YHR071W / PCL5

Cyclin, interacts with and phosphorylated by Pho85p cyclin-dependent kinase (Cdk), induced by Gcn4p at level of transcription, specifically required for Gcn4p degradation, may be sensor of cellular protein biosynthetic capacity

Zygosity: Homozygous strain
fixedexpanded
Profile for YHR071W / PCL5

Click on Significant Values for Screen Details ID:SGTC_291|Compound:4130-1278|FD-Score:3.42|P-value:4.64E-5 ID:SGTC_368|Compound:fentichlor|FD-Score:-3.49|P-value:4.69E-5 ID:SGTC_374|Compound:0880-0231|FD-Score:2.78|P-value:7.34E-4 ID:SGTC_508|Compound:roscovitine|FD-Score:4.14|P-value:1.16E-6 ID:SGTC_522|Compound:1273-0010|FD-Score:2.81|P-value:6.50E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:2.84|P-value:5.76E-4 ID:SGTC_614|Compound:0462-0418|FD-Score:-4.92|P-value:1.65E-8 ID:SGTC_782|Compound:0083-0095|FD-Score:-2.78|P-value:9.55E-4 ID:SGTC_874|Compound:0599-0792|FD-Score:3.48|P-value:3.49E-5 ID:SGTC_883|Compound:0850-0245|FD-Score:4.10|P-value:1.47E-6 ID:SGTC_899|Compound:1083-0020|FD-Score:3.64|P-value:1.63E-5 ID:SGTC_980|Compound:1473-0395|FD-Score:-3.24|P-value:1.41E-4 ID:SGTC_1081|Compound:sertraline|FD-Score:2.80|P-value:6.71E-4 ID:SGTC_1215|Compound:3852-0577|FD-Score:5.08|P-value:3.61E-9 ID:SGTC_1225|Compound:1216-0074|FD-Score:3.10|P-value:1.94E-4 ID:SGTC_1227|Compound:3909-8010|FD-Score:-2.89|P-value:6.22E-4 ID:SGTC_1228|Compound:0124-0032|FD-Score:4.38|P-value:2.87E-7 ID:SGTC_1230|Compound:0170-0365|FD-Score:4.98|P-value:6.94E-9 ID:SGTC_1251|Compound:0490-5699|FD-Score:3.39|P-value:5.46E-5 ID:SGTC_1308|Compound:1187-1617|FD-Score:3.54|P-value:2.62E-5 ID:SGTC_1504|Compound:k297-0033|FD-Score:-3.18|P-value:1.85E-4 ID:SGTC_1590|Compound:st077108|FD-Score:2.93|P-value:3.92E-4 ID:SGTC_1633|Compound:st005664|FD-Score:-2.84|P-value:7.59E-4 ID:SGTC_1696|Compound:st025591|FD-Score:-3.78|P-value:1.12E-5 ID:SGTC_1726|Compound:st035556|FD-Score:2.73|P-value:8.83E-4 ID:SGTC_1738|Compound:st035555|FD-Score:-3.37|P-value:7.89E-5 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:2.88|P-value:4.78E-4 ID:SGTC_2116|Compound:5511452|FD-Score:3.26|P-value:9.60E-5 ID:SGTC_2127|Compound:5253358|FD-Score:-3.15|P-value:2.09E-4 ID:SGTC_2309|Compound:7664088|FD-Score:3.41|P-value:4.94E-5 ID:SGTC_2352|Compound:9027995|FD-Score:-3.66|P-value:2.10E-5 ID:SGTC_2563|Compound:tomatine|FD-Score:3.43|P-value:4.47E-5 ID:SGTC_2564|Compound:betulinic acid|FD-Score:3.02|P-value:2.78E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:2.75|P-value:8.19E-4 ID:SGTC_2689|Compound:tacrolimus|FD-Score:3.48|P-value:3.58E-5 ID:SGTC_2693|Compound:st077581|FD-Score:4.50|P-value:1.43E-7 ID:SGTC_2786|Compound:7743617|FD-Score:-2.88|P-value:6.33E-4 ID:SGTC_2808|Compound:7946468|FD-Score:2.81|P-value:6.46E-4 ID:SGTC_2976|Compound:9009005|FD-Score:3.28|P-value:8.98E-5 ID:SGTC_2978|Compound:9011784|FD-Score:3.99|P-value:2.70E-6 ID:SGTC_3061|Compound:glucosamine|FD-Score:2.77|P-value:7.60E-4 ID:SGTC_3215|Compound:9129942|FD-Score:4.96|P-value:8.17E-9 ID:SGTC_3297|Compound:9117502|FD-Score:2.74|P-value:8.40E-4 ID:SGTC_3326|Compound:9140064|FD-Score:-3.01|P-value:3.88E-4 ID:SGTC_291|Compound:4130-1278|FD-Score:3.42|P-value:4.64E-5 ID:SGTC_368|Compound:fentichlor|FD-Score:-3.49|P-value:4.69E-5 ID:SGTC_374|Compound:0880-0231|FD-Score:2.78|P-value:7.34E-4 ID:SGTC_508|Compound:roscovitine|FD-Score:4.14|P-value:1.16E-6 ID:SGTC_522|Compound:1273-0010|FD-Score:2.81|P-value:6.50E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:2.84|P-value:5.76E-4 ID:SGTC_614|Compound:0462-0418|FD-Score:-4.92|P-value:1.65E-8 ID:SGTC_782|Compound:0083-0095|FD-Score:-2.78|P-value:9.55E-4 ID:SGTC_874|Compound:0599-0792|FD-Score:3.48|P-value:3.49E-5 ID:SGTC_883|Compound:0850-0245|FD-Score:4.10|P-value:1.47E-6 ID:SGTC_899|Compound:1083-0020|FD-Score:3.64|P-value:1.63E-5 ID:SGTC_980|Compound:1473-0395|FD-Score:-3.24|P-value:1.41E-4 ID:SGTC_1081|Compound:sertraline|FD-Score:2.80|P-value:6.71E-4 ID:SGTC_1215|Compound:3852-0577|FD-Score:5.08|P-value:3.61E-9 ID:SGTC_1225|Compound:1216-0074|FD-Score:3.10|P-value:1.94E-4 ID:SGTC_1227|Compound:3909-8010|FD-Score:-2.89|P-value:6.22E-4 ID:SGTC_1228|Compound:0124-0032|FD-Score:4.38|P-value:2.87E-7 ID:SGTC_1230|Compound:0170-0365|FD-Score:4.98|P-value:6.94E-9 ID:SGTC_1251|Compound:0490-5699|FD-Score:3.39|P-value:5.46E-5 ID:SGTC_1308|Compound:1187-1617|FD-Score:3.54|P-value:2.62E-5 ID:SGTC_1504|Compound:k297-0033|FD-Score:-3.18|P-value:1.85E-4 ID:SGTC_1590|Compound:st077108|FD-Score:2.93|P-value:3.92E-4 ID:SGTC_1633|Compound:st005664|FD-Score:-2.84|P-value:7.59E-4 ID:SGTC_1696|Compound:st025591|FD-Score:-3.78|P-value:1.12E-5 ID:SGTC_1726|Compound:st035556|FD-Score:2.73|P-value:8.83E-4 ID:SGTC_1738|Compound:st035555|FD-Score:-3.37|P-value:7.89E-5 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:2.88|P-value:4.78E-4 ID:SGTC_2116|Compound:5511452|FD-Score:3.26|P-value:9.60E-5 ID:SGTC_2127|Compound:5253358|FD-Score:-3.15|P-value:2.09E-4 ID:SGTC_2309|Compound:7664088|FD-Score:3.41|P-value:4.94E-5 ID:SGTC_2352|Compound:9027995|FD-Score:-3.66|P-value:2.10E-5 ID:SGTC_2563|Compound:tomatine|FD-Score:3.43|P-value:4.47E-5 ID:SGTC_2564|Compound:betulinic acid|FD-Score:3.02|P-value:2.78E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:2.75|P-value:8.19E-4 ID:SGTC_2689|Compound:tacrolimus|FD-Score:3.48|P-value:3.58E-5 ID:SGTC_2693|Compound:st077581|FD-Score:4.50|P-value:1.43E-7 ID:SGTC_2786|Compound:7743617|FD-Score:-2.88|P-value:6.33E-4 ID:SGTC_2808|Compound:7946468|FD-Score:2.81|P-value:6.46E-4 ID:SGTC_2976|Compound:9009005|FD-Score:3.28|P-value:8.98E-5 ID:SGTC_2978|Compound:9011784|FD-Score:3.99|P-value:2.70E-6 ID:SGTC_3061|Compound:glucosamine|FD-Score:2.77|P-value:7.60E-4 ID:SGTC_3215|Compound:9129942|FD-Score:4.96|P-value:8.17E-9 ID:SGTC_3297|Compound:9117502|FD-Score:2.74|P-value:8.40E-4 ID:SGTC_3326|Compound:9140064|FD-Score:-3.01|P-value:3.88E-4

