YFR021W / ATG18

Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood

Zygosity: Homozygous strain
fixedexpanded
Profile for YFR021W / ATG18

Click on Significant Values for Screen Details ID:SGTC_56|Compound:0957-0365|FD-Score:-3.77|P-value:1.19E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:3.38|P-value:4.98E-4 ID:SGTC_165|Compound:k038-0023|FD-Score:3.97|P-value:5.55E-5 ID:SGTC_216|Compound:k038-0035|FD-Score:3.39|P-value:4.91E-4 ID:SGTC_263|Compound:alizarin|FD-Score:4.11|P-value:3.13E-5 ID:SGTC_270|Compound:tamoxifen|FD-Score:7.77|P-value:1.88E-14 ID:SGTC_365|Compound:0121-0052|FD-Score:5.93|P-value:3.88E-9 ID:SGTC_369|Compound:0957-0393|FD-Score:3.37|P-value:5.22E-4 ID:SGTC_688|Compound:0527-0193|FD-Score:3.85|P-value:9.11E-5 ID:SGTC_993|Compound:3909-8757|FD-Score:4.14|P-value:2.82E-5 ID:SGTC_1127|Compound:asiatic acid|FD-Score:3.27|P-value:7.37E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.69|P-value:1.67E-4 ID:SGTC_1615|Compound:st002656|FD-Score:3.20|P-value:9.32E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:5.98|P-value:2.95E-9 ID:SGTC_1750|Compound:st081859|FD-Score:-3.25|P-value:7.56E-4 ID:SGTC_1769|Compound:st044406|FD-Score:3.69|P-value:1.65E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:3.33|P-value:6.05E-4 ID:SGTC_1860|Compound:st056228|FD-Score:4.20|P-value:2.18E-5 ID:SGTC_2055|Compound:5235184|FD-Score:3.66|P-value:1.82E-4 ID:SGTC_2064|Compound:5220758|FD-Score:3.25|P-value:7.74E-4 ID:SGTC_2299|Compound:7976368|FD-Score:4.33|P-value:1.25E-5 ID:SGTC_2403|Compound:6032726|FD-Score:-3.59|P-value:2.32E-4 ID:SGTC_2431|Compound:5490079|FD-Score:-3.35|P-value:5.49E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:3.19|P-value:9.63E-4 ID:SGTC_2841|Compound:9004761|FD-Score:3.28|P-value:7.13E-4 ID:SGTC_2871|Compound:9038510|FD-Score:-3.51|P-value:3.08E-4 ID:SGTC_2873|Compound:9040596|FD-Score:-3.32|P-value:6.11E-4 ID:SGTC_2899|Compound:9053889|FD-Score:4.08|P-value:3.64E-5 ID:SGTC_2909|Compound:7941780|FD-Score:5.61|P-value:2.39E-8 ID:SGTC_2914|Compound:7968218|FD-Score:4.51|P-value:5.70E-6 ID:SGTC_2955|Compound:9076254|FD-Score:3.43|P-value:4.18E-4 ID:SGTC_2956|Compound:9076196|FD-Score:-3.34|P-value:5.71E-4 ID:SGTC_3014|Compound:9081533|FD-Score:4.14|P-value:2.83E-5 ID:SGTC_3019|Compound:9081971|FD-Score:3.29|P-value:6.87E-4 ID:SGTC_3102|Compound:9121669|FD-Score:-3.65|P-value:1.86E-4 ID:SGTC_3112|Compound:9123419|FD-Score:-5.60|P-value:2.48E-8 ID:SGTC_3113|Compound:9123498|FD-Score:-3.24|P-value:8.03E-4 ID:SGTC_3114|Compound:9122766|FD-Score:5.50|P-value:4.41E-8 ID:SGTC_3119|Compound:9125183|FD-Score:3.28|P-value:7.07E-4 ID:SGTC_3274|Compound:9138298|FD-Score:-4.65|P-value:3.00E-6 ID:SGTC_3279|Compound:9140807|FD-Score:-3.36|P-value:5.25E-4 ID:SGTC_3326|Compound:9140064|FD-Score:4.57|P-value:4.24E-6 ID:SGTC_56|Compound:0957-0365|FD-Score:-3.77|P-value:1.19E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:3.38|P-value:4.98E-4 ID:SGTC_165|Compound:k038-0023|FD-Score:3.97|P-value:5.55E-5 ID:SGTC_216|Compound:k038-0035|FD-Score:3.39|P-value:4.91E-4 ID:SGTC_263|Compound:alizarin|FD-Score:4.11|P-value:3.13E-5 ID:SGTC_270|Compound:tamoxifen|FD-Score:7.77|P-value:1.88E-14 ID:SGTC_365|Compound:0121-0052|FD-Score:5.93|P-value:3.88E-9 ID:SGTC_369|Compound:0957-0393|FD-Score:3.37|P-value:5.22E-4 ID:SGTC_688|Compound:0527-0193|FD-Score:3.85|P-value:9.11E-5 ID:SGTC_993|Compound:3909-8757|FD-Score:4.14|P-value:2.82E-5 ID:SGTC_1127|Compound:asiatic acid|FD-Score:3.27|P-value:7.37E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.69|P-value:1.67E-4 ID:SGTC_1615|Compound:st002656|FD-Score:3.20|P-value:9.32E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:5.98|P-value:2.95E-9 ID:SGTC_1750|Compound:st081859|FD-Score:-3.25|P-value:7.56E-4 ID:SGTC_1769|Compound:st044406|FD-Score:3.69|P-value:1.65E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:3.33|P-value:6.05E-4 ID:SGTC_1860|Compound:st056228|FD-Score:4.20|P-value:2.18E-5 ID:SGTC_2055|Compound:5235184|FD-Score:3.66|P-value:1.82E-4 ID:SGTC_2064|Compound:5220758|FD-Score:3.25|P-value:7.74E-4 ID:SGTC_2299|Compound:7976368|FD-Score:4.33|P-value:1.25E-5 ID:SGTC_2403|Compound:6032726|FD-Score:-3.59|P-value:2.32E-4 ID:SGTC_2431|Compound:5490079|FD-Score:-3.35|P-value:5.49E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:3.19|P-value:9.63E-4 ID:SGTC_2841|Compound:9004761|FD-Score:3.28|P-value:7.13E-4 ID:SGTC_2871|Compound:9038510|FD-Score:-3.51|P-value:3.08E-4 ID:SGTC_2873|Compound:9040596|FD-Score:-3.32|P-value:6.11E-4 ID:SGTC_2899|Compound:9053889|FD-Score:4.08|P-value:3.64E-5 ID:SGTC_2909|Compound:7941780|FD-Score:5.61|P-value:2.39E-8 ID:SGTC_2914|Compound:7968218|FD-Score:4.51|P-value:5.70E-6 ID:SGTC_2955|Compound:9076254|FD-Score:3.43|P-value:4.18E-4 ID:SGTC_2956|Compound:9076196|FD-Score:-3.34|P-value:5.71E-4 ID:SGTC_3014|Compound:9081533|FD-Score:4.14|P-value:2.83E-5 ID:SGTC_3019|Compound:9081971|FD-Score:3.29|P-value:6.87E-4 ID:SGTC_3102|Compound:9121669|FD-Score:-3.65|P-value:1.86E-4 ID:SGTC_3112|Compound:9123419|FD-Score:-5.60|P-value:2.48E-8 ID:SGTC_3113|Compound:9123498|FD-Score:-3.24|P-value:8.03E-4 ID:SGTC_3114|Compound:9122766|FD-Score:5.50|P-value:4.41E-8 ID:SGTC_3119|Compound:9125183|FD-Score:3.28|P-value:7.07E-4 ID:SGTC_3274|Compound:9138298|FD-Score:-4.65|P-value:3.00E-6 ID:SGTC_3279|Compound:9140807|FD-Score:-3.36|P-value:5.25E-4 ID:SGTC_3326|Compound:9140064|FD-Score:4.57|P-value:4.24E-6

Top fitness defect scores for YFR021W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_270 tamoxifen 7.77 5.5 µM 1.88E-14 NEO1 3 3.79E-15
2 SGTC_1718 roemerine 5.98 11.9 µM 2.95E-9 redox potentiating 15 1.13E-9
3 SGTC_365 0121-0052 5.93 13.6 µM 3.88E-9 mitochondrial response to ROS 16 1.51E-9
4 SGTC_2909 7941780 5.61 10.5 µM 2.39E-8 4 1.02E-8
5 SGTC_3114 9122766 5.50 49.5 µM 4.41E-8 3 1.95E-8
6 SGTC_3326 9140064 4.57 16.4 µM 4.24E-6 13 2.39E-6
7 SGTC_2914 7968218 4.51 45.3 µM 5.70E-6 8 3.26E-6
8 SGTC_2299 7976368 4.33 114.9 µM 1.25E-5 21 7.43E-6
9 SGTC_1860 st056228 4.20 74.6 µM 2.18E-5 14 1.34E-5
10 SGTC_993 3909-8757 4.14 62.7 µM 2.82E-5 21 1.76E-5
11 SGTC_3014 9081533 4.14 49.5 µM 2.83E-5 9 1.76E-5
12 SGTC_263 alizarin 4.11 107.9 µM 3.13E-5 iron homeostasis 54 1.96E-5
13 SGTC_2899 9053889 4.08 58.4 µM 3.64E-5 7 2.29E-5
14 SGTC_165 k038-0023 3.97 64.4 µM 5.55E-5 ubiquinone biosynthesis & proteasome 74 3.57E-5
15 SGTC_688 0527-0193 3.85 473.0 µM 9.11E-5 fatty acid desaturase (OLE1) 15 6.02E-5
16 SGTC_1769 st044406 3.69 64.4 µM 1.65E-4 23 1.13E-4
17 SGTC_1263 0774-1208 3.69 64.8 µM 1.67E-4 55 1.14E-4
18 SGTC_2055 5235184 3.66 200.0 µM 1.82E-4 ERG2 46 1.25E-4
19 SGTC_2955 9076254 3.43 12.2 µM 4.18E-4 29 2.99E-4
20 SGTC_216 k038-0035 3.39 28.4 µM 4.91E-4 ubiquinone biosynthesis & proteasome 62 3.54E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.116 1.75E-11 YJR149W_p YJR149W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.109 2.06E-10 YDR376W ARH1 het Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability
0.105 9.34E-10 YJR150C DAN1 hom Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth
0.091 1.33E-7 YER073W ALD5 hom Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed
0.087 4.97E-7 YKL010C UFD4 hom Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress
0.085 8.52E-7 YHR042W NCP1 het NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p
0.081 2.54E-6 YPR014C_d YPR014C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene
0.078 6.84E-6 YDR292C SRP101 het Signal recognition particle (SRP) receptor alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with the beta subunit, Srp102p
0.077 8.07E-6 YLR273C PIG1 hom Putative targeting subunit for the type-1 protein phosphatase Glc7p that tethers it to the Gsy2p glycogen synthase
0.075 1.29E-5 YJR012C_p YJR012C_p het Essential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W
0.074 1.80E-5 YBR003W COQ1 hom Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis
0.073 2.12E-5 YPL252C YAH1 het Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
0.072 3.24E-5 YJL169W_d YJL169W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2
0.072 3.25E-5 YDL242W_d YDL242W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.070 4.94E-5 YGL111W NSA1 het Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis