YGL141W / HUL5

Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL141W / HUL5

Click on Significant Values for Screen Details ID:SGTC_65|Compound:0798-0905|FD-Score:3.84|P-value:5.18E-5 ID:SGTC_71|Compound:0417-1750|FD-Score:-3.26|P-value:3.40E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:3.29|P-value:4.54E-4 ID:SGTC_174|Compound:3553-0389|FD-Score:-3.23|P-value:3.83E-4 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:3.17|P-value:6.86E-4 ID:SGTC_337|Compound:1315-0038|FD-Score:3.08|P-value:9.64E-4 ID:SGTC_592|Compound:k241-0073|FD-Score:-3.35|P-value:2.45E-4 ID:SGTC_765|Compound:1319-0117|FD-Score:-3.54|P-value:1.14E-4 ID:SGTC_771|Compound:0250-0039|FD-Score:-3.34|P-value:2.52E-4 ID:SGTC_849|Compound:0386-0493|FD-Score:3.09|P-value:9.10E-4 ID:SGTC_895|Compound:0964-0006|FD-Score:3.39|P-value:3.06E-4 ID:SGTC_899|Compound:1083-0020|FD-Score:4.16|P-value:1.25E-5 ID:SGTC_902|Compound:1120-0002|FD-Score:3.42|P-value:2.76E-4 ID:SGTC_1596|Compound:piperlongumine|FD-Score:3.16|P-value:7.27E-4 ID:SGTC_1734|Compound:st037168|FD-Score:-4.22|P-value:5.83E-6 ID:SGTC_1757|Compound:st041620|FD-Score:3.21|P-value:6.05E-4 ID:SGTC_2023|Compound:5144124|FD-Score:-3.70|P-value:5.87E-5 ID:SGTC_2054|Compound:5233173|FD-Score:3.67|P-value:1.02E-4 ID:SGTC_2225|Compound:6654152|FD-Score:3.13|P-value:7.99E-4 ID:SGTC_2356|Compound:9061756|FD-Score:-3.21|P-value:4.17E-4 ID:SGTC_2358|Compound:9037157|FD-Score:-3.71|P-value:5.65E-5 ID:SGTC_2359|Compound:9039622|FD-Score:-4.05|P-value:1.30E-5 ID:SGTC_2367|Compound:9070478|FD-Score:-3.43|P-value:1.75E-4 ID:SGTC_2386|Compound:9075165|FD-Score:-3.19|P-value:4.42E-4 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:3.28|P-value:4.70E-4 ID:SGTC_2610|Compound:ononetin|FD-Score:3.24|P-value:5.34E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:3.24|P-value:5.43E-4 ID:SGTC_2788|Compound:5282734|FD-Score:4.04|P-value:2.20E-5 ID:SGTC_2830|Compound:7992007|FD-Score:-4.48|P-value:1.71E-6 ID:SGTC_2834|Compound:9001755|FD-Score:4.09|P-value:1.69E-5 ID:SGTC_2836|Compound:9010338|FD-Score:3.58|P-value:1.47E-4 ID:SGTC_2844|Compound:9010103|FD-Score:-3.11|P-value:5.86E-4 ID:SGTC_2859|Compound:9028643|FD-Score:5.31|P-value:3.53E-8 ID:SGTC_3016|Compound:9080866|FD-Score:3.22|P-value:5.86E-4 ID:SGTC_3202|Compound:9113761|FD-Score:4.26|P-value:8.11E-6 ID:SGTC_3222|Compound:9128301|FD-Score:-3.12|P-value:5.60E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:3.84|P-value:5.18E-5 ID:SGTC_71|Compound:0417-1750|FD-Score:-3.26|P-value:3.40E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:3.29|P-value:4.54E-4 ID:SGTC_174|Compound:3553-0389|FD-Score:-3.23|P-value:3.83E-4 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:3.17|P-value:6.86E-4 ID:SGTC_337|Compound:1315-0038|FD-Score:3.08|P-value:9.64E-4 ID:SGTC_592|Compound:k241-0073|FD-Score:-3.35|P-value:2.45E-4 ID:SGTC_765|Compound:1319-0117|FD-Score:-3.54|P-value:1.14E-4 ID:SGTC_771|Compound:0250-0039|FD-Score:-3.34|P-value:2.52E-4 ID:SGTC_849|Compound:0386-0493|FD-Score:3.09|P-value:9.10E-4 ID:SGTC_895|Compound:0964-0006|FD-Score:3.39|P-value:3.06E-4 ID:SGTC_899|Compound:1083-0020|FD-Score:4.16|P-value:1.25E-5 ID:SGTC_902|Compound:1120-0002|FD-Score:3.42|P-value:2.76E-4 ID:SGTC_1596|Compound:piperlongumine|FD-Score:3.16|P-value:7.27E-4 ID:SGTC_1734|Compound:st037168|FD-Score:-4.22|P-value:5.83E-6 ID:SGTC_1757|Compound:st041620|FD-Score:3.21|P-value:6.05E-4 ID:SGTC_2023|Compound:5144124|FD-Score:-3.70|P-value:5.87E-5 ID:SGTC_2054|Compound:5233173|FD-Score:3.67|P-value:1.02E-4 ID:SGTC_2225|Compound:6654152|FD-Score:3.13|P-value:7.99E-4 ID:SGTC_2356|Compound:9061756|FD-Score:-3.21|P-value:4.17E-4 ID:SGTC_2358|Compound:9037157|FD-Score:-3.71|P-value:5.65E-5 ID:SGTC_2359|Compound:9039622|FD-Score:-4.05|P-value:1.30E-5 ID:SGTC_2367|Compound:9070478|FD-Score:-3.43|P-value:1.75E-4 ID:SGTC_2386|Compound:9075165|FD-Score:-3.19|P-value:4.42E-4 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:3.28|P-value:4.70E-4 ID:SGTC_2610|Compound:ononetin|FD-Score:3.24|P-value:5.34E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:3.24|P-value:5.43E-4 ID:SGTC_2788|Compound:5282734|FD-Score:4.04|P-value:2.20E-5 ID:SGTC_2830|Compound:7992007|FD-Score:-4.48|P-value:1.71E-6 ID:SGTC_2834|Compound:9001755|FD-Score:4.09|P-value:1.69E-5 ID:SGTC_2836|Compound:9010338|FD-Score:3.58|P-value:1.47E-4 ID:SGTC_2844|Compound:9010103|FD-Score:-3.11|P-value:5.86E-4 ID:SGTC_2859|Compound:9028643|FD-Score:5.31|P-value:3.53E-8 ID:SGTC_3016|Compound:9080866|FD-Score:3.22|P-value:5.86E-4 ID:SGTC_3202|Compound:9113761|FD-Score:4.26|P-value:8.11E-6 ID:SGTC_3222|Compound:9128301|FD-Score:-3.12|P-value:5.60E-4

Top fitness defect scores for YGL141W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2859 9028643 5.31 45.5 µM 3.53E-8 3 5.56E-8
2 SGTC_3202 9113761 4.26 49.5 µM 8.11E-6 37 1.04E-5
3 SGTC_899 1083-0020 4.16 478.0 µM 1.25E-5 19 1.57E-5
4 SGTC_2834 9001755 4.09 58.4 µM 1.69E-5 30 2.12E-5
5 SGTC_2788 5282734 4.04 71.4 µM 2.20E-5 43 2.72E-5
6 SGTC_65 0798-0905 3.84 35.0 µM 5.18E-5 calcium & mitochondrial duress 92 6.22E-5
7 SGTC_2054 5233173 3.67 129.0 µM 1.02E-4 mitochondrial response to ROS 65 1.19E-4
8 SGTC_2836 9010338 3.58 19.5 µM 1.47E-4 RPP1 & pyrimidine depletion 45 1.70E-4
9 SGTC_902 1120-0002 3.42 2.1 µM 2.76E-4 azole & statin 64 3.13E-4
10 SGTC_895 0964-0006 3.39 13.7 µM 3.06E-4 30 3.45E-4
11 SGTC_161 4065-0411 3.29 162.2 µM 4.54E-4 98 5.07E-4
12 SGTC_2588 indole-3-carbinol 3.28 100.0 µM 4.70E-4 80 5.23E-4
13 SGTC_2610 ononetin 3.24 44.2 µM 5.34E-4 76 5.93E-4
14 SGTC_2626 ethyl orsellinate 3.24 100.0 µM 5.43E-4 59 6.02E-4
15 SGTC_3016 9080866 3.22 49.5 µM 5.86E-4 51 6.49E-4
16 SGTC_1757 st041620 3.21 31.8 µM 6.05E-4 56 6.69E-4
17 SGTC_239 5hr heat shock (37°C) + mitomycin C 3.17 819.4 µM 6.86E-4 heat shock/prefoldin 63 7.55E-4
18 SGTC_1596 piperlongumine 3.16 63.0 µM 7.27E-4 42 7.99E-4
19 SGTC_2225 6654152 3.13 15.4 µM 7.99E-4 30 8.75E-4
20 SGTC_849 0386-0493 3.09 241.0 µM 9.10E-4 49 9.92E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.115 2.74E-11 YNL049C SFB2 hom Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb3p
0.111 1.23E-10 YDR043C NRG1 hom Transcriptional repressor; recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response; NRG1 has a paralog, NRG2, that arose from the whole genome duplication
0.105 1.08E-9 YJL187C SWE1 hom Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate
0.102 3.01E-9 YBR138C_p YBR138C_p hom Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene
0.098 1.23E-8 YIL158W AIM20 hom Putative protein of unknown function; overexpression causes a cell cycle delay or arrest; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.097 1.69E-8 YJL078C PRY3 hom Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p
0.092 1.01E-7 YGR217W CCH1 hom Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together
0.092 1.05E-7 YBR139W YBR139W hom Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner
0.088 2.82E-7 YNL104C LEU4 hom Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication
0.088 3.39E-7 YDR453C TSA2 hom Stress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants; protein abundance increases in response to DNA replication stress
0.088 3.45E-7 YLR080W EMP46 hom Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport
0.087 4.45E-7 YKR084C HBS1 hom GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay
0.086 5.91E-7 YBR169C SSE2 hom Member of the heat shock protein 70 (HSP70) family; may be involved in protein folding; localized to the cytoplasm; SSE2 has a paralog, SSE1, that arose from the whole genome duplication
0.086 6.32E-7 YOR337W TEA1 hom Ty1 enhancer activator required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein
0.085 8.30E-7 YLR124W_d YLR124W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data