HIPHOP chemogenomics database

YHL002W / HSE1

Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes

Zygosity: Homozygous strain

fixedexpanded
Profile for YHL002W / HSE1

Click on Significant Values for Screen Details

Top fitness defect scores for YHL002W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1712	st032288	6.92	53.4 µM	2.32E-11	calcium & mitochondrial duress	56	2.25E-12
2	SGTC_6	0844-0013	5.79	19.8 µM	1.78E-8		18	3.43E-9
3	SGTC_1687	st020778	5.63	55.4 µM	4.23E-8		117	8.89E-9
4	SGTC_2755	lomerizine	5.44	42.7 µM	1.17E-7		6	2.72E-8
5	SGTC_1842	st055362	4.77	61.2 µM	2.82E-6		5	9.06E-7
6	SGTC_432	NS 1619	4.76	61.3 µM	3.05E-6		12	9.88E-7
7	SGTC_131	0141-0277	4.69	33.9 µM	4.17E-6		20	1.39E-6
8	SGTC_927	2425-1185	4.61	123.0 µM	5.70E-6		18	1.97E-6
9	SGTC_1030	k029-0042	4.57	36.7 µM	6.89E-6	TSC3-RPN4	13	2.42E-6
10	SGTC_2688	kanamycin b	4.56	96.7 µM	7.32E-6	calcium & mitochondrial duress	290	2.59E-6
11	SGTC_1042	0350-0096	4.51	82.0 µM	8.97E-6	TSC3-RPN4	12	3.24E-6
12	SGTC_1283	0922-0125	4.40	16.4 µM	1.43E-5		14	5.42E-6
13	SGTC_2021	4019055	4.28	173.0 µM	2.37E-5	endomembrane recycling	103	9.46E-6
14	SGTC_1120	ficusin	4.05	1.8 µM	5.85E-5		22	2.55E-5
15	SGTC_1831	st055328	3.98	12.0 µM	7.64E-5	heme biosynthesis & mitochondrial translocase	23	3.42E-5
16	SGTC_1495	4469-0029	3.95	237.0 µM	8.52E-5		107	3.86E-5
17	SGTC_636	0kpi-0074	3.94	126.0 µM	8.83E-5		19	4.01E-5
18	SGTC_1914	st062367	3.81	72.6 µM	1.47E-4		24	7.00E-5
19	SGTC_2494	18-aminoabieta-8,11,13-triene sulfate	3.74	3.7 µM	1.87E-4		102	9.17E-5
20	SGTC_1033	k072-0102	3.70	21.3 µM	2.18E-4	60S ribosome export	30	1.08E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.230	2.00E-41	YDR466W	PKH3	hom	Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.222	9.87E-39	YDR467C_d	YDR467C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.217	5.18E-37	YKL051W	SFK1	hom	Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.215	2.58E-36	YDL019C	OSH2	hom	Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.210	1.26E-34	YGL203C	KEX1	hom	Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins
0.193	2.15E-29	YGR206W	MVB12	hom	ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
0.192	2.43E-29	YJL164C	TPK1	hom	cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication
0.192	2.79E-29	YGR261C	APL6	hom	Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools
0.186	1.45E-27	YDR475C	JIP4	hom	Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.185	3.20E-27	YAL002W	VPS8	hom	Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif
0.183	1.36E-26	YPL120W	VPS30	hom	Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1
0.171	2.13E-23	YDR357C_p	CNL1_p	hom	Protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.169	6.07E-23	YBL051C	PIN4	hom	Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage
0.168	8.85E-23	YPL145C	KES1	hom	One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication
0.167	2.18E-22	YEL022W	GEA2	hom	Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication