HIPHOP chemogenomics database
YHR164C / DNA2 
Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress
Zygosity: Heterozygous strain
fixedexpanded
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Top fitness defect scores for YHR164C deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Cofit Genes
Download Cofitness data (tab-delimited text) (excel)| Correlation | pval | ORF | Gene | Zygosity | Description |
|---|---|---|---|---|---|
| 0.181 | 3.10E-26 | YEL019C | MMS21 | het | SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination |
| 0.162 | 3.12E-21 | YLL011W | SOF1 | het | Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p |
| 0.156 | 1.16E-19 | YNL043C_d | YNL043C_d | hom | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W |
| 0.152 | 7.37E-19 | YGR245C | SDA1 | het | Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis |
| 0.140 | 3.92E-16 | YML046W | PRP39 | het | U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats |
| 0.132 | 1.95E-14 | YLR129W | DIP2 | het | Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex |
| 0.124 | 5.23E-13 | YKL153W_d | YKL153W_d | het | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant |
| 0.123 | 7.16E-13 | YGL074C_d | YGL074C_d | het | Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor |
| 0.121 | 2.21E-12 | YOR029W_d | YOR029W_d | hom | Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data |
| 0.119 | 4.49E-12 | YLR167W | RPS31 | het | Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog |
| 0.118 | 5.73E-12 | YGL044C | RNA15 | het | Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping |
| 0.116 | 1.47E-11 | YPL231W | FAS2 | het | Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities |
| 0.114 | 3.09E-11 | YGR074W | SMD1 | het | Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress |
| 0.111 | 1.01E-10 | YER127W | LCP5 | het | Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus |
| 0.111 | 1.11E-10 | YOL026C | MIM1 | het | Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex |