HIPHOP chemogenomics database

YIL048W / NEO1

Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus

Zygosity: Heterozygous strain

fixedexpanded
Profile for YIL048W / NEO1

Click on Significant Values for Screen Details

Top fitness defect scores for YIL048W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	Response Signature	Screen Rank	Screen Clearance
1	SGTC_2658	polymyxin b sulfate	77.10	77.0 µM	NEO1	1	67.80
2	SGTC_1797	st048695	75.00	56.8 µM	NEO1	1	63.50
3	SGTC_1781	st038725	73.10	66.3 µM	NEO1	1	62.00
4	SGTC_221	trifluoperazine	69.30	10.3 µM	NEO1-PIK1	1	35.70
5	SGTC_172	1112-0046	69.00	2.6 µM	NEO1	1	62.40
6	SGTC_1991	st073000	69.00	8.9 µM	NEO1	1	58.80
7	SGTC_535	2293-4129	68.10	48.2 µM	NEO1	1	61.10
8	SGTC_1760	st045453	67.60	10.4 µM	NEO1-PIK1	1	37.60
9	SGTC_1680	diphenhydramine	66.90	100.0 µM	NEO1	1	60.20
10	SGTC_270	tamoxifen	66.90	5.5 µM	NEO1	1	58.60
11	SGTC_888	0916-0002	66.00	7.0 µM	NEO1	1	61.20
12	SGTC_940	3232-1287	59.50	54.8 µM	NEO1	1	53.80
13	SGTC_1130	3454-2858	58.10	15.9 µM	NEO1	1	45.40
14	SGTC_232	clozapine	53.20	119.0 µM	NEO1	1	49.30
15	SGTC_2697	mebhydrolin	53.20	72.4 µM	NEO1	1	48.20
16	SGTC_206	4546-0025	52.30	72.0 µM	NEO1	1	43.00
17	SGTC_301	4119-0100	52.30	24.5 µM	NEO1	1	46.60
18	SGTC_1665	st014639	52.20	11.4 µM	NEO1	1	45.90
19	SGTC_2250	alverine citrate	48.10	130.0 µM		1	31.20
20	SGTC_2036	5160441	45.30	105.0 µM	NEO1	1	24.10

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.685	0	YOR039W	CKB2	hom	Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase
0.653	0	YJL154C	VPS35	hom	Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.614	0	YGL019W	CKB1	hom	Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases
0.599	0	YJL053W	PEP8	hom	Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
0.585	1.88E-306	YDR372C	VPS74	hom	Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33
0.557	2.41E-272	YDR455C_d	YDR455C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W
0.549	1.06E-263	YHR012W	VPS29	hom	Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
0.532	1.39E-244	YOR293W	RPS10A	hom	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication
0.528	1.45E-238	YDL090C	RAM1	hom	Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit
0.514	1.15E-225	YDR456W	NHX1	hom	Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion
0.506	9.01E-218	YAL009W	SPO7	hom	Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation
0.487	3.39E-199	YNL267W	PIK1	het	Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.477	1.36E-189	YHR004C	NEM1	hom	Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard
0.439	9.60E-158	YJL124C	LSM1	hom	Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress
0.425	3.30E-147	YHR108W	GGA2	hom	Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin