YGR199W / PMT6

Protein O-mannosyltransferase, transfers mannose from dolichyl phosphate-D-mannose to protein serine/threonine residues of secretory proteins; reaction is essential for cell wall rigidity; member of a family of mannosyltransferases

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR199W / PMT6

Click on Significant Values for Screen Details ID:SGTC_264|Compound:promethazine|FD-Score:-3.71|P-value:2.55E-5 ID:SGTC_281|Compound:1672-3440|FD-Score:3.07|P-value:5.35E-4 ID:SGTC_297|Compound:0986-0032|FD-Score:-2.91|P-value:7.13E-4 ID:SGTC_326|Compound:k292-0812|FD-Score:4.75|P-value:1.78E-7 ID:SGTC_331|Compound:k048-0133|FD-Score:-3.36|P-value:1.24E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:-2.87|P-value:8.52E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.61|P-value:5.71E-5 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:2.98|P-value:7.28E-4 ID:SGTC_626|Compound:0214-0009|FD-Score:3.64|P-value:4.89E-5 ID:SGTC_841|Compound:0251-0215|FD-Score:3.53|P-value:7.91E-5 ID:SGTC_864|Compound:0469-0573|FD-Score:-3.22|P-value:2.17E-4 ID:SGTC_905|Compound:2-aminofluorene|FD-Score:-2.90|P-value:7.65E-4 ID:SGTC_928|Compound:2764-0428|FD-Score:4.25|P-value:2.62E-6 ID:SGTC_1310|Compound:1188-0297|FD-Score:5.00|P-value:4.12E-8 ID:SGTC_1397|Compound:3532-0190|FD-Score:-3.20|P-value:2.34E-4 ID:SGTC_1578|Compound:josamycin|FD-Score:3.06|P-value:5.48E-4 ID:SGTC_1594|Compound:piperine|FD-Score:3.86|P-value:1.78E-5 ID:SGTC_1633|Compound:st005664|FD-Score:3.28|P-value:2.31E-4 ID:SGTC_1828|Compound:st055811|FD-Score:-2.97|P-value:5.78E-4 ID:SGTC_1832|Compound:st054113|FD-Score:-4.86|P-value:5.94E-8 ID:SGTC_1841|Compound:st054148|FD-Score:-2.88|P-value:8.21E-4 ID:SGTC_2052|Compound:5265182|FD-Score:-3.48|P-value:7.26E-5 ID:SGTC_2128|Compound:5255449|FD-Score:-3.33|P-value:1.40E-4 ID:SGTC_2129|Compound:girgensonine|FD-Score:-3.39|P-value:1.07E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:-3.10|P-value:3.55E-4 ID:SGTC_2208|Compound:7283366|FD-Score:3.02|P-value:6.44E-4 ID:SGTC_2289|Compound:7946404|FD-Score:-4.64|P-value:2.24E-7 ID:SGTC_2543|Compound:pimpinellin|FD-Score:-2.84|P-value:9.49E-4 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:-3.07|P-value:3.89E-4 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:-3.83|P-value:1.51E-5 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:-3.07|P-value:3.99E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:2.94|P-value:8.47E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.30|P-value:2.10E-4 ID:SGTC_2837|Compound:9008476|FD-Score:3.68|P-value:4.25E-5 ID:SGTC_2987|Compound:9018338|FD-Score:3.67|P-value:4.34E-5 ID:SGTC_3180|Compound:9109287|FD-Score:-3.42|P-value:9.25E-5 ID:SGTC_264|Compound:promethazine|FD-Score:-3.71|P-value:2.55E-5 ID:SGTC_281|Compound:1672-3440|FD-Score:3.07|P-value:5.35E-4 ID:SGTC_297|Compound:0986-0032|FD-Score:-2.91|P-value:7.13E-4 ID:SGTC_326|Compound:k292-0812|FD-Score:4.75|P-value:1.78E-7 ID:SGTC_331|Compound:k048-0133|FD-Score:-3.36|P-value:1.24E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:-2.87|P-value:8.52E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.61|P-value:5.71E-5 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:2.98|P-value:7.28E-4 ID:SGTC_626|Compound:0214-0009|FD-Score:3.64|P-value:4.89E-5 ID:SGTC_841|Compound:0251-0215|FD-Score:3.53|P-value:7.91E-5 ID:SGTC_864|Compound:0469-0573|FD-Score:-3.22|P-value:2.17E-4 ID:SGTC_905|Compound:2-aminofluorene|FD-Score:-2.90|P-value:7.65E-4 ID:SGTC_928|Compound:2764-0428|FD-Score:4.25|P-value:2.62E-6 ID:SGTC_1310|Compound:1188-0297|FD-Score:5.00|P-value:4.12E-8 ID:SGTC_1397|Compound:3532-0190|FD-Score:-3.20|P-value:2.34E-4 ID:SGTC_1578|Compound:josamycin|FD-Score:3.06|P-value:5.48E-4 ID:SGTC_1594|Compound:piperine|FD-Score:3.86|P-value:1.78E-5 ID:SGTC_1633|Compound:st005664|FD-Score:3.28|P-value:2.31E-4 ID:SGTC_1828|Compound:st055811|FD-Score:-2.97|P-value:5.78E-4 ID:SGTC_1832|Compound:st054113|FD-Score:-4.86|P-value:5.94E-8 ID:SGTC_1841|Compound:st054148|FD-Score:-2.88|P-value:8.21E-4 ID:SGTC_2052|Compound:5265182|FD-Score:-3.48|P-value:7.26E-5 ID:SGTC_2128|Compound:5255449|FD-Score:-3.33|P-value:1.40E-4 ID:SGTC_2129|Compound:girgensonine|FD-Score:-3.39|P-value:1.07E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:-3.10|P-value:3.55E-4 ID:SGTC_2208|Compound:7283366|FD-Score:3.02|P-value:6.44E-4 ID:SGTC_2289|Compound:7946404|FD-Score:-4.64|P-value:2.24E-7 ID:SGTC_2543|Compound:pimpinellin|FD-Score:-2.84|P-value:9.49E-4 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:-3.07|P-value:3.89E-4 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:-3.83|P-value:1.51E-5 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:-3.07|P-value:3.99E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:2.94|P-value:8.47E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.30|P-value:2.10E-4 ID:SGTC_2837|Compound:9008476|FD-Score:3.68|P-value:4.25E-5 ID:SGTC_2987|Compound:9018338|FD-Score:3.67|P-value:4.34E-5 ID:SGTC_3180|Compound:9109287|FD-Score:-3.42|P-value:9.25E-5

Top fitness defect scores for YGR199W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1310 1188-0297 5.00 994.0 nM 4.12E-8 azole & statin 16 2.85E-7
2 SGTC_326 k292-0812 4.75 17.5 µM 1.78E-7 2 1.02E-6
3 SGTC_928 2764-0428 4.25 58.7 µM 2.62E-6 cell wall 49 1.05E-5
4 SGTC_1594 piperine 3.86 70.1 µM 1.78E-5 15 5.57E-5
5 SGTC_2837 9008476 3.68 45.5 µM 4.25E-5 44 1.19E-4
6 SGTC_2987 9018338 3.67 71.4 µM 4.34E-5 RPP1 & pyrimidine depletion 29 1.21E-4
7 SGTC_626 0214-0009 3.64 33.0 µM 4.89E-5 copper-dependent oxidative stress 27 1.34E-4
8 SGTC_453 enantio-paf c16 3.61 10.0 µM 5.71E-5 175 1.54E-4
9 SGTC_841 0251-0215 3.53 91.2 µM 7.91E-5 85 2.04E-4
10 SGTC_2621 lithochol-11-enic acid 3.30 100.0 µM 2.10E-4 56 4.79E-4
11 SGTC_1633 st005664 3.28 67.9 µM 2.31E-4 69 5.20E-4
12 SGTC_281 1672-3440 3.07 14.9 µM 5.35E-4 Golgi 28 0.00109
13 SGTC_1578 josamycin 3.06 24.2 µM 5.48E-4 40 0.00111
14 SGTC_2208 7283366 3.02 200.0 µM 6.44E-4 37 0.00128
15 SGTC_457 grayanotoxin iii 2.98 135.0 µM 7.28E-4 143 0.00142
16 SGTC_2620 irigenin 2.94 100.0 µM 8.47E-4 88 0.00162
17 SGTC_640 3381-0960 2.88 37.1 µM 0.00107 52 0.00199
18 SGTC_847 0342-0274 2.85 5.5 µM 0.00120 71 0.00220
19 SGTC_2280 7785178 2.82 200.0 µM 0.00132 48 0.00240
20 SGTC_678 1218-2179 2.80 85.9 µM 0.00142 RNA pol III & RNase P/MRP 29 0.00255

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.222 8.52E-39 YJR137C MET5 hom Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine
0.150 2.20E-18 YML101C-A_d YML101C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.127 1.31E-13 YOR301W RAX1 hom Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily
0.122 1.25E-12 YGR100W MDR1 hom Cytoplasmic GTPase-activating protein for Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function
0.115 2.58E-11 YJR078W BNA2 hom Putative tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase, required for de novo biosynthesis of NAD from tryptophan via kynurenine; interacts genetically with telomere capping gene CDC13; regulated by Hst1p and Aftp
0.115 2.77E-11 YGR136W LSB1 hom Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress
0.103 2.27E-9 YDR223W CRF1 hom Transcriptional corepressor involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain
0.102 2.87E-9 YDL188C PPH22 hom Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; PPH22 has a paralog, PPH21, that arose from the whole genome duplication
0.099 8.95E-9 YGR010W NMA2 hom Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in de novo and salvage synthesis of NAD(+); NMA2 has a paralog, NMA1, that arose from the whole genome duplication
0.098 1.40E-8 YOR176W HEM15 het Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway
0.095 3.44E-8 YBR071W YBR071W hom Protein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking
0.095 3.87E-8 YIL173W VTH1 hom Putative membrane glycoprotein with strong similarity to Vth2p and Pep1p/Vps10p, may be involved in vacuolar protein sorting
0.094 4.48E-8 YOL125W TRM13 hom 2'-O-methyltransferase responsible for modification of tRNA at position 4; C-terminal domain has similarity to Rossmann-fold (RFM) superfamily of RNA methyltransferases
0.092 9.60E-8 YHR063C PAN5 hom 2-dehydropantoate 2-reductase, part of the pantothenic acid pathway, structurally homologous to E. coli panE
0.089 2.16E-7 YBL005W PDR3 hom Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome; PDR3 has a paralog, PDR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress