HIPHOP chemogenomics database

YGL218W_d

Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance

Zygosity: Homozygous strain

fixedexpanded
Profile for YGL218W / YGL218W

Click on Significant Values for Screen Details

Top fitness defect scores for YGL218W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1750	st081859	8.58	2.4 µM	7.19E-14	endomembrane recycling	57	4.84E-18
2	SGTC_236	1509-0011	7.35	5.3 µM	1.19E-10		261	9.62E-14
3	SGTC_2688	kanamycin b	6.05	96.7 µM	9.55E-8	calcium & mitochondrial duress	158	7.17E-10
4	SGTC_10	0279-0212	5.86	42.6 µM	2.29E-7	TRP & mitochondrial translation	31	2.29E-9
5	SGTC_684	1140-0066	5.63	328.0 µM	6.49E-7	Golgi	4	9.20E-9
6	SGTC_314	1671-0008	5.35	2.7 µM	2.08E-6		13	4.35E-8
7	SGTC_680	0417-1641	5.17	114.0 µM	4.46E-6	RPP1 & pyrimidine depletion	3	1.20E-7
8	SGTC_1883	5-benzylidenerhodanine	5.15	20.0 µM	4.79E-6	TRP & mitochondrial translation	27	1.31E-7
9	SGTC_28	pentachloropyridine N-oxide	5.04	1.6 µM	7.46E-6	TSC3-RPN4	28	2.37E-7
10	SGTC_3044	9093193	5.03	49.5 µM	7.67E-6		9	2.46E-7
11	SGTC_2804	7954240	4.83	19.5 µM	1.66E-5		14	6.89E-7
12	SGTC_1164	k060-0068	4.55	93.7 µM	4.60E-5		13	2.66E-6
13	SGTC_1742	st038235	4.44	28.9 µM	6.86E-5	mitochondrial stress	98	4.52E-6
14	SGTC_2540	epiandrosterone	4.42	100.0 µM	7.42E-5		44	5.02E-6
15	SGTC_1993	st073995	4.37	70.8 µM	8.77E-5		15	6.26E-6
16	SGTC_750	4073-0104	4.36	132.0 µM	9.18E-5		8	6.65E-6
17	SGTC_1075	aripiprazole	4.26	15.2 µM	1.26E-4		20	1.02E-5
18	SGTC_1519	esterone	4.21	74.0 µM	1.50E-4	DNA intercalators	14	1.27E-5
19	SGTC_68	0986-0284	4.13	82.8 µM	1.95E-4		63	1.80E-5
20	SGTC_275	trichlorophene	4.12	5.7 µM	2.04E-4	mitochondrial stress	220	1.91E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.325	2.30E-83	YMR228W	MTF1	hom	Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
0.319	7.33E-80	YGR215W	RSM27	hom	Mitochondrial ribosomal protein of the small subunit
0.316	1.63E-78	YER050C	RSM18	hom	Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein
0.315	4.21E-78	YDR194C	MSS116	hom	DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing
0.301	4.26E-71	YGR101W	PCP1	hom	Mitochondrial serine protease required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases
0.298	9.34E-70	YMR064W	AEP1	hom	Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase
0.297	2.43E-69	YDR268W	MSW1	hom	Mitochondrial tryptophanyl-tRNA synthetase
0.295	2.03E-68	YDR298C	ATP5	hom	Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated
0.293	1.70E-67	YER028C	MIG3	hom	Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication
0.291	2.89E-66	YPL183W-A	RTC6	hom	Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress
0.290	6.48E-66	YER087W	AIM10	hom	Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss
0.288	3.74E-65	YLR091W	GEP5	hom	Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine
0.288	5.67E-65	YLL033W	IRC19	hom	Putative protein of unknown function; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52p foci
0.288	7.50E-65	YLR149C	YLR149C	hom	Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress
0.283	1.21E-62	YHR168W	MTG2	hom	Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly