YGR263C / SAY1

Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR263C / SAY1

Click on Significant Values for Screen Details ID:SGTC_122|Compound:0236-0016|FD-Score:3.18|P-value:8.48E-4 ID:SGTC_132|Compound:0417-1715|FD-Score:3.27|P-value:6.09E-4 ID:SGTC_249|Compound:wiskostatin|FD-Score:-4.35|P-value:5.61E-6 ID:SGTC_252|Compound:miconazole|FD-Score:7.35|P-value:1.29E-13 ID:SGTC_373|Compound:0844-0104|FD-Score:-3.73|P-value:8.38E-5 ID:SGTC_386|Compound:1509-0011|FD-Score:-3.53|P-value:1.83E-4 ID:SGTC_628|Compound:1636-0253|FD-Score:3.43|P-value:3.52E-4 ID:SGTC_629|Compound:3448-6466|FD-Score:3.72|P-value:1.18E-4 ID:SGTC_804|Compound:1595-0054|FD-Score:3.28|P-value:5.99E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:3.61|P-value:1.78E-4 ID:SGTC_1445|Compound:4239-0156|FD-Score:-3.39|P-value:3.05E-4 ID:SGTC_1521|Compound:st012942|FD-Score:-3.24|P-value:5.15E-4 ID:SGTC_1549|Compound:4'-methoxyflavonol|FD-Score:4.15|P-value:1.99E-5 ID:SGTC_1579|Compound:chlormadinone acetate|FD-Score:-3.99|P-value:2.76E-5 ID:SGTC_1588|Compound:farnesol|FD-Score:3.93|P-value:5.01E-5 ID:SGTC_1590|Compound:st077108|FD-Score:-3.33|P-value:3.74E-4 ID:SGTC_1594|Compound:piperine|FD-Score:3.30|P-value:5.61E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.33|P-value:5.03E-4 ID:SGTC_1925|Compound:st057770|FD-Score:3.37|P-value:4.40E-4 ID:SGTC_1934|Compound:st074720|FD-Score:3.18|P-value:8.31E-4 ID:SGTC_2001|Compound:4023502|FD-Score:3.76|P-value:9.79E-5 ID:SGTC_2137|Compound:5261020|FD-Score:3.86|P-value:6.65E-5 ID:SGTC_2412|Compound:st077135|FD-Score:-3.24|P-value:5.20E-4 ID:SGTC_2417|Compound:cerulenin|FD-Score:4.23|P-value:1.41E-5 ID:SGTC_2770|Compound:toltrazuril|FD-Score:-3.15|P-value:7.22E-4 ID:SGTC_2930|Compound:9013230|FD-Score:3.53|P-value:2.39E-4 ID:SGTC_2933|Compound:9026794|FD-Score:-4.17|P-value:1.27E-5 ID:SGTC_2946|Compound:9065502|FD-Score:-3.37|P-value:3.28E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:3.18|P-value:8.48E-4 ID:SGTC_132|Compound:0417-1715|FD-Score:3.27|P-value:6.09E-4 ID:SGTC_249|Compound:wiskostatin|FD-Score:-4.35|P-value:5.61E-6 ID:SGTC_252|Compound:miconazole|FD-Score:7.35|P-value:1.29E-13 ID:SGTC_373|Compound:0844-0104|FD-Score:-3.73|P-value:8.38E-5 ID:SGTC_386|Compound:1509-0011|FD-Score:-3.53|P-value:1.83E-4 ID:SGTC_628|Compound:1636-0253|FD-Score:3.43|P-value:3.52E-4 ID:SGTC_629|Compound:3448-6466|FD-Score:3.72|P-value:1.18E-4 ID:SGTC_804|Compound:1595-0054|FD-Score:3.28|P-value:5.99E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:3.61|P-value:1.78E-4 ID:SGTC_1445|Compound:4239-0156|FD-Score:-3.39|P-value:3.05E-4 ID:SGTC_1521|Compound:st012942|FD-Score:-3.24|P-value:5.15E-4 ID:SGTC_1549|Compound:4'-methoxyflavonol|FD-Score:4.15|P-value:1.99E-5 ID:SGTC_1579|Compound:chlormadinone acetate|FD-Score:-3.99|P-value:2.76E-5 ID:SGTC_1588|Compound:farnesol|FD-Score:3.93|P-value:5.01E-5 ID:SGTC_1590|Compound:st077108|FD-Score:-3.33|P-value:3.74E-4 ID:SGTC_1594|Compound:piperine|FD-Score:3.30|P-value:5.61E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.33|P-value:5.03E-4 ID:SGTC_1925|Compound:st057770|FD-Score:3.37|P-value:4.40E-4 ID:SGTC_1934|Compound:st074720|FD-Score:3.18|P-value:8.31E-4 ID:SGTC_2001|Compound:4023502|FD-Score:3.76|P-value:9.79E-5 ID:SGTC_2137|Compound:5261020|FD-Score:3.86|P-value:6.65E-5 ID:SGTC_2412|Compound:st077135|FD-Score:-3.24|P-value:5.20E-4 ID:SGTC_2417|Compound:cerulenin|FD-Score:4.23|P-value:1.41E-5 ID:SGTC_2770|Compound:toltrazuril|FD-Score:-3.15|P-value:7.22E-4 ID:SGTC_2930|Compound:9013230|FD-Score:3.53|P-value:2.39E-4 ID:SGTC_2933|Compound:9026794|FD-Score:-4.17|P-value:1.27E-5 ID:SGTC_2946|Compound:9065502|FD-Score:-3.37|P-value:3.28E-4

Top fitness defect scores for YGR263C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_252 miconazole 7.35 1.3 nM 1.29E-13 ergosterol depletion effects on membrane 9 9.69E-14
2 SGTC_2417 cerulenin 4.23 830.0 nM 1.41E-5 25 1.19E-5
3 SGTC_1549 4'-methoxyflavonol 4.15 74.6 µM 1.99E-5 7 1.68E-5
4 SGTC_1588 farnesol 3.93 29.9 µM 5.01E-5 10 4.27E-5
5 SGTC_2137 5261020 3.86 140.0 µM 6.65E-5 39 5.69E-5
6 SGTC_2001 4023502 3.76 57.7 µM 9.79E-5 mitochondrial processes 22 8.40E-5
7 SGTC_629 3448-6466 3.72 76.7 µM 1.18E-4 25 1.01E-4
8 SGTC_1119 parthenolide 3.61 318.5 nM 1.78E-4 TSC3-RPN4 52 1.53E-4
9 SGTC_2930 9013230 3.53 14.9 µM 2.39E-4 heme biosynthesis & mitochondrial translocase 56 2.07E-4
10 SGTC_628 1636-0253 3.43 16.0 µM 3.52E-4 sphingolipid biosynthesis & PDR1 51 3.06E-4
11 SGTC_1925 st057770 3.37 10.1 µM 4.40E-4 TSC3-RPN4 35 3.83E-4
12 SGTC_1597 7-benzyloxygramine 3.33 47.5 µM 5.03E-4 calcium & mitochondrial duress 180 4.38E-4
13 SGTC_1594 piperine 3.30 70.1 µM 5.61E-4 36 4.89E-4
14 SGTC_804 1595-0054 3.28 20.9 µM 5.99E-4 sphingolipid biosynthesis & PDR1 67 5.23E-4
15 SGTC_132 0417-1715 3.27 25.8 µM 6.09E-4 RPP1 & pyrimidine depletion 40 5.32E-4
16 SGTC_1934 st074720 3.18 32.6 µM 8.31E-4 97 7.29E-4
17 SGTC_122 0236-0016 3.18 402.4 µM 8.48E-4 RNA processing & uracil transport 80 7.43E-4
18 SGTC_3258 9138477 3.11 49.5 µM 0.00105 27 9.25E-4
19 SGTC_843 0310-0051 3.08 143.0 µM 0.00118 46 0.00104
20 SGTC_944 3370-0224 3.04 231.0 µM 0.00132 69 0.00117

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.137 1.45E-15 YDL196W_d YDL196W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
0.125 3.12E-13 YFL005W SEC4 het Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane
0.123 8.50E-13 YOL103W ITR2 hom Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively
0.116 1.53E-11 YBR102C EXO84 het Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
0.109 2.96E-10 YAL038W CDC19 het Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
0.104 1.62E-9 YDR452W PPN1 hom Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress
0.103 2.45E-9 YGR253C PUP2 het Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
0.096 2.38E-8 YMR241W YHM2 hom Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome
0.088 3.68E-7 YLR374C_d YLR374C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W
0.085 9.38E-7 YMR026C PEX12 hom C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder
0.083 1.36E-6 YLR114C AVL9 hom Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.081 2.83E-6 YGL233W SEC15 het Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase
0.080 3.18E-6 YBR285W_p YBR285W_p hom Putative protein of unknown function; YBR285W is not an essential gene
0.080 3.55E-6 YNL071W LAT1 hom Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA
0.079 4.55E-6 YIL105C SLM1 hom Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication