YGR180C / RNR4

Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR180C / RNR4

Click on Significant Values for Screen Details ID:SGTC_53|Compound:0973-0023|FD-Score:-3.50|P-value:6.38E-4 ID:SGTC_100|Compound:0086-0128|FD-Score:3.81|P-value:3.37E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:-3.36|P-value:9.73E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:3.62|P-value:6.20E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:4.28|P-value:6.56E-5 ID:SGTC_173|Compound:1273-0061|FD-Score:3.67|P-value:5.25E-4 ID:SGTC_273|Compound:hydroxyurea|FD-Score:5.58|P-value:2.78E-7 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.48|P-value:6.72E-4 ID:SGTC_360|Compound:0957-0399|FD-Score:3.83|P-value:3.12E-4 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:3.62|P-value:6.23E-4 ID:SGTC_500|Compound:h-89|FD-Score:4.90|P-value:5.72E-6 ID:SGTC_547|Compound:1120-0019|FD-Score:-4.03|P-value:1.07E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-3.66|P-value:3.73E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:3.57|P-value:7.37E-4 ID:SGTC_582|Compound:0987-0078|FD-Score:3.99|P-value:1.85E-4 ID:SGTC_857|Compound:0438-0306|FD-Score:3.61|P-value:6.34E-4 ID:SGTC_1000|Compound:4048-0884|FD-Score:4.23|P-value:7.81E-5 ID:SGTC_1093|Compound:fluphenazine|FD-Score:5.13|P-value:2.19E-6 ID:SGTC_1473|Compound:k844-0725|FD-Score:3.83|P-value:3.17E-4 ID:SGTC_1508|Compound:1080-0625|FD-Score:3.77|P-value:3.81E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:3.73|P-value:4.42E-4 ID:SGTC_1629|Compound:st005085|FD-Score:4.77|P-value:9.80E-6 ID:SGTC_1688|Compound:st021091|FD-Score:3.78|P-value:3.66E-4 ID:SGTC_1723|Compound:st034307|FD-Score:4.26|P-value:6.98E-5 ID:SGTC_1834|Compound:st055981|FD-Score:4.70|P-value:1.30E-5 ID:SGTC_1884|Compound:5633444|FD-Score:5.68|P-value:1.78E-7 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:3.96|P-value:2.05E-4 ID:SGTC_2309|Compound:7664088|FD-Score:-3.57|P-value:4.99E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:3.89|P-value:2.59E-4 ID:SGTC_2750|Compound:bromebric acid|FD-Score:5.72|P-value:1.40E-7 ID:SGTC_2754|Compound:granisetron|FD-Score:-4.08|P-value:8.93E-5 ID:SGTC_2836|Compound:9010338|FD-Score:3.78|P-value:3.77E-4 ID:SGTC_2881|Compound:9058953|FD-Score:3.62|P-value:6.14E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-4.83|P-value:4.72E-6 ID:SGTC_3200|Compound:9114385|FD-Score:4.29|P-value:6.31E-5 ID:SGTC_3208|Compound:9114115|FD-Score:-3.44|P-value:7.57E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-3.78|P-value:2.55E-4 ID:SGTC_3277|Compound:9138724|FD-Score:-3.89|P-value:1.76E-4 ID:SGTC_3296|Compound:9122258|FD-Score:5.43|P-value:5.51E-7 ID:SGTC_53|Compound:0973-0023|FD-Score:-3.50|P-value:6.38E-4 ID:SGTC_100|Compound:0086-0128|FD-Score:3.81|P-value:3.37E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:-3.36|P-value:9.73E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:3.62|P-value:6.20E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:4.28|P-value:6.56E-5 ID:SGTC_173|Compound:1273-0061|FD-Score:3.67|P-value:5.25E-4 ID:SGTC_273|Compound:hydroxyurea|FD-Score:5.58|P-value:2.78E-7 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.48|P-value:6.72E-4 ID:SGTC_360|Compound:0957-0399|FD-Score:3.83|P-value:3.12E-4 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:3.62|P-value:6.23E-4 ID:SGTC_500|Compound:h-89|FD-Score:4.90|P-value:5.72E-6 ID:SGTC_547|Compound:1120-0019|FD-Score:-4.03|P-value:1.07E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-3.66|P-value:3.73E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:3.57|P-value:7.37E-4 ID:SGTC_582|Compound:0987-0078|FD-Score:3.99|P-value:1.85E-4 ID:SGTC_857|Compound:0438-0306|FD-Score:3.61|P-value:6.34E-4 ID:SGTC_1000|Compound:4048-0884|FD-Score:4.23|P-value:7.81E-5 ID:SGTC_1093|Compound:fluphenazine|FD-Score:5.13|P-value:2.19E-6 ID:SGTC_1473|Compound:k844-0725|FD-Score:3.83|P-value:3.17E-4 ID:SGTC_1508|Compound:1080-0625|FD-Score:3.77|P-value:3.81E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:3.73|P-value:4.42E-4 ID:SGTC_1629|Compound:st005085|FD-Score:4.77|P-value:9.80E-6 ID:SGTC_1688|Compound:st021091|FD-Score:3.78|P-value:3.66E-4 ID:SGTC_1723|Compound:st034307|FD-Score:4.26|P-value:6.98E-5 ID:SGTC_1834|Compound:st055981|FD-Score:4.70|P-value:1.30E-5 ID:SGTC_1884|Compound:5633444|FD-Score:5.68|P-value:1.78E-7 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:3.96|P-value:2.05E-4 ID:SGTC_2309|Compound:7664088|FD-Score:-3.57|P-value:4.99E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:3.89|P-value:2.59E-4 ID:SGTC_2750|Compound:bromebric acid|FD-Score:5.72|P-value:1.40E-7 ID:SGTC_2754|Compound:granisetron|FD-Score:-4.08|P-value:8.93E-5 ID:SGTC_2836|Compound:9010338|FD-Score:3.78|P-value:3.77E-4 ID:SGTC_2881|Compound:9058953|FD-Score:3.62|P-value:6.14E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-4.83|P-value:4.72E-6 ID:SGTC_3200|Compound:9114385|FD-Score:4.29|P-value:6.31E-5 ID:SGTC_3208|Compound:9114115|FD-Score:-3.44|P-value:7.57E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-3.78|P-value:2.55E-4 ID:SGTC_3277|Compound:9138724|FD-Score:-3.89|P-value:1.76E-4 ID:SGTC_3296|Compound:9122258|FD-Score:5.43|P-value:5.51E-7

Top fitness defect scores for YGR180C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2750 bromebric acid 5.72 70.2 µM 1.40E-7 6 5.17E-9
2 SGTC_1884 5633444 5.68 25.0 µM 1.78E-7 redox potentiating 30 6.93E-9
3 SGTC_273 hydroxyurea 5.58 18.1 mM 2.78E-7 heme biosynthesis & mitochondrial translocase 11 1.20E-8
4 SGTC_3296 9122258 5.43 64.0 µM 5.51E-7 redox potentiating 11 2.76E-8
5 SGTC_1093 fluphenazine 5.13 8.0 µM 2.19E-6 5 1.49E-7
6 SGTC_500 h-89 4.90 112.0 µM 5.72E-6 cell wall signaling 20 4.81E-7
7 SGTC_1629 st005085 4.77 44.9 µM 9.80E-6 RPP1 & pyrimidine depletion 15 9.29E-7
8 SGTC_1834 st055981 4.70 52.8 µM 1.30E-5 RPP1 & pyrimidine depletion 16 1.31E-6
9 SGTC_3200 9114385 4.29 49.5 µM 6.31E-5 23 9.03E-6
10 SGTC_157 1348-1695 4.28 65.8 µM 6.56E-5 iron homeostasis 53 9.48E-6
11 SGTC_1723 st034307 4.26 2.5 µM 6.98E-5 mitochondrial processes 21 1.02E-5
12 SGTC_1000 4048-0884 4.23 8.7 µM 7.81E-5 copper-dependent oxidative stress 27 1.17E-5
13 SGTC_582 0987-0078 3.99 156.0 µM 1.85E-4 calcium & mitochondrial duress 69 3.35E-5
14 SGTC_2169 5-(ethoxymethyl)quinolin-8-ol 3.96 49.5 µM 2.05E-4 22 3.80E-5
15 SGTC_2737 bleomycin 3.89 30.5 nM 2.59E-4 Golgi 89 5.07E-5
16 SGTC_360 0957-0399 3.83 1.0 µM 3.12E-4 cell wall 49 6.35E-5
17 SGTC_1473 k844-0725 3.83 134.0 µM 3.17E-4 15 6.47E-5
18 SGTC_100 0086-0128 3.81 49.6 µM 3.37E-4 copper-dependent oxidative stress 55 6.98E-5
19 SGTC_1688 st021091 3.78 57.6 µM 3.66E-4 7 7.72E-5
20 SGTC_2836 9010338 3.78 19.5 µM 3.77E-4 RPP1 & pyrimidine depletion 32 8.00E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.147 9.99E-18 YJL027C_p YJL027C_p hom Putative protein of unknown function
0.124 5.21E-13 YGL168W HUR1 hom Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene
0.106 8.17E-10 YDR478W SNM1 het Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP
0.105 1.10E-9 YGL071W AFT1 hom Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.105 1.11E-9 YOR256C TRE2 het Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication
0.101 4.69E-9 YPL161C BEM4 hom Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length
0.100 6.30E-9 YGL127C SOH1 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits
0.096 2.38E-8 YGL167C PMR1 hom High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease
0.095 4.00E-8 YDR028C REG1 hom Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p
0.095 4.30E-8 YMR077C VPS20 hom Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes
0.093 7.01E-8 YNL236W SIN4 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription
0.092 8.13E-8 YER077C_p YER077C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport
0.091 1.22E-7 YHR008C SOD2 hom Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated
0.089 2.79E-7 YMR125W STO1 hom Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80
0.088 3.16E-7 YDL102W POL3 het Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)