YHL028W / WSC4

ER membrane protein involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3

Zygosity: Homozygous strain
fixedexpanded
Profile for YHL028W / WSC4

Click on Significant Values for Screen Details ID:SGTC_57|Compound:miconazole|FD-Score:3.04|P-value:5.48E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:3.67|P-value:4.01E-5 ID:SGTC_80|Compound:0159-0065|FD-Score:3.04|P-value:5.43E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:-3.26|P-value:2.37E-4 ID:SGTC_311|Compound:1315-0136|FD-Score:-3.15|P-value:3.60E-4 ID:SGTC_317|Compound:4495-0002|FD-Score:-3.85|P-value:1.78E-5 ID:SGTC_337|Compound:1315-0038|FD-Score:3.27|P-value:2.22E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:3.08|P-value:4.73E-4 ID:SGTC_472|Compound:calcifediol|FD-Score:4.37|P-value:1.36E-6 ID:SGTC_536|Compound:3852-0578|FD-Score:5.92|P-value:1.04E-10 ID:SGTC_537|Compound:1334-0398|FD-Score:3.89|P-value:1.48E-5 ID:SGTC_538|Compound:2922-0838|FD-Score:4.71|P-value:2.17E-7 ID:SGTC_789|Compound:1309-1135|FD-Score:-3.09|P-value:4.52E-4 ID:SGTC_796|Compound:1234-0020|FD-Score:-3.13|P-value:3.87E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:3.13|P-value:3.91E-4 ID:SGTC_814|Compound:3342-0569|FD-Score:3.12|P-value:4.02E-4 ID:SGTC_887|Compound:0885-0007|FD-Score:3.56|P-value:6.46E-5 ID:SGTC_1025|Compound:k007-0731|FD-Score:-4.22|P-value:2.94E-6 ID:SGTC_1030|Compound:k029-0042|FD-Score:3.09|P-value:4.46E-4 ID:SGTC_1048|Compound:3161-0300|FD-Score:-3.01|P-value:6.25E-4 ID:SGTC_1178|Compound:0988-0033|FD-Score:3.20|P-value:2.93E-4 ID:SGTC_1231|Compound:0242-0792|FD-Score:3.30|P-value:1.94E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:4.74|P-value:1.74E-7 ID:SGTC_1378|Compound:2093-0007|FD-Score:4.48|P-value:7.69E-7 ID:SGTC_1484|Compound:3473-2379|FD-Score:3.05|P-value:5.29E-4 ID:SGTC_1539|Compound:benzyladenosine|FD-Score:3.34|P-value:1.69E-4 ID:SGTC_1554|Compound:st069299|FD-Score:3.45|P-value:1.06E-4 ID:SGTC_1707|Compound:st032323|FD-Score:3.74|P-value:2.89E-5 ID:SGTC_1749|Compound:st037932|FD-Score:3.06|P-value:5.05E-4 ID:SGTC_1757|Compound:st041620|FD-Score:-3.12|P-value:4.11E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-3.02|P-value:5.90E-4 ID:SGTC_1815|Compound:st051655|FD-Score:2.91|P-value:8.87E-4 ID:SGTC_1821|Compound:st053465|FD-Score:-2.95|P-value:7.78E-4 ID:SGTC_1917|Compound:st066507|FD-Score:3.21|P-value:2.85E-4 ID:SGTC_2114|Compound:5491910|FD-Score:3.25|P-value:2.42E-4 ID:SGTC_2126|Compound:5251174|FD-Score:5.16|P-value:1.47E-8 ID:SGTC_2149|Compound:5554786|FD-Score:3.13|P-value:3.82E-4 ID:SGTC_2264|Compound:7948757|FD-Score:3.16|P-value:3.51E-4 ID:SGTC_2292|Compound:7671323|FD-Score:3.59|P-value:5.84E-5 ID:SGTC_2370|Compound:9071495|FD-Score:4.98|P-value:4.45E-8 ID:SGTC_2389|Compound:9091542|FD-Score:-3.10|P-value:4.41E-4 ID:SGTC_2434|Compound:5670830|FD-Score:-3.50|P-value:8.79E-5 ID:SGTC_2435|Compound:5706476|FD-Score:3.04|P-value:5.57E-4 ID:SGTC_2488|Compound:5268135|FD-Score:4.00|P-value:8.49E-6 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:3.10|P-value:4.43E-4 ID:SGTC_2543|Compound:pimpinellin|FD-Score:2.92|P-value:8.53E-4 ID:SGTC_2554|Compound:13-methyl-4,4-bisnor-8,11,13-podocarpatrien-3-one|FD-Score:-4.24|P-value:2.70E-6 ID:SGTC_2559|Compound:tetrahydrosappanone a trimethyl ether|FD-Score:-4.08|P-value:5.91E-6 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.84|P-value:1.87E-5 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:-3.61|P-value:5.38E-5 ID:SGTC_2764|Compound:methyldopa|FD-Score:6.33|P-value:5.28E-12 ID:SGTC_2806|Compound:7952793|FD-Score:3.03|P-value:5.71E-4 ID:SGTC_2848|Compound:9016370|FD-Score:4.01|P-value:8.26E-6 ID:SGTC_2851|Compound:9015922|FD-Score:2.90|P-value:9.34E-4 ID:SGTC_2862|Compound:9027634|FD-Score:3.09|P-value:4.58E-4 ID:SGTC_2989|Compound:9073649|FD-Score:-3.01|P-value:6.25E-4 ID:SGTC_2992|Compound:9074591|FD-Score:-3.07|P-value:4.96E-4 ID:SGTC_3104|Compound:9120930|FD-Score:3.08|P-value:4.75E-4 ID:SGTC_3107|Compound:9121808|FD-Score:3.57|P-value:6.23E-5 ID:SGTC_57|Compound:miconazole|FD-Score:3.04|P-value:5.48E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:3.67|P-value:4.01E-5 ID:SGTC_80|Compound:0159-0065|FD-Score:3.04|P-value:5.43E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:-3.26|P-value:2.37E-4 ID:SGTC_311|Compound:1315-0136|FD-Score:-3.15|P-value:3.60E-4 ID:SGTC_317|Compound:4495-0002|FD-Score:-3.85|P-value:1.78E-5 ID:SGTC_337|Compound:1315-0038|FD-Score:3.27|P-value:2.22E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:3.08|P-value:4.73E-4 ID:SGTC_472|Compound:calcifediol|FD-Score:4.37|P-value:1.36E-6 ID:SGTC_536|Compound:3852-0578|FD-Score:5.92|P-value:1.04E-10 ID:SGTC_537|Compound:1334-0398|FD-Score:3.89|P-value:1.48E-5 ID:SGTC_538|Compound:2922-0838|FD-Score:4.71|P-value:2.17E-7 ID:SGTC_789|Compound:1309-1135|FD-Score:-3.09|P-value:4.52E-4 ID:SGTC_796|Compound:1234-0020|FD-Score:-3.13|P-value:3.87E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:3.13|P-value:3.91E-4 ID:SGTC_814|Compound:3342-0569|FD-Score:3.12|P-value:4.02E-4 ID:SGTC_887|Compound:0885-0007|FD-Score:3.56|P-value:6.46E-5 ID:SGTC_1025|Compound:k007-0731|FD-Score:-4.22|P-value:2.94E-6 ID:SGTC_1030|Compound:k029-0042|FD-Score:3.09|P-value:4.46E-4 ID:SGTC_1048|Compound:3161-0300|FD-Score:-3.01|P-value:6.25E-4 ID:SGTC_1178|Compound:0988-0033|FD-Score:3.20|P-value:2.93E-4 ID:SGTC_1231|Compound:0242-0792|FD-Score:3.30|P-value:1.94E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:4.74|P-value:1.74E-7 ID:SGTC_1378|Compound:2093-0007|FD-Score:4.48|P-value:7.69E-7 ID:SGTC_1484|Compound:3473-2379|FD-Score:3.05|P-value:5.29E-4 ID:SGTC_1539|Compound:benzyladenosine|FD-Score:3.34|P-value:1.69E-4 ID:SGTC_1554|Compound:st069299|FD-Score:3.45|P-value:1.06E-4 ID:SGTC_1707|Compound:st032323|FD-Score:3.74|P-value:2.89E-5 ID:SGTC_1749|Compound:st037932|FD-Score:3.06|P-value:5.05E-4 ID:SGTC_1757|Compound:st041620|FD-Score:-3.12|P-value:4.11E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-3.02|P-value:5.90E-4 ID:SGTC_1815|Compound:st051655|FD-Score:2.91|P-value:8.87E-4 ID:SGTC_1821|Compound:st053465|FD-Score:-2.95|P-value:7.78E-4 ID:SGTC_1917|Compound:st066507|FD-Score:3.21|P-value:2.85E-4 ID:SGTC_2114|Compound:5491910|FD-Score:3.25|P-value:2.42E-4 ID:SGTC_2126|Compound:5251174|FD-Score:5.16|P-value:1.47E-8 ID:SGTC_2149|Compound:5554786|FD-Score:3.13|P-value:3.82E-4 ID:SGTC_2264|Compound:7948757|FD-Score:3.16|P-value:3.51E-4 ID:SGTC_2292|Compound:7671323|FD-Score:3.59|P-value:5.84E-5 ID:SGTC_2370|Compound:9071495|FD-Score:4.98|P-value:4.45E-8 ID:SGTC_2389|Compound:9091542|FD-Score:-3.10|P-value:4.41E-4 ID:SGTC_2434|Compound:5670830|FD-Score:-3.50|P-value:8.79E-5 ID:SGTC_2435|Compound:5706476|FD-Score:3.04|P-value:5.57E-4 ID:SGTC_2488|Compound:5268135|FD-Score:4.00|P-value:8.49E-6 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:3.10|P-value:4.43E-4 ID:SGTC_2543|Compound:pimpinellin|FD-Score:2.92|P-value:8.53E-4 ID:SGTC_2554|Compound:13-methyl-4,4-bisnor-8,11,13-podocarpatrien-3-one|FD-Score:-4.24|P-value:2.70E-6 ID:SGTC_2559|Compound:tetrahydrosappanone a trimethyl ether|FD-Score:-4.08|P-value:5.91E-6 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.84|P-value:1.87E-5 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:-3.61|P-value:5.38E-5 ID:SGTC_2764|Compound:methyldopa|FD-Score:6.33|P-value:5.28E-12 ID:SGTC_2806|Compound:7952793|FD-Score:3.03|P-value:5.71E-4 ID:SGTC_2848|Compound:9016370|FD-Score:4.01|P-value:8.26E-6 ID:SGTC_2851|Compound:9015922|FD-Score:2.90|P-value:9.34E-4 ID:SGTC_2862|Compound:9027634|FD-Score:3.09|P-value:4.58E-4 ID:SGTC_2989|Compound:9073649|FD-Score:-3.01|P-value:6.25E-4 ID:SGTC_2992|Compound:9074591|FD-Score:-3.07|P-value:4.96E-4 ID:SGTC_3104|Compound:9120930|FD-Score:3.08|P-value:4.75E-4 ID:SGTC_3107|Compound:9121808|FD-Score:3.57|P-value:6.23E-5

Top fitness defect scores for YHL028W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2764 methyldopa 6.33 94.7 µM 5.28E-12 5 1.22E-10
2 SGTC_536 3852-0578 5.92 10.6 µM 1.04E-10 2 1.63E-9
3 SGTC_2126 5251174 5.16 33.9 µM 1.47E-8 6 1.21E-7
4 SGTC_2370 9071495 4.98 200.0 µM 4.45E-8 mitochondrial processes 9 3.19E-7
5 SGTC_1318 1280-3875 4.74 124.0 µM 1.74E-7 13 1.05E-6
6 SGTC_538 2922-0838 4.71 53.3 µM 2.17E-7 Golgi 17 1.27E-6
7 SGTC_1378 2093-0007 4.48 380.0 nM 7.69E-7 10 3.82E-6
8 SGTC_472 calcifediol 4.37 10.0 µM 1.36E-6 2 6.29E-6
9 SGTC_2848 9016370 4.01 26.0 µM 8.26E-6 21 3.03E-5
10 SGTC_2488 5268135 4.00 45.7 µM 8.49E-6 49 3.10E-5
11 SGTC_537 1334-0398 3.89 209.0 µM 1.48E-5 27 5.04E-5
12 SGTC_2629 bisabolol 3.84 100.0 µM 1.87E-5 24 6.18E-5
13 SGTC_1707 st032323 3.74 6.3 µM 2.89E-5 DNA damage response 65 9.03E-5
14 SGTC_58 0957-0393 3.67 9.0 µM 4.01E-5 cell wall 404 1.20E-4
15 SGTC_2292 7671323 3.59 168.1 µM 5.84E-5 RNA processing & uracil transport 46 1.67E-4
16 SGTC_3107 9121808 3.57 49.5 µM 6.23E-5 9 1.77E-4
17 SGTC_887 0885-0007 3.56 9.6 µM 6.46E-5 endomembrane recycling 48 1.83E-4
18 SGTC_1554 st069299 3.45 38.7 µM 1.06E-4 38 2.82E-4
19 SGTC_1539 benzyladenosine 3.34 56.0 µM 1.69E-4 30 4.23E-4
20 SGTC_1231 0242-0792 3.30 125.0 µM 1.94E-4 41 4.77E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.126 2.47E-13 YNL181W_p YNL181W_p het Putative oxidoreductase; required for cell viability
0.113 5.71E-11 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.113 5.62E-11 YBR120C CBP6 hom Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.112 7.79E-11 YBR201W DER1 hom Endoplasmic reticulum membrane protein, required for ER-associated protein degradation of misfolded or unassembled proteins; N- and C- termini protrude into the cytoplasm, has similarity to Dfm1p
0.112 9.47E-11 YML080W DUS1 hom Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus3p, and Dus4p; modifies pre-tRNA(Phe) at U17
0.093 6.46E-8 YJL132W_p YJL132W_p hom Putative protein of unknown function; localizes to the membrane fraction; possible Zap1p-regulated target gene induced by zinc deficiency; YJL132W is a non-essential gene
0.093 7.37E-8 YFL056C AAD6 hom Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin
0.090 1.64E-7 YPR061C JID1 hom Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae
0.088 3.12E-7 YMR136W GAT2 hom Protein containing GATA family zinc finger motifs; similar to Gln3p and Dal80p; expression repressed by leucine
0.087 3.98E-7 YLR232W_d YLR232W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene BNA5
0.086 6.06E-7 YNR060W FRE4 hom Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels
0.084 9.89E-7 YEL035C_p UTR5_p het Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions
0.084 1.08E-6 YDR172W SUP35 het Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype
0.083 1.37E-6 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.083 1.57E-6 YIL160C POT1 hom 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids