YGR258C / RAD2

Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR258C / RAD2

Click on Significant Values for Screen Details ID:SGTC_227|Compound:fluconazole|FD-Score:-3.01|P-value:3.89E-4 ID:SGTC_247|Compound:mechlorethamine|FD-Score:5.82|P-value:2.32E-11 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:3.08|P-value:2.32E-4 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:26.70|P-value:6.50E-200 ID:SGTC_550|Compound:4123-0385|FD-Score:2.84|P-value:6.18E-4 ID:SGTC_645|Compound:0845-0943|FD-Score:-2.92|P-value:5.42E-4 ID:SGTC_954|Compound:1189-0853|FD-Score:-3.84|P-value:8.42E-6 ID:SGTC_957|Compound:1187-1561|FD-Score:-3.12|P-value:2.46E-4 ID:SGTC_990|Compound:3985-0011|FD-Score:-4.19|P-value:1.34E-6 ID:SGTC_997|Compound:3996-0078|FD-Score:-3.41|P-value:6.77E-5 ID:SGTC_1002|Compound:4049-0206|FD-Score:-4.26|P-value:8.81E-7 ID:SGTC_1056|Compound:3448-1962|FD-Score:-3.00|P-value:4.03E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:-3.64|P-value:2.31E-5 ID:SGTC_1104|Compound:tacrolimus|FD-Score:-3.05|P-value:3.24E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-3.17|P-value:1.93E-4 ID:SGTC_1390|Compound:3413-2614|FD-Score:2.89|P-value:5.18E-4 ID:SGTC_1401|Compound:3807-4679|FD-Score:-3.08|P-value:2.83E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-5.58|P-value:1.90E-10 ID:SGTC_1463|Compound:k081-0032|FD-Score:-5.98|P-value:9.30E-12 ID:SGTC_1709|Compound:st033179|FD-Score:-3.14|P-value:2.22E-4 ID:SGTC_1754|Compound:st042065|FD-Score:3.99|P-value:3.01E-6 ID:SGTC_1800|Compound:st050079|FD-Score:2.77|P-value:8.29E-4 ID:SGTC_1801|Compound:st050089|FD-Score:3.73|P-value:1.15E-5 ID:SGTC_1804|Compound:st050076|FD-Score:3.08|P-value:2.38E-4 ID:SGTC_1965|Compound:Pectolinarigenin|FD-Score:-2.93|P-value:5.30E-4 ID:SGTC_1986|Compound:st071812|FD-Score:-2.84|P-value:7.49E-4 ID:SGTC_2245|Compound:6637752|FD-Score:2.94|P-value:4.27E-4 ID:SGTC_2304|Compound:7443016|FD-Score:3.17|P-value:1.61E-4 ID:SGTC_2331|Compound:7980721|FD-Score:-2.99|P-value:4.16E-4 ID:SGTC_2369|Compound:9071472|FD-Score:3.39|P-value:6.12E-5 ID:SGTC_2393|Compound:9031399|FD-Score:3.06|P-value:2.58E-4 ID:SGTC_2402|Compound:5553605|FD-Score:3.28|P-value:9.68E-5 ID:SGTC_2408|Compound:sulbentine|FD-Score:2.86|P-value:5.86E-4 ID:SGTC_2421|Compound:5690543|FD-Score:3.36|P-value:6.84E-5 ID:SGTC_2424|Compound:5537928|FD-Score:2.96|P-value:3.90E-4 ID:SGTC_2435|Compound:5706476|FD-Score:2.77|P-value:8.31E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:-3.46|P-value:5.33E-5 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.13|P-value:1.89E-4 ID:SGTC_2677|Compound:mitomycin C|FD-Score:3.50|P-value:3.57E-5 ID:SGTC_2687|Compound:chenodiol|FD-Score:3.02|P-value:3.04E-4 ID:SGTC_2690|Compound:usnic acid|FD-Score:4.65|P-value:6.91E-8 ID:SGTC_2691|Compound:st077356|FD-Score:-2.97|P-value:4.46E-4 ID:SGTC_2692|Compound:st077426|FD-Score:2.75|P-value:9.05E-4 ID:SGTC_2693|Compound:st077581|FD-Score:4.14|P-value:1.32E-6 ID:SGTC_2839|Compound:9003951|FD-Score:2.83|P-value:6.61E-4 ID:SGTC_2971|Compound:9092186|FD-Score:3.16|P-value:1.64E-4 ID:SGTC_3193|Compound:9111931|FD-Score:-2.87|P-value:6.77E-4 ID:SGTC_3292|Compound:9120825|FD-Score:3.29|P-value:9.54E-5 ID:SGTC_3311|Compound:9131146|FD-Score:-2.94|P-value:5.10E-4 ID:SGTC_3321|Compound:9137793|FD-Score:-3.50|P-value:4.47E-5 ID:SGTC_227|Compound:fluconazole|FD-Score:-3.01|P-value:3.89E-4 ID:SGTC_247|Compound:mechlorethamine|FD-Score:5.82|P-value:2.32E-11 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:3.08|P-value:2.32E-4 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:26.70|P-value:6.50E-200 ID:SGTC_550|Compound:4123-0385|FD-Score:2.84|P-value:6.18E-4 ID:SGTC_645|Compound:0845-0943|FD-Score:-2.92|P-value:5.42E-4 ID:SGTC_954|Compound:1189-0853|FD-Score:-3.84|P-value:8.42E-6 ID:SGTC_957|Compound:1187-1561|FD-Score:-3.12|P-value:2.46E-4 ID:SGTC_990|Compound:3985-0011|FD-Score:-4.19|P-value:1.34E-6 ID:SGTC_997|Compound:3996-0078|FD-Score:-3.41|P-value:6.77E-5 ID:SGTC_1002|Compound:4049-0206|FD-Score:-4.26|P-value:8.81E-7 ID:SGTC_1056|Compound:3448-1962|FD-Score:-3.00|P-value:4.03E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:-3.64|P-value:2.31E-5 ID:SGTC_1104|Compound:tacrolimus|FD-Score:-3.05|P-value:3.24E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-3.17|P-value:1.93E-4 ID:SGTC_1390|Compound:3413-2614|FD-Score:2.89|P-value:5.18E-4 ID:SGTC_1401|Compound:3807-4679|FD-Score:-3.08|P-value:2.83E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-5.58|P-value:1.90E-10 ID:SGTC_1463|Compound:k081-0032|FD-Score:-5.98|P-value:9.30E-12 ID:SGTC_1709|Compound:st033179|FD-Score:-3.14|P-value:2.22E-4 ID:SGTC_1754|Compound:st042065|FD-Score:3.99|P-value:3.01E-6 ID:SGTC_1800|Compound:st050079|FD-Score:2.77|P-value:8.29E-4 ID:SGTC_1801|Compound:st050089|FD-Score:3.73|P-value:1.15E-5 ID:SGTC_1804|Compound:st050076|FD-Score:3.08|P-value:2.38E-4 ID:SGTC_1965|Compound:Pectolinarigenin|FD-Score:-2.93|P-value:5.30E-4 ID:SGTC_1986|Compound:st071812|FD-Score:-2.84|P-value:7.49E-4 ID:SGTC_2245|Compound:6637752|FD-Score:2.94|P-value:4.27E-4 ID:SGTC_2304|Compound:7443016|FD-Score:3.17|P-value:1.61E-4 ID:SGTC_2331|Compound:7980721|FD-Score:-2.99|P-value:4.16E-4 ID:SGTC_2369|Compound:9071472|FD-Score:3.39|P-value:6.12E-5 ID:SGTC_2393|Compound:9031399|FD-Score:3.06|P-value:2.58E-4 ID:SGTC_2402|Compound:5553605|FD-Score:3.28|P-value:9.68E-5 ID:SGTC_2408|Compound:sulbentine|FD-Score:2.86|P-value:5.86E-4 ID:SGTC_2421|Compound:5690543|FD-Score:3.36|P-value:6.84E-5 ID:SGTC_2424|Compound:5537928|FD-Score:2.96|P-value:3.90E-4 ID:SGTC_2435|Compound:5706476|FD-Score:2.77|P-value:8.31E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:-3.46|P-value:5.33E-5 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.13|P-value:1.89E-4 ID:SGTC_2677|Compound:mitomycin C|FD-Score:3.50|P-value:3.57E-5 ID:SGTC_2687|Compound:chenodiol|FD-Score:3.02|P-value:3.04E-4 ID:SGTC_2690|Compound:usnic acid|FD-Score:4.65|P-value:6.91E-8 ID:SGTC_2691|Compound:st077356|FD-Score:-2.97|P-value:4.46E-4 ID:SGTC_2692|Compound:st077426|FD-Score:2.75|P-value:9.05E-4 ID:SGTC_2693|Compound:st077581|FD-Score:4.14|P-value:1.32E-6 ID:SGTC_2839|Compound:9003951|FD-Score:2.83|P-value:6.61E-4 ID:SGTC_2971|Compound:9092186|FD-Score:3.16|P-value:1.64E-4 ID:SGTC_3193|Compound:9111931|FD-Score:-2.87|P-value:6.77E-4 ID:SGTC_3292|Compound:9120825|FD-Score:3.29|P-value:9.54E-5 ID:SGTC_3311|Compound:9131146|FD-Score:-2.94|P-value:5.10E-4 ID:SGTC_3321|Compound:9137793|FD-Score:-3.50|P-value:4.47E-5

Top fitness defect scores for YGR258C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_267 4-Nitroquinoline-1-oxide (4-NQO) 26.70 44.4 nM 6.50E-200 DNA damage response 4 1.07E-157
2 SGTC_247 mechlorethamine 5.82 29.7 µM 2.32E-11 DNA damage response 5 2.98E-9
3 SGTC_2690 usnic acid 4.65 100.0 µM 6.91E-8 14 1.65E-6
4 SGTC_2693 st077581 4.14 40.7 µM 1.32E-6 29 1.70E-5
5 SGTC_1754 st042065 3.99 16.1 µM 3.01E-6 NEO1-PIK1 177 3.26E-5
6 SGTC_1801 st050089 3.73 28.4 µM 1.15E-5 NEO1-PIK1 131 9.42E-5
7 SGTC_2677 mitomycin C 3.50 100.0 µM 3.57E-5 DNA damage response 19 2.31E-4
8 SGTC_2369 9071472 3.39 200.0 µM 6.12E-5 30 3.55E-4
9 SGTC_2421 5690543 3.36 90.7 µM 6.84E-5 38 3.87E-4
10 SGTC_3292 9120825 3.29 59.8 µM 9.54E-5 31 5.04E-4
11 SGTC_2402 5553605 3.28 200.0 µM 9.68E-5 63 5.11E-4
12 SGTC_2304 7443016 3.17 91.4 µM 1.61E-4 47 7.64E-4
13 SGTC_2971 9092186 3.16 9.9 µM 1.64E-4 ergosterol biosynthesis 60 7.77E-4
14 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 3.13 3.7 µM 1.89E-4 178 8.67E-4
15 SGTC_265 mycophenolic acid 3.08 32.9 µM 2.32E-4 RPP1 & pyrimidine depletion 62 0.00102
16 SGTC_1804 st050076 3.08 49.1 µM 2.38E-4 54 0.00104
17 SGTC_2393 9031399 3.06 185.9 µM 2.58E-4 RNA processing & uracil transport 73 0.00111
18 SGTC_2687 chenodiol 3.02 100.0 µM 3.04E-4 67 0.00127
19 SGTC_2424 5537928 2.96 59.2 µM 3.90E-4 94 0.00155
20 SGTC_2245 6637752 2.94 200.0 µM 4.27E-4 41 0.00166

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.724 0 YML095C RAD10 hom Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein
0.692 0 YMR201C RAD14 hom Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein
0.610 0 YPL022W RAD1 hom Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein
0.542 3.29E-255 YER162C RAD4 hom Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins
0.429 2.37E-150 YEL037C RAD23 hom Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover
0.410 2.52E-136 YDR092W UBC13 hom Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus
0.309 2.08E-75 YGL087C MMS2 hom Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress
0.272 4.50E-58 YLR032W RAD5 hom DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
0.257 1.28E-51 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.247 5.47E-48 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.226 2.85E-40 YCR066W RAD18 hom E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA
0.217 5.00E-37 YDR492W IZH1 hom Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc
0.213 1.24E-35 YIL056W VHR1 hom Transcriptional activator; required for the vitamin H-responsive element (VHRE) mediated induction of VHT1 (Vitamin H transporter) and BIO5 (biotin biosynthesis intermediate transporter) in response to low biotin concentrations; VHR1 has a paralog, VHR2, that arose from the whole genome duplication
0.209 1.65E-34 YFL047W RGD2 hom GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.207 7.17E-34 YMR137C PSO2 hom Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress