HIPHOP chemogenomics database

YGL251C / HFM1

Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity

Zygosity: Homozygous strain

fixedexpanded
Profile for YGL251C / HFM1

Click on Significant Values for Screen Details

Top fitness defect scores for YGL251C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_470	aa-861	5.86	153.0 µM	4.51E-8	sphingolipid biosynthesis & PDR1	24	2.28E-9
2	SGTC_955	1216-0110	5.69	33.0 µM	1.07E-7	sphingolipid biosynthesis & PDR1	34	6.37E-9
3	SGTC_2053	5265679	5.62	138.0 µM	1.48E-7		2	9.42E-9
4	SGTC_2630	antimycin a	5.42	100.0 µM	3.93E-7	mitochondrial processes	16	3.03E-8
5	SGTC_2457	5731572	4.92	11.7 µM	3.64E-6		8	4.33E-7
6	SGTC_960	1222-0186	4.86	7.7 µM	4.77E-6		15	5.98E-7
7	SGTC_749	1000-0129	4.61	128.0 µM	1.30E-5	amide catabolism	7	1.98E-6
8	SGTC_674	0498-0004	4.35	451.0 µM	3.68E-5	heme requiring	16	6.85E-6
9	SGTC_625	k213-0058	4.24	121.0 µM	5.55E-5	translation	86	1.12E-5
10	SGTC_750	4073-0104	4.11	132.0 µM	9.07E-5		10	2.01E-5
11	SGTC_2641	levomentholum	3.96	100.0 µM	1.51E-4		8	3.68E-5
12	SGTC_1893	st059353	3.96	36.1 µM	1.52E-4		25	3.71E-5
13	SGTC_2629	bisabolol	3.84	100.0 µM	2.30E-4		23	6.09E-5
14	SGTC_900	1112-0045	3.78	141.0 µM	2.86E-4	NEO1	27	7.87E-5
15	SGTC_2648	atorvastatin	3.76	78.7 µM	3.06E-4	azole & statin	24	8.52E-5
16	SGTC_2461	5755302	3.68	26.1 µM	3.97E-4		46	1.16E-4
17	SGTC_832	4083-0019	3.67	164.0 µM	4.06E-4	translation	117	1.19E-4
18	SGTC_2489	cantharidin	3.66	73.4 µM	4.20E-4	cell wall signaling	57	1.24E-4
19	SGTC_62	0180-0423	3.64	3.2 µM	4.54E-4	60S ribosome export	28	1.36E-4
20	SGTC_2332	7982454	3.58	138.9 µM	5.53E-4		68	1.72E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.226	5.62E-40	YDR516C	EMI2	hom	Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.204	5.38E-33	YPL208W	RKM1	hom	SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp)
0.170	4.47E-23	YIL039W	TED1	hom	Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
0.164	1.69E-21	YBR162C	TOS1	hom	Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C
0.157	4.92E-20	YOL100W	PKH2	hom	Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p
0.137	1.68E-15	YGL250W	RMR1	hom	Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus
0.133	1.08E-14	YGL236C	MTO1	hom	Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants
0.132	1.62E-14	YDR281C	PHM6	hom	Protein of unknown function, expression is regulated by phosphate levels
0.120	2.98E-12	YPL057C	SUR1	hom	Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication
0.119	4.23E-12	YGR114C_d	YGR114C_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
0.117	9.13E-12	YIL103W	DPH1	hom	Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p
0.115	2.83E-11	YDR140W	MTQ2	hom	S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC
0.113	5.20E-11	YDR072C	IPT1	hom	Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin
0.105	9.38E-10	YIL038C	NOT3	hom	Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation
0.103	2.57E-9	YBR036C	CSG2	hom	Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress