HIPHOP chemogenomics database

YGL207W / SPT16

Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome

Zygosity: Heterozygous strain

fixedexpanded
Profile for YGL207W / SPT16

Click on Significant Values for Screen Details

Top fitness defect scores for YGL207W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_996	3970-0799	7.83	1.8 µM	2.49E-13		6	2.39E-15	1.12
2	SGTC_3131	9127911	6.17	49.5 µM	6.86E-9		1	3.49E-10	0.57
3	SGTC_1125	tnp00248	6.11	1.3 µM	9.18E-9		3	4.89E-10	0.56
4	SGTC_981	1488-1094	4.55	54.9 µM	1.52E-5		1	2.72E-6	0.56
5	SGTC_1778	st046819	4.49	58.9 µM	1.94E-5		4	3.61E-6	0.58
6	SGTC_1720	st037298	4.29	9.5 µM	4.25E-5		9	9.04E-6	0.21
7	SGTC_2153	5569715	4.22	200.0 µM	5.57E-5	iron homeostasis	3	1.24E-5	0.38
8	SGTC_2924	9010168	4.19	62.8 µM	6.07E-5	cell wall signaling	5	1.37E-5	0.30
9	SGTC_1725	st036284	4.18	20.6 µM	6.41E-5		5	1.46E-5	0.55
10	SGTC_1743	st037606	4.16	46.4 µM	6.90E-5		6	1.59E-5	0.34
11	SGTC_2359	9039622	4.08	200.0 µM	9.48E-5	endomembrane recycling	2	2.30E-5	0.06
12	SGTC_2805	7950783	4.02	58.4 µM	1.16E-4		8	2.90E-5	0.19
13	SGTC_1376	2910-0861	3.95	41.3 µM	1.51E-4		2	3.96E-5	0.29
14	SGTC_3316	9136639	3.82	10.6 µM	2.40E-4		6	6.77E-5	0.07
15	SGTC_3217	9129673	3.72	49.5 µM	3.38E-4		14	1.01E-4	0.14
16	SGTC_1278	0874-0585	3.66	172.0 µM	4.06E-4		10	1.25E-4	0.02
17	SGTC_2367	9070478	3.62	135.4 µM	4.68E-4	cell wall	12	1.47E-4	0.05
18	SGTC_1121	fusaric acid	3.57	630.5 nM	5.51E-4		4	1.78E-4	0.10
19	SGTC_1431	4049-0260	3.53	9.1 µM	6.31E-4		3	2.08E-4	0.29
20	SGTC_877	0771-0151	3.41	33.1 µM	9.18E-4		2	3.23E-4	0.04

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.277	3.27E-60	YML118W	NGL3	hom	3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.221	2.16E-38	YDL099W	BUG1	hom	Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.186	1.55E-27	YGL205W	POX1	hom	Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix
0.181	3.27E-26	YGL040C	HEM2	het	Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
0.174	3.22E-24	YDR505C	PSP1	hom	Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.171	2.51E-23	YBR067C	TIP1	hom	Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
0.169	7.09E-23	YDL186W_p	YDL186W_p	hom	Putative protein of unknown function; YDL186W is not an essential gene
0.161	5.28E-21	YGL222C	EDC1	hom	RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress
0.158	3.69E-20	YOR037W	CYC2	hom	Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
0.153	4.83E-19	YDL177C_p	YDL177C_p	hom	Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene
0.148	6.17E-18	YAL032C	PRP45	het	Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene
0.147	1.12E-17	YGR048W	UFD1	het	Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP)
0.146	1.46E-17	YGL096W	TOS8	hom	Homeodomain-containing protein and putative transcription factor; found associated with chromatin; target of SBF transcription factor; induced during meiosis and under cell-damaging conditions; TOS8 has a paralog, CUP9, that arose from the whole genome duplication
0.146	1.95E-17	YJL149W	DAS1	hom	Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C
0.144	5.96E-17	YJR094C	IME1	hom	Master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p