YGL006W / PMC1

Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium; similar to mammalian PMCA1a

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL006W / PMC1

Click on Significant Values for Screen Details ID:SGTC_50|Compound:0929-0078|FD-Score:-3.72|P-value:3.77E-5 ID:SGTC_215|Compound:1287-0124|FD-Score:3.23|P-value:3.03E-4 ID:SGTC_764|Compound:1951-2361|FD-Score:3.72|P-value:3.87E-5 ID:SGTC_835|Compound:0312-0010|FD-Score:-3.54|P-value:8.38E-5 ID:SGTC_841|Compound:0251-0215|FD-Score:3.12|P-value:4.60E-4 ID:SGTC_1013|Compound:4112-3890|FD-Score:-3.48|P-value:1.06E-4 ID:SGTC_1128|Compound:3448-9350|FD-Score:3.29|P-value:2.35E-4 ID:SGTC_1277|Compound:0873-0036|FD-Score:2.94|P-value:9.02E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:3.42|P-value:1.40E-4 ID:SGTC_1342|Compound:1486-1464|FD-Score:2.94|P-value:8.98E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:3.02|P-value:6.80E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:2.98|P-value:7.74E-4 ID:SGTC_1619|Compound:st003326|FD-Score:2.93|P-value:9.46E-4 ID:SGTC_1750|Compound:st081859|FD-Score:3.03|P-value:6.45E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:3.57|P-value:7.36E-5 ID:SGTC_1955|Compound:wogonin|FD-Score:2.95|P-value:8.81E-4 ID:SGTC_2005|Compound:4024620|FD-Score:3.41|P-value:1.49E-4 ID:SGTC_2125|Compound:5246583|FD-Score:4.17|P-value:4.78E-6 ID:SGTC_2280|Compound:7785178|FD-Score:3.49|P-value:1.07E-4 ID:SGTC_2326|Compound:6164455|FD-Score:3.68|P-value:4.75E-5 ID:SGTC_2331|Compound:7980721|FD-Score:-3.45|P-value:1.18E-4 ID:SGTC_2426|Compound:5582046|FD-Score:-3.16|P-value:3.91E-4 ID:SGTC_2522|Compound:biochanin a|FD-Score:2.99|P-value:7.50E-4 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:2.92|P-value:9.75E-4 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:-3.51|P-value:9.24E-5 ID:SGTC_2672|Compound:12a-hydroxy-5-deoxydehydromunduserone|FD-Score:3.19|P-value:3.58E-4 ID:SGTC_2944|Compound:9067346|FD-Score:-3.41|P-value:1.44E-4 ID:SGTC_3007|Compound:9078383|FD-Score:3.68|P-value:4.57E-5 ID:SGTC_3016|Compound:9080866|FD-Score:-3.47|P-value:1.10E-4 ID:SGTC_3102|Compound:9121669|FD-Score:3.42|P-value:1.44E-4 ID:SGTC_3221|Compound:9128209|FD-Score:3.34|P-value:1.95E-4 ID:SGTC_3268|Compound:9137683|FD-Score:3.12|P-value:4.56E-4 ID:SGTC_50|Compound:0929-0078|FD-Score:-3.72|P-value:3.77E-5 ID:SGTC_215|Compound:1287-0124|FD-Score:3.23|P-value:3.03E-4 ID:SGTC_764|Compound:1951-2361|FD-Score:3.72|P-value:3.87E-5 ID:SGTC_835|Compound:0312-0010|FD-Score:-3.54|P-value:8.38E-5 ID:SGTC_841|Compound:0251-0215|FD-Score:3.12|P-value:4.60E-4 ID:SGTC_1013|Compound:4112-3890|FD-Score:-3.48|P-value:1.06E-4 ID:SGTC_1128|Compound:3448-9350|FD-Score:3.29|P-value:2.35E-4 ID:SGTC_1277|Compound:0873-0036|FD-Score:2.94|P-value:9.02E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:3.42|P-value:1.40E-4 ID:SGTC_1342|Compound:1486-1464|FD-Score:2.94|P-value:8.98E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:3.02|P-value:6.80E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:2.98|P-value:7.74E-4 ID:SGTC_1619|Compound:st003326|FD-Score:2.93|P-value:9.46E-4 ID:SGTC_1750|Compound:st081859|FD-Score:3.03|P-value:6.45E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:3.57|P-value:7.36E-5 ID:SGTC_1955|Compound:wogonin|FD-Score:2.95|P-value:8.81E-4 ID:SGTC_2005|Compound:4024620|FD-Score:3.41|P-value:1.49E-4 ID:SGTC_2125|Compound:5246583|FD-Score:4.17|P-value:4.78E-6 ID:SGTC_2280|Compound:7785178|FD-Score:3.49|P-value:1.07E-4 ID:SGTC_2326|Compound:6164455|FD-Score:3.68|P-value:4.75E-5 ID:SGTC_2331|Compound:7980721|FD-Score:-3.45|P-value:1.18E-4 ID:SGTC_2426|Compound:5582046|FD-Score:-3.16|P-value:3.91E-4 ID:SGTC_2522|Compound:biochanin a|FD-Score:2.99|P-value:7.50E-4 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:2.92|P-value:9.75E-4 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:-3.51|P-value:9.24E-5 ID:SGTC_2672|Compound:12a-hydroxy-5-deoxydehydromunduserone|FD-Score:3.19|P-value:3.58E-4 ID:SGTC_2944|Compound:9067346|FD-Score:-3.41|P-value:1.44E-4 ID:SGTC_3007|Compound:9078383|FD-Score:3.68|P-value:4.57E-5 ID:SGTC_3016|Compound:9080866|FD-Score:-3.47|P-value:1.10E-4 ID:SGTC_3102|Compound:9121669|FD-Score:3.42|P-value:1.44E-4 ID:SGTC_3221|Compound:9128209|FD-Score:3.34|P-value:1.95E-4 ID:SGTC_3268|Compound:9137683|FD-Score:3.12|P-value:4.56E-4

Top fitness defect scores for YGL006W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2125 5246583 4.17 200.0 µM 4.78E-6 tubulin folding & SWR complex 37 1.53E-5
2 SGTC_764 1951-2361 3.72 146.0 µM 3.87E-5 45 9.89E-5
3 SGTC_3007 9078383 3.68 71.4 µM 4.57E-5 24 1.14E-4
4 SGTC_2326 6164455 3.68 177.6 µM 4.75E-5 mitochondrial processes 30 1.19E-4
5 SGTC_1787 fluconazole 3.57 80.0 µM 7.36E-5 iron homeostasis 40 1.75E-4
6 SGTC_2280 7785178 3.49 200.0 µM 1.07E-4 18 2.45E-4
7 SGTC_1318 1280-3875 3.42 124.0 µM 1.40E-4 42 3.12E-4
8 SGTC_3102 9121669 3.42 49.5 µM 1.44E-4 16 3.18E-4
9 SGTC_2005 4024620 3.41 188.0 µM 1.49E-4 38 3.29E-4
10 SGTC_3221 9128209 3.34 49.5 µM 1.95E-4 29 4.19E-4
11 SGTC_1128 3448-9350 3.29 235.0 µM 2.35E-4 46 4.94E-4
12 SGTC_215 1287-0124 3.23 461.5 µM 3.03E-4 9 6.20E-4
13 SGTC_2672 12a-hydroxy-5-deoxydehydromunduserone 3.19 100.0 µM 3.58E-4 20 7.18E-4
14 SGTC_3268 9137683 3.12 49.5 µM 4.56E-4 27 8.93E-4
15 SGTC_841 0251-0215 3.12 91.2 µM 4.60E-4 109 9.00E-4
16 SGTC_1750 st081859 3.03 2.4 µM 6.45E-4 endomembrane recycling 257 0.00122
17 SGTC_1378 2093-0007 3.02 380.0 nM 6.80E-4 63 0.00128
18 SGTC_2522 biochanin a 2.99 4.9 µM 7.50E-4 RNA pol III & RNase P/MRP 41 0.00139
19 SGTC_1584 6-fluoro-dl-tryptophan 2.98 90.0 µM 7.74E-4 mitochondrial processes 61 0.00143
20 SGTC_1955 wogonin 2.95 32.4 µM 8.81E-4 61 0.00161

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.115 2.98E-11 YKL066W_d YKL066W_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene YNK1
0.105 1.28E-9 YBL014C RRN6 het Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
0.096 2.84E-8 YHR121W LSM12 hom Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress
0.096 3.07E-8 YGL263W COS12 hom Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
0.090 1.88E-7 YLL041C SDH2 hom Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain
0.087 5.04E-7 YGR086C PIL1 hom Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress
0.086 5.41E-7 YDL222C FMP45 hom Integral membrane protein localized to mitochondria (untagged protein); required for sporulation and maintaining sphingolipid content; has sequence similarity to SUR7 and YNL194C
0.083 1.41E-6 YBL081W_p YBL081W_p hom Non-essential protein of unknown function; null mutation results in a decrease in plasma membrane electron transport
0.082 2.01E-6 YNL162W-A_p YNL162W-A_p hom Putative protein of unknown function; identified by homology
0.081 2.61E-6 YGR199W PMT6 hom Protein O-mannosyltransferase, transfers mannose from dolichyl phosphate-D-mannose to protein serine/threonine residues of secretory proteins; reaction is essential for cell wall rigidity; member of a family of mannosyltransferases
0.080 4.00E-6 YER088C DOT6 hom Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.078 6.69E-6 YKL171W NNK1 hom Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm
0.077 7.08E-6 YKR067W GPT2 hom Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen
0.075 1.34E-5 YDR340W_d YDR340W_d hom Putative protein of unknown function
0.071 4.34E-5 YOL015W IRC10 hom Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci