YIL125W / KGD1

Component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA

Zygosity: Homozygous strain
fixedexpanded
Profile for YIL125W / KGD1

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:4.57|P-value:4.82E-6 ID:SGTC_116|Compound:0155-0182|FD-Score:4.50|P-value:6.58E-6 ID:SGTC_118|Compound:0083-0097|FD-Score:3.62|P-value:2.33E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:3.72|P-value:1.58E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:3.96|P-value:6.42E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:14.00|P-value:3.00E-42 ID:SGTC_514|Compound:4466-0038|FD-Score:3.68|P-value:1.84E-4 ID:SGTC_723|Compound:4182-0001|FD-Score:3.56|P-value:2.86E-4 ID:SGTC_764|Compound:1951-2361|FD-Score:3.69|P-value:1.81E-4 ID:SGTC_803|Compound:4488-1585|FD-Score:-3.76|P-value:1.14E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:-3.22|P-value:7.83E-4 ID:SGTC_1013|Compound:4112-3890|FD-Score:5.08|P-value:4.17E-7 ID:SGTC_1140|Compound:3229-1848|FD-Score:-3.26|P-value:6.78E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:3.55|P-value:3.00E-4 ID:SGTC_1247|Compound:0448-0043|FD-Score:4.43|P-value:8.84E-6 ID:SGTC_1249|Compound:0481-0015|FD-Score:3.23|P-value:9.12E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:3.30|P-value:7.24E-4 ID:SGTC_1425|Compound:4048-4433|FD-Score:4.34|P-value:1.31E-5 ID:SGTC_1489|Compound:4065-0444|FD-Score:3.97|P-value:6.16E-5 ID:SGTC_1523|Compound:st012844|FD-Score:-3.43|P-value:3.76E-4 ID:SGTC_1526|Compound:st051518|FD-Score:4.46|P-value:7.92E-6 ID:SGTC_1635|Compound:st007035|FD-Score:4.09|P-value:3.73E-5 ID:SGTC_1700|Compound:st030840|FD-Score:-3.19|P-value:8.65E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-3.26|P-value:6.92E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:3.49|P-value:3.68E-4 ID:SGTC_2298|Compound:7976326|FD-Score:-3.53|P-value:2.68E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.70|P-value:2.68E-6 ID:SGTC_2605|Compound:digitonin|FD-Score:-3.95|P-value:5.25E-5 ID:SGTC_2694|Compound:st077685|FD-Score:3.64|P-value:2.13E-4 ID:SGTC_2801|Compound:7907800|FD-Score:4.12|P-value:3.29E-5 ID:SGTC_2802|Compound:7925815|FD-Score:3.59|P-value:2.63E-4 ID:SGTC_3183|Compound:9108214|FD-Score:-3.81|P-value:9.11E-5 ID:SGTC_3193|Compound:9111931|FD-Score:-3.96|P-value:5.14E-5 ID:SGTC_3199|Compound:9111318|FD-Score:-4.03|P-value:3.81E-5 ID:SGTC_3254|Compound:9136651|FD-Score:3.28|P-value:7.75E-4 ID:SGTC_3349|Compound:9149703|FD-Score:3.20|P-value:9.91E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:4.57|P-value:4.82E-6 ID:SGTC_116|Compound:0155-0182|FD-Score:4.50|P-value:6.58E-6 ID:SGTC_118|Compound:0083-0097|FD-Score:3.62|P-value:2.33E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:3.72|P-value:1.58E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:3.96|P-value:6.42E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:14.00|P-value:3.00E-42 ID:SGTC_514|Compound:4466-0038|FD-Score:3.68|P-value:1.84E-4 ID:SGTC_723|Compound:4182-0001|FD-Score:3.56|P-value:2.86E-4 ID:SGTC_764|Compound:1951-2361|FD-Score:3.69|P-value:1.81E-4 ID:SGTC_803|Compound:4488-1585|FD-Score:-3.76|P-value:1.14E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:-3.22|P-value:7.83E-4 ID:SGTC_1013|Compound:4112-3890|FD-Score:5.08|P-value:4.17E-7 ID:SGTC_1140|Compound:3229-1848|FD-Score:-3.26|P-value:6.78E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:3.55|P-value:3.00E-4 ID:SGTC_1247|Compound:0448-0043|FD-Score:4.43|P-value:8.84E-6 ID:SGTC_1249|Compound:0481-0015|FD-Score:3.23|P-value:9.12E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:3.30|P-value:7.24E-4 ID:SGTC_1425|Compound:4048-4433|FD-Score:4.34|P-value:1.31E-5 ID:SGTC_1489|Compound:4065-0444|FD-Score:3.97|P-value:6.16E-5 ID:SGTC_1523|Compound:st012844|FD-Score:-3.43|P-value:3.76E-4 ID:SGTC_1526|Compound:st051518|FD-Score:4.46|P-value:7.92E-6 ID:SGTC_1635|Compound:st007035|FD-Score:4.09|P-value:3.73E-5 ID:SGTC_1700|Compound:st030840|FD-Score:-3.19|P-value:8.65E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-3.26|P-value:6.92E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:3.49|P-value:3.68E-4 ID:SGTC_2298|Compound:7976326|FD-Score:-3.53|P-value:2.68E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.70|P-value:2.68E-6 ID:SGTC_2605|Compound:digitonin|FD-Score:-3.95|P-value:5.25E-5 ID:SGTC_2694|Compound:st077685|FD-Score:3.64|P-value:2.13E-4 ID:SGTC_2801|Compound:7907800|FD-Score:4.12|P-value:3.29E-5 ID:SGTC_2802|Compound:7925815|FD-Score:3.59|P-value:2.63E-4 ID:SGTC_3183|Compound:9108214|FD-Score:-3.81|P-value:9.11E-5 ID:SGTC_3193|Compound:9111931|FD-Score:-3.96|P-value:5.14E-5 ID:SGTC_3199|Compound:9111318|FD-Score:-4.03|P-value:3.81E-5 ID:SGTC_3254|Compound:9136651|FD-Score:3.28|P-value:7.75E-4 ID:SGTC_3349|Compound:9149703|FD-Score:3.20|P-value:9.91E-4

Top fitness defect scores for YIL125W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_236 1509-0011 14.00 5.3 µM 3.00E-42 118 1.42E-44
2 SGTC_1013 4112-3890 5.08 241.0 µM 4.17E-7 TSC3-RPN4 15 1.84E-7
3 SGTC_2582 agaric acid 4.70 100.0 µM 2.68E-6 redox potentiating 244 1.32E-6
4 SGTC_36 0335-0881 4.57 14.2 µM 4.82E-6 ubiquinone biosynthesis & proteasome 93 2.44E-6
5 SGTC_116 0155-0182 4.50 31.8 µM 6.58E-6 mitochondrial response to ROS 55 3.40E-6
6 SGTC_1526 st051518 4.46 75.0 µM 7.92E-6 14 4.13E-6
7 SGTC_1247 0448-0043 4.43 5.8 µM 8.84E-6 ERG2 27 4.64E-6
8 SGTC_1425 4048-4433 4.34 7.3 µM 1.31E-5 copper-dependent oxidative stress 30 7.04E-6
9 SGTC_2801 7907800 4.12 81.8 µM 3.29E-5 23 1.86E-5
10 SGTC_1635 st007035 4.09 42.4 µM 3.73E-5 204 2.13E-5
11 SGTC_1489 4065-0444 3.97 182.0 µM 6.16E-5 20 3.62E-5
12 SGTC_158 k038-0087 3.96 26.4 µM 6.42E-5 ubiquinone biosynthesis & proteasome 138 3.78E-5
13 SGTC_157 1348-1695 3.72 65.8 µM 1.58E-4 iron homeostasis 87 9.78E-5
14 SGTC_764 1951-2361 3.69 146.0 µM 1.81E-4 46 1.13E-4
15 SGTC_514 4466-0038 3.68 2.6 nM 1.84E-4 cell wall 172 1.15E-4
16 SGTC_2694 st077685 3.64 81.9 µM 2.13E-4 endomembrane recycling 40 1.35E-4
17 SGTC_118 0083-0097 3.62 135.3 µM 2.33E-4 translation 170 1.48E-4
18 SGTC_2802 7925815 3.59 54.5 µM 2.63E-4 51 1.68E-4
19 SGTC_723 4182-0001 3.56 115.0 µM 2.86E-4 35 1.84E-4
20 SGTC_1204 4049-0315 3.55 18.3 µM 3.00E-4 45 1.93E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.441 1.06E-159 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.441 3.92E-159 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.435 4.73E-155 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.423 1.05E-145 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.421 6.30E-144 YGL237C HAP2 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding
0.420 2.18E-143 YMR035W IMP2 hom Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.399 4.01E-128 YDR230W_d YDR230W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.393 2.06E-124 YDR375C BCS1 hom Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
0.392 5.67E-124 YDR518W EUG1 hom Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER
0.391 8.87E-123 YOR125C CAT5 hom Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation
0.390 2.87E-122 YPL215W CBP3 hom Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.389 1.35E-121 YJL023C PET130 hom Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.377 5.76E-114 YKL109W HAP4 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex
0.372 1.59E-110 YOR065W CYT1 hom Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex
0.370 3.36E-109 YDR148C KGD2 hom Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated