YHR014W / SPO13

Meiosis-specific protein, involved in maintaining sister chromatid cohesion during meiosis I as well as promoting proper attachment of kinetochores to the spindle during meiosis I and meiosis II

Zygosity: Homozygous strain
fixedexpanded
Profile for YHR014W / SPO13

Click on Significant Values for Screen Details ID:SGTC_2|Compound:0422-0095|FD-Score:3.50|P-value:3.15E-5 ID:SGTC_227|Compound:fluconazole|FD-Score:3.20|P-value:1.22E-4 ID:SGTC_404|Compound:fumagillin|FD-Score:3.00|P-value:2.98E-4 ID:SGTC_495|Compound:decylubiquinone|FD-Score:2.83|P-value:5.85E-4 ID:SGTC_504|Compound:lovastatin|FD-Score:-3.26|P-value:1.05E-4 ID:SGTC_588|Compound:3970-1144|FD-Score:3.07|P-value:2.24E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:3.02|P-value:2.71E-4 ID:SGTC_974|Compound:1326-0144|FD-Score:2.71|P-value:9.36E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:2.81|P-value:6.38E-4 ID:SGTC_1103|Compound:duloxetine|FD-Score:3.35|P-value:6.22E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:5.31|P-value:6.23E-10 ID:SGTC_1205|Compound:4161-2785|FD-Score:-3.12|P-value:1.91E-4 ID:SGTC_1214|Compound:0358-0031|FD-Score:2.86|P-value:5.30E-4 ID:SGTC_1301|Compound:naphtanilide LB|FD-Score:4.00|P-value:2.35E-6 ID:SGTC_1350|Compound:1495-0454|FD-Score:3.58|P-value:2.13E-5 ID:SGTC_1564|Compound:D-Arginine|FD-Score:-5.35|P-value:5.56E-10 ID:SGTC_1568|Compound:digitonin|FD-Score:3.43|P-value:4.28E-5 ID:SGTC_1574|Compound:spiramycin|FD-Score:-3.34|P-value:7.16E-5 ID:SGTC_1639|Compound:Lupinine o-methylbenzoicacid ester hydrochloride|FD-Score:2.82|P-value:6.19E-4 ID:SGTC_1761|Compound:st044296|FD-Score:-3.33|P-value:7.47E-5 ID:SGTC_1921|Compound:5740136|FD-Score:3.42|P-value:4.62E-5 ID:SGTC_1955|Compound:wogonin|FD-Score:-2.85|P-value:5.82E-4 ID:SGTC_2018|Compound:5133068|FD-Score:-3.15|P-value:1.71E-4 ID:SGTC_2023|Compound:5144124|FD-Score:-3.70|P-value:1.24E-5 ID:SGTC_2058|Compound:5236698|FD-Score:-2.99|P-value:3.31E-4 ID:SGTC_2064|Compound:5220758|FD-Score:3.47|P-value:3.57E-5 ID:SGTC_2208|Compound:7283366|FD-Score:2.94|P-value:3.84E-4 ID:SGTC_2292|Compound:7671323|FD-Score:-3.76|P-value:9.52E-6 ID:SGTC_2300|Compound:7976697|FD-Score:-2.89|P-value:5.08E-4 ID:SGTC_2421|Compound:5690543|FD-Score:3.51|P-value:2.90E-5 ID:SGTC_2488|Compound:5268135|FD-Score:2.98|P-value:3.16E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:2.74|P-value:8.56E-4 ID:SGTC_2591|Compound:skatole|FD-Score:3.11|P-value:1.83E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:-3.03|P-value:2.81E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:-3.39|P-value:5.59E-5 ID:SGTC_2639|Compound:lactacystin|FD-Score:3.13|P-value:1.67E-4 ID:SGTC_2759|Compound:disulfiram|FD-Score:3.86|P-value:5.08E-6 ID:SGTC_2764|Compound:methyldopa|FD-Score:2.98|P-value:3.22E-4 ID:SGTC_2931|Compound:9014674|FD-Score:2.94|P-value:3.80E-4 ID:SGTC_2935|Compound:9038016|FD-Score:3.17|P-value:1.39E-4 ID:SGTC_3083|Compound:9117465|FD-Score:3.33|P-value:7.04E-5 ID:SGTC_3343|Compound:9148379|FD-Score:2.73|P-value:8.97E-4 ID:SGTC_2|Compound:0422-0095|FD-Score:3.50|P-value:3.15E-5 ID:SGTC_227|Compound:fluconazole|FD-Score:3.20|P-value:1.22E-4 ID:SGTC_404|Compound:fumagillin|FD-Score:3.00|P-value:2.98E-4 ID:SGTC_495|Compound:decylubiquinone|FD-Score:2.83|P-value:5.85E-4 ID:SGTC_504|Compound:lovastatin|FD-Score:-3.26|P-value:1.05E-4 ID:SGTC_588|Compound:3970-1144|FD-Score:3.07|P-value:2.24E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:3.02|P-value:2.71E-4 ID:SGTC_974|Compound:1326-0144|FD-Score:2.71|P-value:9.36E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:2.81|P-value:6.38E-4 ID:SGTC_1103|Compound:duloxetine|FD-Score:3.35|P-value:6.22E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:5.31|P-value:6.23E-10 ID:SGTC_1205|Compound:4161-2785|FD-Score:-3.12|P-value:1.91E-4 ID:SGTC_1214|Compound:0358-0031|FD-Score:2.86|P-value:5.30E-4 ID:SGTC_1301|Compound:naphtanilide LB|FD-Score:4.00|P-value:2.35E-6 ID:SGTC_1350|Compound:1495-0454|FD-Score:3.58|P-value:2.13E-5 ID:SGTC_1564|Compound:D-Arginine|FD-Score:-5.35|P-value:5.56E-10 ID:SGTC_1568|Compound:digitonin|FD-Score:3.43|P-value:4.28E-5 ID:SGTC_1574|Compound:spiramycin|FD-Score:-3.34|P-value:7.16E-5 ID:SGTC_1639|Compound:Lupinine o-methylbenzoicacid ester hydrochloride|FD-Score:2.82|P-value:6.19E-4 ID:SGTC_1761|Compound:st044296|FD-Score:-3.33|P-value:7.47E-5 ID:SGTC_1921|Compound:5740136|FD-Score:3.42|P-value:4.62E-5 ID:SGTC_1955|Compound:wogonin|FD-Score:-2.85|P-value:5.82E-4 ID:SGTC_2018|Compound:5133068|FD-Score:-3.15|P-value:1.71E-4 ID:SGTC_2023|Compound:5144124|FD-Score:-3.70|P-value:1.24E-5 ID:SGTC_2058|Compound:5236698|FD-Score:-2.99|P-value:3.31E-4 ID:SGTC_2064|Compound:5220758|FD-Score:3.47|P-value:3.57E-5 ID:SGTC_2208|Compound:7283366|FD-Score:2.94|P-value:3.84E-4 ID:SGTC_2292|Compound:7671323|FD-Score:-3.76|P-value:9.52E-6 ID:SGTC_2300|Compound:7976697|FD-Score:-2.89|P-value:5.08E-4 ID:SGTC_2421|Compound:5690543|FD-Score:3.51|P-value:2.90E-5 ID:SGTC_2488|Compound:5268135|FD-Score:2.98|P-value:3.16E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:2.74|P-value:8.56E-4 ID:SGTC_2591|Compound:skatole|FD-Score:3.11|P-value:1.83E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:-3.03|P-value:2.81E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:-3.39|P-value:5.59E-5 ID:SGTC_2639|Compound:lactacystin|FD-Score:3.13|P-value:1.67E-4 ID:SGTC_2759|Compound:disulfiram|FD-Score:3.86|P-value:5.08E-6 ID:SGTC_2764|Compound:methyldopa|FD-Score:2.98|P-value:3.22E-4 ID:SGTC_2931|Compound:9014674|FD-Score:2.94|P-value:3.80E-4 ID:SGTC_2935|Compound:9038016|FD-Score:3.17|P-value:1.39E-4 ID:SGTC_3083|Compound:9117465|FD-Score:3.33|P-value:7.04E-5 ID:SGTC_3343|Compound:9148379|FD-Score:2.73|P-value:8.97E-4

Top fitness defect scores for YHR014W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1107 amlodipine 5.31 47.0 µM 6.23E-10 10 5.43E-8
2 SGTC_1301 naphtanilide LB 4.00 4.8 µM 2.35E-6 heme requiring 8 3.16E-5
3 SGTC_2759 disulfiram 3.86 3.4 µM 5.08E-6 38 5.74E-5
4 SGTC_1350 1495-0454 3.58 146.0 µM 2.13E-5 47 1.74E-4
5 SGTC_2421 5690543 3.51 90.7 µM 2.90E-5 29 2.22E-4
6 SGTC_2 0422-0095 3.50 29.0 µM 3.15E-5 redox potentiating 15 2.36E-4
7 SGTC_2064 5220758 3.47 200.0 µM 3.57E-5 57 2.61E-4
8 SGTC_1568 digitonin 3.43 820.0 nM 4.28E-5 sphingolipid biosynthesis & PDR1 49 3.00E-4
9 SGTC_1921 5740136 3.42 50.0 µM 4.62E-5 TRP & mitochondrial translation 81 3.18E-4
10 SGTC_1103 duloxetine 3.35 36.1 µM 6.22E-5 54 4.01E-4
11 SGTC_3083 9117465 3.33 49.5 µM 7.04E-5 45 4.42E-4
12 SGTC_227 fluconazole 3.20 33.5 µM 1.22E-4 azole & statin 126 6.77E-4
13 SGTC_2935 9038016 3.17 30.0 µM 1.39E-4 50 7.50E-4
14 SGTC_2639 lactacystin 3.13 53.0 µM 1.67E-4 65 8.65E-4
15 SGTC_2591 skatole 3.11 100.0 µM 1.83E-4 58 9.29E-4
16 SGTC_588 3970-1144 3.07 52.9 µM 2.24E-4 65 0.00109
17 SGTC_769 0302-0231 3.02 76.0 µM 2.71E-4 112 0.00126
18 SGTC_404 fumagillin 3.00 50.0 µM 2.98E-4 63 0.00136
19 SGTC_2488 5268135 2.98 45.7 µM 3.16E-4 104 0.00142
20 SGTC_2764 methyldopa 2.98 94.7 µM 3.22E-4 73 0.00144

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.138 8.41E-16 YPR079W MRL1 hom Membrane protein; has similarity to mammalian mannose-6-phosphate receptors; possibly functions as a sorting receptor in the delivery of vacuolar hydrolases; protein abundance increases in response to DNA replication stress
0.133 1.04E-14 YHR073W OSH3 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.127 1.85E-13 YMR102C_p YMR102C_p hom Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene
0.119 5.21E-12 YIL144W TID3 het Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering
0.113 5.49E-11 YMR094W CTF13 het Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis
0.109 2.37E-10 YOR174W MED4 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.108 3.03E-10 YJL195C_d YJL195C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
0.095 3.98E-8 YLR146C SPE4 hom Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid
0.092 8.38E-8 YIL067C_p YIL067C_p hom Uncharacterized protein of unknown function
0.092 9.35E-8 YIL130W ASG1 hom Zinc cluster protein proposed to function as a transcriptional regulator involved in the stress response; null mutants have a respiratory deficiency, calcofluor white sensitivity and slightly increased cycloheximide resistance
0.086 6.94E-7 YER186C_p YER186C_p hom Putative protein of unknown function
0.085 8.12E-7 YKR057W RPS21A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication
0.081 2.94E-6 YIL074C SER33 hom 3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication
0.080 3.37E-6 YML075C HMG1 hom HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress
0.080 3.59E-6 YNL031C HHT2 hom Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation