YGL059W / PKP2

Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL059W / PKP2

Click on Significant Values for Screen Details ID:SGTC_44|Compound:0929-0055|FD-Score:4.82|P-value:6.20E-7 ID:SGTC_48|Compound:0382-0018|FD-Score:3.09|P-value:9.53E-4 ID:SGTC_61|Compound:0978-8327|FD-Score:-3.34|P-value:3.66E-4 ID:SGTC_254|Compound:nsc-64875|FD-Score:3.12|P-value:8.51E-4 ID:SGTC_273|Compound:hydroxyurea|FD-Score:-3.23|P-value:5.60E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:-3.97|P-value:3.11E-5 ID:SGTC_380|Compound:3970-0968|FD-Score:-4.91|P-value:3.67E-7 ID:SGTC_381|Compound:3461-2219|FD-Score:3.84|P-value:5.51E-5 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:4.10|P-value:1.83E-5 ID:SGTC_391|Compound:gliotoxin|FD-Score:6.01|P-value:7.38E-10 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:-3.99|P-value:2.83E-5 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:-3.29|P-value:4.44E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:-3.78|P-value:6.90E-5 ID:SGTC_512|Compound:prima-1|FD-Score:3.24|P-value:5.58E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:3.37|P-value:3.54E-4 ID:SGTC_790|Compound:1575-0012|FD-Score:3.86|P-value:5.11E-5 ID:SGTC_821|Compound:1114-0266|FD-Score:3.21|P-value:6.29E-4 ID:SGTC_957|Compound:1187-1561|FD-Score:3.17|P-value:7.24E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:4.28|P-value:8.25E-6 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.65|P-value:1.21E-4 ID:SGTC_1315|Compound:1237-0019|FD-Score:3.07|P-value:9.99E-4 ID:SGTC_1624|Compound:st003710|FD-Score:3.29|P-value:4.64E-4 ID:SGTC_1716|Compound:st033144|FD-Score:4.44|P-value:3.97E-6 ID:SGTC_1723|Compound:st034307|FD-Score:-3.66|P-value:1.11E-4 ID:SGTC_1753|Compound:st043925|FD-Score:3.12|P-value:8.64E-4 ID:SGTC_1756|Compound:st041558|FD-Score:3.28|P-value:4.88E-4 ID:SGTC_2070|Compound:5230947|FD-Score:-3.15|P-value:7.35E-4 ID:SGTC_2278|Compound:7948257|FD-Score:-3.45|P-value:2.48E-4 ID:SGTC_2305|Compound:7443522|FD-Score:3.82|P-value:6.05E-5 ID:SGTC_2456|Compound:5770359|FD-Score:-4.04|P-value:2.28E-5 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:3.45|P-value:2.60E-4 ID:SGTC_2765|Compound:myclobutanil|FD-Score:-4.03|P-value:2.41E-5 ID:SGTC_2770|Compound:toltrazuril|FD-Score:5.49|P-value:1.65E-8 ID:SGTC_2785|Compound:7756463|FD-Score:3.13|P-value:8.25E-4 ID:SGTC_2917|Compound:7979103|FD-Score:3.25|P-value:5.41E-4 ID:SGTC_2970|Compound:9090938|FD-Score:3.21|P-value:6.26E-4 ID:SGTC_3224|Compound:9130643|FD-Score:3.71|P-value:9.49E-5 ID:SGTC_44|Compound:0929-0055|FD-Score:4.82|P-value:6.20E-7 ID:SGTC_48|Compound:0382-0018|FD-Score:3.09|P-value:9.53E-4 ID:SGTC_61|Compound:0978-8327|FD-Score:-3.34|P-value:3.66E-4 ID:SGTC_254|Compound:nsc-64875|FD-Score:3.12|P-value:8.51E-4 ID:SGTC_273|Compound:hydroxyurea|FD-Score:-3.23|P-value:5.60E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:-3.97|P-value:3.11E-5 ID:SGTC_380|Compound:3970-0968|FD-Score:-4.91|P-value:3.67E-7 ID:SGTC_381|Compound:3461-2219|FD-Score:3.84|P-value:5.51E-5 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:4.10|P-value:1.83E-5 ID:SGTC_391|Compound:gliotoxin|FD-Score:6.01|P-value:7.38E-10 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:-3.99|P-value:2.83E-5 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:-3.29|P-value:4.44E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:-3.78|P-value:6.90E-5 ID:SGTC_512|Compound:prima-1|FD-Score:3.24|P-value:5.58E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:3.37|P-value:3.54E-4 ID:SGTC_790|Compound:1575-0012|FD-Score:3.86|P-value:5.11E-5 ID:SGTC_821|Compound:1114-0266|FD-Score:3.21|P-value:6.29E-4 ID:SGTC_957|Compound:1187-1561|FD-Score:3.17|P-value:7.24E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:4.28|P-value:8.25E-6 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.65|P-value:1.21E-4 ID:SGTC_1315|Compound:1237-0019|FD-Score:3.07|P-value:9.99E-4 ID:SGTC_1624|Compound:st003710|FD-Score:3.29|P-value:4.64E-4 ID:SGTC_1716|Compound:st033144|FD-Score:4.44|P-value:3.97E-6 ID:SGTC_1723|Compound:st034307|FD-Score:-3.66|P-value:1.11E-4 ID:SGTC_1753|Compound:st043925|FD-Score:3.12|P-value:8.64E-4 ID:SGTC_1756|Compound:st041558|FD-Score:3.28|P-value:4.88E-4 ID:SGTC_2070|Compound:5230947|FD-Score:-3.15|P-value:7.35E-4 ID:SGTC_2278|Compound:7948257|FD-Score:-3.45|P-value:2.48E-4 ID:SGTC_2305|Compound:7443522|FD-Score:3.82|P-value:6.05E-5 ID:SGTC_2456|Compound:5770359|FD-Score:-4.04|P-value:2.28E-5 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:3.45|P-value:2.60E-4 ID:SGTC_2765|Compound:myclobutanil|FD-Score:-4.03|P-value:2.41E-5 ID:SGTC_2770|Compound:toltrazuril|FD-Score:5.49|P-value:1.65E-8 ID:SGTC_2785|Compound:7756463|FD-Score:3.13|P-value:8.25E-4 ID:SGTC_2917|Compound:7979103|FD-Score:3.25|P-value:5.41E-4 ID:SGTC_2970|Compound:9090938|FD-Score:3.21|P-value:6.26E-4 ID:SGTC_3224|Compound:9130643|FD-Score:3.71|P-value:9.49E-5

Top fitness defect scores for YGL059W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_391 gliotoxin 6.01 8.3 µM 7.38E-10 21 9.45E-10
2 SGTC_2770 toltrazuril 5.49 17.5 µM 1.65E-8 6 2.02E-8
3 SGTC_44 0929-0055 4.82 61.2 µM 6.20E-7 20 7.23E-7
4 SGTC_1716 st033144 4.44 30.6 µM 3.97E-6 13 4.52E-6
5 SGTC_996 3970-0799 4.28 1.8 µM 8.25E-6 71 9.30E-6
6 SGTC_390 dec-rvkr-cmk 4.10 16.7 µM 1.83E-5 calcium & mitochondrial duress 19 2.04E-5
7 SGTC_790 1575-0012 3.86 79.1 µM 5.11E-5 TSC3-RPN4 32 5.62E-5
8 SGTC_381 3461-2219 3.84 42.7 µM 5.51E-5 copper-dependent oxidative stress 18 6.06E-5
9 SGTC_2305 7443522 3.82 69.2 µM 6.05E-5 18 6.64E-5
10 SGTC_3224 9130643 3.71 49.5 µM 9.49E-5 RPP1 & pyrimidine depletion 32 1.04E-4
11 SGTC_1125 tnp00248 3.65 1.3 µM 1.21E-4 41 1.31E-4
12 SGTC_2758 dehydroepiandrosterone 3.45 69.3 µM 2.60E-4 60 2.80E-4
13 SGTC_669 0108-0021 3.37 26.2 µM 3.54E-4 fatty acid desaturase (OLE1) 120 3.80E-4
14 SGTC_1624 st003710 3.29 6.2 µM 4.64E-4 calcium & mitochondrial duress 67 4.96E-4
15 SGTC_1756 st041558 3.28 35.5 µM 4.88E-4 23 5.22E-4
16 SGTC_2917 7979103 3.25 53.8 µM 5.41E-4 64 5.78E-4
17 SGTC_512 prima-1 3.24 270.0 µM 5.58E-4 DNA intercalators 46 5.96E-4
18 SGTC_2970 9090938 3.21 41.9 µM 6.26E-4 RNA processing & uracil transport 87 6.67E-4
19 SGTC_821 1114-0266 3.21 29.2 µM 6.29E-4 49 6.70E-4
20 SGTC_957 1187-1561 3.17 14.1 µM 7.24E-4 55 7.70E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.190 1.31E-28 YOR037W CYC2 hom Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
0.180 6.58E-26 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.159 1.52E-20 YGL222C EDC1 hom RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress
0.134 7.04E-15 YDL186W_p YDL186W_p hom Putative protein of unknown function; YDL186W is not an essential gene
0.133 1.12E-14 YDL099W BUG1 hom Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.122 1.15E-12 YGL040C HEM2 het Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
0.120 2.68E-12 YGL205W POX1 hom Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix
0.119 4.91E-12 YMR104C YPK2 hom Protein kinase with similarity to serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK; YPK2 has a paralog, YPK1, that arose from the whole genome duplication
0.119 5.21E-12 YER180C ISC10 hom Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells
0.118 5.66E-12 YJR094C IME1 hom Master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p
0.118 6.33E-12 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.117 1.15E-11 YPR126C_d YPR126C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.116 1.56E-11 YBR067C TIP1 hom Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
0.110 2.01E-10 YJR127C RSF2 hom Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.107 5.16E-10 YDR080W VPS41 hom Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport