YER163C / GCG1

Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle

Zygosity: Homozygous strain
fixedexpanded
Profile for YER163C / GCG1

Click on Significant Values for Screen Details

Top fitness defect scores for YER163C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1893 st059353 7.58 36.1 µM 5.98E-15 1 1.68E-14
2 SGTC_2561 acetosyringone 6.44 100.0 µM 2.89E-11 3 6.07E-11
3 SGTC_2375 9072683 4.82 200.0 µM 4.76E-7 mitochondrial processes 9 7.20E-7
4 SGTC_2792 7535235 4.71 81.8 µM 8.25E-7 fatty acid desaturase (OLE1) 19 1.22E-6
5 SGTC_835 0312-0010 4.67 67.2 µM 1.01E-6 5 1.48E-6
6 SGTC_154 3389-1638 4.58 511.1 µM 1.62E-6 14 2.35E-6
7 SGTC_305 1682-2595 4.35 20.8 µM 4.78E-6 RNA processing & uracil transport 17 6.70E-6
8 SGTC_94 0334-0113 4.31 514.8 µM 5.74E-6 14 7.99E-6
9 SGTC_1423 4048-0112 4.28 5.6 µM 6.84E-6 20 9.47E-6
10 SGTC_1060 3448-7350 4.23 82.0 µM 8.42E-6 Golgi 28 1.16E-5
11 SGTC_2187 6125751 4.22 200.0 µM 8.75E-6 RSC complex & mRNA processing 11 1.20E-5
12 SGTC_1932 st074806 3.99 40.9 µM 2.45E-5 26 3.25E-5
13 SGTC_1927 st060202 3.98 13.8 µM 2.60E-5 17 3.44E-5
14 SGTC_34 0469-0683 3.93 81.8 µM 3.21E-5 SWF1 & branched chain AA biosynthesis 29 4.23E-5
15 SGTC_406 estrone 3.83 100.0 µM 5.00E-5 10 6.48E-5
16 SGTC_2993 9054257 3.83 71.4 µM 5.02E-5 fatty acid desaturase (OLE1) 50 6.51E-5
17 SGTC_2967 9089538 3.80 49.9 µM 5.58E-5 38 7.21E-5
18 SGTC_1826 st055655 3.78 41.6 µM 5.98E-5 ERG2 45 7.71E-5
19 SGTC_857 0438-0306 3.77 1.4 µM 6.24E-5 copper-dependent oxidative stress 16 8.03E-5
20 SGTC_1334 1486-0610 3.77 236.0 µM 6.45E-5 60S ribosome export 23 8.30E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.124 5.11E-13 YDL140C RPO21 het RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
0.089 2.76E-7 YNR014W_p YNR014W_p hom Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication
0.083 1.50E-6 YFR041C ERJ5 hom Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response
0.080 3.63E-6 YIR009W MSL1 hom U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members
0.079 4.60E-6 YLR349W_d YLR349W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified ORF DIC1/YLR348C
0.076 9.64E-6 YLR209C PNP1 hom Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway
0.075 1.22E-5 YPR097W YPR097W hom Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.075 1.37E-5 YLR455W YLR455W hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress
0.073 2.60E-5 YGL219C MDM34 hom Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth
0.072 2.75E-5 YBR220C_p YBR220C_p hom Putative protein of unknown function; YBR220C is not an essential gene
0.072 2.99E-5 YOL094C RFC4 het Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
0.072 3.10E-5 YNL332W THI12 hom Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13
0.072 3.16E-5 YDR179W-A_p YDR179W-A_p hom Putative protein of unknown function
0.071 3.71E-5 YDR246W TRS23 het One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4
0.071 4.20E-5 YPR182W SMX3 het Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F