YGR271C-A / EFG1

Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR271C-A / EFG1

Click on Significant Values for Screen Details ID:SGTC_47|Compound:0149-0135|FD-Score:4.48|P-value:2.11E-5 ID:SGTC_54|Compound:0159-0055|FD-Score:-3.63|P-value:2.59E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:4.33|P-value:3.89E-5 ID:SGTC_118|Compound:0083-0097|FD-Score:3.47|P-value:8.18E-4 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:4.34|P-value:3.67E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:-5.09|P-value:6.99E-7 ID:SGTC_595|Compound:3909-8003|FD-Score:4.14|P-value:8.09E-5 ID:SGTC_612|Compound:0302-0167|FD-Score:3.89|P-value:1.99E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:3.53|P-value:6.77E-4 ID:SGTC_708|Compound:3448-5400|FD-Score:3.90|P-value:1.94E-4 ID:SGTC_735|Compound:3076-0345|FD-Score:3.45|P-value:8.77E-4 ID:SGTC_745|Compound:0469-0705|FD-Score:3.66|P-value:4.43E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:3.58|P-value:5.69E-4 ID:SGTC_830|Compound:4076-0286|FD-Score:3.41|P-value:9.99E-4 ID:SGTC_831|Compound:k061-1017|FD-Score:4.66|P-value:1.03E-5 ID:SGTC_832|Compound:4083-0019|FD-Score:3.99|P-value:1.37E-4 ID:SGTC_856|Compound:0433-0016|FD-Score:3.71|P-value:3.68E-4 ID:SGTC_886|Compound:0866-0150|FD-Score:4.25|P-value:5.22E-5 ID:SGTC_897|Compound:1000-0139|FD-Score:-4.74|P-value:3.30E-6 ID:SGTC_906|Compound:1165-0455|FD-Score:4.17|P-value:7.05E-5 ID:SGTC_1013|Compound:4112-3890|FD-Score:3.67|P-value:4.19E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:3.89|P-value:2.00E-4 ID:SGTC_1189|Compound:1431-2119|FD-Score:4.81|P-value:5.36E-6 ID:SGTC_1319|Compound:1306-0155|FD-Score:4.90|P-value:3.67E-6 ID:SGTC_1321|Compound:1326-2144|FD-Score:3.60|P-value:5.37E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:3.76|P-value:3.16E-4 ID:SGTC_1340|Compound:1486-1359|FD-Score:5.47|P-value:2.61E-7 ID:SGTC_1341|Compound:1486-1360|FD-Score:4.20|P-value:6.41E-5 ID:SGTC_1368|Compound:1852-0141|FD-Score:4.59|P-value:1.36E-5 ID:SGTC_1410|Compound:3909-8106|FD-Score:4.07|P-value:1.04E-4 ID:SGTC_1433|Compound:4060-0023|FD-Score:4.01|P-value:1.29E-4 ID:SGTC_1437|Compound:4092-1038|FD-Score:3.81|P-value:2.60E-4 ID:SGTC_1624|Compound:st003710|FD-Score:3.50|P-value:7.48E-4 ID:SGTC_1781|Compound:st038725|FD-Score:5.96|P-value:2.19E-8 ID:SGTC_1912|Compound:st060209|FD-Score:3.50|P-value:7.33E-4 ID:SGTC_1941|Compound:st076221|FD-Score:-4.66|P-value:4.83E-6 ID:SGTC_1952|Compound:st077036|FD-Score:3.55|P-value:6.29E-4 ID:SGTC_1959|Compound:st077091|FD-Score:3.76|P-value:3.09E-4 ID:SGTC_2225|Compound:6654152|FD-Score:3.74|P-value:3.29E-4 ID:SGTC_2296|Compound:7971656|FD-Score:5.94|P-value:2.48E-8 ID:SGTC_2337|Compound:9009472|FD-Score:-3.65|P-value:2.42E-4 ID:SGTC_2559|Compound:tetrahydrosappanone a trimethyl ether|FD-Score:3.49|P-value:7.60E-4 ID:SGTC_2658|Compound:polymyxin b sulfate|FD-Score:3.78|P-value:2.95E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.70|P-value:8.48E-8 ID:SGTC_2852|Compound:9023854|FD-Score:4.59|P-value:1.37E-5 ID:SGTC_3250|Compound:9135583|FD-Score:3.83|P-value:2.45E-4 ID:SGTC_3259|Compound:9138485|FD-Score:4.37|P-value:3.26E-5 ID:SGTC_3334|Compound:9142812|FD-Score:3.72|P-value:3.62E-4 ID:SGTC_3345|Compound:9148410|FD-Score:-4.17|P-value:3.52E-5 ID:SGTC_47|Compound:0149-0135|FD-Score:4.48|P-value:2.11E-5 ID:SGTC_54|Compound:0159-0055|FD-Score:-3.63|P-value:2.59E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:4.33|P-value:3.89E-5 ID:SGTC_118|Compound:0083-0097|FD-Score:3.47|P-value:8.18E-4 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:4.34|P-value:3.67E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:-5.09|P-value:6.99E-7 ID:SGTC_595|Compound:3909-8003|FD-Score:4.14|P-value:8.09E-5 ID:SGTC_612|Compound:0302-0167|FD-Score:3.89|P-value:1.99E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:3.53|P-value:6.77E-4 ID:SGTC_708|Compound:3448-5400|FD-Score:3.90|P-value:1.94E-4 ID:SGTC_735|Compound:3076-0345|FD-Score:3.45|P-value:8.77E-4 ID:SGTC_745|Compound:0469-0705|FD-Score:3.66|P-value:4.43E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:3.58|P-value:5.69E-4 ID:SGTC_830|Compound:4076-0286|FD-Score:3.41|P-value:9.99E-4 ID:SGTC_831|Compound:k061-1017|FD-Score:4.66|P-value:1.03E-5 ID:SGTC_832|Compound:4083-0019|FD-Score:3.99|P-value:1.37E-4 ID:SGTC_856|Compound:0433-0016|FD-Score:3.71|P-value:3.68E-4 ID:SGTC_886|Compound:0866-0150|FD-Score:4.25|P-value:5.22E-5 ID:SGTC_897|Compound:1000-0139|FD-Score:-4.74|P-value:3.30E-6 ID:SGTC_906|Compound:1165-0455|FD-Score:4.17|P-value:7.05E-5 ID:SGTC_1013|Compound:4112-3890|FD-Score:3.67|P-value:4.19E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:3.89|P-value:2.00E-4 ID:SGTC_1189|Compound:1431-2119|FD-Score:4.81|P-value:5.36E-6 ID:SGTC_1319|Compound:1306-0155|FD-Score:4.90|P-value:3.67E-6 ID:SGTC_1321|Compound:1326-2144|FD-Score:3.60|P-value:5.37E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:3.76|P-value:3.16E-4 ID:SGTC_1340|Compound:1486-1359|FD-Score:5.47|P-value:2.61E-7 ID:SGTC_1341|Compound:1486-1360|FD-Score:4.20|P-value:6.41E-5 ID:SGTC_1368|Compound:1852-0141|FD-Score:4.59|P-value:1.36E-5 ID:SGTC_1410|Compound:3909-8106|FD-Score:4.07|P-value:1.04E-4 ID:SGTC_1433|Compound:4060-0023|FD-Score:4.01|P-value:1.29E-4 ID:SGTC_1437|Compound:4092-1038|FD-Score:3.81|P-value:2.60E-4 ID:SGTC_1624|Compound:st003710|FD-Score:3.50|P-value:7.48E-4 ID:SGTC_1781|Compound:st038725|FD-Score:5.96|P-value:2.19E-8 ID:SGTC_1912|Compound:st060209|FD-Score:3.50|P-value:7.33E-4 ID:SGTC_1941|Compound:st076221|FD-Score:-4.66|P-value:4.83E-6 ID:SGTC_1952|Compound:st077036|FD-Score:3.55|P-value:6.29E-4 ID:SGTC_1959|Compound:st077091|FD-Score:3.76|P-value:3.09E-4 ID:SGTC_2225|Compound:6654152|FD-Score:3.74|P-value:3.29E-4 ID:SGTC_2296|Compound:7971656|FD-Score:5.94|P-value:2.48E-8 ID:SGTC_2337|Compound:9009472|FD-Score:-3.65|P-value:2.42E-4 ID:SGTC_2559|Compound:tetrahydrosappanone a trimethyl ether|FD-Score:3.49|P-value:7.60E-4 ID:SGTC_2658|Compound:polymyxin b sulfate|FD-Score:3.78|P-value:2.95E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.70|P-value:8.48E-8 ID:SGTC_2852|Compound:9023854|FD-Score:4.59|P-value:1.37E-5 ID:SGTC_3250|Compound:9135583|FD-Score:3.83|P-value:2.45E-4 ID:SGTC_3259|Compound:9138485|FD-Score:4.37|P-value:3.26E-5 ID:SGTC_3334|Compound:9142812|FD-Score:3.72|P-value:3.62E-4 ID:SGTC_3345|Compound:9148410|FD-Score:-4.17|P-value:3.52E-5

Top fitness defect scores for YGR271C-A deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1781 st038725 5.96 66.3 µM 2.19E-8 NEO1 57 1.23E-9
2 SGTC_2296 7971656 5.94 126.5 µM 2.48E-8 12 1.42E-9
3 SGTC_2688 kanamycin b 5.70 96.7 µM 8.48E-8 calcium & mitochondrial duress 180 5.95E-9
4 SGTC_1340 1486-1359 5.47 257.0 µM 2.61E-7 4 2.21E-8
5 SGTC_1319 1306-0155 4.90 43.7 µM 3.67E-6 8 4.78E-7
6 SGTC_1189 1431-2119 4.81 7.3 µM 5.36E-6 9 7.42E-7
7 SGTC_831 k061-1017 4.66 217.0 µM 1.03E-5 translation 30 1.59E-6
8 SGTC_1368 1852-0141 4.59 284.0 µM 1.36E-5 ERG2 26 2.21E-6
9 SGTC_2852 9023854 4.59 52.0 µM 1.37E-5 19 2.22E-6
10 SGTC_47 0149-0135 4.48 24.2 µM 2.11E-5 TSC3-RPN4 13 3.66E-6
11 SGTC_3259 9138485 4.37 49.5 µM 3.26E-5 7 6.09E-6
12 SGTC_243 5hr heat shock (37°C) + splitomicin 4.34 1.0 µM 3.67E-5 heat shock/prefoldin 40 6.98E-6
13 SGTC_59 0330-0160 4.33 1.3 µM 3.89E-5 93 7.49E-6
14 SGTC_886 0866-0150 4.25 153.0 µM 5.22E-5 17 1.05E-5
15 SGTC_1341 1486-1360 4.20 280.0 µM 6.41E-5 17 1.34E-5
16 SGTC_906 1165-0455 4.17 526.0 µM 7.05E-5 8 1.50E-5
17 SGTC_595 3909-8003 4.14 73.7 µM 8.09E-5 9 1.76E-5
18 SGTC_1410 3909-8106 4.07 77.9 µM 1.04E-4 10 2.36E-5
19 SGTC_1433 4060-0023 4.01 7.6 µM 1.29E-4 tubulin folding & SWR complex 17 3.04E-5
20 SGTC_832 4083-0019 3.99 164.0 µM 1.37E-4 translation 92 3.24E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.200 9.36E-32 YLR048W RPS0B hom Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.186 1.67E-27 YJL136C RPS21B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication
0.186 1.82E-27 YDR140W MTQ2 hom S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC
0.179 1.57E-25 YGL246C RAI1 hom Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z
0.173 7.15E-24 YGR162W TIF4631 hom Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication
0.159 2.60E-20 YLR068W FYV7 hom Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin
0.158 3.43E-20 YOR085W OST3 hom Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.155 1.79E-19 YIR026C YVH1 hom Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases
0.154 3.50E-19 YJL188C_d BUD19_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay
0.152 9.44E-19 YJL189W RPL39 hom Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog
0.145 3.87E-17 YEL054C RPL12A hom Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication
0.140 3.63E-16 YOR295W UAF30 hom Subunit of UAF (upstream activation factor); UAF is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; deletion decreases cellular growth rate; UAF30 has a paralog, TRI1, that arose from the whole genome duplication
0.135 4.00E-15 YLR287C-A RPS30A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication
0.134 7.17E-15 YPL239W YAR1 hom Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock
0.133 1.14E-14 YOR309C_d YOR309C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58