Top fitness defect scores for YHR071W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1215 3852-0577 5.08 11.2 µM 3.61E-9 6 1.89E-7
2 SGTC_1230 0170-0365 4.98 3.8 µM 6.94E-9 endomembrane recycling 38 3.15E-7
3 SGTC_3215 9129942 4.96 49.5 µM 8.17E-9 3 3.58E-7
4 SGTC_2693 st077581 4.50 40.7 µM 1.43E-7 20 3.37E-6
5 SGTC_1228 0124-0032 4.38 52.5 µM 2.87E-7 9 5.81E-6
6 SGTC_508 roscovitine 4.14 141.0 µM 1.16E-6 3 1.73E-5
7 SGTC_883 0850-0245 4.10 24.6 µM 1.47E-6 cell wall 29 2.08E-5
8 SGTC_2978 9011784 3.99 43.0 µM 2.70E-6 27 3.36E-5
9 SGTC_899 1083-0020 3.64 478.0 µM 1.63E-5 31 1.38E-4
10 SGTC_1308 1187-1617 3.54 41.4 µM 2.62E-5 fatty acid desaturase (OLE1) 59 2.00E-4
11 SGTC_874 0599-0792 3.48 117.0 µM 3.49E-5 42 2.50E-4
12 SGTC_2689 tacrolimus 3.48 100.0 µM 3.58E-5 70 2.55E-4
13 SGTC_2563 tomatine 3.43 8.5 µM 4.47E-5 74 3.04E-4
14 SGTC_291 4130-1278 3.42 35.5 µM 4.64E-5 Golgi 29 3.13E-4
15 SGTC_2309 7664088 3.41 163.9 µM 4.94E-5 68 3.28E-4
16 SGTC_1251 0490-5699 3.39 265.0 µM 5.46E-5 59 3.55E-4
17 SGTC_2976 9009005 3.28 19.5 µM 8.98E-5 52 5.25E-4
18 SGTC_2116 5511452 3.26 16.9 µM 9.60E-5 37 5.53E-4
19 SGTC_1225 1216-0074 3.10 20.6 µM 1.94E-4 62 9.61E-4
20 SGTC_2564 betulinic acid 3.02 100.0 µM 2.78E-4 79 0.00128

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.130 4.99E-14 YNL157W IGO1 hom Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo2p
0.128 1.01E-13 YGL081W_p YGL081W_p hom Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis
0.128 1.06E-13 YDR210W_p YDR210W_p hom Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.123 1.01E-12 YBR258C SHG1 hom Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres
0.117 9.88E-12 YBR262C AIM5 hom Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture
0.114 2.95E-11 YKL030W_d YKL030W_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene MAE1
0.110 1.57E-10 YNL274C GOR1 hom Glyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress
0.110 1.89E-10 YMR134W ERG29 het Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes
0.108 3.89E-10 YDR501W PLM2 hom Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; induced in response to DNA damaging agents and deletion of telomerase; PLM2 has a paralog, TOS4, that arose from the whole genome duplication
0.107 4.73E-10 YBL066C SEF1 hom Putative transcription factor, has homolog in Kluyveromyces lactis
0.107 5.81E-10 YCR093W CDC39 het Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
0.106 7.66E-10 YMR019W STB4 hom Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay;
0.104 1.42E-9 YJR115W_p YJR115W_p hom Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication
0.102 3.63E-9 YGR003W CUL3 hom Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3
0.100 6.24E-9 YKL171W NNK1 hom Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm