YHL007C / STE20

Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p

Zygosity: Homozygous strain
fixedexpanded
Profile for YHL007C / STE20

Click on Significant Values for Screen Details ID:SGTC_15|Compound:0266-0086|FD-Score:4.53|P-value:3.88E-6 ID:SGTC_22|Compound:0964-0034|FD-Score:-3.17|P-value:8.03E-4 ID:SGTC_24|Compound:0417-1663|FD-Score:3.45|P-value:3.41E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:6.80|P-value:9.28E-12 ID:SGTC_138|Compound:0850-0259|FD-Score:3.73|P-value:1.20E-4 ID:SGTC_206|Compound:4546-0025|FD-Score:3.46|P-value:3.20E-4 ID:SGTC_230|Compound:MPP+|FD-Score:4.66|P-value:2.18E-6 ID:SGTC_296|Compound:1222-0039|FD-Score:3.17|P-value:8.88E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:12.30|P-value:2.20E-34 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:3.85|P-value:7.35E-5 ID:SGTC_514|Compound:4466-0038|FD-Score:6.81|P-value:8.64E-12 ID:SGTC_736|Compound:0958-0254|FD-Score:3.39|P-value:4.12E-4 ID:SGTC_787|Compound:4152-0075|FD-Score:-3.18|P-value:7.93E-4 ID:SGTC_887|Compound:0885-0007|FD-Score:3.50|P-value:2.85E-4 ID:SGTC_1151|Compound:3909-8316|FD-Score:3.45|P-value:3.41E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:3.75|P-value:1.09E-4 ID:SGTC_1375|Compound:2910-0797|FD-Score:3.70|P-value:1.35E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:3.41|P-value:3.93E-4 ID:SGTC_1383|Compound:3049-0032|FD-Score:3.30|P-value:5.77E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:3.47|P-value:3.13E-4 ID:SGTC_1518|Compound:st038567|FD-Score:3.21|P-value:7.78E-4 ID:SGTC_1755|Compound:st041960|FD-Score:-4.43|P-value:5.35E-6 ID:SGTC_1762|Compound:st045503|FD-Score:-3.34|P-value:4.51E-4 ID:SGTC_1776|Compound:st049249|FD-Score:-4.09|P-value:2.48E-5 ID:SGTC_1792|Compound:st045773|FD-Score:4.13|P-value:2.36E-5 ID:SGTC_1963|Compound:st076565|FD-Score:3.22|P-value:7.58E-4 ID:SGTC_2012|Compound:4023501|FD-Score:-3.74|P-value:1.03E-4 ID:SGTC_2034|Compound:5197015|FD-Score:3.96|P-value:4.66E-5 ID:SGTC_2038|Compound:4100038|FD-Score:3.43|P-value:3.60E-4 ID:SGTC_2045|Compound:5106652|FD-Score:4.63|P-value:2.44E-6 ID:SGTC_2123|Compound:5259487|FD-Score:3.34|P-value:5.08E-4 ID:SGTC_2163|Compound:5671233|FD-Score:3.46|P-value:3.23E-4 ID:SGTC_2180|Compound:6301957|FD-Score:3.95|P-value:5.02E-5 ID:SGTC_2242|Compound:6630449|FD-Score:-3.52|P-value:2.37E-4 ID:SGTC_2261|Compound:9010538|FD-Score:3.62|P-value:1.79E-4 ID:SGTC_2310|Compound:7668677|FD-Score:3.42|P-value:3.82E-4 ID:SGTC_2328|Compound:6201408|FD-Score:-3.33|P-value:4.60E-4 ID:SGTC_2365|Compound:9042475|FD-Score:4.20|P-value:1.70E-5 ID:SGTC_2415|Compound:st077333|FD-Score:3.76|P-value:1.07E-4 ID:SGTC_2468|Compound:5987765|FD-Score:-3.33|P-value:4.63E-4 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:3.38|P-value:4.36E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:3.82|P-value:8.45E-5 ID:SGTC_2858|Compound:9025781|FD-Score:-3.64|P-value:1.47E-4 ID:SGTC_2977|Compound:9011197|FD-Score:4.26|P-value:1.32E-5 ID:SGTC_2978|Compound:9011784|FD-Score:-3.36|P-value:4.27E-4 ID:SGTC_2986|Compound:9022445|FD-Score:5.92|P-value:2.51E-9 ID:SGTC_3031|Compound:9088762|FD-Score:3.46|P-value:3.29E-4 ID:SGTC_3120|Compound:9124077|FD-Score:3.93|P-value:5.44E-5 ID:SGTC_3128|Compound:9127691|FD-Score:-3.26|P-value:5.92E-4 ID:SGTC_3139|Compound:9095020|FD-Score:3.40|P-value:4.06E-4 ID:SGTC_3221|Compound:9128209|FD-Score:-3.62|P-value:1.61E-4 ID:SGTC_3222|Compound:9128301|FD-Score:-4.24|P-value:1.30E-5 ID:SGTC_3235|Compound:9132602|FD-Score:6.10|P-value:8.59E-10 ID:SGTC_3329|Compound:9141233|FD-Score:-3.47|P-value:2.87E-4 ID:SGTC_3334|Compound:9142812|FD-Score:5.15|P-value:1.90E-7 ID:SGTC_15|Compound:0266-0086|FD-Score:4.53|P-value:3.88E-6 ID:SGTC_22|Compound:0964-0034|FD-Score:-3.17|P-value:8.03E-4 ID:SGTC_24|Compound:0417-1663|FD-Score:3.45|P-value:3.41E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:6.80|P-value:9.28E-12 ID:SGTC_138|Compound:0850-0259|FD-Score:3.73|P-value:1.20E-4 ID:SGTC_206|Compound:4546-0025|FD-Score:3.46|P-value:3.20E-4 ID:SGTC_230|Compound:MPP+|FD-Score:4.66|P-value:2.18E-6 ID:SGTC_296|Compound:1222-0039|FD-Score:3.17|P-value:8.88E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:12.30|P-value:2.20E-34 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:3.85|P-value:7.35E-5 ID:SGTC_514|Compound:4466-0038|FD-Score:6.81|P-value:8.64E-12 ID:SGTC_736|Compound:0958-0254|FD-Score:3.39|P-value:4.12E-4 ID:SGTC_787|Compound:4152-0075|FD-Score:-3.18|P-value:7.93E-4 ID:SGTC_887|Compound:0885-0007|FD-Score:3.50|P-value:2.85E-4 ID:SGTC_1151|Compound:3909-8316|FD-Score:3.45|P-value:3.41E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:3.75|P-value:1.09E-4 ID:SGTC_1375|Compound:2910-0797|FD-Score:3.70|P-value:1.35E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:3.41|P-value:3.93E-4 ID:SGTC_1383|Compound:3049-0032|FD-Score:3.30|P-value:5.77E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:3.47|P-value:3.13E-4 ID:SGTC_1518|Compound:st038567|FD-Score:3.21|P-value:7.78E-4 ID:SGTC_1755|Compound:st041960|FD-Score:-4.43|P-value:5.35E-6 ID:SGTC_1762|Compound:st045503|FD-Score:-3.34|P-value:4.51E-4 ID:SGTC_1776|Compound:st049249|FD-Score:-4.09|P-value:2.48E-5 ID:SGTC_1792|Compound:st045773|FD-Score:4.13|P-value:2.36E-5 ID:SGTC_1963|Compound:st076565|FD-Score:3.22|P-value:7.58E-4 ID:SGTC_2012|Compound:4023501|FD-Score:-3.74|P-value:1.03E-4 ID:SGTC_2034|Compound:5197015|FD-Score:3.96|P-value:4.66E-5 ID:SGTC_2038|Compound:4100038|FD-Score:3.43|P-value:3.60E-4 ID:SGTC_2045|Compound:5106652|FD-Score:4.63|P-value:2.44E-6 ID:SGTC_2123|Compound:5259487|FD-Score:3.34|P-value:5.08E-4 ID:SGTC_2163|Compound:5671233|FD-Score:3.46|P-value:3.23E-4 ID:SGTC_2180|Compound:6301957|FD-Score:3.95|P-value:5.02E-5 ID:SGTC_2242|Compound:6630449|FD-Score:-3.52|P-value:2.37E-4 ID:SGTC_2261|Compound:9010538|FD-Score:3.62|P-value:1.79E-4 ID:SGTC_2310|Compound:7668677|FD-Score:3.42|P-value:3.82E-4 ID:SGTC_2328|Compound:6201408|FD-Score:-3.33|P-value:4.60E-4 ID:SGTC_2365|Compound:9042475|FD-Score:4.20|P-value:1.70E-5 ID:SGTC_2415|Compound:st077333|FD-Score:3.76|P-value:1.07E-4 ID:SGTC_2468|Compound:5987765|FD-Score:-3.33|P-value:4.63E-4 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:3.38|P-value:4.36E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:3.82|P-value:8.45E-5 ID:SGTC_2858|Compound:9025781|FD-Score:-3.64|P-value:1.47E-4 ID:SGTC_2977|Compound:9011197|FD-Score:4.26|P-value:1.32E-5 ID:SGTC_2978|Compound:9011784|FD-Score:-3.36|P-value:4.27E-4 ID:SGTC_2986|Compound:9022445|FD-Score:5.92|P-value:2.51E-9 ID:SGTC_3031|Compound:9088762|FD-Score:3.46|P-value:3.29E-4 ID:SGTC_3120|Compound:9124077|FD-Score:3.93|P-value:5.44E-5 ID:SGTC_3128|Compound:9127691|FD-Score:-3.26|P-value:5.92E-4 ID:SGTC_3139|Compound:9095020|FD-Score:3.40|P-value:4.06E-4 ID:SGTC_3221|Compound:9128209|FD-Score:-3.62|P-value:1.61E-4 ID:SGTC_3222|Compound:9128301|FD-Score:-4.24|P-value:1.30E-5 ID:SGTC_3235|Compound:9132602|FD-Score:6.10|P-value:8.59E-10 ID:SGTC_3329|Compound:9141233|FD-Score:-3.47|P-value:2.87E-4 ID:SGTC_3334|Compound:9142812|FD-Score:5.15|P-value:1.90E-7

Top fitness defect scores for YHL007C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_358 0075-0058 12.30 9.4 µM 2.20E-34 1 3.77E-35
2 SGTC_514 4466-0038 6.81 2.6 nM 8.64E-12 cell wall 58 4.78E-12
3 SGTC_58 0957-0393 6.80 9.0 µM 9.28E-12 cell wall 170 5.14E-12
4 SGTC_3235 9132602 6.10 49.5 µM 8.59E-10 4 5.28E-10
5 SGTC_2986 9022445 5.92 71.4 µM 2.51E-9 4 1.58E-9
6 SGTC_3334 9142812 5.15 21.9 µM 1.90E-7 10 1.32E-7
7 SGTC_230 MPP+ 4.66 141.7 µM 2.18E-6 9 1.60E-6
8 SGTC_2045 5106652 4.63 78.0 µM 2.44E-6 5 1.80E-6
9 SGTC_15 0266-0086 4.53 27.1 µM 3.88E-6 endomembrane recycling 43 2.90E-6
10 SGTC_2977 9011197 4.26 71.4 µM 1.32E-5 13 1.01E-5
11 SGTC_2365 9042475 4.20 200.0 µM 1.70E-5 27 1.31E-5
12 SGTC_1792 st045773 4.13 43.6 µM 2.36E-5 24 1.84E-5
13 SGTC_2034 5197015 3.96 53.1 µM 4.66E-5 SWF1 & branched chain AA biosynthesis 25 3.69E-5
14 SGTC_2180 6301957 3.95 200.0 µM 5.02E-5 RSC complex & mRNA processing 7 3.98E-5
15 SGTC_3120 9124077 3.93 49.5 µM 5.44E-5 23 4.32E-5
16 SGTC_457 grayanotoxin iii 3.85 135.0 µM 7.35E-5 74 5.89E-5
17 SGTC_2756 carbadox 3.82 75.1 µM 8.45E-5 62 6.79E-5
18 SGTC_2415 st077333 3.76 57.7 µM 1.07E-4 54 8.66E-5
19 SGTC_1204 4049-0315 3.75 18.3 µM 1.09E-4 33 8.81E-5
20 SGTC_138 0850-0259 3.73 40.9 µM 1.20E-4 cell wall 213 9.74E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.212 2.28E-35 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.185 2.25E-27 YDR335W MSN5 hom Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm
0.180 5.55E-26 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.173 5.07E-24 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.171 2.66E-23 YLR237W THI7 hom Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia
0.169 6.97E-23 YPR061C JID1 hom Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae
0.160 1.47E-20 YDR420W HKR1 hom Mucin family member that functions as an osmosensor in the Sho1p-mediated HOG pathway with Msb2p; proposed to be a negative regulator of filamentous growth; mutant displays defects in beta-1,3 glucan synthesis and bud site selection
0.157 5.32E-20 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.154 2.81E-19 YBR286W APE3 hom Vacuolar aminopeptidase Y, processed to mature form by Prb1p
0.153 4.30E-19 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.151 1.56E-18 YML017W PSP2 hom Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing
0.150 2.03E-18 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.150 2.04E-18 YNL314W DAL82 hom Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
0.149 3.25E-18 YOR051C ETT1 hom Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough
0.148 5.94E-18 YMR121C RPL15B hom Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress