Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress

Zygosity: Homozygous strain
Profile for YHR182W / YHR182W

Click on Significant Values for Screen Details

Top fitness defect scores for YHR182W deletion by condition

RankScreen IDConditionFD ScoreConc
1 SGTC_3307 9126885 5.26 69.3 µM 4.17E-7 12 7.35E-8
2 SGTC_1046 2001-0062 4.68 216.0 µM 5.82E-6 8 1.43E-6
3 SGTC_358 0075-0058 4.67 9.4 µM 6.01E-6 34 1.49E-6
4 SGTC_3353 9151644 4.57 39.3 µM 9.15E-6 7 2.38E-6
5 SGTC_491 tetrandrine 4.52 80.3 µM 1.18E-5 6 3.16E-6
6 SGTC_1687 st020778 4.47 55.4 µM 1.45E-5 151 4.00E-6
7 SGTC_428 3448-4153 4.37 16.5 µM 2.14E-5 TSC3-RPN4 22 6.22E-6
8 SGTC_2397 5161689 4.37 200.0 µM 2.17E-5 22 6.29E-6
9 SGTC_2627 acetyl isogambogic acid 4.31 100.0 µM 2.69E-5 TSC3-RPN4 34 8.02E-6
10 SGTC_2116 5511452 3.91 16.9 µM 1.27E-4 15 4.63E-5
11 SGTC_3304 9125678 3.88 46.1 µM 1.43E-4 54 5.28E-5
12 SGTC_3301 9125354 3.87 19.8 µM 1.48E-4 16 5.46E-5
13 SGTC_1015 4130-4525 3.87 82.0 µM 1.49E-4 60S ribosome export 24 5.51E-5
14 SGTC_1170 0310-0127 3.75 64.8 µM 2.30E-4 35 9.01E-5
15 SGTC_1503 4585-0012 3.66 61.3 µM 3.12E-4 24 1.27E-4
16 SGTC_3056 9094900 3.64 49.5 µM 3.36E-4 34 1.38E-4
17 SGTC_2401 5128683 3.44 200.0 µM 6.58E-4 58 2.94E-4
18 SGTC_244 nsc-207895 3.39 1.3 µM 7.77E-4 DNA damage response 23 3.54E-4
19 SGTC_2146 5545642 3.35 200.0 µM 8.68E-4 37 4.01E-4
20 SGTC_364 0083-0097 3.31 67.7 µM 9.96E-4 49 4.68E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.131 2.93E-14 YPR177C_d YPR177C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex
0.129 6.21E-14 YGR191W HIP1 het High-affinity histidine permease, also involved in the transport of manganese ions
0.103 2.36E-9 YGL155W CDC43 het Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis
0.092 1.04E-7 YGL229C SAP4 hom Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p
0.089 2.33E-7 YPL081W RPS9A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9A has a paralog, RPS9B, that arose from the whole genome duplication
0.089 2.65E-7 YLR084C RAX2 hom N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p
0.088 3.81E-7 YER004W FMP52 hom Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation
0.087 4.14E-7 YOL105C WSC3 hom Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity; involved in the response to heat shock and other stressors; regulates 1,3-beta-glucan synthesis
0.085 9.00E-7 YMR031C EIS1 hom Component of the eisosome required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress
0.084 9.74E-7 YOR040W GLO4 hom Mitochondrial glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate
0.084 9.76E-7 YML054C CYB2 hom Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions
0.084 9.81E-7 YKL019W RAM2 het Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor
0.084 9.88E-7 YOR353C SOG2 het Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis
0.084 1.20E-6 YKL058W TOA2 het TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress
0.083 1.52E-6 YLR186W EMG1 het Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity