YGL136C / MRM2

Mitochondrial 2' O-ribose methyltransferase, required for methylation of U(2791) in 21S rRNA; MRM2 deletion confers thermosensitive respiration and loss of mitochondrial DNA; has similarity to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL136C / MRM2

Click on Significant Values for Screen Details ID:SGTC_594|Compound:1487-1584|FD-Score:2.72|P-value:8.94E-4 ID:SGTC_762|Compound:1165-0429|FD-Score:2.98|P-value:3.05E-4 ID:SGTC_840|Compound:0245-0196|FD-Score:3.63|P-value:1.53E-5 ID:SGTC_861|Compound:0443-0273|FD-Score:3.39|P-value:4.98E-5 ID:SGTC_867|Compound:0518-0118|FD-Score:-3.10|P-value:1.72E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:-2.90|P-value:3.96E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:3.33|P-value:6.39E-5 ID:SGTC_923|Compound:1900-2434|FD-Score:2.86|P-value:5.19E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:3.64|P-value:1.47E-5 ID:SGTC_958|Compound:1222-0007|FD-Score:3.02|P-value:2.56E-4 ID:SGTC_1047|Compound:2043-3044|FD-Score:-3.66|P-value:1.15E-5 ID:SGTC_1112|Compound:tnp00069|FD-Score:2.82|P-value:5.91E-4 ID:SGTC_1196|Compound:1588-0294|FD-Score:3.76|P-value:7.63E-6 ID:SGTC_1202|Compound:1988-0068|FD-Score:3.30|P-value:7.40E-5 ID:SGTC_1208|Compound:2323-2347|FD-Score:3.78|P-value:7.08E-6 ID:SGTC_1297|Compound:1068-0111|FD-Score:2.95|P-value:3.44E-4 ID:SGTC_1319|Compound:1306-0155|FD-Score:3.46|P-value:3.48E-5 ID:SGTC_1516|Compound:st013856|FD-Score:4.30|P-value:3.84E-7 ID:SGTC_1734|Compound:st037168|FD-Score:3.23|P-value:1.03E-4 ID:SGTC_2003|Compound:4023581|FD-Score:-3.39|P-value:4.40E-5 ID:SGTC_2005|Compound:4024620|FD-Score:-3.77|P-value:6.60E-6 ID:SGTC_2282|Compound:7944268|FD-Score:5.56|P-value:8.05E-11 ID:SGTC_2290|Compound:7904846|FD-Score:2.92|P-value:3.97E-4 ID:SGTC_2313|Compound:7726885|FD-Score:3.12|P-value:1.66E-4 ID:SGTC_2503|Compound:beta-caryophyllene alcohol|FD-Score:3.75|P-value:8.31E-6 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.37|P-value:5.44E-5 ID:SGTC_2794|Compound:7785382|FD-Score:2.91|P-value:4.20E-4 ID:SGTC_2796|Compound:7949634|FD-Score:4.28|P-value:4.18E-7 ID:SGTC_2800|Compound:7887935|FD-Score:2.70|P-value:9.73E-4 ID:SGTC_2809|Compound:7964133|FD-Score:2.97|P-value:3.27E-4 ID:SGTC_2922|Compound:7999257|FD-Score:-2.70|P-value:9.11E-4 ID:SGTC_2923|Compound:7992654|FD-Score:-3.90|P-value:3.36E-6 ID:SGTC_3339|Compound:9145883|FD-Score:3.66|P-value:1.29E-5 ID:SGTC_594|Compound:1487-1584|FD-Score:2.72|P-value:8.94E-4 ID:SGTC_762|Compound:1165-0429|FD-Score:2.98|P-value:3.05E-4 ID:SGTC_840|Compound:0245-0196|FD-Score:3.63|P-value:1.53E-5 ID:SGTC_861|Compound:0443-0273|FD-Score:3.39|P-value:4.98E-5 ID:SGTC_867|Compound:0518-0118|FD-Score:-3.10|P-value:1.72E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:-2.90|P-value:3.96E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:3.33|P-value:6.39E-5 ID:SGTC_923|Compound:1900-2434|FD-Score:2.86|P-value:5.19E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:3.64|P-value:1.47E-5 ID:SGTC_958|Compound:1222-0007|FD-Score:3.02|P-value:2.56E-4 ID:SGTC_1047|Compound:2043-3044|FD-Score:-3.66|P-value:1.15E-5 ID:SGTC_1112|Compound:tnp00069|FD-Score:2.82|P-value:5.91E-4 ID:SGTC_1196|Compound:1588-0294|FD-Score:3.76|P-value:7.63E-6 ID:SGTC_1202|Compound:1988-0068|FD-Score:3.30|P-value:7.40E-5 ID:SGTC_1208|Compound:2323-2347|FD-Score:3.78|P-value:7.08E-6 ID:SGTC_1297|Compound:1068-0111|FD-Score:2.95|P-value:3.44E-4 ID:SGTC_1319|Compound:1306-0155|FD-Score:3.46|P-value:3.48E-5 ID:SGTC_1516|Compound:st013856|FD-Score:4.30|P-value:3.84E-7 ID:SGTC_1734|Compound:st037168|FD-Score:3.23|P-value:1.03E-4 ID:SGTC_2003|Compound:4023581|FD-Score:-3.39|P-value:4.40E-5 ID:SGTC_2005|Compound:4024620|FD-Score:-3.77|P-value:6.60E-6 ID:SGTC_2282|Compound:7944268|FD-Score:5.56|P-value:8.05E-11 ID:SGTC_2290|Compound:7904846|FD-Score:2.92|P-value:3.97E-4 ID:SGTC_2313|Compound:7726885|FD-Score:3.12|P-value:1.66E-4 ID:SGTC_2503|Compound:beta-caryophyllene alcohol|FD-Score:3.75|P-value:8.31E-6 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.37|P-value:5.44E-5 ID:SGTC_2794|Compound:7785382|FD-Score:2.91|P-value:4.20E-4 ID:SGTC_2796|Compound:7949634|FD-Score:4.28|P-value:4.18E-7 ID:SGTC_2800|Compound:7887935|FD-Score:2.70|P-value:9.73E-4 ID:SGTC_2809|Compound:7964133|FD-Score:2.97|P-value:3.27E-4 ID:SGTC_2922|Compound:7999257|FD-Score:-2.70|P-value:9.11E-4 ID:SGTC_2923|Compound:7992654|FD-Score:-3.90|P-value:3.36E-6 ID:SGTC_3339|Compound:9145883|FD-Score:3.66|P-value:1.29E-5

Top fitness defect scores for YGL136C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2282 7944268 5.56 200.0 µM 8.05E-11 RPP1 & pyrimidine depletion 7 1.36E-8
2 SGTC_1516 st013856 4.30 110.0 µM 3.84E-7 9 8.57E-6
3 SGTC_2796 7949634 4.28 45.5 µM 4.18E-7 12 9.14E-6
4 SGTC_1208 2323-2347 3.78 50.3 µM 7.08E-6 8 7.94E-5
5 SGTC_1196 1588-0294 3.76 184.0 µM 7.63E-6 18 8.41E-5
6 SGTC_2503 beta-caryophyllene alcohol 3.75 24.7 µM 8.31E-6 12 8.97E-5
7 SGTC_3339 9145883 3.66 14.9 µM 1.29E-5 18 1.25E-4
8 SGTC_936 3005-4620 3.64 401.0 µM 1.47E-5 33 1.39E-4
9 SGTC_840 0245-0196 3.63 390.0 µM 1.53E-5 18 1.43E-4
10 SGTC_1319 1306-0155 3.46 43.7 µM 3.48E-5 42 2.69E-4
11 SGTC_861 0443-0273 3.39 110.0 µM 4.98E-5 26 3.54E-4
12 SGTC_2629 bisabolol 3.37 100.0 µM 5.44E-5 42 3.79E-4
13 SGTC_908 3448-7491 3.33 47.7 µM 6.39E-5 mitochondrial stress 166 4.29E-4
14 SGTC_1202 1988-0068 3.30 29.6 µM 7.40E-5 RNA processing & uracil transport 53 4.79E-4
15 SGTC_1734 st037168 3.23 69.4 µM 1.03E-4 56 6.20E-4
16 SGTC_2313 7726885 3.12 200.0 µM 1.66E-4 RPP1 & pyrimidine depletion 65 8.91E-4
17 SGTC_958 1222-0007 3.02 33.0 µM 2.56E-4 72 0.00124
18 SGTC_762 1165-0429 2.98 115.0 µM 3.05E-4 TSC3-RPN4 65 0.00142
19 SGTC_2809 7964133 2.97 13.0 µM 3.27E-4 60S ribosome export 105 0.00150
20 SGTC_1297 1068-0111 2.95 14.1 µM 3.44E-4 42 0.00156

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.118 6.48E-12 YGR127W_p YGR127W_p hom Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response
0.116 1.42E-11 YBR083W TEC1 hom Transcription factor targeting filamentation genes and Ty1 expression; Ste12p activation of most filamentation gene promoters depends on Tec1p and Tec1p transcriptional activity is dependent on its association with Ste12p; binds to TCS elements upstream of filamentation genes, which are regulated by Tec1p/Ste12p/Dig1p complex; competes with Dig2p for binding to Ste12p/Dig1p; positive regulator of chronological life span; TEA/ATTS DNA-binding domain family member
0.104 1.31E-9 YER179W DMC1 hom Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p
0.102 3.63E-9 YER118C SHO1 hom Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p
0.099 8.63E-9 YGL142C GPI10 het Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog
0.099 9.39E-9 YLR387C REH1 hom Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains
0.095 3.52E-8 YPL003W ULA1 hom Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation
0.093 7.86E-8 YER068W MOT2 hom Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications
0.092 8.85E-8 YBR196C-B_p YBR196C-B_p hom Putative protein of unknown function; identified by expression profiling and mass spectrometry
0.086 6.06E-7 YNL181W_p YNL181W_p het Putative oxidoreductase; required for cell viability
0.086 6.04E-7 YNL146C-A_p YNL146C-A_p hom Putative protein of unknown function
0.086 6.90E-7 YIL121W QDR2 hom Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; exports copper; has broad substrate specificity and can transport many mono- and divalent cations; transports a variety of drugs and is required for resistance to quinidine, barban, cisplatin, and bleomycin; contributes to potassium homeostasis; expression is regulated by copper
0.085 8.21E-7 YJL015C_d YJL015C_d het Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W
0.084 1.01E-6 YBL102W SFT2 hom Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5
0.083 1.66E-6 YOL147C PEX11 hom Peroxisomal membrane protein required for medium-chain fatty acid oxidation and peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p