YGR184C / UBR1

E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS)

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR184C / UBR1

Click on Significant Values for Screen Details ID:SGTC_7|Compound:0192-1582|FD-Score:3.73|P-value:2.02E-4 ID:SGTC_20|Compound:0481-0013|FD-Score:3.44|P-value:5.46E-4 ID:SGTC_32|Compound:0928-0142|FD-Score:-3.82|P-value:1.32E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.26|P-value:9.03E-4 ID:SGTC_92|Compound:0063-0024|FD-Score:3.34|P-value:7.57E-4 ID:SGTC_220|Compound:paclitaxel|FD-Score:-3.52|P-value:3.85E-4 ID:SGTC_223|Compound:busulfan|FD-Score:-3.24|P-value:9.62E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:3.30|P-value:8.67E-4 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:3.73|P-value:2.00E-4 ID:SGTC_250|Compound:brefeldin a|FD-Score:3.64|P-value:2.75E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.97|P-value:8.19E-5 ID:SGTC_263|Compound:alizarin|FD-Score:3.38|P-value:6.79E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:3.44|P-value:5.44E-4 ID:SGTC_492|Compound:tmb-8|FD-Score:3.43|P-value:5.61E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:5.41|P-value:1.36E-7 ID:SGTC_662|Compound:k832-1615|FD-Score:3.44|P-value:5.50E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:-3.57|P-value:3.18E-4 ID:SGTC_819|Compound:1013-0258|FD-Score:4.15|P-value:4.06E-5 ID:SGTC_851|Compound:0394-0702|FD-Score:-3.99|P-value:6.85E-5 ID:SGTC_858|Compound:0438-0351|FD-Score:4.56|P-value:7.53E-6 ID:SGTC_957|Compound:1187-1561|FD-Score:-5.09|P-value:5.74E-7 ID:SGTC_1114|Compound:scopoletin|FD-Score:-5.11|P-value:5.15E-7 ID:SGTC_1129|Compound:3453-1384|FD-Score:3.38|P-value:6.59E-4 ID:SGTC_1160|Compound:4092-0570|FD-Score:3.88|P-value:1.15E-4 ID:SGTC_1241|Compound:0364-0022|FD-Score:3.85|P-value:1.27E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:3.54|P-value:3.92E-4 ID:SGTC_1745|Compound:st081495|FD-Score:5.37|P-value:1.66E-7 ID:SGTC_1778|Compound:st046819|FD-Score:4.24|P-value:2.87E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:-3.68|P-value:2.20E-4 ID:SGTC_1920|Compound:st075728|FD-Score:-3.53|P-value:3.73E-4 ID:SGTC_2010|Compound:4012422|FD-Score:4.17|P-value:3.68E-5 ID:SGTC_2160|Compound:5660466|FD-Score:-3.76|P-value:1.63E-4 ID:SGTC_2162|Compound:5663267|FD-Score:-3.65|P-value:2.44E-4 ID:SGTC_2166|Compound:5933061|FD-Score:-4.80|P-value:2.29E-6 ID:SGTC_2192|Compound:5915882|FD-Score:3.33|P-value:7.84E-4 ID:SGTC_2434|Compound:5670830|FD-Score:3.31|P-value:8.39E-4 ID:SGTC_2523|Compound:spermine|FD-Score:3.47|P-value:4.87E-4 ID:SGTC_2585|Compound:alliin|FD-Score:3.62|P-value:2.90E-4 ID:SGTC_2730|Compound:imipramine|FD-Score:5.72|P-value:2.72E-8 ID:SGTC_2741|Compound:miltefosine|FD-Score:3.35|P-value:7.49E-4 ID:SGTC_2742|Compound:clothiapine|FD-Score:4.39|P-value:1.49E-5 ID:SGTC_2744|Compound:captopril|FD-Score:3.34|P-value:7.55E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:3.40|P-value:6.18E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.75|P-value:1.85E-4 ID:SGTC_2958|Compound:9077254|FD-Score:-3.49|P-value:4.22E-4 ID:SGTC_2961|Compound:9083290|FD-Score:-3.32|P-value:7.36E-4 ID:SGTC_3075|Compound:9116269|FD-Score:4.80|P-value:2.58E-6 ID:SGTC_3196|Compound:9111891|FD-Score:-3.49|P-value:4.15E-4 ID:SGTC_3198|Compound:9112019|FD-Score:-3.46|P-value:4.71E-4 ID:SGTC_3259|Compound:9138485|FD-Score:-3.55|P-value:3.47E-4 ID:SGTC_3303|Compound:9125618|FD-Score:4.08|P-value:5.31E-5 ID:SGTC_7|Compound:0192-1582|FD-Score:3.73|P-value:2.02E-4 ID:SGTC_20|Compound:0481-0013|FD-Score:3.44|P-value:5.46E-4 ID:SGTC_32|Compound:0928-0142|FD-Score:-3.82|P-value:1.32E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.26|P-value:9.03E-4 ID:SGTC_92|Compound:0063-0024|FD-Score:3.34|P-value:7.57E-4 ID:SGTC_220|Compound:paclitaxel|FD-Score:-3.52|P-value:3.85E-4 ID:SGTC_223|Compound:busulfan|FD-Score:-3.24|P-value:9.62E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:3.30|P-value:8.67E-4 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:3.73|P-value:2.00E-4 ID:SGTC_250|Compound:brefeldin a|FD-Score:3.64|P-value:2.75E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.97|P-value:8.19E-5 ID:SGTC_263|Compound:alizarin|FD-Score:3.38|P-value:6.79E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:3.44|P-value:5.44E-4 ID:SGTC_492|Compound:tmb-8|FD-Score:3.43|P-value:5.61E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:5.41|P-value:1.36E-7 ID:SGTC_662|Compound:k832-1615|FD-Score:3.44|P-value:5.50E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:-3.57|P-value:3.18E-4 ID:SGTC_819|Compound:1013-0258|FD-Score:4.15|P-value:4.06E-5 ID:SGTC_851|Compound:0394-0702|FD-Score:-3.99|P-value:6.85E-5 ID:SGTC_858|Compound:0438-0351|FD-Score:4.56|P-value:7.53E-6 ID:SGTC_957|Compound:1187-1561|FD-Score:-5.09|P-value:5.74E-7 ID:SGTC_1114|Compound:scopoletin|FD-Score:-5.11|P-value:5.15E-7 ID:SGTC_1129|Compound:3453-1384|FD-Score:3.38|P-value:6.59E-4 ID:SGTC_1160|Compound:4092-0570|FD-Score:3.88|P-value:1.15E-4 ID:SGTC_1241|Compound:0364-0022|FD-Score:3.85|P-value:1.27E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:3.54|P-value:3.92E-4 ID:SGTC_1745|Compound:st081495|FD-Score:5.37|P-value:1.66E-7 ID:SGTC_1778|Compound:st046819|FD-Score:4.24|P-value:2.87E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:-3.68|P-value:2.20E-4 ID:SGTC_1920|Compound:st075728|FD-Score:-3.53|P-value:3.73E-4 ID:SGTC_2010|Compound:4012422|FD-Score:4.17|P-value:3.68E-5 ID:SGTC_2160|Compound:5660466|FD-Score:-3.76|P-value:1.63E-4 ID:SGTC_2162|Compound:5663267|FD-Score:-3.65|P-value:2.44E-4 ID:SGTC_2166|Compound:5933061|FD-Score:-4.80|P-value:2.29E-6 ID:SGTC_2192|Compound:5915882|FD-Score:3.33|P-value:7.84E-4 ID:SGTC_2434|Compound:5670830|FD-Score:3.31|P-value:8.39E-4 ID:SGTC_2523|Compound:spermine|FD-Score:3.47|P-value:4.87E-4 ID:SGTC_2585|Compound:alliin|FD-Score:3.62|P-value:2.90E-4 ID:SGTC_2730|Compound:imipramine|FD-Score:5.72|P-value:2.72E-8 ID:SGTC_2741|Compound:miltefosine|FD-Score:3.35|P-value:7.49E-4 ID:SGTC_2742|Compound:clothiapine|FD-Score:4.39|P-value:1.49E-5 ID:SGTC_2744|Compound:captopril|FD-Score:3.34|P-value:7.55E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:3.40|P-value:6.18E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.75|P-value:1.85E-4 ID:SGTC_2958|Compound:9077254|FD-Score:-3.49|P-value:4.22E-4 ID:SGTC_2961|Compound:9083290|FD-Score:-3.32|P-value:7.36E-4 ID:SGTC_3075|Compound:9116269|FD-Score:4.80|P-value:2.58E-6 ID:SGTC_3196|Compound:9111891|FD-Score:-3.49|P-value:4.15E-4 ID:SGTC_3198|Compound:9112019|FD-Score:-3.46|P-value:4.71E-4 ID:SGTC_3259|Compound:9138485|FD-Score:-3.55|P-value:3.47E-4 ID:SGTC_3303|Compound:9125618|FD-Score:4.08|P-value:5.31E-5

Top fitness defect scores for YGR184C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2730 imipramine 5.72 71.3 µM 2.72E-8 3 5.36E-9
2 SGTC_660 4031-1742 5.41 102.0 µM 1.36E-7 redox potentiating 14 3.16E-8
3 SGTC_1745 st081495 5.37 74.0 µM 1.66E-7 RNA processing & uracil transport 35 3.93E-8
4 SGTC_3075 9116269 4.80 49.5 µM 2.58E-6 12 8.04E-7
5 SGTC_858 0438-0351 4.56 2.8 µM 7.53E-6 copper-dependent oxidative stress 10 2.61E-6
6 SGTC_2742 clothiapine 4.39 36.3 µM 1.49E-5 NEO1 5 5.54E-6
7 SGTC_1778 st046819 4.24 58.9 µM 2.87E-5 18 1.14E-5
8 SGTC_2010 4012422 4.17 159.0 µM 3.68E-5 19 1.50E-5
9 SGTC_819 1013-0258 4.15 27.7 µM 4.06E-5 34 1.66E-5
10 SGTC_3303 9125618 4.08 40.6 µM 5.31E-5 39 2.24E-5
11 SGTC_255 tunicamycin 3.97 200.5 nM 8.19E-5 unfolded protein response 72 3.61E-5
12 SGTC_1160 4092-0570 3.88 10.5 µM 1.15E-4 plasma membrane duress 41 5.22E-5
13 SGTC_1241 0364-0022 3.85 50.8 µM 1.27E-4 ERG2 32 5.81E-5
14 SGTC_2778 tioconazole 3.75 65.1 nM 1.85E-4 ergosterol depletion effects on membrane 70 8.83E-5
15 SGTC_239 5hr heat shock (37°C) + mitomycin C 3.73 819.4 µM 2.00E-4 heat shock/prefoldin 40 9.60E-5
16 SGTC_7 0192-1582 3.73 4.2 µM 2.02E-4 70 9.71E-5
17 SGTC_250 brefeldin a 3.64 552.2 µM 2.75E-4 PDR1 20 1.36E-4
18 SGTC_2585 alliin 3.62 100.0 µM 2.90E-4 38 1.45E-4
19 SGTC_1261 0712-0010 3.54 12.7 µM 3.92E-4 RNA processing & uracil transport 37 2.01E-4
20 SGTC_2523 spermine 3.47 47.7 µM 4.87E-4 calcium & mitochondrial duress 37 2.56E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.118 7.35E-12 YDR290W_d YDR290W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103
0.118 8.06E-12 YDR032C PST2 hom Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication
0.106 8.27E-10 YNL009W IDP3 hom Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication
0.105 1.18E-9 YNL244C SUI1 het Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase
0.104 1.50E-9 YEL031W SPF1 hom P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1
0.099 8.80E-9 YLR014C PPR1 hom Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p
0.096 2.46E-8 YDR289C RTT103 hom Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition
0.095 3.47E-8 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.095 4.28E-8 YGL237C HAP2 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding
0.092 1.11E-7 YBR243C ALG7 het UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
0.091 1.21E-7 YML081C-A ATP18 hom Subunit of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; termed subunit I or subunit j; does not correspond to known ATP synthase subunits in other organisms
0.090 1.78E-7 YDR010C_d YDR010C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.086 5.98E-7 YKR072C SIS2 hom Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis
0.085 8.54E-7 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.084 1.13E-6 YPL131W RPL5 het Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly