YHL001W / RPL14B

Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YHL001W / RPL14B

Click on Significant Values for Screen Details ID:SGTC_53|Compound:0973-0023|FD-Score:3.16|P-value:6.84E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-7.11|P-value:6.00E-13 ID:SGTC_137|Compound:0438-0332|FD-Score:-3.77|P-value:8.77E-5 ID:SGTC_196|Compound:k015-0023|FD-Score:3.19|P-value:6.33E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:3.18|P-value:6.44E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:3.07|P-value:9.35E-4 ID:SGTC_567|Compound:1080-0542|FD-Score:3.27|P-value:4.69E-4 ID:SGTC_571|Compound:k831-0106|FD-Score:3.14|P-value:7.46E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:-3.20|P-value:7.44E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:3.15|P-value:7.08E-4 ID:SGTC_884|Compound:0851-0618|FD-Score:-3.28|P-value:5.47E-4 ID:SGTC_923|Compound:1900-2434|FD-Score:3.24|P-value:5.23E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:-4.80|P-value:8.39E-7 ID:SGTC_947|Compound:3447-0010|FD-Score:-5.93|P-value:1.66E-9 ID:SGTC_1159|Compound:4092-0538|FD-Score:3.54|P-value:1.73E-4 ID:SGTC_1252|Compound:0493-0875|FD-Score:3.13|P-value:7.77E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:3.32|P-value:3.87E-4 ID:SGTC_1313|Compound:1220-0047|FD-Score:3.64|P-value:1.19E-4 ID:SGTC_1325|Compound:1431-0015|FD-Score:-3.22|P-value:6.95E-4 ID:SGTC_1329|Compound:1447-1697|FD-Score:-3.19|P-value:7.55E-4 ID:SGTC_1499|Compound:4480-0877|FD-Score:3.16|P-value:6.94E-4 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:4.73|P-value:9.09E-7 ID:SGTC_1654|Compound:st012436|FD-Score:3.19|P-value:6.18E-4 ID:SGTC_1941|Compound:st076221|FD-Score:-5.23|P-value:9.26E-8 ID:SGTC_1949|Compound:st076024|FD-Score:-3.40|P-value:3.56E-4 ID:SGTC_1966|Compound:st070145|FD-Score:3.60|P-value:1.37E-4 ID:SGTC_2007|Compound:4011539|FD-Score:-3.95|P-value:4.22E-5 ID:SGTC_2012|Compound:4023501|FD-Score:-3.25|P-value:6.24E-4 ID:SGTC_2111|Compound:5486399|FD-Score:-3.60|P-value:1.70E-4 ID:SGTC_2129|Compound:girgensonine|FD-Score:3.84|P-value:5.19E-5 ID:SGTC_2207|Compound:7258803|FD-Score:3.08|P-value:9.10E-4 ID:SGTC_2395|Compound:5646831|FD-Score:3.23|P-value:5.38E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:-5.62|P-value:1.02E-8 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-3.30|P-value:5.10E-4 ID:SGTC_2445|Compound:5742597|FD-Score:3.71|P-value:8.79E-5 ID:SGTC_2476|Compound:5806916|FD-Score:4.18|P-value:1.21E-5 ID:SGTC_2484|Compound:5809818|FD-Score:-3.63|P-value:1.52E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.36|P-value:4.18E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-3.22|P-value:6.84E-4 ID:SGTC_2665|Compound:lovastatin|FD-Score:3.31|P-value:4.05E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:-3.45|P-value:2.99E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:4.35|P-value:5.76E-6 ID:SGTC_2764|Compound:methyldopa|FD-Score:3.66|P-value:1.09E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:-4.04|P-value:2.83E-5 ID:SGTC_2819|Compound:7996655|FD-Score:3.51|P-value:1.95E-4 ID:SGTC_2941|Compound:9056145|FD-Score:3.86|P-value:4.88E-5 ID:SGTC_2974|Compound:9094440|FD-Score:-3.81|P-value:7.37E-5 ID:SGTC_3033|Compound:9090887|FD-Score:3.18|P-value:6.50E-4 ID:SGTC_3100|Compound:9120536|FD-Score:3.09|P-value:8.98E-4 ID:SGTC_3152|Compound:9099253|FD-Score:-3.79|P-value:7.97E-5 ID:SGTC_3165|Compound:9101544|FD-Score:-3.23|P-value:6.72E-4 ID:SGTC_3174|Compound:9107204|FD-Score:-4.02|P-value:3.09E-5 ID:SGTC_53|Compound:0973-0023|FD-Score:3.16|P-value:6.84E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-7.11|P-value:6.00E-13 ID:SGTC_137|Compound:0438-0332|FD-Score:-3.77|P-value:8.77E-5 ID:SGTC_196|Compound:k015-0023|FD-Score:3.19|P-value:6.33E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:3.18|P-value:6.44E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:3.07|P-value:9.35E-4 ID:SGTC_567|Compound:1080-0542|FD-Score:3.27|P-value:4.69E-4 ID:SGTC_571|Compound:k831-0106|FD-Score:3.14|P-value:7.46E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:-3.20|P-value:7.44E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:3.15|P-value:7.08E-4 ID:SGTC_884|Compound:0851-0618|FD-Score:-3.28|P-value:5.47E-4 ID:SGTC_923|Compound:1900-2434|FD-Score:3.24|P-value:5.23E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:-4.80|P-value:8.39E-7 ID:SGTC_947|Compound:3447-0010|FD-Score:-5.93|P-value:1.66E-9 ID:SGTC_1159|Compound:4092-0538|FD-Score:3.54|P-value:1.73E-4 ID:SGTC_1252|Compound:0493-0875|FD-Score:3.13|P-value:7.77E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:3.32|P-value:3.87E-4 ID:SGTC_1313|Compound:1220-0047|FD-Score:3.64|P-value:1.19E-4 ID:SGTC_1325|Compound:1431-0015|FD-Score:-3.22|P-value:6.95E-4 ID:SGTC_1329|Compound:1447-1697|FD-Score:-3.19|P-value:7.55E-4 ID:SGTC_1499|Compound:4480-0877|FD-Score:3.16|P-value:6.94E-4 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:4.73|P-value:9.09E-7 ID:SGTC_1654|Compound:st012436|FD-Score:3.19|P-value:6.18E-4 ID:SGTC_1941|Compound:st076221|FD-Score:-5.23|P-value:9.26E-8 ID:SGTC_1949|Compound:st076024|FD-Score:-3.40|P-value:3.56E-4 ID:SGTC_1966|Compound:st070145|FD-Score:3.60|P-value:1.37E-4 ID:SGTC_2007|Compound:4011539|FD-Score:-3.95|P-value:4.22E-5 ID:SGTC_2012|Compound:4023501|FD-Score:-3.25|P-value:6.24E-4 ID:SGTC_2111|Compound:5486399|FD-Score:-3.60|P-value:1.70E-4 ID:SGTC_2129|Compound:girgensonine|FD-Score:3.84|P-value:5.19E-5 ID:SGTC_2207|Compound:7258803|FD-Score:3.08|P-value:9.10E-4 ID:SGTC_2395|Compound:5646831|FD-Score:3.23|P-value:5.38E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:-5.62|P-value:1.02E-8 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-3.30|P-value:5.10E-4 ID:SGTC_2445|Compound:5742597|FD-Score:3.71|P-value:8.79E-5 ID:SGTC_2476|Compound:5806916|FD-Score:4.18|P-value:1.21E-5 ID:SGTC_2484|Compound:5809818|FD-Score:-3.63|P-value:1.52E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.36|P-value:4.18E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-3.22|P-value:6.84E-4 ID:SGTC_2665|Compound:lovastatin|FD-Score:3.31|P-value:4.05E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:-3.45|P-value:2.99E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:4.35|P-value:5.76E-6 ID:SGTC_2764|Compound:methyldopa|FD-Score:3.66|P-value:1.09E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:-4.04|P-value:2.83E-5 ID:SGTC_2819|Compound:7996655|FD-Score:3.51|P-value:1.95E-4 ID:SGTC_2941|Compound:9056145|FD-Score:3.86|P-value:4.88E-5 ID:SGTC_2974|Compound:9094440|FD-Score:-3.81|P-value:7.37E-5 ID:SGTC_3033|Compound:9090887|FD-Score:3.18|P-value:6.50E-4 ID:SGTC_3100|Compound:9120536|FD-Score:3.09|P-value:8.98E-4 ID:SGTC_3152|Compound:9099253|FD-Score:-3.79|P-value:7.97E-5 ID:SGTC_3165|Compound:9101544|FD-Score:-3.23|P-value:6.72E-4 ID:SGTC_3174|Compound:9107204|FD-Score:-4.02|P-value:3.09E-5

Top fitness defect scores for YHL001W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1589 2',5'-dimethoxyflavone 4.73 47.2 µM 9.09E-7 5 1.12E-6
2 SGTC_2754 granisetron 4.35 64.0 µM 5.76E-6 25 6.94E-6
3 SGTC_2476 5806916 4.18 10.5 µM 1.21E-5 11 1.45E-5
4 SGTC_2941 9056145 3.86 11.2 µM 4.88E-5 21 5.73E-5
5 SGTC_2129 girgensonine 3.84 121.0 µM 5.19E-5 heme biosynthesis & mitochondrial translocase 35 6.10E-5
6 SGTC_2445 5742597 3.71 35.6 µM 8.79E-5 41 1.03E-4
7 SGTC_2764 methyldopa 3.66 94.7 µM 1.09E-4 22 1.27E-4
8 SGTC_1313 1220-0047 3.64 237.0 µM 1.19E-4 52 1.38E-4
9 SGTC_1966 st070145 3.60 16.1 µM 1.37E-4 31 1.59E-4
10 SGTC_1159 4092-0538 3.54 61.2 µM 1.73E-4 45 2.00E-4
11 SGTC_2819 7996655 3.51 46.4 µM 1.95E-4 32 2.25E-4
12 SGTC_1261 0712-0010 3.32 12.7 µM 3.87E-4 RNA processing & uracil transport 49 4.43E-4
13 SGTC_2665 lovastatin 3.31 100.0 µM 4.05E-4 32 4.64E-4
14 SGTC_567 1080-0542 3.27 68.4 µM 4.69E-4 35 5.36E-4
15 SGTC_923 1900-2434 3.24 73.3 µM 5.23E-4 RNA processing & uracil transport 54 5.96E-4
16 SGTC_2395 5646831 3.23 180.2 µM 5.38E-4 58 6.14E-4
17 SGTC_1654 st012436 3.19 41.8 µM 6.18E-4 36 7.04E-4
18 SGTC_196 k015-0023 3.19 18.1 µM 6.33E-4 PDR1 48 7.21E-4
19 SGTC_212 3807-4682 3.18 38.8 µM 6.44E-4 tubulin folding & SWR complex 123 7.33E-4
20 SGTC_3033 9090887 3.18 49.5 µM 6.50E-4 fatty acid desaturase (OLE1) 65 7.40E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.124 5.49E-13 YDR156W RPA14 hom RNA polymerase I subunit A14
0.119 4.39E-12 YDL236W PHO13 hom Alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity
0.119 5.00E-12 YDL240W LRG1 hom Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis
0.115 2.26E-11 YDL136W RPL35B hom Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication
0.112 8.49E-11 YOL079W_d YOL079W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.097 1.70E-8 YOR234C RPL33B hom Ribosomal 60S subunit protein L33B; rpl33b null mutant exhibits normal growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33B has a paralog, RPL33A, that arose from the whole genome duplication
0.097 1.89E-8 YPR106W ISR1 hom Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C
0.094 4.68E-8 YNL067W RPL9B hom Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6
0.093 6.92E-8 YNL001W DOM34 hom Protein that facilitates ribosomal subunit dissociation; acts when translation is stalled, with binding partner Hbs1p; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication
0.093 7.54E-8 YOR077W RTS2 het Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication
0.087 5.26E-7 YOL158C ENB1 hom Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment
0.086 6.13E-7 YBR249C ARO4 hom 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress
0.085 7.74E-7 YML058W SML1 hom Ribonucleotide reductase inhibitor involved in regulating dNTP production; regulated by Mec1p and Rad53p during DNA damage and S phase
0.084 1.12E-6 YDR443C SSN2 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation
0.084 1.25E-6 YGR206W MVB12 hom ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin