YGR023W / MTL1

Putative plasma membrane sensor, involved in cell integrity signaling and stress response during glucose starvation and oxidative stress; has structural and functional similarity to Mid2p

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR023W / MTL1

Click on Significant Values for Screen Details ID:SGTC_67|Compound:0335-0847|FD-Score:3.46|P-value:1.77E-4 ID:SGTC_74|Compound:000s-0487|FD-Score:-3.12|P-value:6.20E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-3.03|P-value:8.51E-4 ID:SGTC_281|Compound:1672-3440|FD-Score:3.06|P-value:7.73E-4 ID:SGTC_412|Compound:gemfibrozil|FD-Score:-3.18|P-value:5.08E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:-3.52|P-value:1.33E-4 ID:SGTC_611|Compound:3453-1652|FD-Score:3.89|P-value:2.85E-5 ID:SGTC_613|Compound:3914-0030|FD-Score:-3.05|P-value:8.09E-4 ID:SGTC_872|Compound:0588-0139|FD-Score:-3.53|P-value:1.31E-4 ID:SGTC_894|Compound:0960-0238|FD-Score:3.18|P-value:5.04E-4 ID:SGTC_912|Compound:3474-0017|FD-Score:3.15|P-value:5.64E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:3.83|P-value:3.82E-5 ID:SGTC_1238|Compound:0333-0287|FD-Score:3.06|P-value:7.91E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:3.65|P-value:8.17E-5 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-6.71|P-value:1.97E-12 ID:SGTC_1931|Compound:st074438|FD-Score:-3.57|P-value:1.11E-4 ID:SGTC_2057|Compound:5236571|FD-Score:5.26|P-value:2.71E-8 ID:SGTC_2109|Compound:5483026|FD-Score:3.44|P-value:1.91E-4 ID:SGTC_2118|Compound:5526350|FD-Score:4.29|P-value:4.57E-6 ID:SGTC_2238|Compound:6671620|FD-Score:-3.06|P-value:7.69E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-4.44|P-value:2.23E-6 ID:SGTC_2294|Compound:7971431|FD-Score:-4.40|P-value:2.71E-6 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:-6.97|P-value:2.76E-13 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:-3.37|P-value:2.45E-4 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:-3.54|P-value:1.25E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:-6.14|P-value:1.08E-10 ID:SGTC_2792|Compound:7535235|FD-Score:-3.11|P-value:6.56E-4 ID:SGTC_2802|Compound:7925815|FD-Score:-3.86|P-value:3.22E-5 ID:SGTC_2808|Compound:7946468|FD-Score:-3.25|P-value:3.89E-4 ID:SGTC_3016|Compound:9080866|FD-Score:3.12|P-value:6.38E-4 ID:SGTC_3078|Compound:9116413|FD-Score:-3.02|P-value:8.82E-4 ID:SGTC_3096|Compound:9117468|FD-Score:3.00|P-value:9.78E-4 ID:SGTC_3299|Compound:9121982|FD-Score:-3.76|P-value:5.05E-5 ID:SGTC_3305|Compound:9127027|FD-Score:-3.75|P-value:5.24E-5 ID:SGTC_3336|Compound:9143619|FD-Score:3.82|P-value:3.94E-5 ID:SGTC_67|Compound:0335-0847|FD-Score:3.46|P-value:1.77E-4 ID:SGTC_74|Compound:000s-0487|FD-Score:-3.12|P-value:6.20E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-3.03|P-value:8.51E-4 ID:SGTC_281|Compound:1672-3440|FD-Score:3.06|P-value:7.73E-4 ID:SGTC_412|Compound:gemfibrozil|FD-Score:-3.18|P-value:5.08E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:-3.52|P-value:1.33E-4 ID:SGTC_611|Compound:3453-1652|FD-Score:3.89|P-value:2.85E-5 ID:SGTC_613|Compound:3914-0030|FD-Score:-3.05|P-value:8.09E-4 ID:SGTC_872|Compound:0588-0139|FD-Score:-3.53|P-value:1.31E-4 ID:SGTC_894|Compound:0960-0238|FD-Score:3.18|P-value:5.04E-4 ID:SGTC_912|Compound:3474-0017|FD-Score:3.15|P-value:5.64E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:3.83|P-value:3.82E-5 ID:SGTC_1238|Compound:0333-0287|FD-Score:3.06|P-value:7.91E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:3.65|P-value:8.17E-5 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-6.71|P-value:1.97E-12 ID:SGTC_1931|Compound:st074438|FD-Score:-3.57|P-value:1.11E-4 ID:SGTC_2057|Compound:5236571|FD-Score:5.26|P-value:2.71E-8 ID:SGTC_2109|Compound:5483026|FD-Score:3.44|P-value:1.91E-4 ID:SGTC_2118|Compound:5526350|FD-Score:4.29|P-value:4.57E-6 ID:SGTC_2238|Compound:6671620|FD-Score:-3.06|P-value:7.69E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-4.44|P-value:2.23E-6 ID:SGTC_2294|Compound:7971431|FD-Score:-4.40|P-value:2.71E-6 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:-6.97|P-value:2.76E-13 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:-3.37|P-value:2.45E-4 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:-3.54|P-value:1.25E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:-6.14|P-value:1.08E-10 ID:SGTC_2792|Compound:7535235|FD-Score:-3.11|P-value:6.56E-4 ID:SGTC_2802|Compound:7925815|FD-Score:-3.86|P-value:3.22E-5 ID:SGTC_2808|Compound:7946468|FD-Score:-3.25|P-value:3.89E-4 ID:SGTC_3016|Compound:9080866|FD-Score:3.12|P-value:6.38E-4 ID:SGTC_3078|Compound:9116413|FD-Score:-3.02|P-value:8.82E-4 ID:SGTC_3096|Compound:9117468|FD-Score:3.00|P-value:9.78E-4 ID:SGTC_3299|Compound:9121982|FD-Score:-3.76|P-value:5.05E-5 ID:SGTC_3305|Compound:9127027|FD-Score:-3.75|P-value:5.24E-5 ID:SGTC_3336|Compound:9143619|FD-Score:3.82|P-value:3.94E-5

Top fitness defect scores for YGR023W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2057 5236571 5.26 38.3 µM 2.71E-8 RPP1 & pyrimidine depletion 9 7.19E-8
2 SGTC_2118 5526350 4.29 200.0 µM 4.57E-6 11 8.81E-6
3 SGTC_611 3453-1652 3.89 23.7 µM 2.85E-5 21 4.92E-5
4 SGTC_915 methyl methanesulfonate (MMS) 3.83 110.0 mM 3.82E-5 DNA damage response 50 6.48E-5
5 SGTC_3336 9143619 3.82 71.4 µM 3.94E-5 23 6.66E-5
6 SGTC_1787 fluconazole 3.65 80.0 µM 8.17E-5 iron homeostasis 35 1.32E-4
7 SGTC_67 0335-0847 3.46 266.0 µM 1.77E-4 ubiquinone biosynthesis & proteasome 147 2.73E-4
8 SGTC_2109 5483026 3.44 200.0 µM 1.91E-4 RSC complex & mRNA processing 47 2.93E-4
9 SGTC_894 0960-0238 3.18 55.9 µM 5.04E-4 31 7.31E-4
10 SGTC_912 3474-0017 3.15 2.2 µM 5.64E-4 53 8.12E-4
11 SGTC_3016 9080866 3.12 49.5 µM 6.38E-4 56 9.12E-4
12 SGTC_281 1672-3440 3.06 14.9 µM 7.73E-4 Golgi 29 0.00109
13 SGTC_1238 0333-0287 3.06 48.8 µM 7.91E-4 Golgi 64 0.00112
14 SGTC_3096 9117468 3.00 49.5 µM 9.78E-4 27 0.00136
15 SGTC_607 4491-0986 2.92 66.8 µM 0.00130 heme requiring 71 0.00178
16 SGTC_2798 7813637 2.88 45.5 µM 0.00147 92 0.00200
17 SGTC_2639 lactacystin 2.86 53.0 µM 0.00157 80 0.00213
18 SGTC_394 menadione 2.70 8.3 µM 0.00261 superoxide 101 0.00344
19 SGTC_763 3453-1109 2.68 128.0 µM 0.00278 85 0.00364
20 SGTC_2050 5263056 2.68 171.0 µM 0.00284 fatty acid desaturase (OLE1) 130 0.00372

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.134 8.40E-15 YDR242W AMD2 hom Putative amidase
0.121 1.84E-12 YKL117W SBA1 hom Co-chaperone that binds to and regulates Hsp90 family chaperones; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins, and like p23 can regulate telomerase activity; protein abundance increases in response to DNA replication stress
0.119 4.22E-12 YJL068C YJL068C hom Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D
0.119 5.21E-12 YDL012C YDL012C hom Tail-anchored plasma membrane protein with a conserved CYSTM module; possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype; YDL012C has a paralog, YBR016W, that arose from the whole genome duplication
0.116 1.51E-11 YGR097W ASK10 hom Component of RNA polymerase II holoenzyme; phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; proposed to function in activation of the glycerol channel Fps1p; ASK10 has a paralog, RGC1, that arose from the whole genome duplication
0.111 1.25E-10 YBR230C OM14 hom Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron
0.110 1.86E-10 YEL060C PRB1 hom Vacuolar proteinase B (yscB); serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress
0.109 3.03E-10 YGR017W_p YGR017W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm
0.108 3.89E-10 YMR110C HFD1 hom Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder
0.107 4.94E-10 YMR037C MSN2 hom Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN2 has a paralog, MSN4, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.107 5.64E-10 YGR174W-A_p YGR174W-A_p hom Putative protein of unknown function; predicted to have a role in cell budding based on computational guilt by association analysis
0.107 5.46E-10 YBL001C ECM15 hom Non-essential protein of unknown function, likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis
0.107 5.58E-10 YNL097C-B_p YNL097C-B_p hom Putative protein of unknown function
0.105 1.23E-9 YJL211C_d YJL211C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2
0.104 1.97E-9 YMR114C YMR114C hom Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene