HIPHOP chemogenomics database

YGR086C / PIL1

Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress

Zygosity: Homozygous strain

fixedexpanded
Profile for YGR086C / PIL1

Click on Significant Values for Screen Details

Top fitness defect scores for YGR086C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1853	st056710	8.41	91.2 µM	5.28E-19	amide catabolism	2	2.08E-17
2	SGTC_2688	kanamycin b	7.60	96.7 µM	7.09E-16	calcium & mitochondrial duress	83	1.46E-14
3	SGTC_382	3229-0966	5.53	23.2 µM	3.12E-9		2	1.62E-8
4	SGTC_1245	0418-0076	4.59	17.2 µM	7.05E-7	ERG2	17	2.25E-6
5	SGTC_2155	5570423	4.55	76.0 µM	8.79E-7	ergosterol biosynthesis	7	2.75E-6
6	SGTC_3037	9091026	4.27	49.5 µM	3.48E-6	NEO1-PIK1	106	9.61E-6
7	SGTC_3221	9128209	4.18	49.5 µM	5.58E-6		10	1.48E-5
8	SGTC_206	4546-0025	4.07	72.0 µM	9.30E-6	NEO1	64	2.35E-5
9	SGTC_1021	4476-0760	4.06	85.3 µM	9.87E-6		20	2.49E-5
10	SGTC_2131	toluylene red	3.92	5.3 µM	1.84E-5	60S ribosome export	25	4.38E-5
11	SGTC_3058	9093861	3.88	49.5 µM	2.23E-5		17	5.22E-5
12	SGTC_1715	st032160	3.88	10.2 µM	2.25E-5		45	5.27E-5
13	SGTC_682	0kpi-0099	3.85	17.0 µM	2.60E-5		76	6.01E-5
14	SGTC_2713	st079149	3.74	53.4 µM	4.14E-5		27	9.18E-5
15	SGTC_1716	st033144	3.72	30.6 µM	4.50E-5		27	9.89E-5
16	SGTC_1787	fluconazole	3.62	80.0 µM	6.94E-5	iron homeostasis	37	1.47E-4
17	SGTC_1155	4088-0394	3.58	48.2 µM	8.30E-5		44	1.73E-4
18	SGTC_1362	1611-0397	3.54	101.0 µM	9.91E-5		31	2.03E-4
19	SGTC_517	1365-0151	3.46	113.0 µM	1.33E-4		46	2.66E-4
20	SGTC_2967	9089538	3.46	49.9 µM	1.33E-4		52	2.67E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.176	1.24E-24	YDR101C	ARX1	hom	Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex
0.174	3.67E-24	YOR008C-A_p	YOR008C-A_p	hom	Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres
0.158	4.55E-20	YEL022W	GEA2	hom	Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication
0.142	1.54E-16	YIL002C	INP51	hom	Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth
0.130	4.44E-14	YBR287W_p	YBR287W_p	hom	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene
0.126	3.13E-13	YFL033C	RIM15	hom	Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
0.125	3.96E-13	YLR360W	VPS38	hom	Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated
0.123	9.68E-13	YKL061W_p	BLI1_p	hom	Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome
0.119	4.16E-12	YOR216C	RUD3	hom	Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1
0.118	6.08E-12	YLR443W	ECM7	hom	Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency
0.117	1.14E-11	YBL082C	ALG3	hom	Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins
0.117	1.23E-11	YJL053W	PEP8	hom	Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
0.116	1.39E-11	YDL052C	SLC1	hom	1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes
0.116	1.63E-11	YBR175W	SWD3	hom	Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5
0.115	2.80E-11	YDR456W	NHX1	hom	Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion