YGR144W / THI4

Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR144W / THI4

Click on Significant Values for Screen Details ID:SGTC_15|Compound:0266-0086|FD-Score:4.19|P-value:4.94E-5 ID:SGTC_115|Compound:0527-0192|FD-Score:3.72|P-value:2.75E-4 ID:SGTC_130|Compound:0136-0219|FD-Score:-3.89|P-value:1.27E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:4.40|P-value:2.16E-5 ID:SGTC_196|Compound:k015-0023|FD-Score:3.93|P-value:1.31E-4 ID:SGTC_535|Compound:2293-4129|FD-Score:3.86|P-value:1.65E-4 ID:SGTC_541|Compound:4513-1321|FD-Score:3.88|P-value:1.53E-4 ID:SGTC_581|Compound:0958-0271|FD-Score:3.74|P-value:2.57E-4 ID:SGTC_747|Compound:3448-8087|FD-Score:3.72|P-value:2.76E-4 ID:SGTC_872|Compound:0588-0139|FD-Score:4.02|P-value:9.36E-5 ID:SGTC_881|Compound:0833-0569|FD-Score:-3.42|P-value:6.45E-4 ID:SGTC_956|Compound:1185-0240|FD-Score:4.90|P-value:2.58E-6 ID:SGTC_994|Compound:3910-0338|FD-Score:-3.45|P-value:5.84E-4 ID:SGTC_1016|Compound:4226-1401|FD-Score:4.13|P-value:6.08E-5 ID:SGTC_1073|Compound:triclabendazole|FD-Score:3.91|P-value:1.39E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:-4.17|P-value:4.41E-5 ID:SGTC_1436|Compound:4092-1007|FD-Score:3.55|P-value:4.87E-4 ID:SGTC_1550|Compound:gossypolone|FD-Score:3.46|P-value:6.61E-4 ID:SGTC_1658|Compound:st012944|FD-Score:4.00|P-value:1.00E-4 ID:SGTC_1669|Compound:st015249|FD-Score:3.60|P-value:4.05E-4 ID:SGTC_1713|Compound:st033146|FD-Score:-4.67|P-value:5.88E-6 ID:SGTC_1718|Compound:roemerine|FD-Score:10.10|P-value:1.77E-21 ID:SGTC_1720|Compound:st037298|FD-Score:-3.80|P-value:1.73E-4 ID:SGTC_1721|Compound:st082152|FD-Score:8.85|P-value:8.03E-17 ID:SGTC_1724|Compound:st037281|FD-Score:5.27|P-value:4.73E-7 ID:SGTC_1749|Compound:st037932|FD-Score:5.17|P-value:7.43E-7 ID:SGTC_1754|Compound:st042065|FD-Score:3.88|P-value:1.56E-4 ID:SGTC_1772|Compound:st048439|FD-Score:-4.05|P-value:7.00E-5 ID:SGTC_1842|Compound:st055362|FD-Score:3.48|P-value:6.04E-4 ID:SGTC_2024|Compound:5144227|FD-Score:3.82|P-value:1.90E-4 ID:SGTC_2284|Compound:7945075|FD-Score:-7.93|P-value:5.41E-14 ID:SGTC_2285|Compound:7945142|FD-Score:4.81|P-value:3.83E-6 ID:SGTC_2405|Compound:ticlatone|FD-Score:4.61|P-value:8.86E-6 ID:SGTC_2514|Compound:salinomycin|FD-Score:-3.98|P-value:9.07E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.42|P-value:2.28E-7 ID:SGTC_2736|Compound:butenafine|FD-Score:-4.17|P-value:4.48E-5 ID:SGTC_2822|Compound:7994718|FD-Score:4.31|P-value:3.02E-5 ID:SGTC_2880|Compound:9042983|FD-Score:3.82|P-value:1.90E-4 ID:SGTC_2947|Compound:9072639|FD-Score:3.67|P-value:3.22E-4 ID:SGTC_2989|Compound:9073649|FD-Score:-4.26|P-value:3.12E-5 ID:SGTC_3048|Compound:9093155|FD-Score:-3.45|P-value:5.94E-4 ID:SGTC_3050|Compound:9093513|FD-Score:3.84|P-value:1.78E-4 ID:SGTC_3224|Compound:9130643|FD-Score:3.52|P-value:5.41E-4 ID:SGTC_15|Compound:0266-0086|FD-Score:4.19|P-value:4.94E-5 ID:SGTC_115|Compound:0527-0192|FD-Score:3.72|P-value:2.75E-4 ID:SGTC_130|Compound:0136-0219|FD-Score:-3.89|P-value:1.27E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:4.40|P-value:2.16E-5 ID:SGTC_196|Compound:k015-0023|FD-Score:3.93|P-value:1.31E-4 ID:SGTC_535|Compound:2293-4129|FD-Score:3.86|P-value:1.65E-4 ID:SGTC_541|Compound:4513-1321|FD-Score:3.88|P-value:1.53E-4 ID:SGTC_581|Compound:0958-0271|FD-Score:3.74|P-value:2.57E-4 ID:SGTC_747|Compound:3448-8087|FD-Score:3.72|P-value:2.76E-4 ID:SGTC_872|Compound:0588-0139|FD-Score:4.02|P-value:9.36E-5 ID:SGTC_881|Compound:0833-0569|FD-Score:-3.42|P-value:6.45E-4 ID:SGTC_956|Compound:1185-0240|FD-Score:4.90|P-value:2.58E-6 ID:SGTC_994|Compound:3910-0338|FD-Score:-3.45|P-value:5.84E-4 ID:SGTC_1016|Compound:4226-1401|FD-Score:4.13|P-value:6.08E-5 ID:SGTC_1073|Compound:triclabendazole|FD-Score:3.91|P-value:1.39E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:-4.17|P-value:4.41E-5 ID:SGTC_1436|Compound:4092-1007|FD-Score:3.55|P-value:4.87E-4 ID:SGTC_1550|Compound:gossypolone|FD-Score:3.46|P-value:6.61E-4 ID:SGTC_1658|Compound:st012944|FD-Score:4.00|P-value:1.00E-4 ID:SGTC_1669|Compound:st015249|FD-Score:3.60|P-value:4.05E-4 ID:SGTC_1713|Compound:st033146|FD-Score:-4.67|P-value:5.88E-6 ID:SGTC_1718|Compound:roemerine|FD-Score:10.10|P-value:1.77E-21 ID:SGTC_1720|Compound:st037298|FD-Score:-3.80|P-value:1.73E-4 ID:SGTC_1721|Compound:st082152|FD-Score:8.85|P-value:8.03E-17 ID:SGTC_1724|Compound:st037281|FD-Score:5.27|P-value:4.73E-7 ID:SGTC_1749|Compound:st037932|FD-Score:5.17|P-value:7.43E-7 ID:SGTC_1754|Compound:st042065|FD-Score:3.88|P-value:1.56E-4 ID:SGTC_1772|Compound:st048439|FD-Score:-4.05|P-value:7.00E-5 ID:SGTC_1842|Compound:st055362|FD-Score:3.48|P-value:6.04E-4 ID:SGTC_2024|Compound:5144227|FD-Score:3.82|P-value:1.90E-4 ID:SGTC_2284|Compound:7945075|FD-Score:-7.93|P-value:5.41E-14 ID:SGTC_2285|Compound:7945142|FD-Score:4.81|P-value:3.83E-6 ID:SGTC_2405|Compound:ticlatone|FD-Score:4.61|P-value:8.86E-6 ID:SGTC_2514|Compound:salinomycin|FD-Score:-3.98|P-value:9.07E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.42|P-value:2.28E-7 ID:SGTC_2736|Compound:butenafine|FD-Score:-4.17|P-value:4.48E-5 ID:SGTC_2822|Compound:7994718|FD-Score:4.31|P-value:3.02E-5 ID:SGTC_2880|Compound:9042983|FD-Score:3.82|P-value:1.90E-4 ID:SGTC_2947|Compound:9072639|FD-Score:3.67|P-value:3.22E-4 ID:SGTC_2989|Compound:9073649|FD-Score:-4.26|P-value:3.12E-5 ID:SGTC_3048|Compound:9093155|FD-Score:-3.45|P-value:5.94E-4 ID:SGTC_3050|Compound:9093513|FD-Score:3.84|P-value:1.78E-4 ID:SGTC_3224|Compound:9130643|FD-Score:3.52|P-value:5.41E-4

Top fitness defect scores for YGR144W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1718 roemerine 10.10 11.9 µM 1.77E-21 redox potentiating 1 2.11E-24
2 SGTC_1721 st082152 8.85 9.3 µM 8.03E-17 60S ribosome export 3 4.54E-19
3 SGTC_2582 agaric acid 5.42 100.0 µM 2.28E-7 redox potentiating 196 3.01E-8
4 SGTC_1724 st037281 5.27 8.3 µM 4.73E-7 14 6.94E-8
5 SGTC_1749 st037932 5.17 54.2 µM 7.43E-7 14 1.16E-7
6 SGTC_956 1185-0240 4.90 191.0 µM 2.58E-6 13 4.84E-7
7 SGTC_2285 7945142 4.81 200.0 µM 3.83E-6 7 7.59E-7
8 SGTC_2405 ticlatone 4.61 3.5 µM 8.86E-6 46 1.98E-6
9 SGTC_143 4032-1194 4.40 15.7 µM 2.16E-5 46 5.48E-6
10 SGTC_2822 7994718 4.31 71.4 µM 3.02E-5 12 8.03E-6
11 SGTC_15 0266-0086 4.19 27.1 µM 4.94E-5 endomembrane recycling 53 1.41E-5
12 SGTC_1016 4226-1401 4.13 38.0 µM 6.08E-5 RNA pol III & RNase P/MRP 17 1.79E-5
13 SGTC_872 0588-0139 4.02 234.0 µM 9.36E-5 32 2.93E-5
14 SGTC_1658 st012944 4.00 20.0 µM 1.00E-4 iron homeostasis 78 3.17E-5
15 SGTC_196 k015-0023 3.93 18.1 µM 1.31E-4 PDR1 21 4.32E-5
16 SGTC_1073 triclabendazole 3.91 3.1 µM 1.39E-4 27 4.61E-5
17 SGTC_541 4513-1321 3.88 94.0 µM 1.53E-4 20 5.13E-5
18 SGTC_1754 st042065 3.88 16.1 µM 1.56E-4 NEO1-PIK1 186 5.26E-5
19 SGTC_535 2293-4129 3.86 48.2 µM 1.65E-4 NEO1 63 5.59E-5
20 SGTC_3050 9093513 3.84 49.5 µM 1.78E-4 20 6.09E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.207 1.32E-33 YLR329W REC102 hom Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination
0.168 1.11E-22 YPL232W SSO1 hom Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p
0.164 9.96E-22 YHR042W NCP1 het NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p
0.163 1.67E-21 YDR376W ARH1 het Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability
0.157 4.91E-20 YDR520C_p URC2_p hom Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism
0.146 2.10E-17 YNL027W CRZ1 hom Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress
0.141 3.22E-16 YOR282W_d YOR282W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
0.140 4.66E-16 YER073W ALD5 hom Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed
0.139 5.18E-16 YOR149C SMP3 het Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure
0.133 1.01E-14 YDL237W AIM6 hom Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene
0.130 3.64E-14 YOR049C RSB1 hom Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane
0.125 4.21E-13 YJL169W_d YJL169W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2
0.122 1.21E-12 YGR178C PBP1 hom Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress
0.118 7.41E-12 YDR481C PHO8 hom Repressible vacuolar alkaline phosphatase; regulated by levels of Pi and by Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN
0.111 1.39E-10 YOR375C GDH1 hom NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication