HIPHOP chemogenomics database

YHL028W / WSC4

ER membrane protein involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3

Zygosity: Homozygous strain

fixedexpanded
Profile for YHL028W / WSC4

Click on Significant Values for Screen Details

Top fitness defect scores for YHL028W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2764	methyldopa	6.33	94.7 µM	5.28E-12		5	1.22E-10
2	SGTC_536	3852-0578	5.92	10.6 µM	1.04E-10		2	1.63E-9
3	SGTC_2126	5251174	5.16	33.9 µM	1.47E-8		6	1.21E-7
4	SGTC_2370	9071495	4.98	200.0 µM	4.45E-8	mitochondrial processes	9	3.19E-7
5	SGTC_1318	1280-3875	4.74	124.0 µM	1.74E-7		13	1.05E-6
6	SGTC_538	2922-0838	4.71	53.3 µM	2.17E-7	Golgi	17	1.27E-6
7	SGTC_1378	2093-0007	4.48	380.0 nM	7.69E-7		10	3.82E-6
8	SGTC_472	calcifediol	4.37	10.0 µM	1.36E-6		2	6.29E-6
9	SGTC_2848	9016370	4.01	26.0 µM	8.26E-6		21	3.03E-5
10	SGTC_2488	5268135	4.00	45.7 µM	8.49E-6		49	3.10E-5
11	SGTC_537	1334-0398	3.89	209.0 µM	1.48E-5		27	5.04E-5
12	SGTC_2629	bisabolol	3.84	100.0 µM	1.87E-5		24	6.18E-5
13	SGTC_1707	st032323	3.74	6.3 µM	2.89E-5	DNA damage response	65	9.03E-5
14	SGTC_58	0957-0393	3.67	9.0 µM	4.01E-5	cell wall	404	1.20E-4
15	SGTC_2292	7671323	3.59	168.1 µM	5.84E-5	RNA processing & uracil transport	46	1.67E-4
16	SGTC_3107	9121808	3.57	49.5 µM	6.23E-5		9	1.77E-4
17	SGTC_887	0885-0007	3.56	9.6 µM	6.46E-5	endomembrane recycling	48	1.83E-4
18	SGTC_1554	st069299	3.45	38.7 µM	1.06E-4		38	2.82E-4
19	SGTC_1539	benzyladenosine	3.34	56.0 µM	1.69E-4		30	4.23E-4
20	SGTC_1231	0242-0792	3.30	125.0 µM	1.94E-4		41	4.77E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.126	2.47E-13	YNL181W_p	YNL181W_p	het	Putative oxidoreductase; required for cell viability
0.113	5.71E-11	YDR205W	MSC2	hom	Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.113	5.62E-11	YBR120C	CBP6	hom	Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.112	7.79E-11	YBR201W	DER1	hom	Endoplasmic reticulum membrane protein, required for ER-associated protein degradation of misfolded or unassembled proteins; N- and C- termini protrude into the cytoplasm, has similarity to Dfm1p
0.112	9.47E-11	YML080W	DUS1	hom	Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus3p, and Dus4p; modifies pre-tRNA(Phe) at U17
0.093	6.46E-8	YJL132W_p	YJL132W_p	hom	Putative protein of unknown function; localizes to the membrane fraction; possible Zap1p-regulated target gene induced by zinc deficiency; YJL132W is a non-essential gene
0.093	7.37E-8	YFL056C	AAD6	hom	Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin
0.090	1.64E-7	YPR061C	JID1	hom	Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae
0.088	3.12E-7	YMR136W	GAT2	hom	Protein containing GATA family zinc finger motifs; similar to Gln3p and Dal80p; expression repressed by leucine
0.087	3.98E-7	YLR232W_d	YLR232W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene BNA5
0.086	6.06E-7	YNR060W	FRE4	hom	Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels
0.084	9.89E-7	YEL035C_p	UTR5_p	het	Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions
0.084	1.08E-6	YDR172W	SUP35	het	Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype
0.083	1.37E-6	YGR058W	PEF1	hom	Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.083	1.57E-6	YIL160C	POT1	hom	3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids