HIPHOP chemogenomics database

YGR064W_d

Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C

Zygosity: Homozygous strain

fixedexpanded
Profile for YGR064W / YGR064W

Click on Significant Values for Screen Details

Top fitness defect scores for YGR064W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_562	3772-2733	10.30	112.0 µM	4.94E-17	ERG2	9	2.35E-25
2	SGTC_543	3844-1095	9.59	191.0 µM	7.26E-15	ERG2	4	4.58E-22
3	SGTC_2413	inermin	9.42	26.1 µM	2.07E-14		5	2.25E-21
4	SGTC_2417	cerulenin	8.77	830.0 nM	1.09E-12		2	9.23E-19
5	SGTC_1934	st074720	7.62	32.6 µM	5.68E-10		9	1.25E-14
6	SGTC_129	0088-0017	6.86	176.6 µM	2.33E-8		24	3.53E-12
7	SGTC_1304	1165-0502	6.77	251.0 µM	3.39E-8		23	6.26E-12
8	SGTC_109	0132-0036	6.74	33.7 µM	3.91E-8		6	7.78E-12
9	SGTC_2296	7971656	6.72	126.5 µM	4.25E-8		7	8.83E-12
10	SGTC_127	0180-0333	6.72	59.1 µM	4.42E-8	mitochondrial response to ROS	6	9.37E-12
11	SGTC_725	1497-0963	6.54	19.0 µM	9.47E-8	copper-dependent oxidative stress	4	2.99E-11
12	SGTC_2595	hexamethylquercetagetin	6.48	100.0 µM	1.24E-7		4	4.48E-11
13	SGTC_134	0269-0018	6.44	6.9 µM	1.46E-7		76	5.80E-11
14	SGTC_43	0570-0105	6.44	72.5 µM	1.50E-7	ERG2	27	6.03E-11
15	SGTC_2651	1327-0036	6.00	5.0 µM	9.47E-7		73	9.93E-10
16	SGTC_2932	9016711	5.70	44.5 µM	3.05E-6		15	5.89E-9
17	SGTC_453	enantio-paf c16	5.66	10.0 µM	3.63E-6		64	7.66E-9
18	SGTC_2618	avocadyne	5.62	43.4 µM	4.26E-6		21	9.77E-9
19	SGTC_3137	9097352	5.58	49.5 µM	4.83E-6		10	1.18E-8
20	SGTC_2814	7977482	5.42	43.0 µM	8.87E-6		12	2.98E-8

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.277	2.52E-60	YGR063C	SPT4	hom	Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions
0.276	9.67E-60	YOL051W	GAL11	hom	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator
0.252	8.80E-50	YGL025C	PGD1	hom	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
0.213	1.02E-35	YML062C	MFT1	hom	Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance
0.212	2.65E-35	YOL072W	THP1	hom	Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding
0.198	6.42E-31	YDR392W	SPT3	hom	Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
0.193	2.58E-29	YDR159W	SAC3	hom	Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP)
0.182	2.48E-26	YHR167W	THP2	hom	Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance
0.173	7.45E-24	YML122C_d	YML122C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.172	9.76E-24	YJR088C	EMC2	hom	Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35
0.155	1.62E-19	YMR015C	ERG5	hom	C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs
0.143	9.77E-17	YNL236W	SIN4	hom	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription
0.141	2.17E-16	YLR087C	CSF1	hom	Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.138	9.35E-16	YFL038C	YPT1	het	Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)
0.131	2.51E-14	YIR023W	DAL81	hom	Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism