YFR014C / CMK1

Calmodulin-dependent protein kinase; may play a role in stress response, many Ca++/calmodulin dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK1 has a paralog, CMK2, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YFR014C / CMK1

Click on Significant Values for Screen Details ID:SGTC_233|Compound:rapamycin|FD-Score:-3.28|P-value:1.05E-4 ID:SGTC_249|Compound:wiskostatin|FD-Score:3.86|P-value:6.77E-6 ID:SGTC_371|Compound:0195-0006|FD-Score:-2.93|P-value:4.57E-4 ID:SGTC_375|Compound:3970-0961|FD-Score:-2.84|P-value:6.64E-4 ID:SGTC_526|Compound:4469-0568|FD-Score:-4.49|P-value:1.94E-7 ID:SGTC_809|Compound:3998-0021|FD-Score:3.47|P-value:4.47E-5 ID:SGTC_818|Compound:4072-2707|FD-Score:-3.25|P-value:1.18E-4 ID:SGTC_820|Compound:1582-0060|FD-Score:-3.34|P-value:8.04E-5 ID:SGTC_827|Compound:3253-0474|FD-Score:2.93|P-value:4.75E-4 ID:SGTC_894|Compound:0960-0238|FD-Score:2.76|P-value:9.37E-4 ID:SGTC_914|Compound:3820-6779|FD-Score:-3.46|P-value:4.55E-5 ID:SGTC_918|Compound:glabranin|FD-Score:3.10|P-value:2.37E-4 ID:SGTC_1126|Compound:isoteolin|FD-Score:2.91|P-value:5.26E-4 ID:SGTC_1167|Compound:k781-0610|FD-Score:3.30|P-value:1.00E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:-3.27|P-value:1.11E-4 ID:SGTC_1372|Compound:2752-0194|FD-Score:-2.91|P-value:4.97E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.46|P-value:4.61E-5 ID:SGTC_1463|Compound:k081-0032|FD-Score:-3.22|P-value:1.36E-4 ID:SGTC_1638|Compound:st008370|FD-Score:-3.56|P-value:2.81E-5 ID:SGTC_1674|Compound:st016616|FD-Score:-3.11|P-value:2.18E-4 ID:SGTC_1675|Compound:st018485|FD-Score:3.02|P-value:3.24E-4 ID:SGTC_1975|Compound:st070967|FD-Score:3.25|P-value:1.25E-4 ID:SGTC_1976|Compound:st070609|FD-Score:4.35|P-value:4.69E-7 ID:SGTC_1984|Compound:st072354|FD-Score:-3.99|P-value:3.30E-6 ID:SGTC_1989|Compound:st071868|FD-Score:2.84|P-value:6.76E-4 ID:SGTC_2035|Compound:5154463|FD-Score:2.86|P-value:6.35E-4 ID:SGTC_2126|Compound:5251174|FD-Score:3.20|P-value:1.54E-4 ID:SGTC_2298|Compound:7976326|FD-Score:2.92|P-value:5.00E-4 ID:SGTC_2331|Compound:7980721|FD-Score:3.26|P-value:1.18E-4 ID:SGTC_2354|Compound:9060492|FD-Score:3.19|P-value:1.59E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-3.14|P-value:1.98E-4 ID:SGTC_2403|Compound:6032726|FD-Score:-3.05|P-value:2.84E-4 ID:SGTC_2589|Compound:lobaric acid|FD-Score:-5.30|P-value:1.06E-9 ID:SGTC_2616|Compound:abietic acid|FD-Score:3.77|P-value:1.07E-5 ID:SGTC_2694|Compound:st077685|FD-Score:-4.80|P-value:2.93E-8 ID:SGTC_2695|Compound:st077686|FD-Score:-2.89|P-value:5.39E-4 ID:SGTC_2794|Compound:7785382|FD-Score:3.49|P-value:4.18E-5 ID:SGTC_2887|Compound:9044784|FD-Score:-2.73|P-value:9.99E-4 ID:SGTC_2985|Compound:9022375|FD-Score:2.88|P-value:5.78E-4 ID:SGTC_3248|Compound:9135562|FD-Score:2.88|P-value:5.91E-4 ID:SGTC_233|Compound:rapamycin|FD-Score:-3.28|P-value:1.05E-4 ID:SGTC_249|Compound:wiskostatin|FD-Score:3.86|P-value:6.77E-6 ID:SGTC_371|Compound:0195-0006|FD-Score:-2.93|P-value:4.57E-4 ID:SGTC_375|Compound:3970-0961|FD-Score:-2.84|P-value:6.64E-4 ID:SGTC_526|Compound:4469-0568|FD-Score:-4.49|P-value:1.94E-7 ID:SGTC_809|Compound:3998-0021|FD-Score:3.47|P-value:4.47E-5 ID:SGTC_818|Compound:4072-2707|FD-Score:-3.25|P-value:1.18E-4 ID:SGTC_820|Compound:1582-0060|FD-Score:-3.34|P-value:8.04E-5 ID:SGTC_827|Compound:3253-0474|FD-Score:2.93|P-value:4.75E-4 ID:SGTC_894|Compound:0960-0238|FD-Score:2.76|P-value:9.37E-4 ID:SGTC_914|Compound:3820-6779|FD-Score:-3.46|P-value:4.55E-5 ID:SGTC_918|Compound:glabranin|FD-Score:3.10|P-value:2.37E-4 ID:SGTC_1126|Compound:isoteolin|FD-Score:2.91|P-value:5.26E-4 ID:SGTC_1167|Compound:k781-0610|FD-Score:3.30|P-value:1.00E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:-3.27|P-value:1.11E-4 ID:SGTC_1372|Compound:2752-0194|FD-Score:-2.91|P-value:4.97E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.46|P-value:4.61E-5 ID:SGTC_1463|Compound:k081-0032|FD-Score:-3.22|P-value:1.36E-4 ID:SGTC_1638|Compound:st008370|FD-Score:-3.56|P-value:2.81E-5 ID:SGTC_1674|Compound:st016616|FD-Score:-3.11|P-value:2.18E-4 ID:SGTC_1675|Compound:st018485|FD-Score:3.02|P-value:3.24E-4 ID:SGTC_1975|Compound:st070967|FD-Score:3.25|P-value:1.25E-4 ID:SGTC_1976|Compound:st070609|FD-Score:4.35|P-value:4.69E-7 ID:SGTC_1984|Compound:st072354|FD-Score:-3.99|P-value:3.30E-6 ID:SGTC_1989|Compound:st071868|FD-Score:2.84|P-value:6.76E-4 ID:SGTC_2035|Compound:5154463|FD-Score:2.86|P-value:6.35E-4 ID:SGTC_2126|Compound:5251174|FD-Score:3.20|P-value:1.54E-4 ID:SGTC_2298|Compound:7976326|FD-Score:2.92|P-value:5.00E-4 ID:SGTC_2331|Compound:7980721|FD-Score:3.26|P-value:1.18E-4 ID:SGTC_2354|Compound:9060492|FD-Score:3.19|P-value:1.59E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-3.14|P-value:1.98E-4 ID:SGTC_2403|Compound:6032726|FD-Score:-3.05|P-value:2.84E-4 ID:SGTC_2589|Compound:lobaric acid|FD-Score:-5.30|P-value:1.06E-9 ID:SGTC_2616|Compound:abietic acid|FD-Score:3.77|P-value:1.07E-5 ID:SGTC_2694|Compound:st077685|FD-Score:-4.80|P-value:2.93E-8 ID:SGTC_2695|Compound:st077686|FD-Score:-2.89|P-value:5.39E-4 ID:SGTC_2794|Compound:7785382|FD-Score:3.49|P-value:4.18E-5 ID:SGTC_2887|Compound:9044784|FD-Score:-2.73|P-value:9.99E-4 ID:SGTC_2985|Compound:9022375|FD-Score:2.88|P-value:5.78E-4 ID:SGTC_3248|Compound:9135562|FD-Score:2.88|P-value:5.91E-4

Top fitness defect scores for YFR014C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1976 st070609 4.35 63.5 µM 4.69E-7 6 6.88E-6
2 SGTC_249 wiskostatin 3.86 11.2 µM 6.77E-6 15 5.72E-5
3 SGTC_2616 abietic acid 3.77 100.0 µM 1.07E-5 37 8.21E-5
4 SGTC_2794 7785382 3.49 27.3 µM 4.18E-5 52 2.43E-4
5 SGTC_809 3998-0021 3.47 90.0 µM 4.47E-5 RNA processing & uracil transport 17 2.56E-4
6 SGTC_1167 k781-0610 3.30 148.0 µM 1.00E-4 RNA processing & uracil transport 51 4.88E-4
7 SGTC_2331 7980721 3.26 200.0 µM 1.18E-4 54 5.55E-4
8 SGTC_1975 st070967 3.25 30.8 µM 1.25E-4 65 5.81E-4
9 SGTC_2126 5251174 3.20 33.9 µM 1.54E-4 73 6.87E-4
10 SGTC_2354 9060492 3.19 162.0 µM 1.59E-4 47 7.03E-4
11 SGTC_918 glabranin 3.10 770.7 nM 2.37E-4 71 9.68E-4
12 SGTC_1675 st018485 3.02 24.5 µM 3.24E-4 60S ribosome export 73 0.00124
13 SGTC_827 3253-0474 2.93 693.0 µM 4.75E-4 67 0.00169
14 SGTC_2298 7976326 2.92 193.8 µM 5.00E-4 35 0.00176
15 SGTC_1126 isoteolin 2.91 1.0 µM 5.26E-4 87 0.00183
16 SGTC_2985 9022375 2.88 71.4 µM 5.78E-4 RPP1 & pyrimidine depletion 88 0.00198
17 SGTC_3248 9135562 2.88 49.5 µM 5.91E-4 39 0.00201
18 SGTC_2035 5154463 2.86 94.6 µM 6.35E-4 74 0.00213
19 SGTC_1989 st071868 2.84 50.2 µM 6.76E-4 57 0.00224
20 SGTC_894 0960-0238 2.76 55.9 µM 9.37E-4 55 0.00291

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.289 3.26E-65 YDR521W_d YDR521W_d hom Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.247 6.10E-48 YDR169C STB3 hom Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress
0.199 3.82E-31 YNL166C BNI5 hom Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.192 3.30E-29 YBL019W APN2 hom Class II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIII
0.186 1.55E-27 YGR194C XKS1 hom Xylulokinase, converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains
0.183 1.19E-26 YJR058C APS2 hom Small subunit of the clathrin-associated adaptor complex AP-2, which is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex
0.182 3.26E-26 YPR180W AOS1 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.180 6.32E-26 YJR146W_p YJR146W_p hom Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2
0.178 3.08E-25 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.175 1.61E-24 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.171 3.36E-23 YCL026C-A FRM2 hom Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin
0.169 1.05E-22 YDR312W SSF2 hom Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication
0.165 7.20E-22 YKR102W FLO10 hom Member of the FLO family of cell wall flocculation proteins; not expressed in most lab strains; overproduction induces flocculation that can be inhibited by mannose, sucrose, or glucose; overproduction also promotes haploid invasive growth and diploid filamentous growth
0.159 2.70E-20 YDR497C ITR1 hom Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress
0.157 7.98E-20 YLR179C YLR179C hom Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus