YGR262C / BUD32

Protein kinase, component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR262C / BUD32

Click on Significant Values for Screen Details ID:SGTC_182|Compound:3448-4371|FD-Score:-3.71|P-value:3.83E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.61|P-value:5.51E-7 ID:SGTC_346|Compound:4513-0042|FD-Score:-3.55|P-value:7.41E-5 ID:SGTC_370|Compound:0180-0345|FD-Score:3.54|P-value:2.50E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:3.20|P-value:8.79E-4 ID:SGTC_464|Compound:bw-b 70c|FD-Score:3.96|P-value:4.68E-5 ID:SGTC_465|Compound:cgp-37157|FD-Score:3.45|P-value:3.56E-4 ID:SGTC_511|Compound:blebbistatin|FD-Score:3.96|P-value:4.69E-5 ID:SGTC_569|Compound:4130-2812|FD-Score:3.49|P-value:2.99E-4 ID:SGTC_587|Compound:k089-0003|FD-Score:-3.32|P-value:1.88E-4 ID:SGTC_656|Compound:4090-1979|FD-Score:-3.55|P-value:7.47E-5 ID:SGTC_1138|Compound:3761-0006|FD-Score:-5.38|P-value:7.15E-9 ID:SGTC_1141|Compound:3253-0389|FD-Score:-4.98|P-value:7.14E-8 ID:SGTC_1145|Compound:3546-0597|FD-Score:-3.17|P-value:3.30E-4 ID:SGTC_1149|Compound:3909-7961|FD-Score:-5.13|P-value:3.15E-8 ID:SGTC_1158|Compound:4092-0494|FD-Score:-4.54|P-value:7.79E-7 ID:SGTC_1164|Compound:k060-0068|FD-Score:-4.15|P-value:5.25E-6 ID:SGTC_1187|Compound:1391-0724|FD-Score:-5.50|P-value:3.60E-9 ID:SGTC_1208|Compound:2323-2347|FD-Score:-5.19|P-value:2.23E-8 ID:SGTC_1209|Compound:2323-2347|FD-Score:-4.99|P-value:6.94E-8 ID:SGTC_1211|Compound:1056-0033|FD-Score:-3.00|P-value:6.21E-4 ID:SGTC_1314|Compound:1222-0010|FD-Score:-3.58|P-value:6.73E-5 ID:SGTC_1728|Compound:st035614|FD-Score:-4.37|P-value:1.82E-6 ID:SGTC_1748|Compound:st037455|FD-Score:-2.92|P-value:8.38E-4 ID:SGTC_1939|Compound:st074455|FD-Score:-3.31|P-value:1.93E-4 ID:SGTC_1971|Compound:st069429|FD-Score:3.20|P-value:8.83E-4 ID:SGTC_2090|Compound:5301565|FD-Score:3.21|P-value:8.47E-4 ID:SGTC_2102|Compound:5377816|FD-Score:-3.21|P-value:2.88E-4 ID:SGTC_2306|Compound:7482397|FD-Score:-6.24|P-value:2.93E-11 ID:SGTC_2333|Compound:9017922|FD-Score:-2.94|P-value:7.74E-4 ID:SGTC_2339|Compound:9000433|FD-Score:4.33|P-value:8.93E-6 ID:SGTC_2370|Compound:9071495|FD-Score:3.32|P-value:5.60E-4 ID:SGTC_2389|Compound:9091542|FD-Score:-3.39|P-value:1.46E-4 ID:SGTC_2395|Compound:5646831|FD-Score:-3.49|P-value:9.56E-5 ID:SGTC_2561|Compound:acetosyringone|FD-Score:-2.92|P-value:8.39E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:-3.40|P-value:1.41E-4 ID:SGTC_2722|Compound:naftopidil|FD-Score:-2.87|P-value:9.86E-4 ID:SGTC_2830|Compound:7992007|FD-Score:-2.93|P-value:7.98E-4 ID:SGTC_2834|Compound:9001755|FD-Score:3.19|P-value:9.10E-4 ID:SGTC_3020|Compound:9089140|FD-Score:-3.43|P-value:1.23E-4 ID:SGTC_3075|Compound:9116269|FD-Score:3.54|P-value:2.51E-4 ID:SGTC_3143|Compound:9086658|FD-Score:-3.21|P-value:2.89E-4 ID:SGTC_3144|Compound:9087057|FD-Score:4.22|P-value:1.46E-5 ID:SGTC_182|Compound:3448-4371|FD-Score:-3.71|P-value:3.83E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.61|P-value:5.51E-7 ID:SGTC_346|Compound:4513-0042|FD-Score:-3.55|P-value:7.41E-5 ID:SGTC_370|Compound:0180-0345|FD-Score:3.54|P-value:2.50E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:3.20|P-value:8.79E-4 ID:SGTC_464|Compound:bw-b 70c|FD-Score:3.96|P-value:4.68E-5 ID:SGTC_465|Compound:cgp-37157|FD-Score:3.45|P-value:3.56E-4 ID:SGTC_511|Compound:blebbistatin|FD-Score:3.96|P-value:4.69E-5 ID:SGTC_569|Compound:4130-2812|FD-Score:3.49|P-value:2.99E-4 ID:SGTC_587|Compound:k089-0003|FD-Score:-3.32|P-value:1.88E-4 ID:SGTC_656|Compound:4090-1979|FD-Score:-3.55|P-value:7.47E-5 ID:SGTC_1138|Compound:3761-0006|FD-Score:-5.38|P-value:7.15E-9 ID:SGTC_1141|Compound:3253-0389|FD-Score:-4.98|P-value:7.14E-8 ID:SGTC_1145|Compound:3546-0597|FD-Score:-3.17|P-value:3.30E-4 ID:SGTC_1149|Compound:3909-7961|FD-Score:-5.13|P-value:3.15E-8 ID:SGTC_1158|Compound:4092-0494|FD-Score:-4.54|P-value:7.79E-7 ID:SGTC_1164|Compound:k060-0068|FD-Score:-4.15|P-value:5.25E-6 ID:SGTC_1187|Compound:1391-0724|FD-Score:-5.50|P-value:3.60E-9 ID:SGTC_1208|Compound:2323-2347|FD-Score:-5.19|P-value:2.23E-8 ID:SGTC_1209|Compound:2323-2347|FD-Score:-4.99|P-value:6.94E-8 ID:SGTC_1211|Compound:1056-0033|FD-Score:-3.00|P-value:6.21E-4 ID:SGTC_1314|Compound:1222-0010|FD-Score:-3.58|P-value:6.73E-5 ID:SGTC_1728|Compound:st035614|FD-Score:-4.37|P-value:1.82E-6 ID:SGTC_1748|Compound:st037455|FD-Score:-2.92|P-value:8.38E-4 ID:SGTC_1939|Compound:st074455|FD-Score:-3.31|P-value:1.93E-4 ID:SGTC_1971|Compound:st069429|FD-Score:3.20|P-value:8.83E-4 ID:SGTC_2090|Compound:5301565|FD-Score:3.21|P-value:8.47E-4 ID:SGTC_2102|Compound:5377816|FD-Score:-3.21|P-value:2.88E-4 ID:SGTC_2306|Compound:7482397|FD-Score:-6.24|P-value:2.93E-11 ID:SGTC_2333|Compound:9017922|FD-Score:-2.94|P-value:7.74E-4 ID:SGTC_2339|Compound:9000433|FD-Score:4.33|P-value:8.93E-6 ID:SGTC_2370|Compound:9071495|FD-Score:3.32|P-value:5.60E-4 ID:SGTC_2389|Compound:9091542|FD-Score:-3.39|P-value:1.46E-4 ID:SGTC_2395|Compound:5646831|FD-Score:-3.49|P-value:9.56E-5 ID:SGTC_2561|Compound:acetosyringone|FD-Score:-2.92|P-value:8.39E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:-3.40|P-value:1.41E-4 ID:SGTC_2722|Compound:naftopidil|FD-Score:-2.87|P-value:9.86E-4 ID:SGTC_2830|Compound:7992007|FD-Score:-2.93|P-value:7.98E-4 ID:SGTC_2834|Compound:9001755|FD-Score:3.19|P-value:9.10E-4 ID:SGTC_3020|Compound:9089140|FD-Score:-3.43|P-value:1.23E-4 ID:SGTC_3075|Compound:9116269|FD-Score:3.54|P-value:2.51E-4 ID:SGTC_3143|Compound:9086658|FD-Score:-3.21|P-value:2.89E-4 ID:SGTC_3144|Compound:9087057|FD-Score:4.22|P-value:1.46E-5

Top fitness defect scores for YGR262C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2339 9000433 4.33 200.0 µM 8.93E-6 26 7.43E-6
2 SGTC_3144 9087057 4.22 49.5 µM 1.46E-5 11 1.21E-5
3 SGTC_464 bw-b 70c 3.96 158.0 µM 4.68E-5 25 3.80E-5
4 SGTC_511 blebbistatin 3.96 171.0 µM 4.69E-5 DNA intercalators 22 3.81E-5
5 SGTC_370 0180-0345 3.54 38.7 µM 2.50E-4 DNA intercalators 20 1.99E-4
6 SGTC_3075 9116269 3.54 49.5 µM 2.51E-4 32 2.00E-4
7 SGTC_569 4130-2812 3.49 160.0 µM 2.99E-4 amide catabolism 17 2.37E-4
8 SGTC_465 cgp-37157 3.45 41.0 µM 3.56E-4 21 2.82E-4
9 SGTC_2370 9071495 3.32 200.0 µM 5.60E-4 mitochondrial processes 42 4.42E-4
10 SGTC_2090 5301565 3.21 104.5 µM 8.47E-4 TSC3-RPN4 79 6.67E-4
11 SGTC_439 amiodarone 3.20 34.4 µM 8.79E-4 NEO1-PIK1 133 6.92E-4
12 SGTC_1971 st069429 3.20 63.5 µM 8.83E-4 60S ribosome export 33 6.96E-4
13 SGTC_2834 9001755 3.19 58.4 µM 9.10E-4 69 7.17E-4
14 SGTC_3142 9086498 3.10 49.5 µM 0.00124 iron homeostasis 75 9.78E-4
15 SGTC_1715 st032160 3.09 10.2 µM 0.00127 79 0.00100
16 SGTC_1238 0333-0287 3.03 48.8 µM 0.00156 Golgi 69 0.00123
17 SGTC_2151 5563267 3.03 85.4 µM 0.00157 59 0.00123
18 SGTC_1934 st074720 2.98 32.6 µM 0.00181 108 0.00142
19 SGTC_77 0828-0286 2.93 12.5 µM 0.00216 TSC3-RPN4 98 0.00170
20 SGTC_467 ro 31-8220 2.93 72.8 µM 0.00217 calcium & mitochondrial duress 144 0.00171

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.107 5.94E-10 YOR312C RPL20B hom Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication
0.103 2.29E-9 YNL244C SUI1 het Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase
0.098 1.32E-8 YLR315W NKP2 hom Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2
0.081 2.81E-6 YNL297C MON2 hom Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins
0.079 4.17E-6 YHL006C SHU1 hom Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function
0.076 1.10E-5 YOR130C ORT1 hom Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome
0.073 2.28E-5 YER107C GLE2 hom RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1
0.073 2.58E-5 YJR140C HIR3 hom Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein
0.069 6.29E-5 YHL025W SNF6 hom Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions
0.066 1.20E-4 YJL052W TDH1 hom Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress
0.066 1.21E-4 YCL016C DCC1 hom Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance
0.066 1.43E-4 YHL028W WSC4 hom ER membrane protein involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3
0.066 1.46E-4 YBR015C MNN2 hom Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment
0.065 1.55E-4 YER003C PMI40 het Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
0.065 1.55E-4 YCR084C TUP1 hom General repressor of transcription, forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes