YGL087C / MMS2

Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL087C / MMS2

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-3.40|P-value:2.00E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:4.18|P-value:7.26E-6 ID:SGTC_168|Compound:3970-0862|FD-Score:3.60|P-value:9.18E-5 ID:SGTC_214|Compound:4373-3496|FD-Score:3.52|P-value:1.27E-4 ID:SGTC_248|Compound:streptozotocin|FD-Score:7.01|P-value:1.68E-13 ID:SGTC_251|Compound:melphalan|FD-Score:3.14|P-value:5.52E-4 ID:SGTC_264|Compound:promethazine|FD-Score:5.02|P-value:9.36E-8 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:14.30|P-value:3.66E-50 ID:SGTC_429|Compound:1112-0049|FD-Score:11.70|P-value:2.49E-34 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.32|P-value:2.73E-4 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:3.42|P-value:1.96E-4 ID:SGTC_550|Compound:4123-0385|FD-Score:5.34|P-value:1.44E-8 ID:SGTC_567|Compound:1080-0542|FD-Score:3.51|P-value:1.37E-4 ID:SGTC_591|Compound:4299-0174|FD-Score:-3.09|P-value:6.38E-4 ID:SGTC_605|Compound:1315-0399|FD-Score:3.21|P-value:4.39E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:14.60|P-value:1.53E-52 ID:SGTC_935|Compound:2996-0153|FD-Score:4.02|P-value:1.53E-5 ID:SGTC_941|Compound:3237-0776|FD-Score:3.21|P-value:4.35E-4 ID:SGTC_950|Compound:1165-0508|FD-Score:-3.51|P-value:1.31E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:5.13|P-value:5.11E-8 ID:SGTC_1211|Compound:1056-0033|FD-Score:3.26|P-value:3.60E-4 ID:SGTC_1406|Compound:3909-7906|FD-Score:3.43|P-value:1.85E-4 ID:SGTC_1425|Compound:4048-4433|FD-Score:3.97|P-value:1.85E-5 ID:SGTC_1468|Compound:k284-1086|FD-Score:3.31|P-value:2.96E-4 ID:SGTC_1522|Compound:st012842|FD-Score:3.11|P-value:6.29E-4 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:3.12|P-value:6.05E-4 ID:SGTC_1758|Compound:st042942|FD-Score:3.14|P-value:5.58E-4 ID:SGTC_1855|Compound:st056195|FD-Score:5.11|P-value:5.57E-8 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:3.47|P-value:1.60E-4 ID:SGTC_1859|Compound:st055998|FD-Score:3.06|P-value:7.41E-4 ID:SGTC_1907|Compound:st060210|FD-Score:3.79|P-value:4.23E-5 ID:SGTC_1936|Compound:st074407|FD-Score:6.94|P-value:2.79E-13 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:-4.19|P-value:6.38E-6 ID:SGTC_2000|Compound:st075254|FD-Score:3.52|P-value:1.31E-4 ID:SGTC_2013|Compound:4024628|FD-Score:-3.00|P-value:8.99E-4 ID:SGTC_2066|Compound:5222451|FD-Score:3.02|P-value:8.60E-4 ID:SGTC_2305|Compound:7443522|FD-Score:-3.57|P-value:1.01E-4 ID:SGTC_2314|Compound:7727897|FD-Score:-4.90|P-value:1.71E-7 ID:SGTC_2347|Compound:9049783|FD-Score:3.98|P-value:1.76E-5 ID:SGTC_2535|Compound:4',4'-dimethoxydalbergione|FD-Score:3.67|P-value:6.94E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:4.26|P-value:4.82E-6 ID:SGTC_2619|Compound:obliquin|FD-Score:5.47|P-value:6.78E-9 ID:SGTC_2620|Compound:irigenin|FD-Score:4.55|P-value:1.14E-6 ID:SGTC_2677|Compound:mitomycin C|FD-Score:6.13|P-value:1.00E-10 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-3.85|P-value:3.06E-5 ID:SGTC_2689|Compound:tacrolimus|FD-Score:-3.62|P-value:8.12E-5 ID:SGTC_2753|Compound:fluvastatin|FD-Score:3.67|P-value:6.99E-5 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:-3.13|P-value:5.69E-4 ID:SGTC_2764|Compound:methyldopa|FD-Score:3.17|P-value:5.06E-4 ID:SGTC_2786|Compound:7743617|FD-Score:3.81|P-value:3.77E-5 ID:SGTC_2918|Compound:7977171|FD-Score:-3.42|P-value:1.89E-4 ID:SGTC_2985|Compound:9022375|FD-Score:4.95|P-value:1.35E-7 ID:SGTC_3017|Compound:9080681|FD-Score:3.90|P-value:2.60E-5 ID:SGTC_3205|Compound:9113483|FD-Score:3.53|P-value:1.22E-4 ID:SGTC_3217|Compound:9129673|FD-Score:3.44|P-value:1.80E-4 ID:SGTC_3254|Compound:9136651|FD-Score:4.27|P-value:4.54E-6 ID:SGTC_3258|Compound:9138477|FD-Score:-3.08|P-value:6.78E-4 ID:SGTC_3283|Compound:9109332|FD-Score:3.15|P-value:5.47E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.40|P-value:2.00E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:4.18|P-value:7.26E-6 ID:SGTC_168|Compound:3970-0862|FD-Score:3.60|P-value:9.18E-5 ID:SGTC_214|Compound:4373-3496|FD-Score:3.52|P-value:1.27E-4 ID:SGTC_248|Compound:streptozotocin|FD-Score:7.01|P-value:1.68E-13 ID:SGTC_251|Compound:melphalan|FD-Score:3.14|P-value:5.52E-4 ID:SGTC_264|Compound:promethazine|FD-Score:5.02|P-value:9.36E-8 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:14.30|P-value:3.66E-50 ID:SGTC_429|Compound:1112-0049|FD-Score:11.70|P-value:2.49E-34 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.32|P-value:2.73E-4 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:3.42|P-value:1.96E-4 ID:SGTC_550|Compound:4123-0385|FD-Score:5.34|P-value:1.44E-8 ID:SGTC_567|Compound:1080-0542|FD-Score:3.51|P-value:1.37E-4 ID:SGTC_591|Compound:4299-0174|FD-Score:-3.09|P-value:6.38E-4 ID:SGTC_605|Compound:1315-0399|FD-Score:3.21|P-value:4.39E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:14.60|P-value:1.53E-52 ID:SGTC_935|Compound:2996-0153|FD-Score:4.02|P-value:1.53E-5 ID:SGTC_941|Compound:3237-0776|FD-Score:3.21|P-value:4.35E-4 ID:SGTC_950|Compound:1165-0508|FD-Score:-3.51|P-value:1.31E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:5.13|P-value:5.11E-8 ID:SGTC_1211|Compound:1056-0033|FD-Score:3.26|P-value:3.60E-4 ID:SGTC_1406|Compound:3909-7906|FD-Score:3.43|P-value:1.85E-4 ID:SGTC_1425|Compound:4048-4433|FD-Score:3.97|P-value:1.85E-5 ID:SGTC_1468|Compound:k284-1086|FD-Score:3.31|P-value:2.96E-4 ID:SGTC_1522|Compound:st012842|FD-Score:3.11|P-value:6.29E-4 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:3.12|P-value:6.05E-4 ID:SGTC_1758|Compound:st042942|FD-Score:3.14|P-value:5.58E-4 ID:SGTC_1855|Compound:st056195|FD-Score:5.11|P-value:5.57E-8 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:3.47|P-value:1.60E-4 ID:SGTC_1859|Compound:st055998|FD-Score:3.06|P-value:7.41E-4 ID:SGTC_1907|Compound:st060210|FD-Score:3.79|P-value:4.23E-5 ID:SGTC_1936|Compound:st074407|FD-Score:6.94|P-value:2.79E-13 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:-4.19|P-value:6.38E-6 ID:SGTC_2000|Compound:st075254|FD-Score:3.52|P-value:1.31E-4 ID:SGTC_2013|Compound:4024628|FD-Score:-3.00|P-value:8.99E-4 ID:SGTC_2066|Compound:5222451|FD-Score:3.02|P-value:8.60E-4 ID:SGTC_2305|Compound:7443522|FD-Score:-3.57|P-value:1.01E-4 ID:SGTC_2314|Compound:7727897|FD-Score:-4.90|P-value:1.71E-7 ID:SGTC_2347|Compound:9049783|FD-Score:3.98|P-value:1.76E-5 ID:SGTC_2535|Compound:4',4'-dimethoxydalbergione|FD-Score:3.67|P-value:6.94E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:4.26|P-value:4.82E-6 ID:SGTC_2619|Compound:obliquin|FD-Score:5.47|P-value:6.78E-9 ID:SGTC_2620|Compound:irigenin|FD-Score:4.55|P-value:1.14E-6 ID:SGTC_2677|Compound:mitomycin C|FD-Score:6.13|P-value:1.00E-10 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-3.85|P-value:3.06E-5 ID:SGTC_2689|Compound:tacrolimus|FD-Score:-3.62|P-value:8.12E-5 ID:SGTC_2753|Compound:fluvastatin|FD-Score:3.67|P-value:6.99E-5 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:-3.13|P-value:5.69E-4 ID:SGTC_2764|Compound:methyldopa|FD-Score:3.17|P-value:5.06E-4 ID:SGTC_2786|Compound:7743617|FD-Score:3.81|P-value:3.77E-5 ID:SGTC_2918|Compound:7977171|FD-Score:-3.42|P-value:1.89E-4 ID:SGTC_2985|Compound:9022375|FD-Score:4.95|P-value:1.35E-7 ID:SGTC_3017|Compound:9080681|FD-Score:3.90|P-value:2.60E-5 ID:SGTC_3205|Compound:9113483|FD-Score:3.53|P-value:1.22E-4 ID:SGTC_3217|Compound:9129673|FD-Score:3.44|P-value:1.80E-4 ID:SGTC_3254|Compound:9136651|FD-Score:4.27|P-value:4.54E-6 ID:SGTC_3258|Compound:9138477|FD-Score:-3.08|P-value:6.78E-4 ID:SGTC_3283|Compound:9109332|FD-Score:3.15|P-value:5.47E-4

Top fitness defect scores for YGL087C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_915 methyl methanesulfonate (MMS) 14.60 110.0 mM 1.53E-52 DNA damage response 1 7.95E-49
2 SGTC_267 4-Nitroquinoline-1-oxide (4-NQO) 14.30 44.4 nM 3.66E-50 DNA damage response 8 1.27E-46
3 SGTC_429 1112-0049 11.70 25.5 µM 2.49E-34 DNA damage response 1 5.94E-32
4 SGTC_248 streptozotocin 7.01 5.2 mM 1.68E-13 DNA damage response 8 1.20E-12
5 SGTC_1936 st074407 6.94 74.3 µM 2.79E-13 DNA damage response 5 1.94E-12
6 SGTC_2677 mitomycin C 6.13 100.0 µM 1.00E-10 DNA damage response 5 4.53E-10
7 SGTC_2619 obliquin 5.47 87.9 µM 6.78E-9 cell wall 86 2.27E-8
8 SGTC_550 4123-0385 5.34 25.7 µM 1.44E-8 TSC3-RPN4 6 4.58E-8
9 SGTC_1060 3448-7350 5.13 82.0 µM 5.11E-8 Golgi 17 1.48E-7
10 SGTC_1855 st056195 5.11 39.6 µM 5.57E-8 60S ribosome export 4 1.61E-7
11 SGTC_264 promethazine 5.02 12.5 mM 9.36E-8 DNA intercalators 30 2.60E-7
12 SGTC_2985 9022375 4.95 71.4 µM 1.35E-7 RPP1 & pyrimidine depletion 14 3.66E-7
13 SGTC_2620 irigenin 4.55 100.0 µM 1.14E-6 18 2.65E-6
14 SGTC_3254 9136651 4.27 49.5 µM 4.54E-6 DNA damage response 17 9.58E-6
15 SGTC_2584 androsterone 4.26 48.7 µM 4.82E-6 cell wall 110 1.01E-5
16 SGTC_159 3970-0959 4.18 50.0 µM 7.26E-6 68 1.48E-5
17 SGTC_935 2996-0153 4.02 430.0 µM 1.53E-5 PDR1 19 2.96E-5
18 SGTC_2347 9049783 3.98 174.8 µM 1.76E-5 32 3.38E-5
19 SGTC_1425 4048-4433 3.97 7.3 µM 1.85E-5 copper-dependent oxidative stress 37 3.54E-5
20 SGTC_3017 9080681 3.90 49.5 µM 2.60E-5 15 4.85E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.548 1.55E-262 YDR092W UBC13 hom Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus
0.410 2.25E-136 YLR032W RAD5 hom DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
0.369 1.14E-108 YML095C RAD10 hom Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein
0.332 6.58E-87 YMR201C RAD14 hom Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein
0.309 2.08E-75 YGR258C RAD2 hom Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein
0.285 8.63E-64 YPL022W RAD1 hom Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein
0.284 4.28E-63 YER162C RAD4 hom Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins
0.274 5.82E-59 YCR066W RAD18 hom E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA
0.258 4.89E-52 YDR076W RAD55 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
0.244 1.23E-46 YDR004W RAD57 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
0.239 1.02E-44 YIR002C MPH1 hom 3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases
0.204 5.48E-33 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.196 1.70E-30 YEL037C RAD23 hom Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover
0.193 1.20E-29 YGL163C RAD54 hom DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress
0.171 2.47E-23 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p