 2.11E-6 fatty acid desaturase (OLE1) 12 2.43E-6
5 SGTC_1124 fisetin 4.55 1.2 µM 2.32E-6 20 2.67E-6
6 SGTC_1363 1636-0063 4.19 10.4 µM 1.24E-5 Golgi 22 1.42E-5
7 SGTC_1271 0832-2808 4.01 169.0 µM 2.67E-5 10 3.04E-5
8 SGTC_1351 1496-0003 3.61 46.5 µM 1.37E-4 26 1.54E-4
9 SGTC_1730 st033808 3.46 45.1 µM 2.41E-4 59 2.69E-4
10 SGTC_2992 9074591 3.43 71.4 µM 2.68E-4 26 3.00E-4
11 SGTC_564 3555-0129 3.23 330.0 µM 5.63E-4 21 6.25E-4
12 SGTC_1142 3253-1408 3.18 9.8 µM 6.65E-4 28 7.37E-4
13 SGTC_1847 st056231 3.12 15.0 µM 8.30E-4 78 9.20E-4
14 SGTC_1816 st051957 3.09 91.6 µM 8.92E-4 29 9.88E-4
15 SGTC_1694 st024011 3.01 44.9 µM 0.00118 ERG2 80 0.00130
16 SGTC_1923 st058458 2.91 19.1 µM 0.00165 102 0.00182
17 SGTC_360 0957-0399 2.86 1.0 µM 0.00194 cell wall 113 0.00213
18 SGTC_1674 st016616 2.86 23.6 µM 0.00194 80 0.00214
19 SGTC_1062 mesoridazine 2.83 14.2 µM 0.00209 72 0.00230
20 SGTC_41 0478-0632 2.83 82.6 µM 0.00212 92 0.00233

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.158 3.52E-20 YPL004C LSP1 hom Primary component of eisosomes, which are large immobile patch structures at the cell cortex associated with endocytosis, along with Pil1p and Sur7p; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family
0.118 7.74E-12 YLR049C_p YLR049C_p hom Putative protein of unknown function
0.114 3.58E-11 YLL016W SDC25 hom Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL017W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication
0.111 9.72E-11 YKL196C YKT6 het Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus
0.110 1.88E-10 YMR076C PDS5 het Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis
0.110 1.91E-10 YJL173C RFA3 het Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress
0.109 2.40E-10 YOL060C MAM3 hom Protein required for normal mitochondrial morphology, has similarity to hemolysins
0.109 2.48E-10 YFL034C-A RPL22B hom Ribosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication
0.105 1.06E-9 YLR285W NNT1 hom S-adenosylmethionine-dependent methyltransferase; has a role in rDNA silencing and in lifespan determination
0.105 1.23E-9 YBR044C TCM62 hom Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; putative chaperone
0.105 1.25E-9 YJL071W ARG2 hom Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p
0.102 2.95E-9 YOL151W GRE2 hom 3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase (D-lactaldehyde dehydrogenase); stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress
0.102 3.63E-9 YGL136C MRM2 hom Mitochondrial 2' O-ribose methyltransferase, required for methylation of U(2791) in 21S rRNA; MRM2 deletion confers thermosensitive respiration and loss of mitochondrial DNA; has similarity to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ
0.100 7.45E-9 YLR390W-A CCW14 hom Covalently linked cell wall glycoprotein, present in the inner layer of the cell wall
0.099 8.88E-9 YOR215C AIM41 hom Putative protein of unknown function; the authentic protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss