HIPHOP chemogenomics database

YHR025W / THR1

Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway

Zygosity: Homozygous strain

fixedexpanded
Profile for YHR025W / THR1

Click on Significant Values for Screen Details

Top fitness defect scores for YHR025W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_46	0329-0187a	40.40	2.2 µM	1.48E-307		1	0
2	SGTC_163	1327-0036	32.50	7.5 µM	2.33E-200		2	1.13E-232
3	SGTC_37	0566-0070	32.00	1.6 µM	7.54E-194		1	4.16E-225
4	SGTC_107	0358-0022	24.40	2.7 µM	6.90E-114	SWF1 & branched chain AA biosynthesis	2	3.69E-132
5	SGTC_2233	sulbentine	21.70	40.4 µM	1.72E-90		1	5.83E-105
6	SGTC_90	0870-0863	20.40	4.6 µM	5.08E-80		2	8.65E-93
7	SGTC_177	1327-0068	19.30	8.4 µM	9.31E-72		2	3.49E-83
8	SGTC_1195	1502-0147	17.10	41.1 µM	5.28E-57		1	4.95E-66
9	SGTC_852	0418-0143	16.40	439.0 µM	1.56E-52	calcium & mitochondrial duress	5	7.78E-61
10	SGTC_59	0330-0160	15.00	1.3 µM	3.33E-44		4	3.76E-51
11	SGTC_3059	1327-0034	14.70	3.0 µM	1.29E-42		4	2.64E-49
12	SGTC_2689	tacrolimus	14.40	100.0 µM	7.58E-41		2	3.00E-47
13	SGTC_451	chloral hydrate	13.10	100.0 µM	5.81E-34		2	3.03E-39
14	SGTC_690	glyoxal	12.30	20.3 µM	3.60E-30		11	7.74E-35
15	SGTC_2651	1327-0036	12.20	5.0 µM	6.60E-30		14	1.57E-34
16	SGTC_2430	5571206	12.20	200.0 µM	7.66E-30		6	1.86E-34
17	SGTC_371	0195-0006	12.20	7.4 µM	7.91E-30		1	1.94E-34
18	SGTC_386	1509-0011	11.90	3.1 µM	9.72E-29		34	3.57E-33
19	SGTC_3060	1327-0027	11.90	12.5 µM	1.31E-28		5	5.07E-33
20	SGTC_673	1082-0474	11.70	110.0 µM	9.15E-28	RSC complex & mRNA processing	4	4.84E-32

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.503	1.12E-214	YCR053W	THR4	hom	Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway
0.502	7.18E-213	YJR139C	HOM6	hom	Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions
0.377	6.55E-114	YER052C	HOM3	hom	Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
0.334	2.41E-88	YER086W	ILV1	hom	Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation
0.306	9.08E-74	YJR013W	GPI14	het	Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
0.264	1.06E-54	YIL052C	RPL34B	hom	Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication
0.258	3.75E-52	YBR068C	BAP2	hom	High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication
0.253	8.28E-50	YDR158W	HOM2	hom	Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
0.249	9.48E-49	YOR297C	TIM18	hom	Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex
0.232	3.54E-42	YOR184W	SER1	hom	3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress
0.226	4.63E-40	YNL229C	URE2	hom	Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion
0.216	9.60E-37	YIR008C	PRI1	het	Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
0.216	1.17E-36	YJL121C	RPE1	hom	D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress
0.213	1.00E-35	YGR208W	SER2	hom	Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source
0.210	1.15E-34	YDL040C	NAT1	hom	Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing