HIPHOP chemogenomics database

YGR199W / PMT6

Protein O-mannosyltransferase, transfers mannose from dolichyl phosphate-D-mannose to protein serine/threonine residues of secretory proteins; reaction is essential for cell wall rigidity; member of a family of mannosyltransferases

Zygosity: Homozygous strain

fixedexpanded
Profile for YGR199W / PMT6

Click on Significant Values for Screen Details

Top fitness defect scores for YGR199W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1310	1188-0297	5.00	994.0 nM	4.12E-8	azole & statin	16	2.85E-7
2	SGTC_326	k292-0812	4.75	17.5 µM	1.78E-7		2	1.02E-6
3	SGTC_928	2764-0428	4.25	58.7 µM	2.62E-6	cell wall	49	1.05E-5
4	SGTC_1594	piperine	3.86	70.1 µM	1.78E-5		15	5.57E-5
5	SGTC_2837	9008476	3.68	45.5 µM	4.25E-5		44	1.19E-4
6	SGTC_2987	9018338	3.67	71.4 µM	4.34E-5	RPP1 & pyrimidine depletion	29	1.21E-4
7	SGTC_626	0214-0009	3.64	33.0 µM	4.89E-5	copper-dependent oxidative stress	27	1.34E-4
8	SGTC_453	enantio-paf c16	3.61	10.0 µM	5.71E-5		175	1.54E-4
9	SGTC_841	0251-0215	3.53	91.2 µM	7.91E-5		85	2.04E-4
10	SGTC_2621	lithochol-11-enic acid	3.30	100.0 µM	2.10E-4		56	4.79E-4
11	SGTC_1633	st005664	3.28	67.9 µM	2.31E-4		69	5.20E-4
12	SGTC_281	1672-3440	3.07	14.9 µM	5.35E-4	Golgi	28	0.00109
13	SGTC_1578	josamycin	3.06	24.2 µM	5.48E-4		40	0.00111
14	SGTC_2208	7283366	3.02	200.0 µM	6.44E-4		37	0.00128
15	SGTC_457	grayanotoxin iii	2.98	135.0 µM	7.28E-4		143	0.00142
16	SGTC_2620	irigenin	2.94	100.0 µM	8.47E-4		88	0.00162
17	SGTC_640	3381-0960	2.88	37.1 µM	0.00107		52	0.00199
18	SGTC_847	0342-0274	2.85	5.5 µM	0.00120		71	0.00220
19	SGTC_2280	7785178	2.82	200.0 µM	0.00132		48	0.00240
20	SGTC_678	1218-2179	2.80	85.9 µM	0.00142	RNA pol III & RNase P/MRP	29	0.00255

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.222	8.52E-39	YJR137C	MET5	hom	Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine
0.150	2.20E-18	YML101C-A_d	YML101C-A_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.127	1.31E-13	YOR301W	RAX1	hom	Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily
0.122	1.25E-12	YGR100W	MDR1	hom	Cytoplasmic GTPase-activating protein for Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function
0.115	2.58E-11	YJR078W	BNA2	hom	Putative tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase, required for de novo biosynthesis of NAD from tryptophan via kynurenine; interacts genetically with telomere capping gene CDC13; regulated by Hst1p and Aftp
0.115	2.77E-11	YGR136W	LSB1	hom	Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress
0.103	2.27E-9	YDR223W	CRF1	hom	Transcriptional corepressor involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain
0.102	2.87E-9	YDL188C	PPH22	hom	Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; PPH22 has a paralog, PPH21, that arose from the whole genome duplication
0.099	8.95E-9	YGR010W	NMA2	hom	Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in de novo and salvage synthesis of NAD(+); NMA2 has a paralog, NMA1, that arose from the whole genome duplication
0.098	1.40E-8	YOR176W	HEM15	het	Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway
0.095	3.44E-8	YBR071W	YBR071W	hom	Protein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking
0.095	3.87E-8	YIL173W	VTH1	hom	Putative membrane glycoprotein with strong similarity to Vth2p and Pep1p/Vps10p, may be involved in vacuolar protein sorting
0.094	4.48E-8	YOL125W	TRM13	hom	2'-O-methyltransferase responsible for modification of tRNA at position 4; C-terminal domain has similarity to Rossmann-fold (RFM) superfamily of RNA methyltransferases
0.092	9.60E-8	YHR063C	PAN5	hom	2-dehydropantoate 2-reductase, part of the pantothenic acid pathway, structurally homologous to E. coli panE
0.089	2.16E-7	YBL005W	PDR3	hom	Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome; PDR3 has a paralog, PDR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress